Side-to-side and within-side variability of 3D bone microarchitecture by conventional micro-computed tomography of paired iliac crest biopsies

Bone microarchitecture in osteoporosis can be characterized by examining iliac bone biopsies and treatment effects assessed by comparing a baseline biopsy from one side to a posttreatment biopsy from the other side, a method that assumes limited side-to-side variability. New techniques based on micro-computed tomography (microCT) provide information on the three-dimensional (3D) microarchitecture of bone. We used microCT to measure side-to-side and within-side variability of 3D microarchitectural parameters of trabecular and cortical bone in paired iliac-crest biopsies, one from each side. A Bordier needle trephine was used to collect biopsies from 30 postmenopausal female cadavers (mean age, 73.7+/-10.7 years; range, 55-96 years). Biopsies were chemically defatted then imaged using a desktop microCT scanner (voxel size, 10.77 microm). Parameters measured in trabecular bone consisted of bone volume/tissue volume (BV/TV, %), direct trabecular thickness and trabecular spacing (Tb.Th and Tb.Sp, microm) using the sphere method, bone surface/bone volume (BS/BV, mm(-1)), trabecular number (Tb.N, mm(-1)), structure model index (SMI), trabecular pattern factor (Tb.Pf), and degree of anisotropy (DA). In cortical bone, we measured cortical thickness (Cort.Th), porosity (Cort.Porosity), and pore diameter (Po.Dm). For trabecular bone parameters, reproducibility as assessed from two microCT acquisitions ranged from 4.1% to 6.9%. To assess side-to-side variability, we matched the volumes of interest selected in the right and left iliac crests. The mean difference in absolute individual percent variation (mAbsDelta(ind)) between the two sides ranged from 10.8% to 14.8% for all trabecular parameters except Tb.Pf (74%) and SMI (84%). In cortical bone, mAbsDelta(ind) were 11.6% for Po.Dm, 15.1% for Cort.Porosity, and 27.6% for Cort.Th. To assess within-side variability, we divided the trabecular iliac crest volume into three equal parts, one adjacent to each cortex and one in the middle. Values of mAbsDelta(ind) versus the middle part were ranging from 7.6% for Tb.Sp to 26.2% for BV/TV. Thus, within-side variability was similar in magnitude to side-to-side variability. The considerable differences in robustness across trabecular parameters indicate a need for selecting the most stable parameters, most notably for longitudinal studies of small numbers of patients. Acquisition by microCT and image analysis must comply with stringent quality criteria, especially the distance from the cortices must be standardized.     

Histomorphometry of fresh frozen iliac crest bone biopsies

Summary Although methods are well established for the rapid histological preparation of fresh frozen soft tissues, they remain inadequate for the preservation of hard tissues. A recent technique for sectioning fresh frozen bone, in which sections can be prepared within an hour, has been applied to undecalcified human iliac crest bone biopsies. Quantitative analysis has shown that the static remodeling variables deduced from frozen sections closely resemble those derived from plastic-embedded sections prepared for routine use by established laboratory procedures. It is concluded that the rapid method is reliable for immediate diagnostic and histomorphometric purposes.     

青少年特发性脊柱侧凸患者后路髂棘处取骨植骨并发症

目的探讨青少年特发性脊柱侧凸患者后路髂棘处取骨并发症的发生率。方法对1999～2002年201例后路取髂骨行脊柱植骨融合术的青少年特发性脊柱侧凸患者进行回顾性研究。其中85例患者获得随访，最短随访时间为2年。结果住院期间有2例发生局部感染，通过灌洗引流和清创得到恢复。1例发生髂骨内板穿透，未引起临床症状。3例发生持续性疼痛，1例发生麻木。总的并发症发生率为3。5％。在随访的85例患者中，21例(24。7％)有髂棘取骨处疼痛，其中13例(15．3％)影响日常生活。7例(8．2％)需要服用非甾体类抗炎药以缓解取骨部位疼痛。6例(7.1％)瘢痕周围的皮肤感觉过敏，15例(17．6％)有瘢痕周围皮肤麻木。结论尽管青少年脊柱侧凸患者住院期间髂棘取骨处并发症较低，但经过长期随访，疼痛及麻木的并发症明显增高，应值得更多关注。     

Bone tissue composition varies across anatomic sites in the proximal femur and the iliac crest

The extent to which bone tissue composition varies across anatomic sites in normal or pathologic tissue is largely unknown, although pathologic changes in bone tissue composition are typically assumed to occur throughout the skeleton. Our objective was to compare the composition of normal cortical and trabecular bone tissue across multiple anatomic sites. The composition of cadaveric bone tissue from three anatomic sites was analyzed using Fourier transform infrared imaging: iliac crest (IC), greater trochanter (GT), and subtrochanteric femur (ST). The mean mineral:matrix ratio was 20% greater in the subtrochanteric cortex than in the cortices of the iliac crest (p = 0.004) and the greater trochanter (p = 0.02). There were also trends toward 30% narrower crystallinity distributions in the subtrochanteric cortex than in the greater trochanter (p = 0.10) and 30% wider crystallinity distributions in the subtrochanteric trabeculae than in the greater trochanter (p = 0.054) and the iliac crest (p = 0.11). Thus, the average cortical tissue mineral content and the widths of the distributions of cortical crystal size/perfection differ at the subtrochanteric femur relative to the greater trochanter and the iliac crest. In particular, the cortex of the iliac crest has lower mineral content relative to that of the subtrochanteric femur and may have limited utility as a surrogate for subtrochanteric bone. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:700–706, 2012     

Morbidity associated with anterior iliac crest bone graft harvesting in children undergoing orthopaedic surgery: a prospective review

Purpose

Autologous iliac crest bone grafting is an integral part of many orthopaedic surgical procedures. Several studies have documented morbidity and prolonged pain following iliac crest bone graft harvesting in adults; however, in children there is a paucity of information. The purpose of the present study was to quantify the degree of pain and morbidity associated with anterior iliac crest graft harvesting in children undergoing non-spinal orthopaedic surgery.

Methods

Patients were prospectively enrolled prior to orthopaedic surgery. A patient self-reported visual analogue score was used to record pain at specified time points following surgery. In addition, the patients were reviewed at 2 and 6 weeks, 3 months and 1 year after surgery to record any complications.

Results

Data was collected on 33 patients (34 graft sites). Only one patient (2.94 %) had a complication, namely an injury to the lateral femoral cutaneous nerve. This resolved 3 months after surgery. 89 % of patients had no pain at the iliac crest graft harvest site 3 months after surgery. The three patients who had pain at 3 months had visual analogue scores of 1.0, 1.1 and 1.3, respectively.

Conclusion

This series reveals a very low complication rate and minimal iliac crest graft harvest site pain in children undergoing non-spinal orthopaedic surgery. In addition, the pain experienced is short-lived.     

Stromal cell activity in bone marrow from the tibia and iliac crest of patients with rheumatoid arthritis

Bone marrow aspirates were obtained from the iliac crest and tibial epiphysis in 23 patients with rheumatoid arthritis (RA) who were undergoing total knee arthroplasty. The number of fibroblast colony-forming units (CFU-F), which contain osteogenic precursor cells, and alkaline phosphatase (ALP) activity, as a marker of the osteoblastic phenotype, were compared between the iliac and tibial marrow for each patient. The prevalence of CFU-F in tibial marrow was similar to that in iliac marrow (96% vs 100%, respectively). However, the average number of CFU-F per 4 × 10⁵ bone marrow mononuclear cells was significantly lower in tibial marrow than in iliac marrow (8.2 vs 28.1, respectively; P < 0.01). Although ALP activity was detected in all iliac and tibial marrow specimens, it was significantly lower in tibial marrow compared with iliac marrow (3.7 vs 11.9 nmol/min/mg protein, respectively; P < 0.01). In addition, there was a significant correlation between the patient's age and the number of CFU-F in iliac marrow (r = −0.547; P < 0.01), although there was no correlation in tibial marrow. These results demonstrate that the osteogenic activity of bone marrow varies at different sites in patients with RA. The data may also contribute to further investigation into the differential effects of various disease processes on systemic as well as local stromal cell activity in bone marrow. Received: April 18, 2000 / Accepted: July 11, 2000     

The relationship between spinal trabecular bone mineral content and iliac crest trabecular bone volume

Summary The relationship between spinal trabecular bone mineral density and iliac crest trabecular bone volume has been studied in 84 patients, 23 with primary osteoporosis, 19 with osteoporosis secondary to inflammatory bowel disease, and 42 with nonsteroid-treated rheumatoid arthritis. Spinal trabecular bone mineral density was measured in the first three lumbar vertebrae by quantitative computed tomography, and iliac crest trabecular bone volume was assessed histomorphometrically in sections from trans-iliac biopsies using computerized techniques. In all 84 patients, there was a significant positive correlation between the two measurements (r=0.60,P<0.001). However, when the three patient groups were analyzed separately, a significant correlation was found in the group with secondary osteoporosis (r=0.65,P<0.01) but not in the patients with primary osteoporosis (r=0.07) or rheumatoid arthritis (r=0.19). These results indicate that the relationship between spinal trabecular bone mineral density and iliac crest trabecular bone volume differs according to the underlying disease process, these differences possibly reflecting variations in skeletal patterns of bone loss in different types of osteoporosis.     

Nondestructive determination of iliac crest cancellous bone strength by pQCT

The close relationship between apparent bone density and compressive strength is well established. In clinical situations, histomorphometry, and determination of the compressive strength on bone biopsies are destructive methods and require two separate biopsies from each patient. The aim of this study was to evaluate whether volumetric bone density measured by peripheral quantitative computed tomography (pQCT) could be used as a nondestructive method for estimating trabecular bone strength of iliac crest bone biopsies, thereby allowing the same biopsy to be used for subsequent histomorphometry. Materials consisted of trabecular bone samples prepared from unilateral transiliac crest bone samples obtained at autopsy [total 95 specimens; 41 females (21–90 years) and 54 males (23–87 years)]. From these, the apparent density of the cancellous bone was evaluated by pQCT in a 1-mm-thick slice in the middle of the biopsy and also by ash density measurement. Bone strength was measured by compression test. A strong power relationship was found between density measured by pQCT and compressive strength (r = 0.93, p < 0.00001). Likewise, there was a strong power relationship between ash density and compressive strength (r = 0.97, p < 0.00001). A linear correlation was found between pQCT measurement and ash density (r = 0.98, p < 0.00001), indicating a very high accuracy for the pQCT measurement. In conclusion, pQCT provides a very good estimate of cancellous bone strength. This nondestructive assessment of strength of iliac crest bone biopsies thereby enables biomechanical information as well as histomorphometric measurements to be obtained from the same biopsy.     

Trabecular architecture in iliac crest bone biopsies: infra-individual variability in structural parameters and changes with age

A histomorphometric study was conducted on bilateral iliac crest samples obtained at autopsy from 27 subjects who had died suddenly. Six parameters related to cancellous bone structure were measured: bone volume (BV/TV), surface density (BS/TV), surface/volume ratio (BS/BV), trabecular thickness (Tb.Th.), trabecular number (Tb.N), and trabecular separation (Tb.Sp). There were no significant differences between right and left sides in the mean values for each parameter. However, when subjects were considered individually, there was a substantial difference in the majority of cases for all parameters. The intra-individual variation (IIV) was calculated for each subject as the percentage deviation from the mean for the two sides. There was a wide range in IIV (0.05-30.27%) with a mean value of approximately 11.5% for each parameter. In males the mean IIV ranged from 9 to 11% and from 14 to 16% in females. The IIV in BV/TV was positively correlated with age. Data generated on a subsample of 15 males were used to predict patient group sizes required to detect minimum significant differences in studies involving repeat biopsies. Sample sizes of 32, 16, and 8 patients would be required for relative increments in BV/TV of 29, 36, and 46%, respectively, to be statistically significant. Tb.Sp increased significantly with age but there was no significant change in Tb.Th. This supports the view that bone loss with aging occurs primarily through a mechanism involving complete disappearance of individual trabecular plates.     

Continuous infusion of ropivacaïne: an optimal postoperative analgesia regimen for iliac crest bone graft in children

Background: Postoperative analgesia of iliac crest (IC) donor site can be performed by on site infiltrations of local anesthetics (LA) or morphine. Single injections or continuous infusions of LA proved their efficacy in adults, but was not reported in children. We prospectively evaluated the interest of a continuous infusion of 0.2% ropivacaïne at the IC donor site in terms of postoperative pain relief and rescue analgesics consumption. Methods: Sixteen consecutive patients, aged from 4 to 16 years scheduled for maxillar alveolar graft with IC bone, were included. After IC bone graft surgery under general anesthesia, they received a 0.2–0.4 ml·kg^?1 bolus of 0.2% ropivacaïne through the IC catheter; then a continuous infusion of 0.2% ropivacaïne at 0.125 ml·kg^?1·h^?1 was administrated for 48 h with disposable elastomeric pumps. Children systematically received paracetamol (15 mg·kg^?1 four times a day) and niflumic acid (40 mg·kg^?1 twice a day). Postoperative pain was evaluated using a Visual Analog Scale (>7 years old) or Children and Infants Postoperative Pain Score (between 4 and 7 years old) every 4 h until H48. Doses of rescue analgesics and adverse events (LA toxicity, catheter’s removal, nausea–vomiting) were also noted. Three months after surgery a blinded clinical research assistant reviewed all children and assessed functional recovery, neuropathic chronic pain symptoms or local complications. Results: The median value of IC graft pain scores was 0 during whole studied period. Of the patients, 31.2% did not require any rescue analgesics and 43.8% needed only once. No adverse events related to LA and no removal of catheter were noted. One child had nausea in the 48‐h postoperative period, and one child had neuropathic pain symptoms at 3 months at the donor site. Conclusion: Continuous infusion of 0.2% ropivacaïne through an IC catheter is an optimal and safe technique of regional postoperative analgesia after bone graft harvest in children.     

超声引导下腹横筋膜平面阻滞用于髂嵴前部取骨移植术的效果

目的 探讨超声引导下腹横筋膜平面(TFP)阻滞在髂嵴前部取骨移植术的应用效果。
方法 选择行髂嵴前部取骨移植术患者59例,男32例,女27例,年龄18～64岁,BMI 18～24 kg/m²,ASA Ⅰ或Ⅱ级。将患者随机分为两组：超声引导下TFP阻滞联合全麻组（T组,n=30）和常规全麻组（G组,n=29）。T组行超声引导下术侧TFP阻滞,G组不进行TFP阻滞。记录麻醉诱导前1 min、手术开始后1、10、30、60 min和手术结束时的HR、MAP和呼出气七氟醚浓度（C_ETSev）、术中芬太尼、瑞芬太尼和丙泊酚用量、拔管时间、PACU停留时间。记录术后4、8、12、24、48 h髂骨供区的静息和活动时VAS疼痛评分。记录PCIA总按压次数、有效按压次数、曲马多补救镇痛用量、补救镇痛和患者对髂骨供区镇痛的满意情况。
结果 与G组比较,手术开始后1、10、30 min T组HR明显减慢、MAP明显降低（P＜0.05）,手术开始后1 min至手术结束时T组C_ETSev明显降低（P＜0.05）;T组术中芬太尼、瑞芬太尼和丙泊酚用量明显减少（P＜0.05）,拔管时间和PACU停留时间明显缩短（P＜0.05）;T组术后4、8、12 h的静息和活动时VAS疼痛评分、术后PCIA总按压次数、有效按压次数、曲马多补救镇痛用量明显减少（P＜0.05）、补救镇痛率明显降低（P＜0.05）,患者对髂骨供区镇痛满意率明显升高（P＜0.05）。
结论 超声引导下TFP阻滞用于髂嵴前部取骨移植术麻醉和镇痛效果较好,有利于减少围术期麻醉镇痛药用量,血流动力学平稳,恢复快速,安全性高,患者满意度高。     

脊柱前路手术髂前嵴取骨并发症相关分析

目的:探讨脊柱前路手术髂前嵴取骨的并发症发生情况。方法:回顾性分析2001年1月-2005年9月取髂骨行脊柱植骨融合术的154例患者,取骨部位均为髂前嵴,统计供骨区并发症,分析其相关因素。结果:术后随访时间均超过1年。28例(18.2%)患者发生不同程度的并发症,其中14例(9.1%)出现轻度并发症,12例(7.8%)出现中度并发症,2例(1.3%)出现重度并发症。127例(82.5%)患者接受了问卷调查,其中17例(13.4%)患者诉取骨处疼痛,疼痛自我感觉评分为1-6分(平均2分),其中3例(2.4%)患者需服用非甾体类抗炎药以减轻疼痛;7例(5.5%)患者诉取骨处有麻木感;无患者诉取骨处的疼痛及麻木感影响其日常活动;122例(96.1%)患者对供骨区切口外观满意。结论:髂前嵴取骨并发症中供骨区疼痛及麻木发生率较高,尤其是疼痛,应引起足够的重视。     

Frequency of adynamic bone disease and aluminium storage in Italian uraemic patients--retrospective analysis of 1429 iliac crest biopsies

BACKGROUND.: Adynamic bone disease was initially attributed to aluminiumintoxication in association with low circulating levels of parathyroidhormone. More recently adynamic bone disease has been describedeven in the absence of aluminium intoxication. PURPOSE OF THE STUDY.: It was the purpose of this retrospective analysis of 1429 iliaccrest biopsies sent to our laboratory from 1985 to 1994 by 41Italian nephrology and dialysis centres to assess the frequencyof adynamic bone disease and aluminium accumulation. METHODS.: Adynamic bone disease was diagnosed by histological and histodynamic(tetracycline labelling) analysis, on the basis of predeterminedcriteria. Aluminium accumulation was assessed by aluminon histochemicalstaining. RESULTS.: The frequency of adynamic bone disease was fairly constant atapproximately 15% from 1985 to 1994. In contrast, aluminiumaccumulation, defined as positive aluminon histochemical staining,decreased during the same period from 36% to 4%. CONCLUSIONS.: Our data clearly show a dissociation of the incidence of adynamicbone disease and aluminium accumulation in bone. At least today,given the low prevalence of aluminium intoxication, factorsother than aluminium are the main cause of adynamic bone disease.     

Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells: relationship to donor age, bone volume, and concentration of TGF-β in human bone matrix in vivo

Transforming growth factor-β (TGF-β) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-β levels in primary bone cell cultures from the iliac crest of 112 women, aged 28–79 years. TGF-β1 was the major TGF-β isoform in the conditioned media, as determined by neutralizing TGF-β activity with specific antibodies against TGF-β1–3 in the mink lung cell bioassay, and by enzyme-linked immunoassay (ELISA). TGF-β1 levels in the conditioned media did not change with donor age. There was a lack of association between TGF-β levels in vitro and the concentration of matrix-associated TGF-β in vivo. TGF-β1 levels failed to be associated with the local trabecular bone volume in the complete study population (r = +0.15, p = 0.16, n = 89). A significant association between TGF-β1 levels and bone volume was present in premenopausal women (r = +0.39, p = 0.02, n = 33), but was largely accounted for by the two samples with the highest TGF-β concentrations. In conclusion, our data suggest that TGF-β1 is the major TGF-β isoform produced by human bone cells in vitro, and that the constitutive secretion of TGF-β by bone cells does not change with age. Whether constitutive differences in TGF-β secretion may be a determinant of human bone mass remains to be clarified in further studies.     

Histomorphometric study of trabecular channels in normal iliac bone

Summary Cancellous bone channels in the normal iliac crest have been studied histologically and by histomorphometry, and their biological role has been considered. Eighty percent of trabecular channels were typical osteons with the same structural and remodeling features as cortical osteons. The similarity of osteons in these two locations was corroborated by the comparability of morphometric features. The points of difference between osteons in the two types of bone were irregular configurations of trabecular osteons and marrow cells in the central canal in some. Since the number of trabecular osteons decreases with age, and since active trabecular resorption cavities were few in number, it is unlikely that additional osteons are formed in normal bone after the active modeling phase of bone growth. It is improbable that they make a significant contribution to bone remodeling since their number decreases with age, and since the available surface of trabecular channels for remodeling is extremely small. However, it is probable that they aid the intraosseous microcirculation and mineral exchange in thick trabecula and bifurcations, where they are mainly located. The demarcation curves at the 95% confidence interval, which suggest the normal range for numbers of channels, was calculated from the scatter diagram against bone area.     

Use of tricortical iliac crest strut autograft in comminuted posterior wall acetabular fractures: A case series

Purpose

The results for fixation of comminuted posterior wall acetabular fractures are not very promising with reported complications in terms of osteoarthritis, nonunion and malunion which subsequently require conversion to total hip arthroplasty. The conversion to total hip arthroplasty is possible in patients over 50 years of age but not in younger patients. So this requires new methods for salvage of the native hip in young patients.

Arthroscopic reconstruction of chronic anteroinferior glenoid defect using an autologous tricortical iliac crest bone grafting technique

Only a few reports exist on the management of severe anteroinferior glenoid defects in case of recurrent shoulder instability most of them including open approaches. We describe an all-arthroscopic reconstruction technique of the anteroinferior glenoid that includes an autologous iliac crest bone grafting using bio-compression screws and a capsulolabral repair using suture anchors. This technique recreates the bony and soft-tissue anatomy of the anteroinferior glenoid while preserving the integrity of insertion of the subscapularis (SSC) tendon.     

A novel technique for the repair of lumbar hernias after iliac crest bone harvest

BACKGROUND: Lumbar hernia is a clinical entity that has been increasingly more common since the advent of iliac bone harvest for bone grafting procedures. These can be very technically difficult to repair and have a high recurrence rate. METHODS: Using a corkscrew anchor suture device, we have developed a novel and simple way to repair these hernias with no recurrence. Here we present our experience with the corkscrew suture anchor device. RESULTS: This technique has been performed in 2 patients at our institution, and in both cases, the hernia was successfully repaired. Our 1-year follow-up on this technique demonstrates intact repairs with no sign of recurrence. CONCLUSIONS: The placement of corkscrew suture anchors along the iliac crest remnant is a simple technique requiring minimal bony exposure. The anchors facilitate the long-term fixation of mesh despite the lack of fascia in this area. We conclude that this is a simple and effective approach for repair of these challenging hernias.