利胆排毒口服液抗兔内毒素血症作用的研究

目的 观察利胆排毒口服液对家兔内毒素血症的解热作用.方法 用大肠杆菌内毒素静脉注射制备家兔内毒素血症模型,观察利胆排毒口服液对模型动物体温变化的影响;用定量检测微量内毒素鲎试验法检测血浆内毒素(LPS)含量.ELISA法检测肿瘤坏死因子(TNF-α)含量.结果 3h体温反应高度及5h体温反应指数在各组间有明显的差异;兔灌服利胆排毒口服液后,可减少血浆LPS含量,降低血浆TNF-α水平.结论 利胆排毒口服液对兔内毒素血症有对抗作用.     

法尼醇X受体在胆汁淤积幼龄大鼠模型中的表达及利胆合剂的干预作用

目的研究法尼醇X受体(FXR)在胆汁淤积幼龄大鼠模型中表达及利胆合剂干预作用。方法将30只Sprague-Dawley大鼠随机分为正常组、模型组、熊去氧胆酸(UDCA)组、利胆合剂高、低剂量组。正常和模型组羧甲基纤维素钠溶液灌胃,其余UDCA或利胆合剂灌胃。第5天(正常组除外)给予α-异硫氰酸奈酯(ANIT),ANIT 48h处死。检测血清TBIL、DBIL、TBA、ALT、AST、GGT、ALP,实时荧光定量PCR检测肝组织FXR及小异源二聚体伴侣(SHP)、UDP葡糖醛酸基转移酶家族2多肽B4(UGT2B4)、胆盐输出泵(BSEP)mRNA;Western blot检测肝组织FXR及SHP、UGT2B4、BSEP蛋白。结果模型组TBIL、DBIL、TBA、ALT、AST、ALP、GGT水平较正常组增高(P0.01);与模型组比较,UDCA组、利胆合剂高、低剂量组TBIL、DBIL、TBA、ALT、AST、ALP、GGT水平降低(P0.01);与UDCA组比较,利胆合剂高、低剂量组TBIL、DBIL、TBA、ALT、AST、ALP、GGT水平降低(P0.01);与利胆合剂低剂量组比,高剂量组AST、TBA、GGT水平降低(P0.01)。模型组FXR、SHP、UGT2B4、BSEP mRNA及蛋白表达量较正常组降低(P0.01);与模型组比较,UDCA组、利胆合剂高、低剂量组FXR、SHP、UGT2B4、BSEP mRNA及高剂量组蛋白表达量升高(P0.01),低剂量组FXR、BSEP蛋白表达量升高(P0.05);与UDCA组比较,利胆合剂高、低剂量组FXR、SHP、UGT2B4、BSEP mRNA及蛋白表达量升高(P0.01,P0.05);与利胆合剂低剂量组相比,高剂量组FXR、SHP、UGT2B4、 BSEP mRNA表达量升高(P0.01,P0.05), SHP、UGT2B4蛋白表达量升高(P0.01)。结论利胆合剂可缓解ANIT诱导的胆汁淤积大鼠,且在一定范围内,剂量越高,效果越明显,其疗效优于UDCA,其机制可能与促进FXR及下游的SHP、UGT2B4、BSEP分子表达有关。     

金丹王合剂治疗急性胆囊炎40例

目的:观察疏肝利胆、清热利湿、活血化瘀类中药配伍治疗急性胆囊炎的临床疗效。方法:80例急性胆囊炎随机分为金丹王合剂组与消炎利胆片对照组,依据临床疗效标准及B超等检查,对治疗2个疗程2组进行对比分析。结果:金丹王合剂治疗急性胆囊炎的治愈率达45%、总有效率达95%,与对照组比较P<0.05。提示:金丹王合剂具有疏肝利胆、清热利湿、活血化瘀之功效。     

八正合剂抗感染作用的实验研究

目的　探讨八正合剂治疗泌尿系统感染性疾病的药理学作用基础。方法　将大肠杆菌注入小鼠膀胱后观察药物对细菌上行感染肾脏的抑制作用 ;家兔 iv大肠杆菌内毒素后观察药物对细菌内毒素引起机体发热的影响 ;采用大鼠足跖 sc角叉菜胶所致炎性肿胀模型观察药物对炎性肿胀及机械压迫足跖疼痛的抑制作用。结果　八正合剂 ig给药可显著降低大肠杆菌上行感染肾脏的带菌剖面百分率 [ED50 为 (11.0 1± 1.6 3) g/ kg,95 %可信限为9.5 0～ 12 .76 g/ kg],抑制大肠杆菌内毒素诱发家兔体温升高 ,减轻角叉菜胶所致大鼠足跖炎性肿胀及致炎后肿胀部位机械压迫痛阈降低程度。结论　八正合剂治疗泌尿系统感染性疾病的作用机制主要与其清除尿路细菌和减轻发热、肿胀、疼痛等炎症反应症状有关。     

利胆排毒口服液体外抗内毒素作用的实验研究

利胆排毒口服液是我们结合国内外研究成果,自主研制的以茵陈蒿汤与大承气汤合方的加减方。动物实验表明,其能明显降低内毒素血症小鼠死亡率,明显降低内毒素血症兔体温、血浆内毒素含量及肿瘤坏死因子水平等作用,为进一步探讨其作用机制、验证其疗效,我们对其进行了体外抗内毒素的实验研究。1材料1.1药物与试剂利胆排毒口服液自制(含茵陈、栀子、大黄、甘草、芒硝等药味),每1 mL含生药量1 g。全部药材均购自湖北省中医院,并经过湖北中医学院陈科力教授鉴定。冻干大肠杆菌内毒素工作标准品(北京生物制品检验所,150EU/支,批号2004-11);鲎试剂…     

前列宁合剂治疗细菌性前列腺炎的实验研究

目的：探讨前列宁合剂治疗细茵性前列腺炎的机理。方法：以大肠杆菌内毒素制备大鼠细菌性前列腺炎模型，观察前列宁合剂的治疗作用。结果：前列宁合荆对细菌性前列腺炎有显著的治疗作用，可使炎症大鼠的体重增加，炎症导致的前列腺粘连、质地变硬和组织病理学改变得到改善。结论：前列宁合剂对细菌性前列腺炎确有疗效。     

β-环糊精选择性提取虎杖化学成分及体外抗内毒素活性研究

目的研究β-环糊精选择性提取虎杖化学成分及其体外抗内毒素活性。方法利用水溶液中β-环糊精与客体分子包合结晶法,选择提取虎杖中的化学成分;LOPS法考察包合提取部位抗内毒素活性。结果β-环糊精选择性分离出虎杖中的化学成分并形成了包合物晶体．差热扫描图谱确证了包合物的形成;该包合物的体外抗内毒素作用强于阳性对照多粘菌素B。结论β-环糊精选择性提取部位活性明确,无需分离可直接用于制剂。     

利胆排毒口服液兔含药血清药理学研究

目的研究含药血清对内毒素的体外灭活作用。方法给兔灌服利胆排毒口服液后,采取血清进行血清药理研究。结果与正常对照组比较,用药组灭活内毒素作用明显增强。结论利胆排毒口服液能增强机体对内毒素的直接灭活作用。     

复方大青叶合剂主要药效学研究

目的:对复方大青叶合剂进行了与临床主治功能有关的主要药效学研究.方法:解热试验、抗炎试验和抗菌试验.结果:复方大青叶合剂能明显降低因大肠杆菌细菌内毒素引起的家兔体温升高;对腹腔注射金黄色葡萄球菌、流感杆菌引起的小鼠死亡有一定的保护作用;体外抗菌试验表明对金黄色葡萄球菌、流感杆菌、肺炎双球菌、链球菌(甲、乙型)均有较好的抑菌作用.能明显抑制角叉菜胶所致的大鼠足肿胀和醋酸所致的小鼠腹腔毛细血管通透性增加.结论:复方大青叶合剂具有解热、抗菌、抗炎作用.     

消炎利胆排石合剂的制备与临床应用

目的:研究消炎利胆排石合剂的制备方法及质量控制并观察该制剂对胆道结合、胆道感染、胆囊炎患者的治疗效果。方法:采用水提浓缩方法制备消炎利胆排石合剂,从制剂的性状、PH值、相对浓度、鉴别、微生物限度和稳定性考察等方面进行等质量控制。结果:建立了合剂的制备方法和质量标准,其工艺可行,治疗胆道结合、胆道感染及胆囊炎效果良好。结论:消炎利胆排石合剂组方合理、制作简单、性质稳定、疗效确切,值得推广应用。     

补体抑制活性物质鱼腥草总多糖中内毒素的去除

为建立补体抑制活性鱼腥草总多糖中内毒素的去除方法,将琼脂糖疏水色谱及聚丙烯酰胺凝胶过滤色谱分别与多粘菌素亲和吸附色谱联用纯化鱼腥草多糖。以显色基质比色法检测纯化前后内毒素的含量变化,细胞溶血法检测纯化前后体外抗补体活性变化。结果发现,疏水色谱与亲和吸附色谱的联用对内毒素的清除效果优于凝胶过滤色谱与亲和吸附色谱的联用,其清除率达42.85%。内毒素清除前后,抗补体活性没有明显变化。表明疏水色谱与亲和吸附色谱联用可用于清除鱼腥草总多糖中的内毒素且不影响其抗补体活性。     

Nerolidol Protects Against LPS‐induced Acute Kidney Injury via Inhibiting TLR4/NF‐κB Signaling

Acute kidney injury (AKI) is a critical care syndrome, resulting in acute reduction of renal function and up to 22% mortality of hospitalized patients. Nerolidol is a major component in several essential oils that possesses various pharmacological properties. The present study aimed to investigate the potential effect of nerolidol on lipopolysaccharide (LPS)‐induced AKI. Nerolidol dose‐dependently reduced the pathological injuries of kidney induced by LPS in rats. Nerolidol significantly decreased the levels of blood urea nitrogen and creatinine in LPS‐treated rats in a dose‐dependent manner. In addition, nerolidol inhibited LPS‐induced decrease of cell viability in NRK‐52E rat proximal tubular cells, which effect was concentration dependent. Nerolidol notably inhibited the increase of TNFα and IL‐1β in LPS‐treated rats and the mRNA expression of TNFα and IL‐1β in LPS‐treated NRK‐52E cells. Nerolidol suppressed the increase of toll‐like receptor 4 (TLR4) expression, phosphorylation and nuclear translocation of p65 NF‐κB in kidneys of LPS‐treated rats and LPS‐treated NRK‐52E cells. Overexpression of TLR4 and p65 NF‐κB significantly suppressed nerolidol‐induced inhibition of TNFα and IL‐1β expression and increase of cell viability in LPS‐treated cells. In summary, we found that nerolidol played a critical anti‐inflammatory effects through inhibition of TLR4/NF‐κB signaling and protected against LPS‐induced AKI. Copyright © 2017 John Wiley & Sons, Ltd.     

Praeruptorin a inhibits lipopolysaccharide‐induced inflammatory response in murine macrophages through inhibition of NF‐κB pathway activation

Praeruptorin A (PA) is a pyranocoumarin compound isolated from the dried root of Peucedanum praeruptorum Dunn (Umbelliferae). However, the antiinflammatory effect of PA has not been reported. The present study investigated the antiinflammatory effect of PA in lipopolysaccharide (LPS)‐stimulated RAW 264.7 macrophage cells. PA significantly inhibited the LPS‐induced production of nitric oxide (NO), interleukin‐1β (IL‐1β) and tumor necrosis factor‐α (TNF‐α). The mRNA and protein expressions of inducible nitric oxide synthase (iNOS), IL‐1β and TNF‐α were also suppressed by this compound. Further study showed that PA decreased the cytoplasmic loss of inhibitor κB‐α (IκB‐α) protein and inhibited the translocation of NF‐κB from cytoplasm to nucleus. Taken together, the results suggest that PA may exert antiinflammatory effects in vitro in LPS‐stimulated RAW 264.7 macrophages through inhibition of NF‐κB signal pathway activation. Copyright © 2010 John Wiley & Sons, Ltd.     

紫芝发酵菌体中三萜类化合物的抑菌活性研究

目的检测紫芝发酵菌体中三萜类化合物对大肠杆菌、金黄色葡萄球菌以及枯草芽孢杆菌的体外抑菌作用。方法杯碟法。结果紫芝三萜组分对大肠杆菌和金黄色葡萄球菌的生长有较明显的抑制作用,而对枯草芽孢杆菌的抑制作用相对较弱;该三萜对大肠杆菌和金黄色葡萄球菌的最小抑菌浓度为30 mg/ml;而对枯草芽孢杆菌的最小抑菌浓度为60mg/ml。此外,该三萜的抑菌成分在40℃和60℃下(处理2 h)较稳定,但在80℃和100℃温度下,热稳定性较差。结论紫芝三萜类化合物对大肠杆菌和金黄色葡萄球菌有明显的抑制作用。     

基于高效液相色谱-四级杆飞行时间质谱的硫酸多黏菌素E组分的结构分析

目的 建立基于高效液相色谱-四级杆飞行时间质谱(HPLC-Q/TOF-MS)的分析方法,对硫酸多黏菌素E 中的组分进行结构解析,结合光化学反应鉴定双键化组分.方法 通过HPLC-Q/TOF-MS/MS对主要组分多黏菌素E1 和E2质谱数据的分析,总结了多黏菌素类化合物结构解析的规律,采用精确分子质量、特征碎片离子确定组...     

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??OBJECTIVE To investigate the synergetic antibacterial effects of chitosan-modified polymyxin B liposomes(c-PMB-Lip) combined with ultrasound microbubbles on biofilm-producing Acinetobacter baumannii(AB) in vitro. METHODS c-PMB-Lip was prepared by an injection method. The minimum biofilm inhibition concentrations (MBIC) of the AB clinical isolates treated with different preparations of polymyxin B were determined. The antibacterial effects and the dose-effect relationship of chitosan-modified polymyxin B liposome group (c-PMB-Lip), ultrasound microbubble + polymyxin B group (USMB+PMB) and ultrasound microbubble + chitosan modified polymyxin B liposome group (USMB+c-PMB-Lip) were further revealed. RESULTS The MBIC of c-PMB-Lip was (8.0??2.0)??g??mL^-1 against biofilm-producing AB. USMB could enhance the inhibitory effects of polymyxin B on biofilm-producing AB. Moreover, USMB combined with lipid agents could obtain the most significant inhibitory effects and even completely remove the biofilm-producing AB. With the drug concentration increasing, the antibacterial effects of each group showed a dose-effect relationship within a certain range. Compared with the sterile blank group, USMB+c-PMB-Lip could completely remove the bacterial biofilm and achieve a maximum antibacterial effects at 2 ??g??mL^-1(P>0.05). CONCLUSION c-PMB-Lip combined with USMB have significant synergistic antibacterial effect on biofilm-producing AB.     

承气方剂预防大鼠肝移植后肝脏缺血/再灌注损伤的实验研究

目的：观察承气方剂预防大鼠肝移植后肝缺血／再灌注损伤的作用。方法：采用大鼠原位肝移植模型，随机分组，即中药组、多粘菌素组、甘露醇组、生理盐水组和假手术对照组，每组又分移植后1、4h组；对移植鼠血清ALT、肝损伤指数进行测定。结果：在预防肝移植后肝脏缺血／再灌注损伤中，承气方剂组和多粘菌素组优于甘露醇组和生理盐水组，承气方剂组又优于多粘菌素组。结论：承气方剂能有效减轻植后肝脏缺血／再灌注损伤。     

赤芝菌体中三萜抑菌作用研究

目的检测赤芝发酵菌体中三萜对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌以及黑曲霉和青霉的体外抑菌活性。方法管碟法。结果赤芝菌体三萜化合物对大肠杆菌、金黄色葡萄球菌的生长均具有显著抑制作用;对枯草芽孢杆菌、青霉和黑曲霉抑制作用较弱;该三萜样品对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、黑曲霉和青霉的最小抑菌浓度分别为25,25,50,100和50 mg/ml。结论赤芝发酵菌体中三萜对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和青霉有明显的抑制作用。     

Myrislignan Attenuates Lipopolysaccharide‐induced Inflammation Reaction in Murine Macrophage Cells Through Inhibition of NF‐κB Signalling Pathway Activation

Myrislignan is a new kind of lignan isolated from Myristica fragrans Houtt. Its antiinflammatory effects have not yet been reported. In the present study, the antiinflammatory effects and the underlying mechanisms of myrislignan in lipopolysaccharide (LPS)‐induced inflammation in murine RAW 264.7 macrophage cells were investigated. Myrislignan significantly inhibited LPS‐induced production of nitric oxide (NO) in a dose‐dependent manner. It inhibited mRNA expression and release of interleukin‐6 (IL‐6) and tumour necrosis factor‐α (TNF‐α). This compound significantly inhibited mRNA and protein expressions of inducible NO synthase (iNOS) and cyclooxygenase‐2 (COX‐2) dose‐dependently in LPS‐stimulated macrophage cells. Further study showed that myrislignan decreased the cytoplasmic loss of inhibitor κB‐α (IκB‐α) protein and the translocation of NF‐κB from cytoplasm to the nucleus. Our results suggest that myrislignan may exert its antiinflammatory effects in LPS‐stimulated macrophages cells by inhibiting the NF‐κB signalling pathway activation. Copyright © 2012 John Wiley & Sons, Ltd.     

三七皂甙Rg_1及Rb_1对离体大鼠肝细胞损害的保护作用

目的:探讨三七皂甙 Rg_1及 Rb_1对脂多糖(lipopolysaccharide,LPS)引起急性肝损害的保护作用。方法:用胶原酶原位灌流法分离肝细胞,用透射电镜观察肝细胞形态学变化,用 Fura-2-AM 测定细胞内游离钙离子浓度。用[~3H]油酸标记的生物膜法测定分泌型磷脂酶 A_2(secretory phospholipase A_2,sPLA_2),用ELISA 法检测前列腺素 E_2(PGE_2)的活性,用 RT-PCR 检测 sPLA_2基因表达。结果:三七皂甙 Rg_1、Rb_1能显著抑制 LPS 所致的肝细胞凋亡与坏死,明显抑制 LPS 引起的胞内 Ca~(2+)超载,抑制 sPLA_2的活性、PGE_2水平和 sPLA_2基因的表达。结论:三七皂甙 Rg_1、Rb_1能显著抑制急性肝损害可能与其对钙的拮抗和对 sPLA_2的抑制作用有关。