代谢综合征对IVF/ICSI-ET助孕结局影响的临床观察

目的：探讨代谢综合征与IVF-ET/ICSI过程及结局的关系.方法：回顾性分析从2009年1月至2013年3月于我院行长方案IVF或ICSI-ET辅助助孕患者共145个周期,所有患者在进入助孕周期前均未对代谢综合征进行预处理,按照WHO制定的亚洲成人BMI标准[1]的不同分为三组：A组（正常组,18.5 kg/m2≤BMI ＜23 kg/m2）、B组（超重组,23 kg/m2≤BMI＜ 25 kg/m2）、C组（肥胖组,BMI≥25 kg/m2）;又根据2005年国际糖尿病联盟（IDF,2005年）[3]制定的代谢综合征标准降B、C各分为两组：B1组（超重非代谢综合征组）,B2组（超重代谢综合征组）,C1组（肥胖非代谢综合征组）,C2组（肥胖代谢综合征组）;比较各组患者一般情况、治疗情况及妊娠结局.结果：（1）基础情况：BMI值及腹围A、B、C三组差异显著（P＜0.01）.（2）治疗情况：Gn用量C2组均稍高于其他各组（P＜0.05）;HCG日E2值及MII期卵细胞数：B2、C2组均显著低于A组（P＜0.01）;获卵数,胚胎数B2、C1、C2组均低于A组（P＜0.05）;优质胚胎数C2组显著少于A组（P＜0.01）.（3）妊娠结局：胚胎种植率、临床妊娠率A、B1、C1组均显著高于B2及C2组（P＜0.01）;不良妊娠率B2、C2组显著高于A组（P＜0.01）,B2、C2组的不良妊娠率高于B1、C1组,但无统计学意义（P＞0.05）.结论：代谢综合征可能通过降低卵巢反应及内膜容受性来对患者产生不良影响.接受ART治疗的代谢综合征超重及肥胖患者妊娠结局明显差于正常体重患者,但超重及肥胖未伴代谢综合征的患者和正常体重患者的妊娠结局并不明显.     

In vitro Comparison between Mouse B16 and Human Melanoma Cell Lines of the Expression of ICAM-1 Induced by Cytokines and/or Hyperthermia

The expression of intercellular adhesion molecule-1 (ICAM-1) in mouse B16 and human melanoma cell lines was investigated by indirect immunofluorescence using a FACScan analyzer. Mouse B16 melanoma cell lines (B16-F1 and F10) did not express ICAM-1 under ordinary culture conditions. Neither in vitro hyperthermia at 41°C for 3 or 6 hr nor cytokines such as γ interferon (IFN-γ) or tumor necrosis factor α (TNF-α) induced ICAM-1 expression in B16 melanoma cell lines. A combination of IFN-γ with TNF-α caused slight induction of ICAM-1 expression in the B16-F10 melanoma cell line. Hyperthermia at 41°C for 3 hr in combination with the cytokines induced a slight expression of ICAM-1 in the B16-F1 melanoma cell line. Hyperthermia at 41°C for 3 hr or 6 hr did not induce de novo ICAM-1 expression but hyperthermia at 43°C for 6 hr caused rather suppression of the expression of ICAM-1 in the three human melanoma cell lines tested. In contrast, they showed a clear increase in the expression of ICAM-1 after treatment with either with IFN-γ or TNF-α, and the expression was further augmented by a combination of the two cytokines. Treatment with cytokines in combination with hyperthermia at 41°C or 43°C for 3 hr did not augment the expression of ICAM-1 over that in cytokine-treated human melanoma cell lines, at normal temperatures. Thus, it is concluded that mouse B16-F1 and F10 melanoma cell lines are different from human melanoma cell lines in terms of induction of ICAM-1 expression by cytokines and/or hyperthermia.     

Acquired C1 inhibitor deficiency: postmortem diagnosis

Acquired C1 esterase inhibitor deficiency is a clinical syndrome closely resembling hereditary angioedema in which most patients have an associated malignancy of B cell lineage. Sera from 33 patients with B cell neoplasms were assayed for C1 esterase inhibitor level by rocket immunoelectrophoresis. The clinical summary of a patient who met the biochemical criteria for acquired C1 esterase inhibitor deficiency (low C1 esterase inhibitor, low C4) is presented. We hypothesize that this syndrome represents an autoimmune reaction involving tumor cell rejections and of potential benefit to the patient.     

Effect of parent genetic background on latency and antigenicity of UV-induced tumors originating in F1 hybrids

Abstract Wide variations in susceptibility to skin tumor development by chronic ultraviolet light (UV) exposure and antigenicity of induced tumors which is estimated by tumor rejection in syngeneic recipients have been recognized among various murine strains. To examine the effect of parent genetic background on latency and antigenicity of UV-induced tumors originating in F₁ hybrids, we induced skin tumors in three mouse strains: BALB/c, C57BL/6, (B6), and C3H/HeMs (C3H/He), and their F₁, hybrids: (BALB/c×C3H/He)F₁, (CC3F₁), (BALB/c×B6)F₁, (CB6F₁) and (C3H/He×B6)F₁, (C3B6F₁) by exposing mice to UV radiation (0.44 mW/ cm² for 1 h) three times a week, and analyzed whether the UV-induccd tumors originating in F₁ hybrids possess the similar property in latency or antigenicity as seen in the UV-induced tumors derived from the parent strains. The latency of tumor induction by chronic UV exposure in C3H/He, BALB/c and their F₁, hybrid CC3F₁, was relatively short whereas that of B6 was relatively long, and that of F₁, hybrids with B6 (CB6F, and C3B6F₁) was intermediate. On the other hand, the low antigenicity as progressive growth behavior of UV-induced tumors in syngeneic recipients was observed not only in tumors derived from C3H/He but also in those from F, hybrids with C3H/He (C3B6F₁, and CC3F₁) whereas most tumors derived from B6, BALB/c and their F₁ hybrid CC3F₁ were highly antigenic as to be rejected in syngeneic recipients. These findings suggest that the parent genetic quality regulating the susceptibility to tumor induction by chronic UV exposure is co-dominantly inherited into F₁ hybrids. On the other hand, that providing the progressive growth behavior of induced tumors appears to be a dominant effect.     

不同妊娠期梅毒治疗对妊娠不良结局以及婴儿先天性梅毒的影响

目的:研究不同妊娠期梅毒治疗对妊娠不良结局以及婴儿先天性梅毒的影响。方法:选取2012年11月至2015年11月的93例妊娠期梅毒病人作为研究对象,根据治疗干预的时机不同,将其分成了三组:12周(A组),12~28周(B组),28周(C组),比较各组治疗后的妊娠不良结局、婴儿先天性梅毒发生率、RPR滴度以及1min、5min Apgar评分。结果:C组妊娠期梅毒病人的不良结局的发生率以及婴儿先天性梅毒发生率显著的高于A组和B组;C组中儿RPR滴度≥1∶8母体和婴比例均显著的高于A组和B组;A组中1min、5min Apgar评分≥7分的婴儿比例明显高于B组和C组,并且B组显著的高于C组;A组中1min、5min Apgar评分4分的婴儿比例明显低于B组和C组,并且B组显著的低于C组;上述比较差异具有显著性(P0.05)。结论:对妊娠期梅毒病人的早期干预治疗能够有效的改善不良妊娠结局,降低婴儿先天性梅毒的发生率。     

Ultra-short topical treatment of pityriasis versicolor with 2.5% bifonazole cream

The therapeutic efficacy of a preparation containing 2.5% bifonazole was investigated by comparing three different treatment modalities—A, B, and C. Group A used bifonazole only on Day 1, Group B applied the cream on Days 1, 2 and 3, and the Group C on Days 1, 3 and 5. Of the patients in Group A 56% had a negative mycological examination at the end of the study. The results obtained in Groups B and C were not significantly different: 92% of the patients had a negative mycological examination at the end of the study. Electron microscope (EM) studies showed morphological alterations such as loss of cytoplasmic organization with shrinkage and folding of the cell membranes after 1 week of treatment only in Groups B and C. We conclude that 2.5% bifonazole is a highly effective treatment for Pityrosporum ovale infection when applied using a 3-day schedule.     

中药苍柏湿毒清对小鼠阴道黏膜白介素12的影响

目的：本文旨在探讨中药苍柏湿毒清对Balb／c小鼠阴道黏膜白介素12（IL-12）浓度的影响。方法：取阴道解脲支原体培养阴性的Balb／c小鼠随机分为先干预后造模组（A1）、后造模对照组（B1）、先造模后干预组（A2）、先造模对照组（B2）、空白组（c）。A1予苍柏湿毒清水煎剂（1g生药／m1）灌胃0．2mlbid;B1予生理盐水灌胃0．2mlbid,1周后,两组皮下注射雌二醇注射液0．2mg／周,共4周。第2次注射雌二醇后,阴道注射3代8型解脲支原体茵液20ul／d,共3次,1周后补种1次,造模成功后测量阴道黏膜IL-12浓度。A2及B2组先注射雌二醇0．2mg／周,共4周,第2次注射后阴道注射uu茵液20ul／d,共3d,1周后补种1次。造模成功后进行中药水煎剂灌胃,0．2mlbid,对照组予生理盐水灌胃,0．2mlbid,共7d。干预后进行阴道黏膜白介素12浓度检测。结果：白介素12浓度先灌药后造模组与模型组比,两组有显著性差异（P〈0。05）。先干预与后干预两组比较,两组无统计学差异（P〉0．05）。先造模后灌药组与其模型组比,两组有显著性差异（P〈0．05）,而两模型组之间比较,差异不显著（P〉0．05）。干预组与空白组之间比较,均无统计学差异（P〉0．05）;同时,后造模对照组与空白组之间比较,无统计学差异（P〉0．05）,而先造模对照组与空白组间,有统计学差异（P〈0．05）。结论：苍柏湿毒清水煎剂能够使小鼠阴道黏膜白介素12的浓度恢复至正常值左右,具有修复局部炎症损伤的作用。     

Suppression of graft-versus-host reactivity in the mouse popliteal node by UVB radiation

A single exposure of recipient (C57BL6 X C3H-) F1 (B6C3F1) mice to UVB radiation suppressed the graft-versus-host (GVH) reaction to injected C3H- lymphoid cells, as measured by the popliteal lymph node weight gain assay. Several observations provided evidence to suggest that this effect of UVB radiation is nonspecific and involves an alteration of the host lymphoid cell component of the reaction. First, the nonspecific trauma of mild thermal injury also suppressed the GVH reaction. Second, although treatment of mice with rose bengal and visible radiation suppresses contact hypersensitivity while treatment with eosin and visible radiation does not, both types of phototoxic treatment suppressed the GVH reaction. Third, implantation of spleens from normal B6C3F1 mice into UVB-treated or thermally injured recipient mice at the time of injection of graft cells overcame the suppression of the GVH reaction. Finally, treatment of donor B6C3F1 mice with UVB radiation did not suppress the host-versus-graft reaction in recipient C3H- mice, which suggests that radiation does not alter the stimulatory function of B6C3F1 cells. These findings are all consistent with a hypothesis that UVB radiation suppresses GVH reactivity by reducing the host component of this immune response through diversion of cells from the site of the reaction. Thus an alteration of cell trafficking appears to be an additional pathway by which UVB radiation can produce immunosuppression.     

雌激素对白念珠菌诱导的小鼠腹腔巨噬细胞NLRP3表达的影响

目的:确定雌激素对白念珠菌诱导的小鼠腹腔巨噬细胞NLRP3炎性体基因表达的影响。方法:体外培养小鼠腹腔巨噬细胞,将其分为5组:空白对照组、白念珠菌组、白念珠菌+10~(-10)mol/L17β-雌二醇组、白念珠菌+10~(-9)mol/L 17β-雌二醇组和白念珠菌+10~(-8)mol/L17β-雌二醇组。荧光定量PCR检测细胞内NLRP3 mRNA表达;Western Blot检测细胞内NF-κBp65和caspase-1p20的活性;酶联免疫吸附法(ELISA)测定各组细胞培养上清液中白细胞介素-1β(IL-1β)的含量。结果:巨噬细胞在白念珠菌的刺激下,与空白对照组比较细胞内NLRP3 mRNA的表达、NF-κB和caspase-1p20活性和细胞培养上清液中的IL-1β含量均明显升高(P0.01);加入雌激素干预后,随着雌激素浓度的增高,细胞内NLRP3 mRNA表达、NF-κB和caspase-1p20活性、细胞培养上清液中的IL-1β含量明显低于对照组(P0.01)。结论:雌激素可能通过抑制NF-κB活性抑制白念珠菌诱导的NLRP3表达、caspase-1的活化和IL-1β的分泌。     

米卡芬净等对抗白念珠菌生物膜的体外研究

目的 探讨体外白念珠菌生物膜对米卡芬净的敏感性。方法 通过建立白念珠菌生物膜的体外模型,用抗真菌药物敏感实验法和甲基四氮盐（XTT）减低法来评价白念珠菌生物膜对氟康唑、两性霉素B、米卡芬净的敏感性。结果 30株白念珠菌生物膜中,所有菌株对氟康唑均耐药（SMIC80≥64μg／ml）;4株对两性霉素B敏感（SMIC80≤1μg／ml）,26株对两性霉素B耐药（MIC80＞1μg／ml）;27株对米卡芬净敏感（SMIC80＜ 16μg／ml）,3株对米卡芬净耐药(SMIC80≥16μg／ml)。经统计学分析,就白念珠菌生物膜的敏感性而言,米卡芬净和氟康唑之间有统计学意义（χ2=736.36, P<0.0125）,米卡芬净和两性霉素B之间有统计学意义（χ2=529.95, P<0.0125）,氟康唑和两性霉素B之间无统计学意义（χ2=2.29,P>0.0125）。结论 白念珠菌生物膜对目前常用的系统性抗真菌药物两性霉素B、氟康唑明显耐药,而对米卡芬净比较敏感。     

Molecular heterogeneity of the fourth component of complement (C4) and its genes in vitiligo.

In view of evidence suggesting vitiligo is an autoimmune disease, we investigated whether vitiligo is associated with inherited deficiencies of the fourth (C4) and second (C2) component of complement and with certain human leukocyte antigens (HLA). Analysis of functional activities of C4 and C2 in sera of patients with vitiligo (n = 42) showed that 17% of them had a heterozygous C4 deficiency and 5% had a heterozygous C2 deficiency. In the normal control group (n = 30), 3% had a heterozygous C4 deficiency and none had a C2 deficiency. C4 typing by Western blot analysis showed the frequency of the C4A*Q0 allele in the vitiligo patient group to be close to normal. However, the frequency of one C4B*Q0 allele was three times higher, and that of two C4B*Q0 alleles five times higher in the vitiligo patient group than the reported frequencies in normal control groups. Southern blot analysis of Taq1 digests of DNA using C4 and 21-hydroxylase probes showed that two patients with two C4B*Q0 alleles had a deletion of a 21-OHA-C4B segment. In the other patients, having one or two C4B*Q0 alleles, these null alleles probably occurred due to a loss of C4 gene expression. HLA analysis did not show any allelic association of C4A*Q0 or C4B*Q0 with any HLA antigen in vitiligo, but confirmed the previous findings of a negative association with HLA-DR3 and a positive association with HLA-DR4. These results suggest that abnormalities of the C4B gene and the above-mentioned associations with HLA antigens may be some of the risk factors in vitiligo.     

Effectiveness of conventional, low-dose and intermittent oral isotretinoin in the treatment of acne: a randomized, controlled comparative study

Background The efficacy of conventional isotretinoin treatment (0·5–1·0 mg kg^?1 daily for 16–32 weeks, reaching a cumulative dose of 120 mg kg^?1) for acne has been well established. To date, there are many reports regarding the efficacy of low‐dose and intermittent isotretinoin treatment in patients with acne. Data comparing these three therapeutic regimens simultaneously, however, are unavailable. Objectives To evaluate the clinical efficacy and tolerability of low‐dose and intermittent isotretinoin regimens and to compare them directly with conventional isotretinoin treatment. Methods In this study, 60 patients with moderate acne were enrolled and randomized to receive either isotretinoin at 0·5–0·7 mg kg^?1 daily (group A), isotretinoin at 0·25–0·4 mg kg^?1 daily (group B) or isotretinoin at 0·5–0·7 mg kg^?1 daily for 1 week out of every 4 weeks (group C). The total period of drug administration was 6 weeks in group C, and 24 weeks in groups A and B. Evaluations included global acne grading system (GAGS) scores, lesion counts (inflammatory and noninflammatory), patient satisfaction and side‐effects. A 1‐year follow‐up evaluation after the end of treatment was also performed. Results Differences in GAGS scores were statistically significant between groups A and C (P < 0·001) and groups B and C (P = 0·044). There was no significant difference between groups A and B. For the number of inflammatory lesions, there were statistically significant differences between groups B and C (P = 0·048) and groups C and A (P = 0·005). There was no significant difference between groups A and B. For the number of noninflammatory lesions, there were statistically significant differences between groups B and C (P = 0·046) and groups C and A (P = 0·006). There was no significant difference between groups A and B. These results suggest that the conventional and low‐dose regimens have similar efficacy. Intermittent treatment had less effect than either conventional or low‐dose treatments. Patient satisfaction was highest in group B (3·76), followed by group C (3·31), then A (3·06), with statistically significant differences between groups A and B (P = 0·003) and groups B and C (P = 0·019) but no significant difference between groups A and C. This result suggests that the low‐dose regimen is superior to other regimens (conventional or intermittent) in terms of patient satisfaction. Side‐effects were more frequent with conventional treatment compared with low‐dose and intermittent treatments. One year after the end of treatment, two of 16 patients relapsed in group A, three of 17 patients relapsed in group B, and nine of 16 patients relapsed in group C. Conclusions Our study suggests that, when considering tolerability, efficacy and patient satisfaction, low‐dose treatment is most suitable for patients with moderate acne.     

Animal model of sclerotic skin. III: Histopathological comparison of bleomycin-induced scleroderma in various mice strains

Abstract We have recently established a mouse model for scleroderma by repeated local bleomycin treatment. In this study, we compared the susceptibility to bleomycin in the development of dermal sclerosis among Balb/c, C3H/He, C57BL/6J, A/J, DBA/2, B10.BR, B10.A, and B10.D2 mouse strains. After either bleomycin or PBS treatment, skin from the injection site was histologically examined. Dermal sclerosis was induced by bleomycin treatment for 4 weeks in all of the strains examined. In particular, C3H/He, DBA/2, B10.D2 and B10.A mice developed intense dermal sclerosis characterized by deposition of homogeneous material in the dermis and thickened collagen bundles. Dermal thickness showed a more than twofold increase following bleomycin treatment, as compared with PBS treatment, except in C57BL/6J and DBA/2 mice. In A/J, C3H/He, B10.A, and B10.D2 mice, dermal thickness showed a more than 2.5-fold increase. Mast cell numbers in sclerotic skin were significantly greater than in PBS-treated skin in Balb/c and B10.A mice after 4 weeks of treatment. We also examined whether bleomycin treatment for 3 weeks could induce dermal sclerosis in C3H mice. Histological examination revealed that epidermal thickness as well as dermal sclerosis was increased in C3H mice following bleomycin treatment for 3 weeks. Increased hydroxyproline content as well as mRNA expression of α1(I) collagen, as determined by Northern blot analysis, were observed following bleomycin treatment. Taken together, we conclude that C3H/He and B10.A mouse strains are bleomycin-’susceptible’, and these strains are considered to be a suitable experimental model of bleomycin-induced scleroderma. Received: 8 June 2000 / Revised: 1 August 2000 / Accepted: 4 September 2000     

Complement and immunoglobulin deposits in the skin of patients with atopic dermatitis

The immunofluorescent patterns of uninvolved and involved skin biopsies from eight patients with atopic dermatitis were studied, using direct immunofluorescence techniques to identify deposits of the immunoglobulins G, A and M as well as the complement factors C1q, C3, C4, C5, factor B and properdin. Immunoglobulin deposits (mainly IgG) were found in five patients, complement deposits in three patients in the basement membrane zone. In three patients the immunofluorescence was positive for C3, in two patients for C1q, C4 and C5. Regarding the factors of the alternative pathway of the complement system, two patients showed deposits of properdin, one of factor B. The changes were not confined to the eczematous lesions, but were found in uninvolved skin too. The most prominent changes were observed in patients with severe disease.     

Polymorphisms in the interleukin-1 gene influence the stratum corneum interleukin-1 alpha concentration in uninvolved skin of patients with chronic irritant contact dermatitis

Background:  Interleukin (IL)-1α and its receptor antagonist IL-1ra play a role in skin inflammation. Several polymorphisms in the IL1 gene cluster, coding for IL-1α, IL-1ra, and IL-1β, influence their protein expression. Within this cluster, strong linkage disequilibrium has been shown.
Objective:  We studied the association between the polymorphisms IL1A -889 (C→T) and IL1B -31 (T→C) and the concentration of IL-1α and IL-1ra in the stratum corneum (SC).
Method:  In 124 patients with chronic irritant contact dermatitis, we genotyped the IL1A -889 and IL1B -31 polymorphisms and determined the amount of IL-1α and IL-1ra on tape strips obtained from uninvolved skin of the volar forearm.
Results:  The SC IL-1α concentration was 23% and 47% lower in subjects with IL1A -889 C/T genotype and T/T genotype, respectively, compared with wild-type genotype. In subjects with IL1B -31 C/C genotype, the IL-1α concentration was 51% lower compared with C/T and T/T genotypes. The ratio IL-1ra/IL-1α increased twofold in IL1A -889 C/T genotype and threefold in T/T genotype compared with wild type.
Conclusions:  We have shown a clear effect of IL1 genotype on protein expression in the SC. This altered expression may be responsible for the interindividual differences in the inflammatory response of the skin.     

Study of HLA class I,class II and complement genes (C2, C4A,C4B and BF) in Japanese psoriatics and analysis of a newly-found high-risk haplotype by pulsed field gel electrophoresis

Summary Genetic polymorphisms of HLA antigens and HLA-linked serum complement components (C2, C4A, C4B and BF) were investigated in 79 Japanese patients suffering from psoriasis. HLA typing revealed increased frequencies of HLA-A1, A2, B39, Bw46, Cw6, Cw7 and Cw11. Among complement components, positive associations were obtained with C4A4 and C4B2 and a negative association with BFF. The major histocompatibility complex haplotype (supratype), HLA-A2-Cw11-Bw46-C2C-BFS-C4A4-C4B2-DRw8 is purported to be a new high-risk haplotype in Japanese patients with psoriasis. Analysis of patients with this supratype via pulsed field gel electrophoresis showed the existence of specific, extensive DNA deletions near HLA-DR genes, but no disease-specific patterns could be observed by means of this technique. The newly-found high-risk haplotype indicates racial and ethnic differences among psoriatic patients.     

In vitro evaluation of concurrent use of commercially available insect repellent and sunscreen preparations

Background Insect repellents and sunscreens are over‐the‐counter products extensively used by the general public. Concurrent application of these products has become widespread in many regions across North America, because of concerns about West Nile virus and skin cancers. Objectives We investigated whether formulation type, application amount, and sequence would affect the percutaneous absorption profiles of the active repellent and sunscreen ingredients. Methods In vitro percutaneous permeation of the repellent N,N‐diethyl‐m‐toluamide (DEET) and the sunscreen oxybenzone from concurrent application of five commercially available products (A, repellent spray; B, repellent lotion; C, sunscreen lotion; D and E, combined repellent/sunscreen lotions) was measured and compared using Franz‐style diffusion cells with piglet skin at 37 °C. Results Penetration of DEET in A and B increased by 1640% and 282%, respectively, when C was applied concurrently. Penetration of DEET in D and E was 53% and 79% higher than that in B. Permeation of DEET from A + C (2 : 1) and A + C (1 : 2) increased by 530% and 278%, respectively. Permeation of oxybenzone was 189% and 280% higher in A + C and B + C than in C. Permeation of oxybenzone in D and E was also 221% and 296% higher than that in C. Permeation of oxybenzone was 196% greater when A was applied on top of C than when C was applied on top of A, while oxybenzone in A + C (1 : 2) permeated 171% more than that in A + C (2 : 1). Conclusions Concurrent application of commercially available repellent and sunscreen products resulted in significant synergistic percutaneous permeation of the repellent DEET and the sunscreen oxybenzone in vitro. The percutaneous penetration profiles were dependent upon the type of formulation, application sequence and application proportion.     

平阳霉素局部注射治疗多发性跖疣45例临床观察

目的评价平阳霉素局部注射治疗多发性跖疣的临床疗效。方法将入选的120例多发性跖疣患者随机分为A组（45例）、B组（35例）和C组（40例）。A组予平阳霉素注射治疗,B组予超脉冲CO2激光治疗,C组予液氮冷冻治疗,均为每4周1次,共治疗1-2次。结果治疗8周后,A组有效率为93.33%,B组为74.29%,C组为70.00%,A组显著优于B组和C组,差异均有统计学意义（P均〈0.05）。结论平阳霉素局部注射治疗多发性跖疣的疗效好,不良反应小,而且复发率低。     

妇产科围手术期感染的预防护理分析

目的:探究妇产科围手术期感染的预防与护理方法.方法:回顾分析我院于2010年1月~2010年9月收治的妇产科手术患者,根据其使用抗生素时期进行分组,A组于术前1~2d使用,B组于术前1~2h使用,C组于术后使用抗生素,观察其围手术期感染情况,探讨抗生素的使用时期与感染的关系、手术感染对患者性功能的影响,提出护理方案.结果:A组体温升高率、切口总感染率、体温恢复正常时间、平均住院时间均明显少于 B、C两组;三组 PFD发生率分别为19.4%、26.3%和33.3%,A组 PFD发生率明显低于 B、C两组.结论:术前1~2d行抗生素治疗对避免围手术期感染有较大意义,同时行全面的护理干预,能够有效降低妇产科围手术期的感染率.