异鼠李素-3,7-二-O-β-D-葡萄糖苷的保肝降酶试验

目的：验证异鼠李素－3，7－二－O－β-D-葡萄糖苷的保肝降酶作用，考察其作为垂盆草药材质控指标的可行性。方法：用垂盆草药材n-BuOH部分经过溶剂分离和色谱分离制得的异鼠李素－3，7-二－O－β-D－葡萄糖苷，配成一定浓度的溶液，通过保肝降酶试验（D－氨基半乳糖致小鼠急性肝损伤）,考察其活性。结果：和模型组比较，该成分具有一定的保肝降酶活性，高剂量组更加明显。结论：异鼠李素－3，7－二－O－β-D-葡萄糖苷作为垂盆草药材的质控指标是可行的。     

灵猫方对急性肝损伤模型保肝降酶作用的实验研究

应用急性肝损伤动物模型观察灵猫方的保肝降酶作用。方法：建立ANIT小鼠急性肝损伤、CCl4大鼠急性肝损伤动物模型,实验分为正常对照组、模型组、阳性对照组、灵猫方低剂量组、灵猫方中剂量组和灵猫方高剂量组,分别给予相应处理,处死动物。分离血清测定AST、ALT。结果：与模型组相比,灵猫方低剂量、中剂量和高剂量可明显降低ANIT急性肝损伤小鼠ALT水平（P〈0．05）,灵猫方中剂量、高剂量组的AST均明显降低（P〈0．05）;与模型组相比,灵猫方低剂量、中剂量和高剂量可明显降低CCI。急性肝损伤大鼠的ALT水平（P〈0．05）,低剂量组的AST水平明显降低（P〈0．05）。结论：灵猫方对急性肝损伤具有保肝降酶作用。     

鼻脑通鼻用喷雾剂抑制过氧化氢致SH SY5Y神经细胞损伤的研究

目的：研究鼻脑通对H2O2诱导的人神经母细胞瘤细胞系SH SY5Y细胞损伤的影响。方法：培养的人神经母细胞瘤细胞系SH SY5Y细胞给予鼻脑通（004,04,4 μg·mL-1）预孵育24 h后,加入H2O2 1 000 μmol·L-1作用18 h诱导产生损伤,测定细胞上清液中血管活性肽内皮素 1（ET 1）、降钙素基因相关肽（CGRP）;炎症因子白细胞介素 1α（IL 1α）、白细胞介素 8（IL 8）;致痛物质P物质（SP）、5 羟色胺（5 HT）、一氧化氮（NO）的含量。结果：与对照组相比,损伤组细胞上清液血管活性肽ET 1、CGRP含量显著增多（P＜001,P＜005）;炎症因子IL 8含量显著增多（P＜001）;致痛物质SP、5 HT、NO含量显著增多（P＜001,P<001,P＜005）。而与损伤组相比,鼻脑通中、高剂量组细胞上清液血管活性肽ET 1含量降低（P＜005,P＜0001）,且炎症因子IL 8含量也降低（P＜005,P＜001）;鼻脑通中、高剂量组致痛物质SP含量比损伤组减少（P＜005,P＜005）,高剂量组5 HT、NO含量比损伤组减少（P＜005,P＜005）。结论：鼻脑通治疗偏头痛的机制可能与降低H2O2诱导的SH SY5Y细胞损伤后血管活性肽ET 1、炎症因子IL 8释放以及抑制致痛物质SP、5 HT、NO的释放有关。     

广西桂郁金对肝纤维化大鼠血清学指标的影响

目的：观察广西桂郁金（GGYJ）对肝纤维化（HF）大鼠的抗肝损伤、调节蛋白质代谢、抗HF作用。方法：采用皮下注射四氯化碳（CCl4,）油溶液造成HF模型,以秋水仙碱为阳性对照,观察GGYJ对血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、总蛋白（TP）、白蛋白（ALB）、球蛋白（GLB）、透明质酸（HA）、层粘连蛋白（LN）、HI型前胶原（PcHI）含量的影响。结果：与模型组比较,GGYJ高、中剂量组能够明显抑制ALT、AST水平的升高（P〈0．01）;GGYJ低剂量组降低ALT、AST水平的作用不明显。GGYJ高、中、低剂量组都能提高ALB的水平（P〈0．01）,降低GLB的水平（P〈0．01）,增高A／G比值（P〈0．01或P〈0．05）。GGYJ高剂量组能显著降低HA、LN、PCⅢ的含量（P〈0．01或P〈0．05）。GGYJ中、低剂量组能显著降低HA、LN含量（P〈0．01或P〈O．05）,但对PCⅢ的含量无明显影响。其中GGYJ高剂量组降低ALT、AST水平,提高ALB水平的作用与秋水仙碱组相当。结论：GGYJ有保肝降酶、改善蛋白质合成、抗HF作用。     

垂盆草总黄酮的保肝降酶作用及其化学成分的鉴别研究

目的 进一步验证垂盆草总黄酮的保肝降酶作用,鉴别并确证垂盆草总黄酮的化学组成,为垂盆草药材质量控制指标的确认和质量标准的研究奠定基础.方法 通过CCl4造模,考察垂盆草总黄酮对小鼠血清转氨酶的影响及对肝脏的保护作用.采用HPLC法,通过与各对照品对照,确证垂盆草总黄酮的化学组成.结果 垂盆草总黄酮能显著降低小鼠血清ALT和AST,并对肝脏有明显的保护作用;垂盆草总黄酮中至少含有槲皮素、山柰素、木犀草素等8种黄酮类化合物.结论 垂盆草总黄酮是垂盆草中重要的保肝降酶活性部位之一,垂盆草总黄酮中至少含有槲皮素、山柰素、木犀草素等8种黄酮类化合物.这为确认垂盆草药材的质量控制指标以及制定科学的质量标准奠定了基础.     

大黄酚对脑缺血再灌注小鼠心脏组织过氧化氢及过氧化氢酶的影响

目的：研究大黄酚（ chrysophanol，Chry）对脑缺血再灌注（ IR）后小鼠心脏组织中过氧化氢（ H2 O2）及过氧化氢酶（ CAT）的影响，探讨大黄酚对脑缺血再灌注后对心脏损伤的保护作用机制。方法取昆明种小鼠80只，3．5％水合氯醛麻醉后，制备小鼠IR模型。将造模成功的74只小鼠随机分为5组，药物组（IR＋Chry）45只，分为高、中、低剂量3个组，各15只，模型组15只，假手术组14只。药物组于缺血前30 min分别腹腔注射大黄酚10．0、1．0、0．1 mg/kg；模型组于缺血前30 min腹腔注射溶剂10 mL/kg；假手术组只进行手术，但不进行IR，腹腔注射溶剂10 mL/kg。模型组和药物组小鼠恢复血供后10 min快速断头处死，取心脏组织，制成10％心脏组织匀浆，测定小鼠心脏组织中H2 O2含量和CAT活力。结果与假手术组比较模型组小鼠心脏组织内H2O2含量明显增加﹙P＜0．05），心脏组织内CAT活力明显降低﹙P＜0．05）；与模型组比较高剂量大黄酚组、中剂量大黄酚组小鼠心脏组织内H2 O2含量明显减少（ P＜0．01）。与模型组比较高剂量大黄酚组小鼠心脏组织内CAT活力明显提高（ P＜0．01）。结论大黄酚对脑IR心脏损伤的保护作用可能通过提高心脏组织中CAT活力起作用。     

凉血解毒法为主配合激素治疗慢性乙型肝炎自身抗体阳性的临床研究

目的：探讨凉血解毒法为主配合激素治疗慢性乙型肝炎（CHB）自身抗体阳性的临床疗效及可能的作用机制。方法：91例患者分为中西医结合组30例,西药组29例,基础组32例。基础组给予常规保肝降酶治疗,西药组在保肝降酶的基础上增加生理剂量激素治疗,中西医结合组在保肝降酶和加用生理剂量激素的基础上,采用凉血解毒中药治疗。结果：3组患者治疗后纳减、腹胀、肝区不适、乏力等症状均有不同程度缓解,3组间比较,差异无显著性意义（P〉0．05）。中西医结合组及基础组治疗后尿黄例数减少,西药组则增多,前两者与后者比较,差异有显著性意义（P〈0．05）。西药组有11例、中西医结合组有3例出现面部痤疮或原有痤疮加重,基础组则无变化,中西医结合组、基础组与西药组比较,差异有显著性意义（P〈0．05）。3组治疗后,ALT、AST均较治疗前显著下降,与治疗前比较,差异有显著性意义（P〈0．05）。3组治疗后LN（层粘连蛋白）、PCⅢ（Ⅲ前胶原）、Ⅳ—C（Ⅳ胶原）3项肝纤维指标均有不同程度下降,但3组间比较,差异无显著性意义（P〉0．05）。HA（透明质酸）中西医结合组治疗后下降最为明显,与西药组和基础组比较,差异有显著性意义（P〈0．05）。治疗后5项自身抗体总阳性率、BLys（B淋巴细胞刺激因子）OD值中西医结合组和西药组均低于基础组,差异有显著性意义（P〈0．05）。结论：凉血解毒法为主与生理剂量激素联合能有效降低自身抗体阳性CHB患者BLys水平,抑制自身抗体的产生,促进肝功能更快恢复。     

没食子酸丙酯对H2O2诱导SH-SY5Y细胞氧化损伤的影响及其作用机制

目的 探讨没食子酸丙酯（propyl gallate,PG,赤芍801）对H2O2诱导人神经母细胞瘤细胞（SH-SY5Y）氧化损伤的影响及其作用机制。方法 将SH-SY5Y细胞均分为对照组、H2O2模型组、PG1组、PG2组和PG3组,对照组常规加入DMEM/F12培养液培养;H2O2模型组加入H2O2 200 μmol·L-1;PG1组加入PG 20 μmol·L-1+H2O2 200 μmol·L-1;PG2组加入PG 40 μmol·L-1+H2O2 200 μmol·L-1;PG3组加入PG 80 μmol·L-1+H2O2 200 μmol·L-1。比较5组SH-SY5Y细胞的增殖活性,细胞培养物上清中8-OHdG,细胞内ROS、LDH释放量,Hoechst 33258染色结果、细胞周期变化情况及Caspase-3酶活性。结果 H2O2模型组细胞存活率显著低于对照组,细胞培养物上清中8-OHdG水平显著高于对照组,ROS水平显著高于对照组,LDH释放量显著高于对照组,具有非常显著性差异（P＜0.01）;PG1组、PG2组和PG3组细胞存活率均显著高于H2O2模型组,细胞培养物上清中8-OHdG水平均显著低于H2O2模型组,ROS水平均显著低于H2O2模型组,LDH释放量均显著低于H2O2模型组,差异具有统计学意义（P＜0.05）;PG可显著改善SH-SY5Y细胞凋亡情况,且具有剂量依赖关系;H2O2模型组G0/G1期百分率显著高于对照组,S期、G2/M期和PI均显著低于对照组,差异具有统计学意义（P＜0.05）;PG2组、PG3组G0/G1期百分率显著低于H2O2模型组,S期、G2/M期和PI均显著高于H2O2模型组,差异具有统计学意义（P＜0.05）;H2O2模型组Caspase-3酶活性显著高于对照组,差异有统计学意义（P＜0.05）;PG2组和PG3组Caspase-3酶活性显著低于H2O2模型组,差异有统计学意义（P＜0.05）。结论 PG在一定剂量范围内对H2O2诱导的SH-SYSY神经细胞损伤具有明显的保护作用,其保护效应可能与清除ROS,减轻DNA氧化损伤,抑制线粒体通路介导的细胞凋亡有关。     

甘宁颗粒对慢性乙型病毒性肝炎患者血清IFN—γ影响的研究

目的：观察甘宁颗粒治疗慢性乙型病毒性肝炎的临床疗效及其对慢性乙型病毒性肝炎患者血清IFN—γ水平的影响。方法：将60例慢性乙型病毒性肝炎患者随机分为治疗组与对照组各30例,2组均采用保肝降酶治疗,治疗组同时予甘宁颗粒,对照组只给予保肝降酶治疗,对2组患者治疗前后的临床症状、血清肝脏生化功能ALT、AST,血清病毒学标志HBVDNA和血清IFN—γ水平进行比较、分析及评价。结果：甘宁颗粒改善慢性乙型病毒性肝炎患者的临床症状,治疗6个月后临床症状的总有效率为76．7％,与对照组比较有统计学意义（P〈0．05）。甘宁颗粒治疗后血清IFN—γ水平明显升高（P〈0．01）,血清肝脏生化功能ALT、AST指标明显好转（P〈0．01）,血清病毒学标志HBV DNA的滴度下降（P〈0．05）。与对照组比较有统计学意义（P〈0．05）。临床试验中2组均未见不良反应。结论：甘宁颗粒治疗慢性乙型病毒性肝炎疗效肯定,能显著改善慢性乙型病毒性肝炎患者的临床症状,提高患者血清IFN—γ水平。     

蒙药沙棘-10味散对CCl4诱发的急性肝损害的保护作用及急性毒性试验的研究

目的：研究蒙药沙棘-10味散降酶保肝作用及其急性毒性作用。方法：将蒙药沙棘-10味散分为高、中、低刺量组和护肝片组分别灌胃7天，腹腔注射CCl4引起小鼠急性肝损害，16h后取血．分离血清．检测血清ALT、AST活性；取肝组织，观察肝脏形态学的变化。采用近似LD50试验方法，24h大剂量、多次连续灌胃给药60g/kg体重，测出该药LD50，或最大耐受剂量，并检测血清ALT、AST含量，观察肝组织形态学的改变。结果：模型组与正常组比较，小鼠血清AST、ALF显著升高（P〈0．01）：沙棘-10味散高、中、低剂量组和护肝片组与模型组比较均有显著降低血清AST、AIT含量作用（P〈0．05）：而沙棘-10味散高、中、低剂量组降低血清AST、ALT含量显著优于护肝片组（P〈0．05）。模型组肝组织学改变主要表现为碎片状坏死；护肝片组可见散在点状及小碎片状坏死；沙棘-10味散高、中、低剂组肝组织损伤主要表现为细胞水肿、脂肪变性，偶有小点状坏死。急性毒性试验未观察到明显毒性反应，无动物死亡，测得最大耐受剂量MTD〉60g／kg。相当于成人日用药量的1200倍以上。结论：沙棘-10味散和护肝片均有降酶保肝作用，而且沙棘-10味散作用更显著。沙棘-10昧散大剂量服用比较安全．     

The anti-inflammatory and hepatoprotective effects of fractions from Cudrania cochinchinensis var. gerontogea.

Various fractions of the ethanol extract from the root wood of Cudrania cochinchinensis var. gerontogea (Moraceae) were evaluated for their anti-inflammatory effects on carrageenan-induced edema and hepatoprotective activities on carbon tetrachloride (CCl4)-induced and D-galactosamine-(D-GalN) induced acute hepatotoxicity in rats. The fractions (n-hexane, CHCl3, EtOAc, n-BuOH, and H2O) displayed significant inhibitory activity against carrageenan-induced edema, and the active anti-inflammatory components were further localized in the n-BuOH fraction, which exhibited the greatest anti-inflammatory effect, an effect 5% greater than indomethacin (which was used as a standard reference substance). Each fraction exerted a significant hepatoprotective effect by reducing enzymatic alteration (sGOT and sGPT) and by improving hepatic lesions, including liver centrilobular inflammation, cell necrosis, fatty change, ballooning degeneration in CCl4-induced acute hepatitis; and necrosis of the portal area in D-GalN-induced acute liver injury. The n-BuOH and EtOAc fractions had the greatest hepatoprotective effects on CCl4-induced liver injury; in contrast, the CHCl3 fraction was most potent against D-GalN intoxication, which is comparable to silymarin, as a recognized hepatoprotective drug.     

滇越金线兰对高脂饮食联合STZ诱导的大鼠血糖、血脂和肾功能的影响

目的:研究滇越金线兰等剂量的总膏及其各极性部位对高脂饮食联合STZ诱导的大鼠模型血糖、血脂和肾功能的影响。方法:对滇越金线兰的总膏用不同极性的溶剂萃取,得到石油醚部位、乙酸乙酯部位、正丁醇部位和水部位。测定总膏和各极性部位对高脂饮食联合STZ诱导的模型大鼠给药后血浆的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、肌酐(CRE)和尿素氮(BUN)。肾脏切片,进行病理学检查。结果:滇越金线兰的乙酸乙酯部位(4 g/kg)可以显著降低模型大鼠的血糖、改善血脂代谢,减少肌酐和尿素氮。各给药部位对糖尿病并发的早期肾损害有改善作用。结论:乙酸乙酯部位为滇越金线兰降血糖、降血脂和改善糖尿病肾病并发症的主要活性部位。     

Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of Sedum sarmentosum extract

This study aimed to assess some novel pharmacological activities of Sedum sarmentosum Bunge, a perennial herb widely distributed on the mountain slopes in Oriental countries and traditionally used for the treatment of certain inflammatory diseases. Sedum sarmentosum was extracted with absolute methanol to generate the methanol extract (SS). SS exhibited a significant inhibitory activity in the chick embryo chorioallantoic membrane (CAM) angiogenesis in a dose-dependent manner (IC(50)=2.29 microg/egg). The anti-nociceptive activity of SS was demonstrated using acetic acid-induced writhing model in mice. SS reduced the levels of anti-inflammatory markers, such as volume of exudates, number of polymorphonuclear leukocytes and nitrite content, in the air pouch model. It dose-dependently exhibited an inhibitory activity in the acetic acid-induced vascular permeability in mice. It suppressed production of nitric oxide in the lipopolysaccharide (LPS)-activated RAW264.7 macrophages. Additionally, it suppressed induction of inducible nitric oxide synthase (iNOS) in the activated macrophages. In brief, the results provide some pharmacological basis for the therapeutic use of Sedum sarmentosum.     

Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats

The hepatoprotective effect of ethanolic extract and its four different fractions (CHCl(3), EtOAc, n-BuOH, and remaining water fraction) of Vitis vinifera L. leaves was investigated against carbon tetrachloride (CCl(4))-induced acute hepatotoxicity in rats. The ethanolic extract was found active at 125mg/kg dose (per os). The ethanolic extract was fractionated through successive solvent-solvent extractions and the n-BuOH fraction in 83mg/kg dose possessed remarkable antioxidant and hepatoprotective activities. Liver damage was assessed by using biochemical parameters (plasma and liver tissue MDA [malondialdehyde], transaminase enzyme levels in plasma [AST-aspartate transaminase, ALT-alanine transferase] and liver GSH [glutathione] levels). Additionally, the pathological changes in liver were evaluated by histopathological studies. Legalon 70 Protect was used as standard natural originated drug.     

垂盆草总黄酮对APAP诱导小鼠肝损伤的保护作用

目的 研究垂盆草总黄酮对对乙酰氨基酚(acetaminophen,APAP)所致小鼠肝损伤的保护作用及其可能的作用机制.方法 将48只ICR雄性小鼠随机分为6组(n= 8),正常对照组,模型组,阳性药物组(联苯双酯,300 mg/kg),垂盆草总黄酮低(150 mg/kg)、中(300 mg/kg)、高剂量组(600 ...     

凤尾草中倍半萜类成分研究

目的研究江西产凤尾草的化学成分,寻找活性成分。方法凤尾草经70%乙醇提取再经石油醚、乙酸乙酯和正丁醇依次萃取,乙酸乙酯及水部位用硅胶、Sephadex LH-20等柱色谱进行分离纯化,通过1H-NMR、13C-NMR、MS等方法鉴定化合物的结构。结果从凤尾草中分离鉴定了6个蕨素类化合物,其中4个从乙酸乙酯部位中分离得到,分别为蕨素B(1),乙酰蕨素B(2),去羟基蕨素B(3),蕨素Z(4);2个从水部位中分离得到,分别为蕨素C 3-O-β-D-葡萄糖苷(5),蕨素Q 3-O-β-D-葡萄糖苷(6)。结论化合物2,3,4及6为首次从该植物中分离得到。     

In vitro GABA-transaminase inhibitory compounds from the root of Angelica dahurica

The 80% aqueous MeOH extracts from the root of Angelica dahurica, found to inhibit the activities of GABA degradative enzymes GABA transaminase (GABA-T) and succinic semialdehyde dehydrogenase (SSADH), were fractionated using EtOAc, n-BuOH and H2O. Repeated column chromatography for the EtOAc and n-BuOH fractions led to the isolation of two new coumarins, oxypeucedanin hydrate-3'-butyl ether and isopraeroside IV along with six known coumarins, isoimperatorin, imperatorin, phellopterin, oxypeucedanin hydrate, nodakenin and 3'-hydroxymarmesinin, and two polyacetylenes, falcarindiol and octadeca-1,9-dien-4,6-diyn-3,8,18-triol. Of the isolated pure compounds, imperatorin and falcarindiol inactivated the GABA-T activities in both time- and concentration-dependent manners. The kinetic studies showed that imperatorin and falcarindiol reacted with the GABA-T with a second-order rate constant of 2.3 +/- 0.2 mm(-1) min(-1) and 1.5 +/- 0.1 mm(-1) min(-1), respectively. It is postulated that imperatorin and falcarindiol are able to elevate the neurotransmitter GABA levels in the central nervous system by an inhibitory action on the GABA degradative enzyme GABA-T.     

Active components from Artemisia iwayomogi displaying ONOO(-) scavenging activity

Peroxynitrite (ONOO(-)) is one of cytotoxic species produced by the reaction between superoxide (*O(2) (-)) and nitric oxide (NO). The main aim of this study was to identify ONOO(-) scavenging constituents from herbs. Methanolic extracts derived from one hundred thirty eight herbs were screened out for their ONOO(-) scavenging activities. Twenty herbs showed excellent ONOO(-) scavenging activity in a dose dependent manner. Of them, Artemisia iwayomogi, which showed the highest activity, was fractioned with several solvents. The ONOO(-) scavenging activity of fractions was in order of ethyl acetate (EtOAc) > n-butanol (BuOH) > dichloromethane (CH(2)Cl(2)) > water (H(2)O) fraction. The EtOAc and the n-BuOH fractions were further purified to isolate active principles by column chromatography. Apigenin 7-methylether (genkwanin), apigenin 7,4'-di-O methylether, jaceosidin, and scopoletin from the EtOAc fraction and chlorogenic acid, 2,4-dihydroxy 6-methoxy acetophenone 4-O-beta-D-glucoside, quebrachitol, and scopolin from the n-BuOH fraction were identified. The scavenging activities of chlorogenic acid (IC(50) = 0.52 +/- 0.04 microM), genkwanin (IC(50) = 1.01 +/- 0.10 microM), and scopoletin (IC(50) = 1.03 +/- 0.15 micro M) were higher than or comparable to a well-known ONOO(-) scavenger, penicillamine (IC(50) = 1.76 +/- 0.18 microM), suggesting that those compounds might be developed as effective ONOO(-) scavengers for, prevention of the ONOO(-)-involved diseases.     

橘叶提取物对小鼠自身免疫性睾丸炎的影响

目的研究橘叶提取物对实验性自身免疫性睾丸炎(EAO)模型小鼠睾丸组织形态及血清白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)含量的影响。方法采用单侧睾丸注射冰醋酸法建立小鼠EAO模型,随机分为空白组、模型组、橘叶乙醇提取物组、环己烷组、乙酸乙酯组、正丁醇组和水部位组。光镜下观察睾丸组织结构变化,ELISA法检测血清IL-1β和TNF-α浓度。结果给药3周后,橘叶乙醇提取物、正丁醇部位和水部位组能明显改善睾丸组织病变,减轻炎症反应。橘叶给药组除环己烷部位外,其余各组均能明显降低EAO小鼠血清中炎症因子IL-1β水平,与模型组比较,差异有统计学意义(P <0. 01)。此外,乙醇提取物组、正丁醇部位组中TNF-α含量也明显降低(P <0. 05,P <0. 01)。结论橘叶乙醇提取物、乙酸乙酯、正丁醇及水部位对小鼠EAO的发生均具有拮抗作用,其中,正丁醇部位抗EAO活性较明显,这可能与抑制炎性因子IL-1β和TNF-α释放有关。     

椭圆叶花锚的化学成分研究

目的:对龙胆科Gentianaceae花锚属植物椭圆叶花锚Halenia elliptica的干燥全草进行化学成分研究.方法:椭圆叶花锚的干燥全草用90%乙醇提取,依次用石油醚、乙酸乙酯、正丁醇萃取,对乙酸乙酯萃取部分采用各种柱色谱进行分离纯化,通过波谱数据分析(1H-NMR,13C-NMR,MS)进行结构鉴定.结果:从乙酸乙酯萃取部分分离鉴定了12个化合物,其中5个(口山)酮,1个裂环烯醚萜苷,以及6个其他类化合物.分别鉴定为8-羟基-2-甲基色原酮(8-hydroxy-2-methylchromone,1),5-羟基-2-甲基色原酮(5-methoxy-2-methylchromone,2),7-表断马钱子苷半缩醛内酯(7-epi-vogeloside,3),松柏醛(coniferl aldehyde,4),芥子醛(sinapaldehyde,5),norbellidifolin(6),1-羟基-2,3,4,6-四甲氧基(口山)酮(1-hydroxyl-2,3,4,6-tetramethoxyxanthone,7),1-羟基-2,3,4,7-四甲氧基(口山)酮(1-hydroxyl-2,3,4,7-tetramethoxyxanthone,8),1-羟基-2,3,5-三甲氧基(口山)酮(1-hydroxyl-2,3,5-trimethoxyxanthone,9),壬二酸(azelaic acid,10),β-谷甾醇(β-sitosterol,11),齐墩果酸(oleanolic acid,12).结论:其中化合物1,2为新的天然产物,化合物3～6,10为首次从椭圆叶花锚中分离得到,化合物6显示较好的抗HBsAg和HBeAg活性,其IC50分别为0.77 mmol·L-1和＜0.62 mmol·L-1.