可溶性白细胞介素2受体与类风湿关节炎

可溶性白细胞介素２受体与类风湿关节炎钱孝贤余步云采用双抗体夹心酶联免疫吸附试验（ＥＬＩＳＡ）检测了４２例类风湿关节炎（ＲＡ）患者血清中可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）水平，分析了其与临床表现及其他化验结果之间的关系，并观察治疗前后血清ｓＩＬ－２...     

胃癌患者血清可溶性白细胞介素－2受体初步研究

应用双抗体夹心ＥＬＩＳＡ法对３０例胃癌患者血清ｓＩＬ－２Ｒ水平进行测定。结果表明，胃癌患者血清ｓＩＬ－２Ｒ水平明显高于正常对照（Ｐ＜０．０１）及慢性良性胃病患者（Ｐ＜０．０５）；Ⅲ－Ⅳ期患者血清ｓＩＬ－２Ｒ水平显著高于Ⅰ－Ⅱ期患者（Ｐ＜０．０５）；低度分化者较中度分化者ｓＩＬ－２Ｒ水平明显升高（Ｐ＜００１）；贲门癌患者ｓＩＬ－２Ｒ水平较胃窦（体）癌者高，但未达到统计学差别（Ｐ＞０．０５）；胃癌根治术后一周血清ｓＩＬ－２Ｒ水平较术前升高（Ｐ＜０．０１），术后二周血清ｓＩＬ－２Ｒ较术前明显降低（Ｐ＜０．０１），与正常人相比无明显差异（Ｐ＞００５）。结果提示血清ｓＩＬ－２Ｒ测定可作为胃癌患者病情判断、观察疗效及估价预后新的生物学指标。     

慢性充血性心力衰竭患者血清可溶性白细胞介素—2受体水平变化

为了研究慢性充血性心力衰竭患者血清可溶性白细胞介素－２受体的变化，对４６例心衰患者进行测定发现，其血清ｓＩＬ－２Ｒ水平明显高于对照组，且随着心衰加ｓＩＬ－２４水平增高；不同病因组中，肺心病，扩张型心肌病患者ｓＩＬ－２Ｒ水平明显高于风心病和冠心病组，说明心衰时存在免疫功能异常，且ｓＩＬ－２Ｒ的变化与病因和心功能均有关。     

胃癌患者血清可溶性IL—2受体T细胞亚群及NK细胞的临床 …

目的 较全面地观察胃癌虱的细胞免疫功能,为胃癌的预测,诊断,病情估计及免疫治疗提供依据。方法 应用酶联免疫双抗夹心法（ＥＬＩＳＡ）及碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）桥联酶标法,检测３４例胃癌患者与对照者血清可溶性白细胞介素－２受体（ｓＩＬ－２Ｒ）,Ｔ淋巴细胞亚群及自然杀伤（ＮＫ）细胞。结果 胃癌患者ｓＩＬ－２Ｒ水平明显高于对照组,Ⅲ,Ⅳ期高于Ⅰ,Ⅱ期（Ｐ〈０．０１）;ＣＤ４,ＣＤ８,ＣＤ４／Ｃ     

肺癌患者手术前后血清IL—2和可溶性IL—2受体含量的变化及临床 …

为了解老年肺癌患者的免疫状况，本文采用ＲＩＡ和ＥＬＩＳＡ法分别测定老年肺癌病人表中ＩＬ－２和ＳＩＬ－２Ｒ含量，结果显示术前ＩＬ－２含量明显低于正常对照组而ＳＩＬ－２Ｒ含量明显高于正常对照组。肺癌术后ＩＬ－２高于术前而ＳＩＬ－２Ｒ明显低于术前。提示此两项免疫指标对评价部效和观察预后有一定的临床意义。     

血清可溶性白细胞介素2受体及T淋巴细胞亚群联合检测对丙型肝…

本文检测了６８例丙型肝炎患者的ＳＩＬ－２Ｒ水平及Ｔ淋巴细胞亚群，结果显示，急性和慢性丙型肝炎的ＳＩＬ－２Ｒ均较对照组明显升高，且急性肝炎高于慢性肝炎，同时发现ＳＩＬ－２Ｒ水平与肝脏损害程度呈正相关。急性与慢性肝炎的ＣＤ３、ＣＤ４、ＣＤ４／ＣＤ８比值较对照组降低，尤以慢性肝炎降低明显。对３３例ＨＣＶ－ＲＮＡ（＋）的丙型肝炎患者应用干扰素治疗，结果显示，治疗后血清ＳＩＬ－２Ｒ水平低及ＣＤ４／ＣＤ８比值     

Graves病血清可溶性白细胞介素—2受体（SIL—2R）的变化及与甲状腺自身抗 …

按临床表现,体征,ＦＴ３、ＦＴ４、ＴＳＨ测定及治疗情况将６３例Ｇｒａｖｅｓ病患者分为未治组与治疗缓解组,用ＥＬＩＳＡ法测定血清ＳＩＬ－２Ｒ,结果显示血清ＳＩＬ－２Ｒ未治组与治疗缓解组相比是显著增高的（Ｐ〈０．０１）,但治疗缓解组与正常对照组无差异,血清ＳＩＬ－２Ｒ在未组与ＦＴ３水平及甲状腺刺激抗体（ＴＳＩ）、甲状腺蛋白抗体（ＴＧ－Ａｂ）滴度呈正相关（ｒ分别为０．６７８、０．５８０、０．７３０、Ｐ均     

囊虫病患者治疗前后血清可溶性白细胞介素2受体水平的观察

本文采用双抗体夹心ELISA法对经阿苯达唑治疗前后囊虫病人血清可溶性白细胞介素2受体（SIL—2R）水平进行了检测。结果表明治疗前（23人）血清SlL一2R含量为382.15±171.5，治疗后（34人）为373.03±147.36，差异不显著（P＞0.05）。证明阿苯达唑对囊虫病人免疫功能低下状态没有明显的改善作用。     

消化性溃疡活动期脾胃湿热证胃液及血前列腺素E2水平的研究

目的 :探讨胃液及血前列腺素 E2 (PGE2 )水平与消化性溃疡活动期脾胃湿热证的关系。方法 :采用放射免疫法对 30例确诊为消化性溃疡活动期 (APU)脾胃湿热型血液及胃液中 PGE2 水平进行检测 ,并与 2 0例非脾胃湿热型和正常对照组比较。结果 :APU患者胃液中 PGE2 低于正常对照组 ,且以脾胃湿热型为最低。而血中 PGE2检测结果刚好与此相反。结论 :PGE2 与 APU脾胃湿热证的形成有一定关系。     

Gastric Ulcer and Risk of Cancer

Abstract In 1976, 121 patients with benign gastric ulcer and 13 with gastric carcinoma were diagnosed in our department by endoscopy, cytology and directed biopsies. At a 5-year follow-up, 78 of these patients were re-examined with endoscopy and biopsies. None had developed gastric cancer during the observation time. Of the 78 patients who underwent endoscopy, 16 had gastric ulcer, 2 duodenal ulcer and 27 atrophic gastritis, including 3 with moderate dysplasia of the gastric mucosa. The patients with ulcer had remarkably few symptoms. Only few data are available concerning the postulated link between gastric ulcer disease and gastric malignancy. The cancer-ex-ulcere hypothesis seems to be a medical dogma. However, well planned prospective studies with endoscopic follow-up of gastric ulcers are needed to elucidate the question properly     

Regulatory T Cells and Myeloid-Derived Suppressor Cells in Patients with Peptic Ulcer and Gastric Cancer

Background: Regulatory T Cells (Tregs) and Myeloid-Derived Suppressor Cells (MDSCs) are two main regulatory cells modulating the immune responses in inflammation and cancer. Objective: To investigate and compare Tregs and MDSCs in peptic ulcer and gastric cancer. Methods: Patients with dyspepsia were selected and divided into three groups of non-ulcer dyspepsia (NUD, n=22), peptic ulcer disease (PUD, n=25), and gastric cancer (GC, n=27) according to their endoscopic and histopathological examinations. Helicobacter pylori infection was diagnosed by rapid urease test and histopathology. The number of peripheral blood CD4+CD25+FoxP3+Tregs and CD14+HLA-DR- MDSCs were determined in all patients, by flow cytometry. The number of FoxP3+ regulatory T cells was also determined by immunohistochemistry (IHC). Results: The percentage of peripheral blood Treg cells in both PUD )0.81 ± 0.39, p<0.001) and GC groups )0.98 ± 0.65, p<0.001) were significantly higher than in NUD group (0.46 ± 0.10). These results were also confirmed by IHC. A significantly higher percentage of MDSCs in patients with PUD )0.73 ± 0.19, p<0.001) and GC )0.73 ± 0.16, p<0.001) was also observed when compared to NUD group )0.46 ± 0.16). There was no difference in the percentages of these two cell types between the PUD and GC groups. The percentages of Tregs and MDSCs in patients with PUD and GC were not significantly correlated. Conclusions: Both Tregs and MDSCs showed higher frequencies in PUD and GC. These results suggest that immune-modulation by the Tregs and MDSCs may play a role in the pathogenesis of PUD and GC.     

Gastric Biopsy in Peptic Ulcer: A Follow-up Study

Fundal biopsy was performed in 155 peptic ulcer patients (GU 65, DU 90) and antral in 43 (GU 17, DU 26) of the 155 patients. In GU fundal gastritis was found in 74 and antral in 94 % of the cases. In DU the percentages were 16 and 65 respectively. In a short-term follow-up of 30 patients (GU 15, DU 15) the state of the fundal macosa remained essentially unchanged in 19, became worse in 6, and possibly showed an improvement in 5. In a 1–6 year follow-up of 58 patients (GU 28, DU 30) the same type of mucosal structure was preserved in 40, worsening of the changes in 13, and some improvement in 5.     

消化性溃疡,胃癌患者胃窦粘膜中胃肠激素含量变化及其意义

研究了胃十二指肠疾病患者胃窦粘膜胃泌素（Ｇａｓ）、生长抑素（ＳＳ）、Ｐ－物质（ＳＰ）的含量变化及其意义。结果表明：胃窦粘膜ＳＳ含量在胃溃疡组低于其余各组（Ｐ均〈０．０５），而在胃癌时则显著增高（Ｐ〈０．００１）；ＳＰ浓度在十二指肠溃疡组显著低于其余各组（Ｐ均〈０．０５）；胃癌患者Ｇａｓ水平显著高于对照组（Ｐ〈０．０５）；ＳＳ与ＳＰ在十二指肠溃时呈明显负相关。提示：胃粘膜中Ｇａｓ、ＳＳ、ＳＰ的含量变     

红藤蒲贝煎治疗消化性溃疡疗效分析

红藤蒲贝煎由《景岳全书》中连翘金贝煎化裁组成，具有清热解毒、活血化瘀、理气止痛、制酸敛疡等作用。应用红藤蒲口煎治疗消化性溃疡54例，同时与用呋硫硝胺治疗消化性溃疡30例进行对照。结果，在改善临床症状，促使幽门螺杆菌根除以及降低溃疡复发率等方面。治疗组疗效均明显优于对照组。     

消溃异功散合雷尼替丁治疗消化性溃疡48例疗效观察

采用消溃异功散合雷尼替丁治疗消化性溃疡48例，并与西药对照组（32例）．比较，结果两组缓解疼痛疗效相近（P＞0．05），溃疡愈合率治疗组为91．6％，对照组65．5％（P＜0．05）；1年复发率治疗组22．9％，对照组81．2％（P＜0．01）；治疗组在缓解其他症状方面亦优于对照组。提示中西医结合疗法是提高消化性溃疡疗效的有效途径之一。     

A Comparison Study of Gastric Cancer Risk in Patients with Duodenal and Gastric Ulcer: Roles of Gastric Mucosal Histology and p53 Codon 72 Polymorphism

Gastric ulcer is positively, and duodenal ulcer negatively, associated with the risk of gastric cancer. The relationship between a common p53 polymorphism at codon 72 and gastric cancer risk in patients with gastric and duodenal ulcer was examined in 397 Caucasian patients using PCR-RFLP. Noncardiac cancer patients had a distribution pattern of codon 72 genotypes similar to that of other non-cancer patient groups, though the frequency of the Pro/Pro genotype looks higher in duodenal ulcer. However, patients with cancer of the cardiac region had a significantly higher frequency of the Arg/Arg genotype than patients with chronic gastritis, duodenal ulcer, and noncardiac cancer. There was no significant difference in the distribution patterns between gastric ulcer and noncardiac or cardiac cancer or between gastric and duodenal ulcer. These findings may be a reflection of differences in the interaction between p53 codon 72 polymorphism and local factors in the stomach.     

溃疡宁治疗和维持治疗消化性溃疡的临床研究

用中药溃疡宁对70例消化性溃疡患者进行治疗及维持治疗，并与雷尼替丁及灭滴灵联用组对照，结果两组经过4～8周的治疗后，溃疡愈合率相似（P＞0．05）；而幽门螺杆菌的清除率治疗组明显优于对照组（P＜0．01）；维持治疗1年内消化性溃疡的复发率治疗组显著低于对照组（P＜0．05）。结果提示溃疡宁是治疗和维持治疗消化性溃疡的有效药物。     

Evaluation of Serum Interleukin-18 levels in Helicobacter Pylori-infected Peptic Ulcer Patients and its Association with Bacterial CagA Virulence Factor

Background: Helicobacter pylori infection is one of the most common gastrointestinal infections worldwide. Predominant T-helper 1 (Th1) responses with increased gamma interferon (IFN- γ) levels have been proposed to play an important role in H. pylori-induced peptic ulcer. However, bacterial factors contributing to the initiation of Th1 polarization of H. pylori-specific immune responses have not been characterized. Objective: Comparing serum concentrations of IL-18 in H. pylori-infected peptic ulcer (PU) patients, H. pylori-infected asymptomatic (AS) carriers and healthy control group and its association with bacterial virulence factor CagA. Methods: Thirty H. pylori-infected PU patients (20 patients were positive for anti-CagA antibody and 10 patients were negative for anti-CagA antibody), 30 H. pylori-infected (AS) carriers (15 subjects with positive test for anti-CagA antibody and 15 subjects with negative test for anti-CagA antibody) and 20 healthy uninfected subjects were included in this study. Serum concentration of IL-18 was measured by ELISA method. Results: The mean serum levels of IL-18 in PU patients (333.2 pg/ml ± 158), was significantly higher than those found in AS (146.5 pg/ml ± 90.1; P<0.001) and healthy control (82.2 pg/ml ± 45.7; P<0.0001). In both PU and AS groups, mean serum IL-18 levels in subjects with positive test for anti-CagA antibody were significantly higher than those observed in subjects with negative test for anti-CagA antibody. No significant difference was observed between serum IL-18 levels of healthy uninfected control and AS carriers with negative test for anti-CagA antibody. Conclusion: The results of the present study showed higher serum concentrations of IL-18 in peptic ulcer patients compared with H.Pylori carriers and healthy controls. This difference in cytokine levels may be explained by differential expression of H.Pylori CagA gene during the course of the infection.