离子对提取分光光度法测定盐酸乙胺丁醇片含量

盐酸乙胺丁醇采用非水滴定法测定其含量。但其制剂盐酸乙胺丁醇片由于辅料的影响，用非水滴定法测定颇为繁杂，处理费时，试剂费用高，也不适于药厂快速分析。有机碱阳离子在水溶液中可与加入的当量阴离子形成离子对(络合物)。据此，本文以溴甲酚绿为反离子，在pH43的介质中与乙胺丁醇形成稳定的有色离子对，用氯仿提取，在420nm处测定吸收度。此法操作简便，分析快速，灵敏度高。     

紫外分光光度法测定枸橼酸西地那非片含量

枸橼酸西地那非片是治疗勃起功能障碍药 ,其原料的含量测定为非水滴定法 ,片剂的含量测定有高效液相色谱法。作者利用西地那非在 0 .1ｍｏｌ/Ｌ盐酸溶液中 ,在紫外光区 2 92ｎｍ波长处有最大吸收峰的特性 ,采用紫外分光光度法对自制片剂进行含量测定 ,方法准确可靠 ,操作简便。1　仪器与试药　　ＵＶ 16 0 1紫外分光光度计 (日本岛津公司 ) ,ＢＳ110Ｓ电子天平 (北京赛多利斯天平有限公司 ) ,枸橼酸西地那非原料 (上海华拓医药有限公司 ) ;枸橼酸西地那非片 (本院自制 ,批号 :0 2 0 2 0 8、0 2 0 4 16、0 2 0 80 6 )。盐酸为分析纯。2　方法…     

盐酸苯丙哌酮片含量的酸性染料比色测定法

盐酸苯丙哌酮片含量的酸性染料比色测定法附属医院药剂科谭才宏，马乔春药剂教研室辛淮生，柳春华镇江焦化厂卫生所刘玉芬盐酸苯丙哌酮片（商品名为易咳嗪Ⅰ）是祛痰镇咳药物。其原料和片剂的含量测定有非水滴定法、紫外分光光度法。因受操作繁琐和辅料的影响，方法不尽理...     

酸性染料比色法测定维生素B1片剂的含量

维生素B_1的含量测定方法应用较为普遍的有硅钨酸重量法、荧光法、非水滴定法及比色法。中国药典1995年版采用紫外分光光度法对其片剂中的维生素B_1的含量进行了测定。本文利用维生素B_1在pH3.80条件下与溴甲酚绿形成易被氯仿提取的离子对,在波长416nm处测定吸收值进行定量,具有取样量少,操作简单的特点,测定结果与中国药典法(1995年版)一致。     

替莫唑胺片的制备及含量测定

目的：制备替莫唑胺片剂。方法：用乳糖等辅料制备了替莫唑胺片，采用紫外分光光度法进行含量测定。检测波长329mm,线性范围2-18μg/mL，r=，建立了含量测定方法。结果：含量测定平均回收率为99.89%,RSD=0.82%(n=9)。结论：制备该片剂工艺简单，含量稳定，质量可控。     

复方吗啉胍片的非水滴定法

吗啉胍为一抗病毒药物。一般都利用其分子中两个胍基的碱性,采用非水滴定法进行原料或片剂的含量测定。由于片中存在的少量山莨菪碱消耗滴定液,以致干扰测定。但目前往往忽略这项干扰。吗啉胍两个胍基的碱性不同,在水溶液中一分子吗啉胍只与一分子盐酸     

紫外分光光度法控制盐酸吗啉脒胍半成品的含量

盐酸吗啉胍片为抗病毒药,其原料药和片剂的含量测定方法均为非水滴定法,操作繁琐费时,根据盐酸吗啉脒胍水溶液在237nm波长处有最大吸收,采用紫外分光光度法测定其片剂的含量,获得满意的结果。     

分光光度法测定氟康唑片的含量

氟康唑(Ⅰ)为新型三唑类抗真菌药，能特异有效抑制真菌的甾醇合成，口服本品对动物真菌感染：如念珠菌感染、新型隐球菌感染、小孢子菌属感染及毛癣菌属、表皮癣菌、糖秕孢子菌等感染均有效。(Ⅰ)的含量测定采用非水滴定法，本文根据(Ⅰ)紫外吸收光谱特性，采用分光光度法测定氟康唑片的含量。本法简便，快速，结果满意。     

紫外分光光度法测定吡哌酸及其片剂的含量

吡哌酸(Pipemdic acid)是广泛应用于临床的喹诺酮类抗菌药。本品原料药和片剂的含量测定,中国药典90年版分别采用非水滴定法和对照品比较法,本文根据吡哌酸在275±1nm波长处有最大吸收,测定了吡哌酸吸收系数值,并拟定了紫外分光光度法测定吡哌酸含量,经回收率试验,结果满意,方法简便、准确灵敏。     

交流示波极谱滴定法测定枸橼酸维静宁及其片剂的含量

枸橼酸维静宁(Pentoxyverine Citrate)又名咳必清,是我国药典收载的一种镇咳药。其原料药含量测定方法为非水滴定法,片剂需用剥去糖衣的片粉碱化后经氯仿提取,再用非     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

股骨远端粉碎性骨折双板固定的生物力学研究

目的探讨治疗股骨远端粉碎性骨折的新方法。方法应用10件成人鲜尸股骨,造成股骨远端髁间、髁上粉碎性骨折,内侧采用聚乙烯板、外侧采用钢板双向固定,分别作压缩、弯曲、扭转等生物力学实验研究。另取10件标本进行单向外侧钢板固定作为对照。结果双向固定组抗弯曲、扭转能力显著增强(P<0.01),抗压缩能力也较单侧固定组强(P<0.05)。结论双向固定治疗股骨远端粉碎性骨折有明显的应用价值。     

Cloning and sequence analysis of β-actin gene from Aedes albopictus （Diptera： Culicidae）

Objective: To obtain the complete β-actin gene from Aedes albopictus. Methods: Total RNA was extracted from C6/36 cells. Degenerate primers were designed based on the β-actin sequences of An. gambiae, Ae. aegypti, Cx. pipiens pallens and D.melanogaster. By RT-PCR, the product was amplified, purified, cloned into the pGT vector and sequenced. The β-actin sequence was aligned and phylogenetically analyzed by the BLAST program and the CLUSTAL W program. Results: A sequence of 1132 bp including an open reading frame of 1131 bp was obtained (GenBank DQ657949). The deduced protein had 376 amino acids.Aligned to SWISS-PROT, it exhibited a high level of identity with β-actins from Anopheles, Drosophila and Culex at the amino acid sequence level. Phylogenetic analysis indicated that Ae. albopictus β-actin was much more homologous with invertebrate β-actin than with vertebrate β-actin. Conclusion: The gene may be used as the internal control in the experiments of Ae. albopictus.     

Subjective sleep quality in perimenopausal women and its related factors

Objective To evaluate the sleep quality and its related factors among perimenopausal women. Methods A self-designed questionnaire was administered to 506 perimenopausal women. The questionnaire included the influencing factors on the sleep quality, the Pittsburgh Sleep Quality Index (PSQI), the Zung Self-Rating Depression Scales (SDS), the Zung Self-Rating Anxiety (SAS) and the Modified Kupperman Index (KI). Data were analyzed by SPSS11.5. Results The mean PSQI was 5.97±4.30.Twenty-four percent of perimenopausal women reported poor sleep. Age and perimenopausal symptoms were significantly correlated with sleep quality. The sleep quality of the 45～49 age group was the poorest and the 40～44 age group was the best. The women who had higher Kupperman index were more likely to be poor sleepers. There was no significant correlation between occupation and sleep quality. Night sweat, depression, anxiety, hot flash, stressful life event, and regular exercise were significantly and independently related with sleep quality. Among them, regular exercise was a protective factor of sleep quality. ConclusionHigh incidence of poor sleep quality exists among perimenopausal women. Some effective interventions should be taken to improve the sleep quality of perimenopausal women.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

基层医院急性心肌梗死249例临床用药分析

近年来,随着我国经济社会的发展,冠状动脉粥样硬化性心脏病（冠心病）发病率明显升高,已成为威胁我国人民健康的重大疾病。急性心肌梗死是冠心病的重要类型,具有病死率高。致残率高的特点。其治疗的关键在于尽早。充分及持续开通梗死相关血管。大量临床病例已经证实早期再灌注治疗和早期使用受体阻滞剂（BB）。血管紧张素转换酶抑制剂（ACEI）。     

Treatment of 256 cases of Osteoarthritis of Knee Joint with Guo Jianhua''''s Four-step Therapy

256 cases of osteoarthritis of knee joint were treated with Professor Guo Jianhua's four-step therapy,i.e.acupuncture at five points in the knee to remove obstruction of collaterals and stop pain,massotherapy to separate adhesion and relieve spasm,fumigation and steaming with hot decoction to promote blood circulation and expel swelling,and functional exercises to consolidate the therapeutic effects.121 cases were cured,117 cases markedly effective,and 18 cases ineffective,with a total effective rate of 92.97%.