短节段腰椎滑脱复位固定器的生物力学评价

目的评价腰椎滑脱复位固定器(HOIST)的生物力学性能。方法采用7具新鲜成人尸体脊柱标本(L2～S2),Panjabi法制作腰椎滑脱模型,固定器械分别为HOIST器械和DICK器械。应用脊柱三维运动稳定测试仪对标本进行前屈,后伸,右、左侧弯及左、右轴向旋转6个方位的运动范围(ROM)测试;在858MTS实验机上进行疲劳实验。实验分为完整状态组、L4滑脱组、HOIST器械固定组、HOIST器械疲劳组和DICK器械组。结果滑脱状态的ROM明显大于其他4个状态(P<0.05);HOIST器械固定疲劳前后的标本(L4,5)在6个方位的ROM明显较完整状态小(P<0.05);HOIST器械固定疲劳前后以及DICK器械固定三个状态间的ROM(除左轴向旋转外)差异无显著性意义(P>0.05)。结论HOIST器械在力学性能上能满足生理需要,且具有良好的疲劳后稳定性。     

椎体成形在胸腰椎压缩性骨折后的三维稳定性测试

目的评估在胸腰椎骨折后椎体成形术对恢复脊柱单元即刻三维稳定性的作用。方法7具新鲜胸腰段脊柱标本。测试前屈、后伸、左侧弯、右侧弯、左旋转、右旋转的中性区（neutral zone,NZ）和运动范围（range of motion,ROM）。程序：①完整状态;②骨折后状态;③椎体成形后;④3000次循环疲劳后。结果骨折后中性区和运动范围均明显增大。椎体成形后屈伸、侧弯、旋转在NZ及ROM均明显减少。疲劳后虽然有增加,但较骨折后明显减少。运动范围在椎体成形后和损伤前完整时比较无差别。结论骨水泥椎体成形在离体常规负荷下可恢复脊柱运动单元的三维稳定性。     

胸腰椎爆裂性骨折伤椎固定的生物力学研究

目的 比较跨节段椎弓根钉固定与三椎体六枚椎弓根钉固定术固定胸腰椎骨折的生物力学效果。方法 采用8具新鲜小牛椎体标本（T11-L3）,MTS机压缩制作L1椎体爆裂性骨折模型。实验分完整状态组、骨折组、跨节段椎弓根钉固定组、三椎体六枚椎弓根钉固定组。应用脊柱三维运动试验机对标本进行前屈,后伸,左、右侧弯及左、右旋转6个方位的运动范围（ROM）测试并计算刚度值,所得数据进行统计学处理,比较各组间差异。结果 脊柱骨折状态6个方向ROM均显著增加（P〈0．05）,椎间刚度值明显降低（P〈0．05）;固定状态的ROM均较完整状态、骨折状态显著减小（P〈0．01）,而刚度值均较完整状态、骨折状态增大,差异有显著性意义（P〈0．05）;跨节段椎弓根钉固定组与三椎体六枚椎弓根钉固定组之间的ROM、刚度值差异均无显著性意义（P〉0．05）。结论 跨节段椎弓根固定与三椎体六枚椎弓根钉固定术在重建脊柱骨折稳定性方面,效果无明显差异。     

低温等离子体消融术对山羊胸椎前路松解的生物力学观察

目的:比较低温等离子体消融术去除胸椎间盘和传统胸椎间盘切除术对山羊胸椎生物力学的影响,评估低温等离子体消融术前路松解的可行性和有效性.方法:将16只2～2.5月龄的健康雌性山羊随机分为低温等离子体消融组(6只)、传统胸椎间盘切除组(6只)、空白对照组(4只,其椎间盘未做任何处理),手术组动物均经前路处理中段6个胸椎间盘(T5～T11),术后处死动物,所有动物均取下完整的T4～T12胸段脊柱标本,应用TMT 858型生物力学试验机测试标本柔韧度和运动范围(ROM),包括中性区(NZ)和弹性区(EZ).结果:松解后脊柱标本的柔韧度和ROM与对照组相比均有显著增加(P<0.05);消融组左、右侧弯和左、右旋转的柔韧度与传统组比较无显著性差异(P>0.05),但前屈、后伸时两组之间有显著性差异(P<0.05);消融组后伸、左侧弯和左、右旋转的ROM与传统组比较无显著性差异(P>0.05).前屈、右侧弯时两组之间有显著性差异(P<0.05);松解组后伸,左、右侧弯和左、右旋转的NZ与对照组比较有显著性差异(P<0.05),而消融组前屈、后伸、左侧弯和左、右旋转的NZ与传统组比较无显著性差异(P>0.05),消融组右侧弯的NZ与传统组比较有显著性差异(P<0.05);消融组后伸、传统组右侧旋转的EZ与对照组比较均有显著性差异(P<0.05),而消融组的EZ与传统组比较无显著性差异(P>0.05).结论:采用低温等离子体消融术去除胸椎间盘可以获得和传统胸椎间盘切除术对胸椎前路松解相似的效果.     

两种植骨法对胸腰椎爆裂骨折复位后骨缺损空隙残存率及压缩刚度的影响北大核心CSCD

目的研究胸腰椎爆裂骨折两种方法植骨后伤椎椎体的植骨量、骨缺损空隙残存率及生物力学稳定性。方法取18个4～6月龄新鲜小牛脊柱腰段(L1～5)离体标本,制备L3椎体爆裂骨折模型,模拟胸腰椎爆裂骨折行伤椎撑开复位、椎弓根螺钉内固定。将18个标本随机分为3组,每组6个,A组伤椎椎体内不植骨,B组行经双侧椎弓根伤椎椎体内植骨,C组行经单侧椎管伤椎椎体内植骨。记录B、C组植骨量;将3组标本行DR片及CT观察伤椎椎体骨缺损空隙大体情况;经CT扫描后采用数格子法计算伤椎椎体骨缺损空隙残存率;应用ElectreForce-3510高精度生物材料试验机测试标本压缩刚度。结果 B、C组植骨量分别为(4.58±0.66)g和(5.72±0.78)g,比较差异有统计学意义(t=2.707,P=0.022)。DR片及CT观察示:A组标本伤椎椎体内见较大骨缺损空隙;B组伤椎椎体的"蛋壳样"空隙内可见骨粒填充,多集中于伤椎椎体后半部,椎体前部填充不足;C组伤椎椎体内较多骨粒填充,分布均匀。A、B、C组标本骨缺损空隙残存率分别为52.0%±5.5%、39.7%±2.5%、19.5%±2.5%,C组显著低于A、B组,B组显著低于A组,差异均有统计学意义(P<0.05)。前屈压缩刚度C组显著高于A、B组(P<0.05),A、B组间比较差异无统计学意义(P>0.05);后伸压缩刚度C组显著高于A组(P<0.05),但A、B组间及B、C组间差异均无统计学意义(P>0.05);左侧弯及右侧弯压缩刚度3组间比较差异均无统计学意义(P>0.05)。结论胸腰椎爆裂骨折椎弓根钉棒系统固定结合经单侧椎管伤椎椎体内植骨较经双侧椎弓根伤椎椎体内植骨植入骨量更多,更充分,术后骨缺损空隙残存率更小,对恢复脊柱前屈-压缩刚度更好。     

Confidence高黏度骨水泥椎体成形系统结合体位复位治疗急性重度骨质疏松性椎体压缩骨折

目的探讨应用Confi dence高黏度骨水泥椎体成形系统结合体位复位治疗急性重度骨质疏松性椎体压缩骨折(osteoporotic vertebral compression fracture,OVCF)的临床效果。方法回顾分析2004年6月-2009年6月采用Confi dence高黏度骨水泥及其椎体成形系统结合体位复位治疗34例急性重度OVCF患者的临床资料。男14例,女20例;年龄62～88岁,平均72.6岁。均为单椎体骨折。损伤节段:T11 4例,T12 10例,L1 15例,L2 4例,L3 1例。骨密度测定T值均≤—2.5,提示骨质疏松。伤后至入院时间2～72 h。术前先对压缩椎体行腰椎过伸位复位7～14 d,术中采用单侧穿刺,经椎弓根入路,每个椎体注射骨水泥2～6 mL,平均3.2 mL。结果术中3例(8.8%)椎体出现不同程度骨水泥渗漏,其中2例椎体渗漏至椎间隙,1例椎体渗漏至椎旁软组织;均无临床症状,未行处理。患者均无肺栓塞、感染和神经损伤等并发症发生。34例均获随访,随访时间12～38个月,平均18.5个月。术后31例术前疼痛症状完全缓解,3例部分缓解;未见伤椎再骨折、骨与骨水泥界面松动及相邻椎体骨折发生。术后3 d及末次随访时伤椎前中柱椎体高度、后凸Cobb角及疼痛视觉模拟评分(VAS)均较术前显著改善(P<0.05),术后3 d与末次随访时比较差异无统计学意义(P>0.05)。结论 Confi dence高黏度骨水泥椎体成形系统具有瞬间高黏度、可注射时间长、定向可控注射等优点,降低了骨水泥渗漏风险,术前结合体位复位治疗急性重度OVCF疗效较好。     

椎体成形术对胸腰椎爆裂型骨折的治疗意义

目的：探讨腰腰椎爆裂型骨折撑开复位与椎体成形术后椎体结构和生物力学性能的变化。方法：收集6具新鲜成人尸体的胸腰椎标本，制成T11－L1、L2－L4、T12－L2节段标本共10具，用自由落体撞击试验造成中间椎体爆裂型骨折，撑开复位、用注射型自固定磷酸钙人工骨行椎体成形术。分别于骨折前、骨折撑开复位后、椎体成形术后用薄层CT扫描测量中间椎体内空隙，用双能X线骨密度仪测定骨密度，用万能材料试验机测定骨折前、椎体成形术后中间椎体与其上方椎间盘在前屈、后伸、侧屈和扭转就压力 下刚度的变化，并比较成形术后的伤椎及其下方的完整椎体的抗极限压缩测试结果。结果：8具标本造中间椎体爆裂型骨折模型成功。（1）骨折前椎体内无明显空隙；骨折并撑开复位后椎体内空隙体积平均为5．25cm^3，占椎体总体积的13．9％；椎体成形术后空隙减少，与骨折前相比差异无显著性意义。（2）骨折前椎体骨密度在正常范围，骨折并撑开复位后骨密度较骨折前降低；椎体成形术后，骨密度较骨折复位后及骨折前均明显升高。（3）椎体成形术后，伤椎的刚度与骨折前相比差异无显著性意义，抗极限压缩强度的均值低于其下方完整椎体，但差异无显著性意义；伤椎上方椎间盘在前屈和后伸应力下的刚度小于骨折前，但在侧屈应力下差异无显著性意义；标本在扭转应力下的刚度小于骨折前。结论：（1）撑开复位未能恢复胸腰椎爆裂型骨折椎体结构上的完整性，这可能是后路切开复位内固定术后发生内固定失败与矫正度丢失的重要原因。（2）应用注射型自固化磷酸钙人工骨行椎体成形术有助于伤椎的重建，术后脊柱的生物力学特性接近骨折前水平。     

骶骨部分切除对骶髂关节生物力学的影响

目的:评价骶骨部分切除对骶髂关节生物力学的影响;明确骶骨切除范围与稳定重建的关系.方法:7具成人尸体L5-骨盆标本;分别在完整状态、S2以下切除(A组)、1/2 S2以下切除(B组)、S1以下切除(C组)、1/2 S1以下切除(D组)、右侧骶髂关节切除(E组)等情况下通过858型MTS材料实验机给标本施加800N轴向压缩和7Nm轴向扭转载荷;记录完整状态及残留骶髂关节刚度;比较各组间的差异.结果:轴向压缩时;A组～E组残留骶髂关节刚度分别是完整状态组的98.7%、97.1%、94.4%、82.9%和55.2%;完整状态组、A组、B组和C组间的压缩刚度无显著性差异;D组和E组的轴向压缩刚度显著低于完整状态组.轴向旋转时;A组～E组残留骶髂关节刚度分别为完整状态刚度的90.7%、88.5%、81.9%、71.9%和44.5%;完整状态组、A组和B组间的旋转刚度值无显著性差异;C组、D组和E组的轴向旋转刚度显著低于完整状态组和A组.结论:骶骨切除范围与骶髂关节稳定性相关.骶骨向上切除至S1将导致骶髂关节旋转失稳.切除至1/2 S1将进一步引起残留骶髂关节的轴向压缩失稳.骶骨切除超过上述范围时应考虑局部稳定性重建.     

前路单节段融合内固定治疗伴椎弓根断裂的Denis B型胸腰椎爆裂骨折生物力学研究

目的探讨前路单节段融合内固定治疗伴椎弓根断裂的Denis B型胸腰椎爆裂骨折后的脊椎生物力学稳定性。方法取6具新鲜成人尸体胸腰椎标本(T11~L3)作为正常组(A组),采用椎体切除法依次建立L1Denis B型爆裂骨折模型并行前路单节段融合内固定,分别为椎弓根完整组(B组)、单侧椎弓根切断组(C组)和双侧椎弓根切断组(D组)。通过脊柱三维运动机依次测定各组在8.0 N·m纯力偶矩下屈伸、左右侧弯及左右旋转活动度(range of motion,ROM)。结果 B、C、D组T12、L1脊柱运动单元前屈、后伸、左右侧弯ROM均显著低于A组(P0.05),D组显著高于B、C组(P0.05),B、C组间差异无统计学意义(P0.05);B、C组左右旋转ROM均显著低于A、D组(P0.05),B、C组间及A、D组间比较差异无统计学意义(P0.05)。各组间L1、L2脊柱运动单元前屈、后伸、左右侧弯、左右旋转ROM比较,差异均无统计学意义(P0.05)。结论前路单节段融合内固定治疗Denis B型胸腰椎爆裂骨折伴一侧椎弓根断裂时,在屈伸、侧弯及旋转方向能提供足够初始生物力学稳定性,而伴双侧椎弓根断裂时生物力学稳定性差。     

椎体后凸成形术中灌注不同凝固状态骨水泥对骨质疏松性椎体压缩性骨折绵羊椎体生物力学的影响

目的 探讨椎体后凸成形术中灌注不同凝固状态骨水泥对骨质疏松性椎体压缩性骨折(OVCF)绵羊椎体强度和刚度的影响.方法 选取成年绵羊8只,获得L1～5椎体40个,随机分为4组,每组10个.采用3％稀盐酸浸泡和双侧椎弓根微泵灌注法制作骨质疏松椎体模型,再将骨质疏松椎体置于衡翼生物力学机上并压缩其高度的1/4制作压缩骨折椎体模型.制备骨水泥灌注通道后,用球囊经双侧椎弓根复位骨折椎体,在C形臂X线机透视下,对照组(A组)不灌注骨水泥,其余各组分别在聚甲基丙烯酸甲酯(PMMA)骨水泥粉液混合后2 min(骨水泥稀薄期,B组)、4 min(骨水泥黏稠期,C组)、6 min(骨水泥凝固期,D组)灌注骨水泥.室温放置24 h待骨水泥凝固.分别于术前和术后测量各组椎体的强度和刚度.结果 术前4组椎体强度和刚度组间比较,差异均无统计学意义(P>0.05).A组术后椎体强度低于术前,B、C、D组术后椎体强度均高于术前,差异有统计学意义(P<0.05).4组术后椎体刚度均低于术前,差异有统计学意义(P<0.05).B、C、D组术后椎体强度和刚度均高于A组,差异有统计学意义(P<0.05);B、C组术后椎体强度和刚度均高于D组,差异有统计学意义(P<0.05);B组和C组术后椎体强度和刚度差异无统计学意义(P>0.05).结论 OVCF绵羊采用椎体后凸成形术治疗,注入稀薄期和黏稠期骨水泥的椎体强度和刚度均高于注入凝固期骨水泥的椎体.注入不同凝固状态骨水泥均可增强椎体强度,但椎体刚度均恢复不到未骨折时期状态.     

维生素C对乳腺癌细胞紫杉醇化疗的增敏作用

目的:探讨维生素C(VitC)对乳腺癌细胞紫杉醇(PTX)化疗的增敏作用及机制。方法:分别将VitC与PTX单独或联合作用原代培养的人乳腺癌细胞后,用MTS法、流式细胞仪、Westernblot检测细胞的增殖、凋亡以及caspase-3与Bcl-2的表达。结果:单独用药时,VitC对乳腺癌细胞的IC_(50)为2.5mmol/L,PTX为8.6nmol/L,但联合1mmol/L的VitC后,PTX对乳腺癌细胞的IC_(50)为2.8nmol/L;VitC单用有一定的促乳腺癌细胞凋亡作用,PTX与VitC联用后的促乳腺癌细胞凋亡作用明显大于PTX单用,且随着VitC浓度的增加而增强(均P0.05);PTX单独作用后,乳腺癌细胞caspase-3的表达上调,Bcl-2的表达下调,联合VitC后该作用更加明显,且随VitC浓度的增加而增加(均P0.05)。结论:VitC可提高乳腺癌细胞PTX化疗的敏感性,该作用可能与其进一步提高caspase-3、降低Bcl-2的表达有关。     

MTS colorimetric assay in combination with a live-dead assay for testing encapsulated L929 fibroblasts in alginate poly-L-lysine microcapsules in vitro

Biomaterials such as applied in microcapsules may have harmful effects on encapsulated cells. Up to now, there are no adequate assays available for testing the function and viability of cells in capsules. Therefore, we investigated whether the combination of MTS proliferation assay and live-dead viability assay is suitable for testing microencapsulated L929 fibroblasts in long-time culture. Proliferation of L929 cells was shown by a significant increase of formazan absorbance within the first 3 weeks (Day 0: 0.132 +/- 0.047; Day 7: 0.404 +/- 0.101; Day 14: 0.728 +/- 0.239; Day 21: 0.877 +/- 0.224) followed by stagnation and decrease thereafter. This was confirmed by an increasing proportion of dead cells measured by the live-dead assay. Thus, proliferation of encapsulated L929 can be reliably investigated by the MTS assay. In combination with life-dead assays, the proliferation can be correlated to the survival rate of the encapsulated cells.     

应用MTS方法检测生物人工肾小管的细胞活性

目的:探讨应用MTS方法在生物人工肾小管(RAD)构建过程中测定血液滤器中肾小管上皮细胞活性的可行性.方法:采用人肾近曲小管上皮细胞株(HK2)体外培养,然后按不同浓度植入6孔板,应用MTS方法测定490 nm吸光度(OD)值,判断其测定值与细胞数量之间的关系.然后将培养的HK2细胞植入FH66血液滤过器内腔继续培养构建RAD,在培养过程中每隔3 d用MTS方法测定一次OD值,并与无细胞植入的空白滤器作对照.结果:HK2细胞数在105～106范围内,与OD值呈正相关关系,相关系数(r)为0.985,P<0.01.各时间点RAD组MTS测定均值与对照组相比均有统计学意义(P<0.01,n＝5).RAD组MTS测定值随培养时间的延长而增加,提示RAD在培养过程中细胞增殖.结论:MTS方法能较好地反映RAD内的细胞活性,能在RAD培养过程中作为一种简便有效的手段监测滤器内的细胞活性.     

Staged reposition and fusion with external fixator in spondyloptosis

PROBLEM: Is the staged reduction by an external fixateur and combined fusion of slipping vertebra an adequate surgical treatment for lumbar spondyloptosis? METHODS: 11 patients with symptomatic lumbar spondyloptosis were treated using a technique of slow reduction and combined posterior and anterior approach. The first stage consists of a posterior approach with the application of the external fixateur. After staged reduction the anterior and posterior fusion are performed. All patients were pre- and postoperatively classified by radiological and clinical criteria. We report improvements in pain, activities of daily live, and cosmetic appearance. The average follow-up was over three years. RESULTS: Postoperatively one patient failed to reduce, and one developed a subsequent L4/L5 spondylolisthesis. All patients showed a solid spondylodesis with no significant loss of reduction. There was no patient with any neurological deficit. The mean correction of the slipping at follow-up was 84.5%. 9 patients were satisfied with the result. CONCLUSION: With gradual instrumented reduction by external fixateur, an anatomic reduction is nearly obtained and excellent clinical results in the treatment of spondyloptosis can be achieved.     

15-Deoxy-Delta12,14-prostaglandin J2 regulates mesangial cell proliferation and death

BACKGROUND: Proliferation of intrinsic glomerular cells is a common response to renal injury. Acutely, proliferation may be beneficial, but sustained glomerular hypercellularity after injury is associated with progressive renal failure. To identify endogenous factors that may be responsible for regulating glomerular cell number, the effects of J-series cyclopentenone prostaglandins (PGs) on human glomerular mesangial cell proliferation and death were examined. METHODS: Human mesangial cells were grown in the presence or absence of PGJ2 or its metabolite 15-Deoxy-Delta12,14-PGJ2 (15dPGJ2). The number of viable cells was measured by the reduction of the tetrazolium MTS to a colored formazan product. Apoptosis was assessed by caspase-3 activation and DNA fragmentation. RESULTS: PGJ2 at concentrations up to 10 micromol/L caused mesangial proliferation. 15dPGJ2 also caused mesangial proliferation at low concentrations (< or =2.5 micromol/L), but induced mesangial cell death at higher concentrations (>5 micromol/L). Cell death occurred in part through apoptosis, measured as an increase in caspase-3 activity and DNA fragmentation in 15dPGJ2-treated cells. Cell death was associated with a decline in baseline phosphorylation of the survival factor Akt and increased Akt degradation, whereas 15dPGJ2-induced mesangial proliferation was blocked by inhibition of the PI 3-kinase/Akt pathway. 15dPGJ2 is a potent PPARgamma agonist. Like 15dPGJ2, treatment of mesangial cells with thiazolidinedione-type PPARgamma ligands (10 to 20 micromol/L) caused significant cell death, but at lower concentrations also caused a small degree of proliferation. CONCLUSIONS: J-series prostaglandins thus may be involved in the initiation of glomerular hypercellularity through Akt-dependent proliferation, and restoration of normal glomerular architecture through PPARgamma-mediated apoptosis. Manipulation of these prostaglandins may be relevant to the treatment of progressive glomerular disease.     

Amino acid uptake and regulation in multicellular hepatoma spheroids

BACKGROUND: Cancer cells maintained in monolayer tissue culture are frequently used to study tumor biology and nutrient uptake, but there is a concern that this system may not fully reflect clinical tumor physiology. Because cells grown in a 3-D configuration more closely resemble an in vivo environment, a model was developed and characterized for the growth of SK-Hep human hepatoma cells in suspension as multicellular tumor spheroids (MTS). The measurement of nutrient uptake in such a system has never been established. MATERIALS AND METHODS: SK-Hep cultures were initiated as single cell suspensions and grown as MTS in siliconized spinner flasks. The transport of several individual amino acids (arginine, glutamate, leucine, alpha-(N-methylamino)isobutyric acid (MeAIB), and glutamine (GLN)) was measured in SK-Hep single cell suspensions and MTS (0. 50-0.60 mm diameter) by a radiotracer/rapid filtration technique, as was the regulation of glutamine uptake by phorbol esters. l-[(3)H]GLN uptake was also measured in larger spheroids (0.85-1.5 mm diameter). MTS cellularity was evaluated by histological examination, and single cell integrity after the transport assay was confirmed by scanning electron microscopy (SEM). RESULTS: SK-Hep MTS displayed gradients of cellular morphology and staining, with central necrosis visible at diameters >0.8 mm. Single cell suspensions endured the rapid filtration technique based on functional Na(+)-dependent uptake rates and SEM analysis. Of all amino acids tested, only GLN transport rates were visibly affected by growth format. In small MTS, Na(+)-dependent GLN uptake was diminished by 40%, but was 40-53% higher in MTS >1 mm displaying central necrosis, when compared to single cell suspensions. Likewise, slight parallel changes in glutamine transporter ATB(0) mRNA levels were observed in Northern blot analysis. Finally, phorbol ester-dependent GLN transport down-regulation (by 40-50%), previously established in SK-Hep monolayers, remained operative in all cell formats tested. CONCLUSIONS: The data suggest that the tumor microenvironment differentially impacts the uptake of specific nutrients despite the conservation of key regulatory pathways. This MTS technique may prove useful for further studies on the role of nutrient transport in nascent tumor growth.     

抑癌基因MTS1对人瘢痕疙瘩成纤维细胞的生物学影响

目的：探讨多肿瘤抑制基因1（MTS1）对人瘢痕疙瘩成纤维细胞在生长增殖、DNA合成代谢及胶原合成量等方面的影响。方法：将外源性MTS1基因导入人瘢痕疙瘩成纤维细胞后，通过MTT法测定细胞的生长曲线，通过^3H-胸腺嘧啶核苷（^3H-TdR）及^3H-脯氨酸掺入的方法测定细胞的DNA合成量及胶原合成量，来分别比较转染前后细胞的生物学变化。结果：在转染MTS1后瘢痕疙瘩成纤维细胞的生长增殖受到明显的抑制，同时其DNA合成量及胶原合成量也明显降低，而转染空载体组及正常组细胞均未出现上述变化。结论：外源性多肿瘤抑制基因1(MTS1）能明显抑制人瘢痕疙瘩成纤维细胞在生长增殖、DNA合成及胶原合成等方面生物学功能，为瘢痕疙瘩的临床治疗提供了新的思路。     

松口蘑糖蛋白联合5-Fu体外对人肝癌细胞Bel-7402的影响及作用机理初探

采用MTT法研究了松口蘑抗肿瘤糖蛋白MTS03与化疗药物5-Fu单独及联合使用对人肝癌细胞Bel-7402的影响及对人正常肝细胞L-02的毒性作用，观察不同浓度配比及不同作用时间对细胞是否具有增效减毒的作用；采用扫描电镜观察MTS03作用后的Bel-7402细胞形态．结果表明，MTS03与5-Fu在较低浓度单独使用对肿瘤细胞无显著差异，但两者联合使用增强了对肿瘤细胞的杀伤作用，而对正常细胞的作用较小，联合使用的q值均大于0．85，说明不存在拮抗作用．扫描电镜观察到凋亡小体的形成，随着剂量升高，凋亡小体增多．这一结果说明两者合用后MTS03增加了5-Fu对肝癌细胞的敏感性，同时降低了两者单一高剂量使用时引起的毒副作用，同时MTS03能诱导Bel-7402细胞膜皱缩和凋亡小体的形成．     

MTS1基因在人BPH中突变缺失的初步研究

目的探讨ＭＴＳ１基因在人前列腺增生症（ＢＰＨ）发病机制中的作用和变异情况。方法用ＰＣＲ银染ＳＳＣＰ技术检测１８例正常前列腺和３８例ＢＰＨ中抑癌基因ＭＴＳ１各外显子的纯合性缺失和突变。结果１２例ＢＰＨ出现ＭＴＳ１基因缺失,总缺失率为３２％;正常前列腺组织中有１例出现ＭＴＳ１基因缺失,缺失率为６％,两者之间差异有显著性（Ｐ＜００５）;无１例有ＭＴＳ１基因的突变。结论ＢＰＨ的发病机制可能与ＭＴＳ１的纯合性缺失有关,未见有突变发生。应对ＭＴＳ１基因在人ＢＰＨ中的作用作更深入的研究