类风湿关节炎患者血清IL 37和可溶性PD 1分子的表达水平及临床意义

目的：通过研究类风湿关节炎（RA）患者血清IL 37和可溶性PD 1分子的表达水平,初步探讨其与RA的相关性以及临床意义。方法：收集RA患者及对照组人群的外周血,然后采用酶联免疫吸附法（ELISA）检测30例RA患者（RA标准评分≥6）和30名健康对照组血清中IL-37、sPD-1的表达水平,结合分析两组人群外周血IL-18、IL-6和IL-18BP三种细胞因子的表达,并通过Pearson相关分析其相关性。结果：经ELISA检测发现,RA组患者外周血中IL-37和sPD-1以及其他几种炎症相关细胞因子IL-18、IL-18BP、IL-6的表达水平均高于健康对照组（P＜0.05）;且IL-37与细胞因子IL-18、IL-18BP、IL-6均呈正相关,sPD-1仅与细胞因子IL-6呈正相关,IL-37、sPD-1均与患者病情程度评分呈正相关。结论：IL-37、sPD-1在RA患者中表达水平升高,且与其他炎性细胞因子及疾病严重程度具有相关性,两者在RA的进展中可能发挥着重要的调控作用,为今后RA治疗提供新的依据。     

内脂素在妊娠期糖尿病中的表达及意义

目的探讨内脂素,胰岛素与妊娠期糖尿病的关系。方法采用酶联免疫吸附实验法检测50例妊娠期糖尿病患者（研究组）,和30例正常妊娠孕妇（对照组）孕24-36周外周血中内脂素胰岛素表达水平。结果随孕周增加,血清内脂素和胰岛素呈逐渐增加趋势,相同时间段内研究组血清内脂素,胰岛素的表达均显著高于对照组,（χ2visfatin=5.00,P〈0.05;χ2insulinum=5.03,P〈0.05）,且两者呈正相关,结论内脂素可能通过影响糖脂代谢,参与胰岛素抵抗,在妊娠期糖尿病发病中可能有重要作用。     

同型半胱氨酸浓度变化与妊娠高血压综合征患者的关系

目的探讨妊娠晚期孕妇血清同型半胱氨酸（Hcy）和叶酸（FA）水平与妊娠高血压的相关性。方法采用化学发光法及荧光标记免疫法检测30例妊高征妇女和30例正常孕妇女（对照组）血清Hcy与FA含量。结果正常妊娠组血清Hcy和FA水平与妊娠期高血压组比较,差异有统计学意义（P〈0.05）。结论测定妊娠期孕妇血清FA、Hcy水平对指导孕妇合理饮食,减少和预防妊娠高血压的发生有重要的意义。     

IL-18、IL-10和IL-6与急性冠状动脉综合征的关系研究

目的：研究白细胞介素（IL）-18、IL-10和IL-6血清浓度与急性冠状动脉综合征之间的关系。方法： 采用酶联免疫吸附法（ELISA）和放射免疫法检测62例冠心病患者（急性心肌梗死17例、不稳定性心绞痛30例、稳定性心绞痛15例）和20例正常健康者血清IL-18、IL-10和IL-6水平，并比较上述指标水平之间的相关关系。 结果： 血清IL-18、IL-6水平在急性心肌梗死（AMI）组和不稳定性心绞痛（UAP）组显著高于稳定性心绞痛（SAP）组和对照组(P＜0.05, P＜0.01)；AMI组和UAP组血清IL-10水平明显低于SAP组和对照组（P＜0.01）。血清IL-18和IL-6水平与血清IL-10水平呈显著负相关（r＝－0.827, P＜0.01; r＝－0.231, P＜0.05)； 血清IL-6水平与IL-18水平相关性不明显（r＝0.119, P＞0.05）。 结论： 急性冠状动脉综合征患者血清IL-18、IL-6水平明显升高而IL-10水平显著降低；IL-18、IL-6与IL-10的平衡失调可能是促进斑块不稳定的重要因素。     

正常妊娠妇女血清VB_(12)、FA、SF、CG指标分析

妊娠期间 ,由于胎儿的生长和发育、孕妇体内发生一系列生理变化 ,确认这些生理变化 ,对妊娠期间疾病的诊断、减少妊娠并发症、保证胎儿的正常体格发育和智力发育具有重要意义。本文观察了正常妊娠妇女在不同孕期血清中维生素Ｂ１２（ＶＢ１２）、叶酸（ＦＡ）、血清铁蛋白（ＳＦ）、血清甘胆酸（ＣＧ）水平的变化 ,并进行了分析。对象和方法一、对象 :１０５例正常孕妇。孕前及孕期无肝肾疾病、血液病 ,其中早孕组（５～１２周）３５例 ,中孕组（１３～２８周）３２例 ,晚孕期（２８～３６周）３８例 ,年龄２２～３０岁。正常未孕对照组５０…     

糖皮质激素升高因子在自身免疫性甲状腺疾病发病中的作用

目的：观察外周血中糖皮质激素升高因子（GIFs）水平在GIF-HPA反馈回路及实验性自身免疫性甲状腺炎（EAT）形成中的作用,探讨AITD发病机理。方法：采用EAT易感的Lewis大鼠和非易感的Wistar大鼠,均用Tg免疫诱发EAT形成,观察免疫后不同时间（初次抗原免疫后3～4小时和8～12小时及EAT形成时）,其甲状腺组织炎症反应和血清中皮质醇、GIFs（IL-1β,IL-6）和TPOAb水平。结果：诱发EAT的Lewis大鼠甲状腺炎发病率及甲状腺炎细胞浸润程度,均明显重于Wistar大鼠。Tg免疫后3～4小时,Lewis大鼠血清中皮质醇水平呈轻度升高,而Wistar大鼠明显升高。两种大鼠血清中GIFs（IL-1β,IL-6）水平动态变化与各自皮质醇水平的变化类似,免疫后Wistar大鼠血清中两种细胞因子水平的升高水平均明显高于Lewis大鼠。免疫后（3～4小时组除外）Lewis大鼠血清中TPOAb水平明显高于Wistar大鼠。结论：可以认为个体免疫应答生成的IL-1β、IL-6等细胞因子水平会影响GIF-HPA反馈回路,其异常反应也是构成AITD发病的因素。     

动态观察131I治疗对Graves病患者血清细胞因子水平的影响

目的：探讨^131I治疗对Graves病（GD）患者血清细胞因子水平的影响。方法：用放射免疫分析法检测52例GD患者^131I治疗前及治疗后30、90和180天血清IL-1、IL-2、IL-6、IL-8、TNF-α水平，并与血清甲状腺激素进行相关性分析，30例年龄与性别相匹配的健康者作为正常对照。结果：GD患者血清IL-1、IL-6、IL-8、TNF-α水平显著高于对照组（P〈0．01），IL-2显著低于对照组（P〈0．01）；经^131I治疗，甲状腺功能恢复正常后，IL-1、IL-8水平明显降低，IL-2明显升高，与对照组比较差异无显著性（P〉0．05）；IL-6、TNF-α明显低于治疗前（P〈0．01），但仍高于对照组（P〈0．05）。血清IL-1、IL-6与FIB呈正相关（P〈0．05），IL-2与FIB呈负相关（P〈0．05）。结论：^131I治疗能有效抑制GD患者自身免疫，其治疗前后血清细胞因子的变化可反映GD的免疫动态，可能与发病机制、治疗效果和预后有关。     

孕酮及其受体、IL-8与原因不明自然流产的相关性研究

目的：探讨孕酮（P）及其受体（PR）、IL-8在原因不明自然流产患者外周血及母胎界面的表达及相关性。方法：ELISA检测30例原因不明自然流产患者（流产组）和30例正常妊娠妇女（对照组）血清P、IL-8水平,免疫组织化学染色法检测两组流产绒毛和蜕膜组织PR、IL-8表达。结果：流产组血清P水平明显低于对照组（P<0.01）,血清IL-8水平明显高于对照组（P<0.01）。两组绒毛组织PR蛋白表达均为阴性;两组蜕膜组织中PR均表达于蜕膜细胞的细胞核,且流产组表达明显低于对照组（P<0.01）。两组绒毛组织中IL-8均表达于绒毛上皮细胞的细胞质,且流产组表达明显高于对照组（P<0.01）;两组蜕膜组织中IL-8均表达于蜕膜细胞的细胞质,且流产组表达高于对照组（P<0.05）。流产组血清P与IL-8呈显著负相关（r=-0.870,P<0.01）,流产组蜕膜组织PR与IL-8呈显著负相关（r=-0.650,P<0.01）。结论：P、PR低表达、IL-8高表达可能与原因不明自然流产发生相关,且P、PR可能通过下调IL-8表达维持正常妊娠。     

妊高征患者血浆血管内皮活性因子及相关白细胞介素水平的变化

目的：探讨妊高征患者血浆ET、NO和血清IL-6、IL-8水平的变化与患者发病的关系。方法：35例非孕妇女、35例正常孕妇（对照组）及32例妊高征患者的血浆ET、血清IL-6及IL-8含量均采用放射免疫分析;血浆NO采用生化比色法。结果：35名正常孕妇血浆ET、NO均显著高于正常非孕妇女组（P〈0.01）;IL-6和IL-8水平则略有升高,但尚无统计学意义（P〉0.05）。32例妊高征患者治疗前血清ET、IL-6及IL-8三项指标水平较对照组升高极显著（P〈0.01）;NO水平则显著低于对照组（P〈0.01）。经治疗2周,ET、IL-6及IL-8三项指标较对照组下降均非常显著,与对照组比较已无显著差异（P〉0.05）;而NO水平则升高显著,与对照组比较亦无显著差异（P〉0.05）。相关分析结果表明,血浆ET水平与IL-6及IL-8水平呈显著正相关（r=0.6097、0.7213,P〈均0.01）。结论：妊高征患者血清4项指标的测定对于了解和认识其发病机理及预估病情有帮助。     

细胞因子IL-6及TNF-α在妊娠期高血压风险预测价值中的研究

目的探讨联合检测孕妇外周血中细胞因子(cytokines)白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的表达水平对妊娠期高血压发病风险的预测价值。方法选择妊娠期高血压患者30例(子痫前期患者24例)为妊娠期高血压组,同期正常中、晚孕产检孕妇36例为对照组。采用流式荧光法检测两组孕妇外周血清中IL-2、IL-4、IL-6、IL-10、TNF-α和γ干扰素(IFN-γ)的表达水平,分析各细胞因子在两组患者中表达水平的差异及意义,并采用多因素Logistic回归方法分析各细胞因子表达水平与妊娠期高血压及子痫前期发病风险的相关性。结果妊娠期高血压组患者外周血中IL-6、TNF-α水平分别为18.29±19.47 pg/mL、9.92±11.04 pg/mL,正常组孕妇外周血中IL-6、TNF-α的表达水平分别为4.91±0.73pg/mL、4.28±0.51pg/mL,经统计学分析,两组IL-6、TNF-α水平相比较,差异均有显著性意义(P0.05)。而两组其它细胞因子水平相比较则均无显著性差异(P0.05)。采用多因素logistic回归分析显示细胞因子IL-6、TNF-α表达水平增高均是影响妊娠期高血压发生风险的独立因素。同时,依据上述分析结果,建立了在中、晚孕期联合检测孕妇外周血中细胞因子IL-6、TNF-α水平来预测妊娠期高血压发生概率的模型。结论孕、中晚期联合检测细胞因子IL-6、TNF-α表达水平在妊娠期高血压风险预测中具有较高价值。     

Progesterone-Induced Blocking Factor and Cytokine Profile in Women with Threatened Pre-Term Delivery

Problem  The objective of this study was to compare serum concentrations of progesterone-induced blocking factor (PIBF), anti-inflammatory (IL-10), and pro-inflammatory (IL-6, TNFα, and IFNγ) cytokines of women with threatened pre-term delivery, with those of women with normal pregnancy and to evaluate the impact of PIBF on the outcome of pregnancy.
Method of study  A prospective study was conducted on a sample of 30 women with threatened pre-term delivery (study group) and 20 healthy pregnant women (control group) between the 24th and 37th gestational weeks. Serum PIBF, anti-inflammatory (IL-10), and pro-inflammatory (IL-6, TNFα, and IFNγ) cytokine concentrations were measured by enzyme-linked immunosorbent assay (ELISA).
Results  Thirteen of 30 patients (43.3%) with symptoms of threatened pre-term delivery, and one of 20 patients (5%) in the control group delivered before the 37th week of gestation. Mean PIBF concentrations in serum samples of patients with threatened pre-term delivery were significantly lower than in those of healthy pregnant women (171.12 ± 162.06 ng/mL versus 272.85 ± 114.87 ng/mL; P  < 0.05). Women with symptoms of threatened pre-term delivery had significantly lower serum levels of IL-10, and higher levels of IL-6 as well as IFNγ compared with healthy controls.
Conclusion  Our results indicate that measuring PIBF and cytokine concentrations in serum during pregnancy is feasible and may be important for understanding immunological causes of pre-term delivery.     

NLR、CRP及IL-6、IL-17在子宫内膜异位症中的表达及与病情的相关性

目的探讨NLR(中性粒细胞与淋巴细胞比值)、CRP(C反应蛋白)、IL-6(白细胞介素6)、IL-17(白细胞介素17)在子宫内膜异位症(EMs)中的表达水平及其与病情的相关性。方法回顾性分析2017年7月至2019年7月在唐山市妇幼保健院妇科行腹腔镜手术的126例术后病理确诊为EMs的患者作为研究对象,其中Ⅰ~Ⅱ期为轻度组(73例),Ⅲ~Ⅳ期为重度组(53例),选取同期体检的健康妇女50例作为对照组。分别测定各组CRP、IL-6、IL-17及NLR指标的水平,分析各指标的关系并应用Logistic回归分析各指标与EMs严重程度的相关性。结果EMs组患者血清中的CRP、IL-6、IL-17和NLR水平均高于对照组,IL-6分别与IL-17和CRP成正相关(r=0.348,P<0.05;r=0.308,P<0.05),CRP与NLR成正相关(r=0.297,P<0.05)其他指标间无显著相关性。重度组CRP、IL-6、IL-17水平均高于轻度组,差异有统计学意义(P<0.05)NLR重度组与轻度组差异无统计学意义(P>0.05)。Logistic回归分析显示,CRP、IL-6、IL-17与EMs病情均密切相关(P<0.05),三者联合诊断EMs的价值最高。结论CRP、IL-6、IL-17和NLR参与了EMs的发生、发展,三个指标的检测可评估EMs的病情及预后判断,CRP、IL-6、IL-17三个指标联合检测可提高EMs诊断的灵敏度和特异性。     

Changes in serum cytokine concentrations during the menopausal transition

OBJECTIVE: The purpose of the present study was to clarify the changes in serum concentrations of 17 cytokines in healthy women during the menopausal transition by using a multiplexed cytokine assay and to clarify the associations of these cytokines with serum estradiol concentration. METHODS: Sixteen premenopausal, 54 perimenopausal and 52 postmenopausal women were enrolled in this study. Seventeen cytokines in serum samples were measured simultaneously using a Bio-Plex human cytokine 17-Plex assay. RESULTS: Serum IL-6 concentration showed a weak positive correlation with age (r=0.196, p<0.05). Postmenopausal women for whom less than 5 years had passed since menopause showed significant (p<0.05) increase in serum concentrations of IL-2, GM-CSF and G-CSF, while serum IL-4 concentration was significantly (p<0.05) increased in postmenopausal women for whom more than 5 years had passed since menopause. Serum estradiol concentration showed a significant negative correlation with serum IL-6 concentration and weak negative correlations with serum concentrations of IL-2, IL-8 and GM-CSF. CONCLUSION: We were able to simultaneously measure the levels of 17 cytokines using a highly sensitive cytokine assay, and we found that the changes in serum cytokine concentrations during the menopausal transition differed. We also found that serum IL-6 concentration during the menopausal transition was negatively correlated with serum estradiol concentration.     

大骨节病患者血清促炎症细胞因子水平的检测

目的探讨前炎症细胞因子TNF、IL-1β和IL-6在大骨节病(KBD)发病机制中的作用。方法采集62例KBD患者和60例健康对照的血清标本,采用双抗体夹心ELISA法测定血清前炎症细胞因子TNF、IL-1β和IL-6的水平。结果KBD患者血清IL-1β和IL-6的水平分别为(238.4±698.5)ng/L和(164.4±661.4)ng/L,健康人分别为(74.5±130.0)ng/L和(52.2±154.6)ng/L,但它们之间差异均不显著(P>0.05)。然而,KBD患者血清TNF的水平[(109.2±145.3)ng/L]高于健康对照[(40.9±89.7)ng/L],差异非常显著(P<0.01)。患者血清TNF与IL-1β的水平及血清TNF与IL-6水平的相关性均不显著(r值分别为0.0387和0.2135,P>0.05)。血清IL-1β与IL-6的水平呈显著的正相关(r=0.3460,P<0.01)。结论血清前炎症细胞因子水平的升高,可能与大骨节病的发病有关。     

Effects of estrogen versus estrogen and progesterone on cortisol and interleukin-6

Objectives

The purpose of this study was to compare the effects of 3 months of estrogen replacement therapy, estrogen plus progesterone replacement therapy and a placebo, on the resting cortisol and interleukin-6 (IL-6) levels in post-menopausal women.

Methods

Forty-three women were randomised to one of three treatment arms: estradiol 2 mg/day (ERT), estradiol 2 mg/day plus medroxyprogesterone acetate 5 mg/day (HRT), or a placebo that was administered orally for 3 months.

Results

Cortisol levels showed a significant condition by intervention interaction. Post hoc tests showed that ERT significantly increased cortisol levels after treatment compared to baseline, while in the HRT group a trend toward increased cortisol was found. No changes were observed in IL-6 levels.

Conclusions

Estrogen administration elevated cortisol levels, but this effect may be moderated by progestins. IL-6 was not altered by ERT or HRT, future studies should consider the interaction of cortisol increases on change in IL-6 expression.     

白细胞介素—6,雌激素与绝经后骨质疏松关系的研究

为探讨妇女白细胞介素－６（ＩＬ－６）及雌激素水平的变化对骨丢失的影响,本文采用四甲基偶氮唑盐微量酶反应比色法（ＭＴＴ）检测青年妇女,围绝经妇女及绝经后妇女的血清ＩＬ－６活性,用放免法检测血清雌二醇（Ｅ２）水平,双能Ｘ线吸收仪测定Ｌ２－４的骨密度。     

PCT和IL-6在感染性疾病中的诊断价值

目的：探讨血清降钙素原（PCT）与白介素-6（IL-6）在感染性疾病中的诊断价值。方法：采用罗氏全自动电化学发光免疫分析仪检测90例细菌感染性疾病、80例病毒感染性疾病和80例健康体检正常人血清PCT、IL-6含量,并对其结果进行分析。结果：细菌感染组PCT和IL-6含量显著高于病毒组及正常对照组,有显著性差异（P〈0.05）,病毒感染组PCT和IL-6含量与正常对照组比较无显著性差异（P〉0.05）,细菌感染组PCT和IL-6含量之间存在正相关性（P〈0.05）,病毒感染组PCT与IL-6含量之间无相关性（P〉0 05）,细菌感染组PCT、IL-6的阳性率显著高于病毒感染组及正常对照组,有显著性差异（P〈0.05）,病毒感染组PCT、IL-6阳性率与正常对照组相比无显著性差异（P〉0.05）,细菌感染组PCT的阳性率明显高于IL-6,有显著性差异（P〈0.05）。结论：IL-6在细菌感染中会出现阳性,但其不如PCT敏感,PCT检测是鉴别诊断细菌、病毒感染性疾病的良好指标。     

Influence of the sample collection method on salivary interleukin–6 levels in resting and post-exercise conditions

Previous studies demonstrated that no significant relationships exist between salivary and serum IL-6 in resting conditions and following exercise and that appropriate saliva collection procedures allow to avoid analytical drawbacks. This investigation aimed to: (a) compare the effects of two methods of saliva collection on IL-6 assay; (b) search for correlation between salivary and serum IL-6 in resting and post-exercise conditions; (c) evaluate the IL-6 response to isometric contractions. Seventeen sedentary subjects and fifteen athletes underwent one blood and two salivary draws: saliva was collected chewing on cotton salivettes and using a plastic straw (SA method and ST method, respectively). Afterwards, the athletes only completed a fatiguing isometric exercise of the knee extensors and blood and saliva were sampled after the exercise. In the entire group (n = 32), ST method produced higher IL-6 levels than SA method and serum sampling. The exercise elicited significant responses of lactate, serum IL-6, salivary IL-6 (by ST method): salivary IL-6 values using the ST collection method were higher at each sampling point than with the SA method. The correlation analyses applied to both resting levels in the entire group and absolute changes above baseline in the athlete group showed that: (1) no significant relationships exist between serum and salivary IL-6 levels; (2) the greater the salivary IL-6 measurement, the higher the resultant inaccuracy of the SA method; (3) significant correlations exist between isometric force and mechanical fatigue during exercise and peaks of lactate and serum IL-6. These data provided demonstration of a cotton-interference effect for the results of salivary IL-6 assay and confirmed the lack of significant correlation between salivary and serum IL-6 in resting and post-exercise conditions.     

慢性牙周炎伴肥胖患者血清中IL-6、IL-18表达水平的研究

目的 探究白细胞介素6（IL-6）、白细胞介素18（IL-18）在肥胖慢性牙周炎患者血清中的表达水平变化及意义。方法 选取2017年9月~2018年5月在佳木斯大学附属口腔医院牙周科门诊确诊为慢性牙周炎患者15例设为NP组,另选取同期慢性牙周炎伴肥胖患者15例设为FP组。收集两组患者外周血样本并记录牙周PD、CAL,采用双抗体夹心酶联免疫吸附法（ELISA法)测定样本中的IL-6、IL-18的表达水平,分析两组间指标的差异及相关性。结果 FP组血清中IL-6、IL-18分别为（9.16±2.35）pg/ml、（325.22±98.67）pg/ml,其表达水平分别高于NP组的（6.15±2.06）pg/ml和（241.52±78.82）pg/ml,差异有统计学意义（P＜0.05）;FP组中PD与血清中IL-6、IL-18呈高度正相关（r＝0.894,P＜0.01;r＝0.819,P＜0.01）;CAL与血清中IL-6、IL-18呈正相关（r＝0.885,P＜0.01,r＝0.828,P＜0.01）;NP组中PD与血清中IL-6、IL-18也有正相关关系（r＝0.842,P＜0.01,r＝0.728,P＜0.01）;CAL与血清中IL-6、IL-18呈正相关（r＝0.884,P＜0.01,r＝0.707,P＜0.01）。结论 肥胖状态所导致的外周血中IL-6、IL-18水平的升高与CP的发生发展存在着密切联系。