正常O型人血清ABO血型抗体主要类别的分析

目的通过检测O型及A/B型人血清中IgM类及IgG类抗体的效价,分析正常O型人血清中ABO血型抗体的主要类别。方法分别以盐水介质法和间接抗人球蛋白法检测O型及A/B型人血清中IgM类和IgG类抗A或抗B抗体的效价。结果 O型人血清中IgM类抗A/抗B抗体效价均显著高于IgG类抗A/抗B抗体（均P〈0.01）;O型人血清中IgM类抗A/抗B抗体效价与B/A型人比较无显著性差异（均P〉0.05）,而IgG类抗A/抗B抗体效价显著高于B/A型人（均P〈0.01）;O型人血清中IgG类与IgM类抗A/抗B抗体效价的比值均显著高于B/A型人（均P〈0.01）。结论 O型人ABO血型抗体仍以IgM类为主,但其IgG类抗体效价及在血清中所占的比例要明显高于A/B型人。     

健康人群中IgG类血型抗体高低效价间的肠道微生物差异

目的:探讨影响健康人群IgG类抗体效价水平的关键菌群.方法:2020年1月至2020年2月招募43例复旦大学在校学生为健康志愿者,收集静脉血和新鲜粪便样本.采用抗人球蛋白凝胶卡测定IgG类血型抗体效价,以抗体效价1∶128为临界点分为IgG类抗A抗体效价低水平组(OALG,≤128)、IgG类抗A抗体效价高水平组(OA...     

保山市72928例献血员ABO、Rh血型分布调查

目的了解保山市献血员中主要民族的ABO、Rh血型分布,建立本地稀有血型献血员档案,满足无偿献血员的招募和临床用血需要。方法用微板法对ABO、Rh血型进行检测。抽取124名RhD阴性者,用试管法对C/c、E/e进行筛选。结果 7个主要献血民族中,汉、彝、白、傈僳、回族ABO血型分布为A>O>B>AB;布朗、傣族为O>B>A>AB;Rh阴性比率:汉族0.33%,彝族0.35%,白族0.09%,布朗族0.00%,傈僳族0.32%,傣族0.21%,回族0.18%;Rh表型主要有ccdee(60.5%)、Ccdee(33.1%)、CCdee(4.0%)、ccdEe(2.4%),没有发现其他表型。结论保山市汉、彝、白、傈僳、回族献血员ABO血型分布率与中国汉族人群的分布相符;布朗、傣族则与其不同;Rh阴性频率处于0.00%~0.35%之间,体现了血型分布在本市的地域和民族特点。     

Genotyping of samples lacking expected antibodies in ABO blood group

We report nine donations with ABO inconsistency in reverse typing caused by partly or entirely missing antibodies. A and B antigens and antibodies were examined by serological blood typing, and ABO deoxyribonucleic acid (DNA) analyses were performed by sequence-specific priming and sequencing. A B101 allele was demonstrable in a case with O phenotype. The molecular mechanisms in deficiency of natural ABO antibody could be partly clarified. The ABO genotyping technique is an accurate method for determining the blood samples involved in ABO grouping discrepancies and is a valuable complement to serology for correct determination of donor blood status. The mechanisms involved in the absence of potent natural antibodies directed against A and B antigen lacking on an individual's own red cell membranes remain to be further investigated.     

18月龄内婴幼儿ABO血型抗体发育分析

摘要：目的?观察18月龄内婴幼儿ABO血型IgM抗体效价，了解血型抗体发育趋势。方法?收集南京地区18月龄内住院婴幼儿标本，微柱凝胶法检测血型，共获得非AB型标本1 250例，其中A型454例、B型410例、O型386例，利用盐水试管法进一步检测血浆中IgM 血型抗体效价并统计凝集度积分。结果?30日龄内抗A和抗B效价为0的婴幼儿数分别占该年龄组人数的33.88%和47.46%；2～6月间，效价为0及低效价的婴幼儿数逐渐减少；7月龄时，婴幼儿抗A抗体效价均≥2；13月龄时，婴幼儿抗B抗体效价均≥2。凝集度积分结果提示随月龄增长，ABO血型IgM抗体强度逐渐增加。结论?婴幼儿血型IgM 抗A在7月龄时发育基本完成，IgM 抗B在13月龄时发育基本完成，且IgM血型抗体发育有提早趋势。     

Characterization of the humoral immune response in two paediatric patients transplanted with split livers from ABO-incompatible living-related donors: appearance of cytomegalovirus-induced ABO antibodies

Two blood group O paediatric patients, 12 and 6 months old, were transplanted with liver segments from their blood group A2Le (a(-)b+) Se and blood group A1Le (a(-)b+) Se fathers, respectively. Recipient anti-A antibody titres were reduced prior to transplantation by blood exchange. Both patients had rejection episodes in the post-transplant period that were reversed by anti-rejection therapy. No anti-A antibody titre rise occurred concomitant with these rejections. Postoperatively both patients had cytomegalovirus (CMV) infections, and simultaneous with these infections, a strong increase in anti-A antibody titres was seen, but no rejection occurred. The anti-A antibody titre increase seemed to be specific for A antigens, because the anti-B and anti-alphaGal (anti-pig) antibody titres did not show any changes. CMV infection is a serious cause of morbidity and mortality in immunosuppressed patients, and the virus can influence glycosylation of infected cells. Whether this can explain the importance of the infection in relation to the increase in titre remains to be elucidated.     

High titers of anti-A1 and anti-B antibodies among Peruvian group O platelet donors

Critical antibody titers have been described as factors associated with hemolysis in ABO plasma-incompatible platelet (PLT) transfusions. This study was carried out to describe the frequency of high-titers anti-A and antiB IgM and IgG antibodies in group O apheresis platelet donors, and to explore differences according to the donor characteristics. A cross-sectional study was carried out at the Blood Bank of a National Hospital in Peru from January to March 2019. IgM and IgG antibodies against A1 and B antigens were quantified in 339 platelet donors using the direct hemagglutination technique and the solid-phase adherence technique, respectively. For analysis purposes, two cut-off points; ≥128 and ≥64, were used to define a critical titer for IgM due to a lack of consensus. An IgG titer of ≥256 was also defined as critical. Of the donors, 22.1 % had critical IgM titers when the cut-off point was defined as ≥128. However, when the IgM cut-off was ≥64, the frequency of platelet donors with critical titers increased to 54.0 %. The frequency of donors with critical IgG titers was 23.5 %. Higher IgG titers were associated with female donors while higher IgM titers were negative associated with age. One in two or three platelet donors, depending on the cutoff point used to define a critical IgM titer, had at least one critical titer of anti-A or anti-B antibodies. Early identification of platelet donors with critical antibody titers could prevent passive transfusion of ABO antibodies to non-isogroup recipients.     

国产二甲基二丁基氯化胺试剂替代凝聚胺检测IgG类血型抗体的初步应用

利用国内自行合成的聚胺阳离子 (Polycation)材料二甲基二丁基氯化胺及数种廉价辅助材料成功地建立了代替进口凝聚胺 (polybrene)技术检测IgG类血型抗体的PDC三试剂法。本法不仅比传统方法操作简便、作用时间短、灵敏度高 ,且达到了进口凝聚胺的技术水平。本法原理同凝聚胺技术 ,可用于血型鉴定 ,交叉配血、抗体筛选。另外 ,对弱的IgM类血型抗体的反应也有明显的增强作用。     

8860名学生ABO血型分布及批量检测ABO血型错检原因分析

[目的]了解学生群体中的ABO血型分布情况,有利于临床医务人员及相关工作人员掌握血源概况,为血液血型贮备对策提供科学依据,并分析造成批量ABO血型错检的原因。[方法]运用玻片凝集法和试管离心法对8860名学生进行ABO血型鉴定,对可疑血液样本做盐水试管法的正反定型试验。[结果]血型分布为A型占27．75％,B型占31．65％,O型占29．65％,AB型占10．95％。[结论]8860名学生血型分布为B〉O〉A〉AB。当发现血型鉴定出现可疑时,首先应重复做试验一次,严格执行操作规程,细心观察结果,按步骤一步步排除错检的可能性。     

ABO antibody titer performance characteristics and correlates between two automated platforms

BackgroundAABB standards require a policy for assessing transfusing ABO-incompatible plasma. After a fatal hemolytic event with incompatible plasma, our institution instituted platelet donor population titer method for ABO antibodies on the PK7300, with high-titer being defined as having isohemagglutinin titers greater than 256. We recently switched titering platforms to the Neo Iris and we seek to determine the equivalent isohemagglutinin high-titer cutoff on the Neo Iris as compared to the PK7300.MethodsWe measured the titers on 299 apheresis platelet donors and compared its performance characteristics at various cutoffs to the PK7300 reference standard. Discrepant results were manually diluted and retested on the Neo Galileo. Furthermore, since the Neo Iris is able to determine isotype and antigen specific titers, we also characterized these features in our donor population.ResultsIgM titer of 128 on the Neo Iris has better accuracy compared to the titer of 64 (94 % vs 93.6 %). Eleven of sixteen discordant results were in agreement with Neo Iris. Blood group O had the highest IgG antibody titers for both anti-A and anti-B (p = 8.4E-17 and 4.3E-09, respectively). Additionally, group O donors exhibited lower anti-A2 than anti-A1 IgG titers.DiscussionThe Neo Iris titer cut-off of 128 had the best overall accuracy and correlation with a 256 cut-off on our laboratory developed test on the PK7300 platform. Additionally, we found that group O donors had the highest titer antibodies, with typically higher IgG titers than IgM, and generally multiple dilution levels greater than other blood types.     

4560例孕妇ABO血型抗体检测回顾性分析

目的 研究O型孕妇ABO血型抗体效价异常在孕妇中所占比率及临床意义。方法利用凝胶微柱技术进行IgG抗A或抗B的ABO血型抗体效价检测。结果4560例孕妇中,血清效价≥1：64者851例,异常检出率为18．6％。结论O型孕妇中ABO血型抗体效价异常所占比率较高,因此应及时检测孕妇的ABO血型抗体,便于早发现异常,及时治疗,以减少新生儿溶血病的发生,达到优生优育的目的。     

去除血型抗体制备O型红细胞的研究

目的建立去除血浆中血型抗体的O型红细胞的制备工艺,为临床提供安全的O型红细胞制剂。方法使用处理后的冰冻干燥A型、B型红细胞膜,分别加入O型红细胞血浆中吸附血浆血型抗体物质,并检测吸附后红细胞的结构和功能。结果使用海藻糖冻干保存红细胞膜可保存其抗原性,吸附后,可去除血浆中抗A、抗B抗体80%—99%,同时红细胞变形指数、ATP、渗透脆性、上清游离Hb含量与吸附前(正常红细胞)比较差异无统计学意义(P>0.05),均在正常参考范围;流式结果显示,吸附后采用离心方法,红细胞膜去除率可达到77%。结论用冰冻干燥红细胞膜去除ABO血型抗体为O型红细胞的他型输注奠定了基础。     

母子ABO血型不合的产前诊断

目的探讨微柱凝集技术在诊断母子ABO血型不合的产前应用。方法采用BioVue系统微柱凝集技术，对200例丈夫血型为非O型的O型血孕妇检测血清IgG抗体效价。结果产后婴儿母子ABO血型不合新生儿溶血性疾病（HDN）发病率随母亲血清IgG抗体效价增高而上升。结论BioVue系统微柱凝集技术能准确检测孕妇血清IgG抗体效价，是产前预报因母子ABO血型不合而引起HDN的可靠方法。     

母子ABO血型不合的产前诊断

目的探讨微柱凝集技术在诊断母子ABO血型不合的产前应用。方法采用BioVue系统微柱凝集技术,对200例丈夫血型为非O型的O型血孕妇检测血清IgG抗体效价。结果产后婴儿母子ABO血型不合新生儿溶血性疾病(HDN)发病率随母亲血清IgG抗体效价增高而上升。结论BioVue系统微柱凝集技术能准确检测孕妇血清IgG抗体效价,是产前预报因母子ABO血型不合而引起HDN的可靠方法。     

1例罕见AB3亚型的分子机制

目的探讨1例罕见AB3亚型的分子机制。方法对1例ABO血型正反定型不符患者的血型通过血清学鉴定、PCR-SSP、ABO基因直接测序、TA克隆单体型分析等方法分析其分子机制。结果该患者血型鉴定为比较少见的A102/B301亚型,该亚型是由于ABO基因第七外显子存在1054C/T杂合导致R352W氨基酸改变所致。结论 ABO基因存在1054CT,该突变可能是导致B301亚型的分子遗传基础之一。关健词:     

尿道下裂与ABO血型的相关性分析

目的探讨尿道下裂与ABO血型之间的相关性。方法对1997年1月1日至2008年9月1日中山大学第二附属医院收治的633例尿道下裂患者血型进行回顾性分析,并与健康男性进行对照,两组均采用试管凝集法检测其ABO血型,统计出两组的ABO血型分布、频率及相对危险率,用卡方(χ2)检验进行统计学处理。结果尿道下裂患者血型比例分别为A型27.33%、B型28.44%、AB型6.63%、O型37.60%,ABO血型分布为OBAAB,与对照组ABO血型分布特征差异有统计学意义(P=0.0020.05),经分割χ2检验发现患者组和对照组两组人群的O型血的频率分布差异有统计学意义(P=0.0040.0167);相对危险率统计分析结果显示,O型血是尿道下裂的易感因素,与其他几种血型比较差异有统计学意义(P0.001)。结论尿道下裂的发生与ABO血型存在一定的相关性,O型者可能易患尿道下裂。     

天然ABO抗体缺失的分子机制研究

目的 从ABO基因水平探讨正常人群血型鉴定中出现天然抗体缺失的机制.方法 部分或全部缺失抗体导致ABO血型正反定型不一致的15个正常健康个体运用检验红细胞表面血型抗原最为敏感的方法-吸收放散试验未能检测出其抗原性.PCR扩增后直接序列分析ABO基因全长编码区及5'端调控(CBF/NF-Y)增强子区域多态性.结果 在常规的血清学反向定型实验中,这15个标本的血清中部分或全部缺失抗体,吸收放散实验与A或B型红细胞反应均为阴性.12个标本的ABO基因分析定型、增强子区域多态性与血清学结果相符合,1个O表型的标本中存在B101等位基因,2个B表型标本中检测出A102等位基因.结论 天然ABO抗体缺失的分子机制可能部分被揭示:ABO基因编码少量表达抗原,按照天然抗体产生规律,导致不能产生相应的ABO抗体.ABO基因分型在正反定型不符的疑难血液标本鉴定中,是血清学方法 非常有益的补充手段,是正确鉴定血型的有效方法.     

基因扫描技术在ABO血型基因型鉴定中的应用

目的 建立基因扫描（GeneScan）技术鉴定ABO血型基因型的方法。方法根据O1基因在261位G缺失而非O1基因无缺失，B基因第803位核苷酸与A基因不同的特点，设计4对引物，分别在上游引物5’端标记荧光基团，PCR-SSP扩增后，用基因扫描技术鉴定ABO血型基因型。结果129例样本用Genescan技术鉴定的ABO血型结果与血清学结果完全符合，A、B、O基因频率分别为0．198、0．159、0．643。其中AA基因型8例（6．2％），AO基因型29例（22．5％），AB基因型6例（4．7％），BB基因型6例（4．7％），BO基因型23例（17．8％），OO基因型57例（44．2％）。结论Genescan技术鉴定ABO血型基因型具有可行性，提供了一种可选择的方法。     

ABO新生儿溶血病与O型孕妇血清中IgG及其亚类含量的相关分析

目的探讨O型孕妇血清IgG抗体及其亚型含量与新生儿溶血病(HDN)的关系。方法采用血型血清学方法,对317名夫妇血型不合的O型孕妇作IgG抗体效价检测,并对其中有妊娠史的287名孕妇作IgG抗体水平比较;采用ELISA法对71名HDN患儿及其母亲、65名健康O型孕妇和51名健康新生儿的IgG亚类作定量分析。结果1)317名新生儿中发生ABOHDN71例(22.4%),其中抗-A46例、抗-B25例;2)随着妊娠次数的增加,IgG抗体效价≥64者的比例和ABO-HDN发病率升高,>2次妊娠与第2次妊娠间有统计学差异;3)患儿及其母亲体内IgG抗体的含量显著高于正常对照组,且以IgG1抗体为主,患儿体内的IgG1比例(61.9%)高于母体(52.8%)。结论新生儿ABOHDN的发病率随其母亲体内IgG抗体效价的升高而升高,且与母亲体内IgG1呈正相关。夫妇血型不合的O型孕妇应定期检测IgG抗体及其亚类含量。     

Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran

ObjectiveTo investigate correlations between ABO/rhesus (Rh) blood group antigens and anti-Helicobacter pylori and anti-cytotoxin-associated gene A (CagA) seropositivity in blood donors.MethodsA total of 311 blood donors were enrolled. ABO and Rh blood groups were determined using hemagglutination tests. Specific anti-H. pylori IgG and anti-CagA IgG antibodies in sera were quantitated by enzyme-linked immunosorbent assay. Correlations between blood groups and anti-H. pylori and anti-CagA seropositivity were evaluated using the Chi-square test.ResultsO+ was the most frequent blood type (38%, n = 118). Anti-H. pylori IgG seropositivity was observed in 240 (77.2%) blood donors, while anti-CagA IgG seropositivity was observed in 132 (42.5%) blood donors. Although seropositivity rates for both anti-H. pylori and anti-CagA IgG were higher in individuals with blood type O, no statistically significant associations were observed between seropositivity and any ABO/Rh blood groups.ConclusionIndividuals with blood type O may have higher rates of H. pylori seropositivity.