化痰通络方对急性脑梗死患者血浆同型半胱氨酸的影响

目的:观察化痰通络方治疗急性脑梗死(痰瘀闭阻证)的临床疗效及其对血浆同型半胱氨酸(Hcy)水平的影响。方法:将73例急性脑梗死患者随机分为两组,治疗组35例在常规治疗基础上加用化痰通络方治疗,对照组38例采用常规治疗,疗程均为30d;比较两组临床疗效及治疗前后血浆Hcy水平。结果:治疗组临床疗效明显优于对照组(P<0.05),Hcy水平降低程度亦优于对照组(P<0.05)。结论:化痰通络方治疗急性脑梗死(痰瘀闭阻证)疗效明显,且能明显降低血浆Hcy水平。     

化痰通络汤治疗风痰阻络型急性脑梗死临床观察

目的观察化痰通络汤治疗风痰阻络型急性脑梗死患者的临床疗效及对血同型半胱氨酸水平的影响。方法选择符合中西医诊断标准急性脑梗死患者60例,随机分为对照组和观察组各30例,对照组采用西医常规治疗,观察组在对照组治疗基础上联合化痰通络汤治疗,连续治疗14 d。于治疗前后进行美国国立卫生院卒中量表(NIHSS)评分、日常生活能力评定量表(Barthel指数)评分及血浆同型半胱氨酸(HCY)水平。结果观察组治疗后NIHSS评分、Barthel指数和血HCY水平均较治疗前明显改善(P<0.05),且改善程度优于对照组(P<0.05)。结论化痰通络汤可改善风痰阻络型急性脑梗死神经功能,降低患者致残率,改善其生活质量。     

针刺联合化痰通络汤治疗风痰瘀阻型脑梗死急性期的临床观察

目的:观察针刺与化痰通络汤联合治疗风痰瘀阻型脑梗死急性期患者的疗效。方法:选取100例风痰瘀阻型脑梗死急性期患者,随机分为观察组和对照组各50例,对照组实施常规西药治疗,观察组在对照组基础上实施针刺与化痰通络汤联合治疗,对比两组患者治疗临床效果。结果:两组治疗后的NIHSS评分、BI指数均较治疗前改善,其中观察组改善程度优于对照组(P0.05);观察组脑梗死患者治疗的总有效率明显高于对照组(P0.05)。结论:针刺与化痰通络汤联合治疗风痰瘀阻型脑梗死急性期患者疗效明显,值得临床推广。     

息风化痰通络法配合针刺对急性脑梗死患者血浆同型半胱氨酸的影响

目的观察息风化痰通络法配合针刺治疗急性脑梗死（风痰瘀血证）的疗效及其对血浆同型半胱氨酸（Hcy）含量的影响。方法将60例急性脑梗死患者随机分为两组,治疗组30例在常规治疗的基础上加用镇脑平肝丸口服及针灸治疗,对照组30例采用常规治疗,疗程均为15d;比较两组临床疗效及治疗前后神经功能缺损程度、血浆Hcy水平、血液流变学指标变化。结果治疗组临床疗效明显优于对照组,其神经功能缺损程度评分、Hcy水平、血液流变学指标改善亦优于对照组。结论息风化痰通络法配合针刺治疗急性脑梗死（风痰瘀血证）疗效明显,且能明显降低神经功能缺损程度评分、血浆Hcy含量,改善患者血液流变学指标。     

中医不同证型急性脑梗死与高同型半胱氨酸血症相关性研究

目的探讨不同中医证型急性脑梗死与高同型半胱氨酸血症(HHcy)的相关性。方法采用二元回归法对200例急性脑梗死患者的危险因素进行分析。结果中医辨证分型为肝阳暴亢型与风痰瘀阻型脑梗死患者的血同型半胱氨酸(Hcy)水平相比差异无统计学意义,但均明显高于正常值。结论 HHcy与急性脑梗死有密切的相关性,若同时合并高血压病,其危险因素明显增加。高Hcy水平对中风急性期的辨治及预防有一定的指导意义。     

大蒜素治疗伴有高同型半胱氨酸血症的脑梗死风痰瘀阻证的临床疗效评价研究

观察大蒜素治疗伴有高同型半胱氨酸血症的脑梗死风痰瘀阻证患者的疗效.方法:将符合纳入标准的患者138例分为两组,观察大蒜素治疗前后各项临床指标的变化,从而评价大蒜素的临床疗效.结果:治疗组在临床症状、体征、中医证候积分、血浆同型半胱氨酸、颈动脉内-中膜厚度的改善等方面均显著优于对照组.结论:大蒜素治疗伴高同型半胱氨酸血症的脑梗死风痰瘀阻证临床疗效显著.     

化痰通络汤联合针刺治疗中风急性期风痰阻络证临床观察

目的 探讨化痰通络汤联合穴位针刺治疗中风急性期风痰阻络证的临床疗效。方法 从瑞昌市中医医院就诊的中风急性期风痰阻络证患者中筛选64例,使用信封抽签法随机分为对照组和治疗组各32例。对照组给予常规西医和化痰通络汤治疗,治疗组于上述基础上给予穴位针刺治疗,比较2组临床疗效、中医症状积分、美国国立卫生研究院卒中量表(NIHSS)和改良Rankin量表(mRS)评分以及同型半胱氨酸(Hcy)水平。结果 治疗1周后,治疗组总有效率高于对照组(P<0.05);头晕目眩、半身不遂和言语謇涩中医症状积分、NIHSS和mRS评分以及Hcy水平均低于对照组(P<0.05)。结论 化痰通络汤联合穴位针刺治疗可改善中风急性期风痰阻络证患者中医证候和神经功能,降低Hcy水平。     

胸痹片对痰瘀互阻型不稳定性心绞痛血浆同型半胱氨酸的影响

目的:观察胸痹片对冠状动脉粥样硬化性心脏病不稳定性心绞痛(UAP)中医辨证为痰瘀互阻型且高同型半胱氨酸血症(HHCY)患者的临床疗效,探讨血浆同型半胱氨酸(Hcy)浓度与痰瘀互阻型UAP患者之间的相关性。方法:将符合纳入标准的60例(痰瘀互阻型)UAP患者随机分为对照组、治疗组各30例。对照组按照西医常规治疗;治疗组在对照组治疗基础上加用胸痹片(4片/次,每日三次)。比较两组用药前后的临床症状积分、心电图的变化情况、血浆Hcy浓度水平,结合临床进行相关分析及客观评价。结果:治疗组在疾病疗效总有效率、中医症状疗效总有效率、心电图疗效总有效率、降低血浆Hcy浓度、改善中医症状积分上均优于对照组。结论:化痰与祛瘀并举之法在UAP(痰瘀互阻型)中具有治疗价值。     

急性脑梗死中医辨证分型与同型半胱氨酸、超敏C反应蛋白水平的相关性研究

目的:探讨急性脑梗死中医辨证分型与血浆同型半胱氨酸(Hcy)、血清超敏C反应蛋白(hs-CRP)水平的关系.方法:选取210例急性脑梗死患者及30例健康者,对脑梗死患者按中医辨证分为风痰瘀阻型、阴虚风动型、气虚血瘀型、痰热腑实型、风痰火亢型、风火上扰型、痰湿蒙神型7组,分别测定急性脑梗死患者和健康对照者血浆Hey及血清hs-CRP水平,并进行组间比较.结果:急性脑梗死患者血浆Hey及血清hs-CRP水平显著高于对照组(P＜0.01),且急性脑梗死各中医证型中,风痰瘀阻型血浆Hcy水平较其他6型明显增高(P＜0.05);痰热腑实型、风痰火亢型、风火上扰型的血清hs-CRP水平较风痰瘀阻型、气虚血瘀型、阴虚风动型及痰湿蒙神型显著升高(P＜0.05).结论:血液Hcy、hs-CRP水平的升高是动脉粥样硬化性血栓性脑梗死的重要危险因素,血液Hcy、HsCRP水平可作为急性脑梗死中医辨证分型的客观指标. 健康对照者血浆Hey及血清hs-CRP水平,并进行组间比较.结果:急性脑梗死患者血浆Hey及血清hs-CRP水平显著高于对照组(P＜0.01),且急性脑梗死各中医证型中,风痰瘀阻型血浆Hcy水平较其他6型明显增高(P＜ .05);痰热腑实型、风痰火亢型、风火上扰型的血清hs-CRP水平较风痰瘀阻型、气虚血瘀型、阴虚风动型及痰湿蒙神型显著升高(P＜0.05).结论:血液Hcy、hs-CRP水平的升高是动脉粥样硬化性血栓性脑梗死的重要危险因素,血液Hcy、HsCRP水平可作为急性脑梗死中医辨证分型的客观指标. 健康对照者血浆Hey及血清hs-CRP水平,并进行组间比较.结果:急性脑梗死患者血浆He     

化痰通络汤辨证加减治疗中老年急性缺血性脑卒中(风痰瘀阻证)的疗效观察

目的:研究化痰通络汤辨证加减治疗中老年急性缺血性脑卒中(风痰瘀阻证)的疗效。方法:筛选我院收治的114例中老年急性缺血性脑卒中(风痰瘀阻证)患者,按随机数字表电脑抽取法分组,分为各为57例的两组,两组均进行西医常规治疗,观察组在此基础上进行化痰通络汤辨证加减治疗,对治疗前后两组的中医证候积分、神经功能受损程度及炎性因子水平的变化进行比较,并评定疗效。结果:两组治疗前的中医证候积分、美国国立卫生院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、C反应蛋白(C-Reactive Protein,CRP)和同型半胱氨酸(Homocysteine,Hcy)水平对比无显著差异,(P>0.05);两组治疗后的中医证候积分、NIHSS评分、CRP和Hcy水平均显著降低,且观察组的降幅更显著,(P<0.05);观察组的治疗有效率比对照组明显更优,(P<0.05)。结论:基于西医常规治疗手段下,结合化痰通络汤辨证加减治疗,可以明显改善中老年急性缺血性脑卒中(风痰瘀阻证)患者的中医证候,抑制炎性因子水平,促进神经功能恢复,提高疗效。     

Prevention of liver damage by aged garlic extract and its components in mice

The hepato-protective effects of aged garlic extract (GE), S-allyl cysteine (SAC) and S-allylmercapto cysteine (SAMC) were evaluated, using mice with acute hepatitis, induced by hepatotoxins. These substances reduced the rise of serum enzyme levels and liver necrosis caused by paracetamol or carbon tetrachloride. It was found that the protective effect of SAMC upon the liver cells were far superior than that of SAC and the other chemicals tested.     

滋阴明目丸含药血清对视网膜色素上皮细胞光损伤模型细胞凋亡率及Caspase-1、Caspase-3蛋白表达的影响

目的探讨滋阴明目丸对视网膜色素上皮(RPE)细胞光损伤后的保护作用及可能机制。方法60只SD大鼠分为滋阴明目丸低、中、高剂量组和空白组,每组15只。滋阴明目丸各剂量组分别予以滋阴明目丸浓度为0. 39、0. 78、1. 56 g/ml的混悬液灌胃,灌胃量均为34 ml/(kg·d),空白组予以生理盐水34 ml/(kg·d)灌胃,各组均给药7天。灌胃结束后制备含药血清,并采用MTT法筛选后续实验的含药血清浓度。实验分为空白组(不加含药血清)、血清组(加含药血清)、模型组(光损伤造模、不加含药血清)及中药组(光损伤造模、加含药血清)。培养24 h后模型组和中药组建立体外RPE细胞光损伤模型。检测各组细胞凋亡率,测定半胱氨酸蛋白酶1 (Caspase-1)、半胱氨酸蛋白酶3 (Caspase-3)蛋白及mRNA表达。结果中剂量血清为最佳给药剂量并用于后续实验。与空白组比较,血清组细胞凋亡率、Caspase-1和Caspase-3蛋白及mRNA表达差异均无统计学意义(P> 0. 05),模型组细胞凋亡率、Caspase-1和Caspase-3蛋白及mRNA表达显著上升(P <0. 05);与模型组比较,中药组细胞凋亡率、Caspase-1和Caspase-3蛋白及mRNA表达显著下降(P <0. 05)。结论滋阴明目丸含药血清能有效抑制RPE细胞光损伤后的细胞凋亡,可能与抑制细胞凋亡因子Caspase-1、Caspase-3蛋白及mRNA表达有关。     

电针对脑心综合征大鼠脑组织细胞凋亡及Caspase-3表达的影响

目的观察电针对脑心综合征(cerebral-cardiac syndrome,CCS)大鼠血肿周围脑组织细胞凋亡及Caspase-3表达的影响。方法从70只健康SD大鼠中随机选择10只作为假手术组,其余60只用于CCS模型复制。选取造模成功的大鼠30只,分为模型组、电针组和电针非经非穴组,每组10只。采用胶原酶加肝素联合注射大鼠尾状核方法造模,假手术组向大鼠尾状核注入等量0.9%氯化钠注射液。于造模第1天开始电针,电针组选取水沟、风府、内关和心俞穴,电针非经非穴组选取臀部非经非穴点4处,每次20min,每天1次,连续3天。假手术组和模型组仅每天抓空1次。分别采用TUNEL法和免疫组化法检测血肿周围脑组织细胞凋亡及Caspase-3表达。结果假手术组偶可见TUNEL细胞,Caspase-3少量表达;模型组血肿周围组织出现较多的TUNEL阳性细胞,Caspase-3大量表达;电针组TUNEL阳性细胞和Caspase-3表达均较模型组和电针非经非穴组明显减少(P<0.01)。结论电针可以抑制CCS大鼠脑组织细胞凋亡,其机制可能与下调血肿周围脑组织Caspase-3表达有关。     

安心颗粒对冠心病患者血浆同型半胱氨酸的影响

目的:观察安心颗粒对冠心病患者同型半胱氨酸(Hcy)的干预作用。方法:将60例冠心病患者随机分为A、B2组各30例。A组给予安心颗粒(由人参、桂枝、瓜蒌、水蛭、茯苓等组成)治疗,B组给予叶酸、维生素B12治疗。4周为1疗程。结果:A组显效10例,有效14例,无效6例,总有效率80.0％;B组显效4例,有效12例,无效14例,总有效率53.3％。2组比较,差异有显著性意义(P<0.05)。心电图改善率A组为55.5％、B组为20.0％,2组比较,差异有非常显著性意义(P<0.01)。硝酸酯类药停减率A组为88.2％,B组为27.8％,2组比较,差异有非常显著性意义(P<0.01)。治疗前2组Hcy含量均较正常对照组增高(P<0.05),治疗后Hcy含量均降低(P<0.05)。但2组治疗后比较,差异无显著性意义(P>0.05)。2组临床疗效与Hcy含量变化关系中的符合率A组为80.0％,B组为53.3％。结论:安心颗粒对冠心病患者高同型半胱氨酸血症具有与叶酸、维生素B12同样的干预作用,但在改善临床症状和心电图等方面,疗效优于叶酸、维生素B12。     

肺痿冲剂方配合大剂量N-乙酰半胱氨酸二联 治疗特发性肺间质纤维化

目的: 探讨肺痿冲剂方配合大剂量N-乙酰半胱氨酸二联治疗特发性肺间质纤维化临床效果。方法: 选取宁夏第五人民医院石炭井医院2010年2月-2012年2月收治特发性肺间质纤维化患者130例,采用随机数字表法分为对照组和观察组,每组各65例;其中对照组患者采用常规激素疗法;观察组患者采用肺痿冲剂方配合大剂量N-乙酰半胱氨酸二联疗法;比较两组患者临床治疗总有效率,胸部高分辨CT(HRCT)改变阳性率,治疗前后中医证候积分及肺功能指标等。结果: 观察组患者临床治疗总有效率明显高于对照组,组间比较差异显著(P<0.05);观察组患者HRCT检查网格影及斑片影改变阳性率均明显低于对照组,组间比较差异显著(P<0.05);经治疗后两组患者中医证候积分较治疗前均显著改善,且观察组患者中医证候积分改善程度较对照组更为明显,组间比较差异显著(P<0.05);观察组患者肺总量(TLC),用力肺活量(FVC)等肺功能指标均较对照组显著改善,组间比较差异显著(P<0.05)。结论: 肺痿冲剂方配合大剂量N-乙酰半胱氨酸二联治疗特发性肺间质纤维化与激素治疗相比可有效缓解临床症状、体征,改善肺功能,具有临床应用价值。     

药食心葆汤对心血瘀阻证冠心病患者血清同型半胱氨酸及凝血功能的影响

目的:观察药食心葆汤联合西药治疗冠心病的临床疗效及其对血清同型半胱氨酸(homocysteine,HCY)水平凝血功能的影响,并探讨其作用机制。方法:选取2015年1月至2016年1月于郑州市中医院心内科入院治疗并确诊为心血瘀阻证的冠心病患者200例,按照随机数字表法分为对照组和观察组,对照组102例,观察组98例,对照组患者采用常规西药治疗,观察组在此基础上联合药食心葆汤治疗,药物干预治疗8周,比较两组患者血清同型半胱氨酸(HCY),凝血功能[凝血酶原时间(prothrombin time,PT),部分凝血活酶时间(activated partial thromboplastin time,APTT),纤维蛋白原(fibrinogen,FIB)]等指标。结果:治疗后观察组患者凝血功能、血清HCY水平改善程度优于对照组(P0.05);观察组可明显降低CHD患者血清HCY水平(P0.05),调节患者的凝血功能(P0.05),组间比较显示两组有明显差别,特别是在改善凝血功能方面,观察组明显优于对照组(P0.05),对照组治疗前后各项指标没有统计学差异。结论:药食心葆汤联合西药治疗冠心病的临床疗效确切,能有效降低CHD患者HCY水平、调节凝血功能,值得临床推广使用。     

黄竹清脑颗粒对痰热腑实证脑出血大鼠急性期脑组织中Caspase-3表达的影响

目的:探讨黄竹清脑颗粒对痰热腑实证脑出血模型大鼠急性期脑组织中半胱氨酸蛋白酶(Caspase-3)表达的影响.方法:采用SD大鼠自体粪便灌胃配合胶原酶Ⅶ-肝素混合液立体定位注射的方法复制痰热腑实证脑出血大鼠模型,免疫组化法测定大鼠脑组织中Caspase-3表达的变化.结果:①黄竹清脑颗粒可明显减轻痰热腑实证脑出血大鼠喉...     

Oridonin induces ferroptosis by inhibiting gamma‐glutamyl cycle in TE1 cells

Oridonin (Ori) is a natural tetracyclic diterpenoid active compound with excellent antitumor activity, but the mechanism of Ori on esophageal cancer cell, TE1, remains unclear. In this study, we examined the levels of intracellular iron, malondialdehyde, and reactive oxygen species after Ori treatment, while interfering with the effects of Ori with ferroptosis inhibitor, demonstrating that Ori's inhibition of TE1 cell proliferation is associated with ferroptosis. To understand the molecular mechanism of Ori, we performed UPLC–MS/MS metabolomics profiling on TE1 cells, which show that gamma‐glutamyl amino acids (gamma‐glutamylleucine, gamma‐glutamylvaline), 5‐oxoproline, glutamate, GSH, and GSSG are changed significantly after Ori treatment. Meanwhile, the activity of gamma‐glutamyl transpeptidase 1 (GGT1) decreased. This revealed that Ori inhibited the gamma‐glutamyl cycle in TE1 cells. Furthermore, we found that Ori can covalently bind to cysteine to form the conjugate oridonin‐cysteine (Ori‐Cys), resulting in the inhibition of glutathione synthesis, which is consistent with the decrease in the enzymatic activity of glutamate cysteine ligase catalytic subunit (GCLC). Eventually, the value of intracellular GSH/GSSG was reduced, and the enzymatic activity of the glutathione peroxidase 4 (GPX4) was significantly decreased. In conclusion, our experiments indicated that Ori can inhibit the gamma‐glutamyl cycle, thereby inducing ferroptosis to exert anti‐cancer activity.     

Garlic compounds protect vascular endothelial cells from hydrogen peroxide-induced oxidant injury

Oxygen radical injury and lipid peroxidation have been suggested as major causes of cancer, atherosclerosis and the aging process. In this study, we determined in vitro the effect of aged garlic extract (AGE) and one of its components, S-allyl cysteine (SAC), on hydrogen peroxide (H₂O₂)-induced oxidant injury using bovine pulmonary artery endothelial cells (PAEC). After overnight preincubation with AGE or SAC, PAEC monolayers were exposed to H₂O₂ for 3 h. Cell viability, lactate dehydrogenase (LDH) release and lipid peroxidation were measured to assess oxidant injury. Pretreatment with AGE at 2–4 mg/mL or SAC at 4 mg/mL significantly reversed the loss of cell viability induced by 50 and 100 μm of H₂O₂. AGE or SAC also exhibited a dose-dependent inhibition of both LDH release and lipid peroxidation induced by 50 μM of H₂O₂. The results show that both AGE and SAC can protect vascular endothelial cells from oxidant injury. The data thus suggest that these compounds may be useful for retardation of the aging process and for prevention of cancer and atherosclerosis.     

Cysteine‐rich antimicrobial peptides from plants: The future of antimicrobial therapy

There has been a spurt in the spread of microbial resistance to antibiotics due to indiscriminate use of antimicrobial agents in human medicine, agriculture, and animal husbandry. It has been realized that conventional antibiotic therapy would be less effective in the coming decades and more emphasis should be given for the development of novel antiinfective therapies. Cysteine rich peptides (CRPs) are broad‐spectrum antimicrobial agents that modulate the innate immune system of different life forms such as bacteria, protozoans, fungi, plants, insects, and animals. These are also expressed in several plant tissues in response to invasion by pathogens, and play a crucial role in the regulation of plant growth and development. The present work explores the importance of CRPs as potent antimicrobial agents, which can supplement and/or replace the conventional antibiotics. Different plant parts of diverse plant species showed the presence of antimicrobial peptides (AMPs), which had significant structural and functional diversity. The plant‐derived AMPs exhibited potent activity toward a range of plant and animal pathogens, protozoans, insects, and even against cancer cells. The cysteine‐rich AMPs have opened new avenues for the use of plants as biofactories for the production of antimicrobials and can be considered as promising antimicrobial drugs in biotherapeutics.