慢性特发性荨麻疹患者外周血单一核细胞干扰素-γ受体mRNA表达

目的探讨慢性特发性荨麻疹(CIU)患者外周血单一核细胞(PBMC)干扰素-γ受体(IFN-γR)mRNA表达情况。方法收集45例CIU患者,38例正常人作为对照,采集PBMC提取RNA,应用逆转录聚合酶链扩增(RT-PCR)法检测IFNR mRNA表达水平。结果CIU患者PBMC中Th1细胞因子受体IFN-γR mRNA表达水平(0.636±0.194),高于健康对照组(0.540±0.184)(t=2.315,P(0.05)。结论CIU患者PBMC中IFN-γR的mRNA表达增高,可能与该病发病机制有关。     

Toll样受体2 mRNA在梅毒患者外周血单一核细胞的表达

目的研究一期梅毒、二期梅毒、早期潜伏梅毒患者驱梅治疗前、后外周血单一核细胞(PBMCs)Toll样受体2(TLR2)mRNA的表达情况。方法采用实时定量PCR方法检测一期梅毒、二期梅毒、早期潜伏梅毒患者各15例驱梅治疗前和驱梅治疗结束后3个月PBMCs中TLR2mRNA的表达水平,并与20例正常人作对照。结果驱梅治疗前一期梅毒、二期梅毒及早期潜伏梅毒患者与正常对照组TLR2mRNA表达水平比较差异有统计学意义(P<0.001);一期梅毒、二期梅毒及早期潜伏梅毒组之间比较,差异无统计学意义(P>0.05);经驱梅治疗后,一期梅毒、二期梅毒及早期潜伏梅毒患者PBMCs中TLR2mRNA的表达水平显著下降,与驱梅治疗前比较,差异有统计学意义(P<0.001);与正常对照组比较,差异无统计学意义(P>0.05);TLR2mRNA的表达水平与血清RPR滴度不存在相关关系(P>0.05)。结论TLR2可能参与了免疫细胞对梅毒螺旋体的识别和信号转导,并在梅毒的发生、发展过程中可能起重要作用。     

天疱疮患者外周血单个核细胞瘦素受体mRNA的表达

目的:探讨瘦素受体mRNA水平的表达在天疱疮发病中的作用.方法:采用逆转录-聚合酶链反应(RT-PCR)技术对天疱疮患者和正常对照组外周血单个核细胞(PBMC)内短型瘦素受体(OB-Ra)和长型瘦素受体(OB-Rb)mRNA表达进行了检测.结果:天疱疮患者和正常对照组PBMC内均存在OB-Ra和OB-Rb mRNA的表达;天疱疮患者OB-Ra mRNA和OB-Rb mRNA的表达均较正常对照组减弱(P<0.05);与中、轻症相比,重症组OB-Ra mRNA和OB-Rb mRNA的表达均减弱,但无统计学意义(P>0.05).结论:天疱疮患者存在瘦素受体mRNA水平表达减弱,这种异常可能参与了天疱疮的发病.     

白癜风患者外周血单一核细胞Toll样受体7、9 mRNA表达的初步研究

目的 探讨Toll样受体7、9 （TLR7、TLR9）mRNA在白癜风患者外周血单一核细胞（PBMC）中的表达及其意义。方法 应用实时荧光定量逆转录聚合酶链反应（RT-PCR）技术检测50例白癜风患者及25例正常人对照PBMC中TLR7、TLR9 mRNA的表达。采用SPSS11.5软件进行统计学分析,两样本均数比较采用t检验。结果 白癜风患者PBMC中TLR7 mRNA（目的基因绝对定量校正拷贝数为0.85 ± 1.90）、TLR9 mRNA（0.94 ± 2.25）表达均明显高于对照组（分别为0.44 ± 1.18和0.11 ± 0.31,P ＜ 0.05和 ＜ 0.01）;稳定期与进展期、局限型与泛发型白癜风患者TLR7、TLR9 mRNA表达差异均无统计学意义（P值均 ＞ 0.05）。结论 白癜风患者PBMC中TLR7、TLR9 mRNA的表达均上调,TLR7、TLR9的异常表达可能参与白癜风的发病机制。     

早期梅毒患者外周血单一核细胞Toll样受体2的表达

目的：观察早期梅毒患者外周血中TOLL样受体2表达水平的变化，探讨TLR2在早期梅毒免疫学发病机制中的作用。方法：采用双色免疫荧光抗体染色流式细胞仪检测59例梅毒患者外周血TLR2的表达水平，以20例正常人作为对照组。结果：早期梅毒患者TLR2表达的阳性率显著低于正常对照组，二者差异有统计学意义(P﹤0.001)；显性梅毒患者外周血TLR2表达的阳性率显著低于隐性梅毒组，差异有统计学意义(P﹤0.001)。结论：在显性梅毒患者外周血中TLR2的表达水平低下，而在隐性梅毒患者组中TLR2的表达水平与正常对照组相似，提示显性梅毒患者体内可能缺乏非特异性免疫反应，TLR2可能反映了机体的免疫功能状态。     

Toll样受体2、4在麻风患者外周血单一核细胞中的表达

目的 探讨Toll样受体2、4 mRNA在麻风患者外周血单一核细胞中的表达.方法 运用SYBR Green荧光定量RT-PCR技术检测麻风现症患者与已痊愈患者各30例外周血单一核细胞中Toll样受体2、4 mRNA的表达水平,并用正常人作对照.结果 Toll样受体2 mRNA在麻风患者外周血单一核细胞中表达明显高于痊愈组和正常人对照组(P＜0.01).Toll样受体4 mRNA在麻风现症患者外周血单一核细胞中的表达与痊愈组和正常人对照组差异无统计学意义(P＞0.05).结论 Toll样受体2 mRNA在麻风患者外周血单一核细胞的表达明显升高,提示Toll样受体2 mRNA可能是麻风杆菌的特异识别受体.     

天疱疮患者外周血单一核细胞IL-6受体TNFα受体mRNA的表达

目的:研究天疱疮患者外周血单一核细胞(PBMCs)促炎性细胞因子受体mRNA的表达及其意义。方法:用逆转录-聚合酶链反应(RT-PCR)方法,对37例寻常型天疱疮患者及30例健康对照者PBMCs上IL-6受体、TNFα受体mRNA进行检测。结果:所有天疱疮患者及健康对照者均可检测到IL-6受体及TNFα受体mRNA,IL-6受体mRNA表达量在患者组与对照组之间无显著性差异,TNFα受体mRNA的表达量在患者组为0.50±0.19,对照组为0.22±0.10,患者组的表达量显著高于对照组(P<0.001)。结论:天疱疮患者PBMCs的TNFα受体mRNA表达升高,可能在天疱疮的发病机制中起一定的作用。     

系统性红斑狼疮患者外周血单一核细胞Toll样受体4及2mRNA表达的研究

目的 研究系统性红斑狼疮(SLE)患者与正常人外周血单一核细胞Toll样受体4(TLR4)mRNA、TLR2mRNA的表达情况。方法 运用Taqman实时定量PCR方法检测活动期SLE患者28例、稳定期SLE患者13例外周血单一核细胞TLR4、TLR2的mRNA表达水平,并与正常人作对照。结果 活动期SLE患者外周血单一核细胞TLR4mRNA较正常人和稳定期SLE患者表达水平显著升高(P<0.01);其中复发的、经糖皮质激素治疗的活动期SLE患者较未经治疗的初发患者TLR4mRNA升高更明显(P<0.05)。SLE活动期和稳定期患者TLR2mRNA较正常人对照显著降低(P<0.01)。结论 活动期SLE患者TLR4mRNA表达水平升高,尤其是使用过糖皮质激素的患者TLR4升高更为显著,其原因可能与感染作为诱发SLE活动因素之一有关。     

银屑病患者外周血单一核细胞中趋化因子受体CXCR1及CXCR2 mRNA的表达

目的:探讨银屑病患者外周血单一核细胞(PBMC)中CXC型趋化因子受体CXCR1及CXCR2的表达水平及其临床意义。方法:应用逆转录-聚合酶链反应(RT-PCR)法,检测了进行期斑块状银屑病患者33例及其中16例患者治疗后PBMC中CXCR1及CXCR2 mRNA的表达,设30名健康人作为正常对照,并将检测结果与患者皮损面积及严重程度指数(PASI)进行了相关性分析。结果:斑块状银屑病患者PBMC中CXCR2 mRNA表达水平为1.38±0.87,明显高于健康人对照组0.73±0.58,治疗后患者1.21±0.62仍高于健康对照组(P<0.05);CXCR1 mRNA表达水平为0.26±0.28,与健康对照组及同一组患者治疗后相比均无明显差异,分别为:0.17±0.26,0.27±0.31(均P>0.05);银屑病患者PBMC中CXCR2 mRNA水平高于CXCR1 mRNA水平,二者均与PASI之间无明显相关性。结论:CX-CR2可能参与了银屑病的发病机制。     

系统性红斑狼疮患者外周血单一核细胞趋化因子受体2和3 mRNA的表达

目的探讨趋化因子受体(CCR)2及CCR3在系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中的表达及与疾病的相关性.方法对93例SLE患者和30名健康对照者,应用半定量逆转录(RT)-PCR方法检测其CCR2、CCR3 mRNA表达水平,并应用SLE疾病活动性指数评分标准(SLEDAI)对SLE患者进行评分.结果①9例SLE患者活动期、非活动期及对照组PBMC CCR2与CCR3 mRNA表达水平分别为2.989±1.595与2.847±2.400、0.388±0.213与0.585±0.219、0.263±0.157与0.602±0.124,活动期与非活动期、对照组相比差异均有显著性(P值分别为0.013,0.004;0.000,0.007);非活动期与对照组相比差异无显著性(P＞0.01).②SLE患者PBMC中CCR2、CCR3 mRNA表达水平与SLEDAI分别作直线相关性分析,CCR2 mRNA、CCR3mRNA水平与SLEDAI均呈正相关.③具有肌炎临床表现的SLE患者其PBMC CCR2、CCR3表达水平较无肌炎表现时降低.血小板减少的SLE患者比不具有相应症状者CCR2 mRNA表达降低.结论SLE患者PBMC CCR2、CCR3表达均增加,且与疾病活动性呈正相关,而CCR2、CCR3为表达于Th2细胞的趋化因子受体,提示SLE患者Th2细胞占优势.CCR2可能参与SLE患者肌炎及血小板减少的发生,CCR3参与肌炎发生.     

Evaluation of H2 receptor antagonists in chronic idiopathic urticaria

H1-antagonist (hydroxyzine hydrochloride) in dosage of 10 mg-25 mg thrice a day failed to elicit satisfactory response in 60 out of 170 patients of chronic idiopathic urticaria. Additional administration of H2-antagonist (cimetidine) in dosage of 200 mg four times a day, in patients not responding earlier to H1-antagonist alones exhibited moderate to good improvement of various parameters of urticaria in approximately 85% patients.     

慢性特发性荨麻疹患者单一核细胞IL-2、4、10受体mRNA的表达

目的 探讨白介素2受体(IL-2R)、白介素4受体(IL-4R)、白介素10受体(IL-10R)在慢性特发性荨麻疹(CIU)患者外周血单一核细胞(PBMC)中的表达及与疾病的相关性.方法 收集30例确诊的CIU患者和30例正常人对照,收集PBMC,提取RNA.应用逆转录-聚合酶链反应(RT-PCR)方法检测研究对象IL-2R、IL-4R、IL-10RmRNA表达水平.结果 CIU患者IL-10RmRNA表达水平比健康人对照组增高,两组分别为0.806±0.072、0.373±0.027,差异有统计学意义(t=5.62,P<0.01).而IL-2R、IL-4RmRNA表达水平患者组与健康人对照组无统计学意义,分别为,IL-2R:0.688±0.357、0.670±0.527,t=1.16,P>0.05;IL-4R:0.679±0.055和0.549±0.049,t=1.77,P>0.05.结论 CIU患者IL-10RmRNA表达水平比健康人对照组增高,而IL-2R、IL-4RmRNA表达水平与健康人对照组无统计学意义.CIU患者存在T淋巴细胞细胞因子受体表达的异常.     

SLE患者PBMC中IL-6mRNA和IL-10mRNA的表达

为了进一步了解TH2型细胞因子IL-6和IL-10在系统性红斑狼疮（SLE）患者外周血单核细胞（PBMC）中的基因表达情况，对7例SLE患者和4例年龄相匹配对照PBMC中IL-6mRNA和IL-10MRNA表达进行了原位杂交检测，并对检测结果进行计算机图象灰度扫描分析、结果SLE患者PBMC中IL-6mRNA和IL-10mRNA均明显高表达，并与患者ANA滴度及抗（ISDNA抗体有一定的相关性尤以IL-6为著。提示IL-6和IL-10作为B细胞刺激因子在SLE发病中具有重要病理性作用，阻止或减少其分泌可能有助于SIE治疗。     

Comparison of chronic autoimmune urticaria with chronic idiopathic urticaria

BACKGROUND: Chronic urticaria has been described in patients with Helicobacter pylori infection. Despite numerous studies, the correlation between H. pylori infection and chronic urticaria is doubtful. Our study was performed to determine the prevalence of H. pylori infection in autoimmune urticaria and in patients suffering from autoimmune urticaria and autoimmune thyroiditis. METHODS: The authors widely investigated 48 patients. The examinations were extended principally to autologous serum skin test, antithyroid antibodies, and the presence of H. pylori infection as well as detection of antibodies against H. pylori. RESULTS: Out of the 48 patients, 26 were regarded as having autoimmune origin. The prevalence of antithyroid antibodies was different in the two groups of patients with urticaria. There were 11 patients (42.3%) in the autoimmune group compared with three patients (13.6%) in the nonautoimmune group with antithyroid peroxidase antibody (P = 0.03). The difference in the prevalence of H. pylori infection was significant between autoimmune urticaria with and without thyroid autoimmunity (90.9% vs. 46.7%; P = 0.02). Autoimmune thyroiditis was connected with CagA +H. pylori strains, as the H. pylori- specific IgG antibodies revealed significant differences in a prevalence of 120 kDa (P < 0.05). CONCLUSIONS: The authors observed a relationship between autoimmune urticaria and autoimmune thyroiditis. The results strengthen the possibility of cross-reactivity being triggered between CagA plus H. pylori strains and some other organ-specific autoimmune diseases such as autoimmune urticaria and autoimmune thyroiditis. This indicates a possible role of H. pylori in triggering autoimmune urticaria in at least a select group of patients.     

Cathepsin G在慢性自发性荨麻疹患者血清中的表达

目的:研究组织蛋白酶G(cathepsin G)在慢性自发性荨麻疹患者血清中的表达变化,并初步探讨其作用。方法:纳入30例就诊前未经治疗的慢性自发性荨麻疹患者,设为观察组;另纳入30例健康体检者作为对照组。ELISA法检测两组血清中cathepsin G表达水平并进行比较。结果:对照组血清中cathepsin G浓度为(32.2±12.3)ng/m L,慢性自发性荨麻疹患者血清中cathepsin G浓度上调为(260.4±26.9)ng/m L,差异有统计学意义(t=42.26,P0.01)。结论:慢性自发性荨麻疹患者血清cathepsin G表达明显上调,其可能通过与多种细胞因子及炎症介质相互作用,参与慢性自发性荨麻疹的发病机制。     

Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria

Background The pathophysiological mechanism of chronic urticaria is still poorly understood and its aetiology is considered to have an autoreactive basis. Autologous serum skin tests (ASSTs) and autologous plasma skin tests (APSTs) comprise the simplest ways for diagnosing autoreactive urticaria (with autoantibodies, histamine‐releasing factor and coagulation factors, especially thrombin) in a clinical setting. However, there are still some questions about the specificity of these tests. Objectives To evaluate the role of autoreactivity in the pathogenesis of chronic urticaria by means of measuring plasma prothrombin fragments 1 + 2, which are used as markers of thrombin, and to compare the APST with the ASST. Methods Forty‐two patients (19 men and 23 women; mean age 35·7 years, range 28–76) and 35 healthy volunteers (19 men and 16 women; mean age 30·3 years, range 20–80) were included in the study. APST, negative (ASST, sodium citrate, saline) and positive (histamine) control tests were performed in the patient and control groups. The levels of plasma prothrombin fragments 1 + 2 were also assessed. Results When the APST was evaluated without negative controls, it was positive in 67% of patients. However, the APST was positive in 0% when it was evaluated with negative controls. Levels of prothrombin fragments 1 + 2 were found to be elevated in patients with chronic idiopathic urticaria. Conclusions We suggest that as negative control tests were not performed along with the APST in previous studies, the APST showed a high rate of positivity. Thus, the use of APST for evaluating autoreactivity in clinical practice is not superior to the use of ASST and further studies should be conducted.     

慢性荨麻疹患者皮肤组织IL-4和eotaxin的表达

目的:检测细胞因子IL-4与约塔辛(eotaxin)在慢性荨麻疹(CU)患者皮肤组织中的表达.方法:采用免疫组织化学染色方法检测24例CU患者和12例健康对照皮肤组织中IL-4与eotaxin的表达.结果:CU患者皮损组织IL-4和eotaxin的表达均高于健康对照(P<0.001);患者正常皮肤组织eotaxin的表达显著高于健康对照(P<0.01).结论:迟发相变态反应可能参与慢性荨麻疹的发病环节.     

Desloratadine 5 mg once daily improves the quality of life of patients with chronic idiopathic urticaria

BACKGROUND: Chronic urticaria is known to debilitate a person's quality of life via sleep disruption, itching lesions, fatigue, social isolation, energy loss and emotional/sexual difficulties. Once-daily desloratadine significantly improved the signs and symptoms of CIU. OBJECTIVE: Assess the effect of desloratadine 5 mg once daily on the quality of life of patients suffering of chronic idiopathic urticaria (CIU). Study population One-hundred twenty-one consecutive patients with CIU present for at least 6 weeks prior to inclusion and with a current flare of at least 3 weeks, were included in the study in 24 Belgian centres. RESULTS: The mean dermatology life quality index (DLQI) significantly decreased from baseline to day 7 and further to day 42. Sixty per cent and 77% of patients had a clinically significant change (i.e. a decrease of at least 2 points) at day 7 or day 42, respectively, as compared with that of day 0. Change in pruritus and size of the hives significantly correlated with the change in the score of the quality of life. One-third of patients experienced complete relief whereas in 1 of 10 patients no effect was experienced. CONCLUSIONS: Desloratadine significantly improves the quality of life of patients with chronic idiopathic urticaria as reflected by the dermatology life quality index (DLQI).