庆大霉素和聚维酮碘降低内眼手术中前房水污染的前瞻性研究

目的观察眼前节内眼手术中由前房穿刺导致的前房水污染情况，并比较术前用庆大霉素注射液、5g·L^-1国产聚维酮碘和50g·L^-1进口聚维酮碘冲洗结膜囊降低前房污染的效果。方法300例（300眼）行眼前节手术的患者。随机分为3组。患者术前1d用3g·L^-1氧氟沙星滴眼液滴患眼6次。术前约5min依照分组分别用8g·L^-1庆大霉素注射液、5g·L^-1国产聚维酮碘及50g·L^-1进口聚维酮碘冲洗结膜囊。术中用前房穿刺刀做好透明角膜辅助切口，没有用灌注液之前，采集前房水作细菌培养。结果用药后庆大霉素组、5g·L^-1聚维酮碘组及50g·L^-1聚维酮碘组前房水细菌培养阳性率分别为2．7％、6．5％和5．3％，平均为4．9％，3组间差别没有统计学意义（P〉0．05）。结论前房穿刺可以将附着在眼表的细菌带入前房。术前用庆大霉素注射液、5g·L^-1国产聚维酮碘和50g·L^-1进口聚维酮碘冲洗结膜囊清除眼表细菌的效果相似。     

眼前节内眼手术应用5％吡咯烷酮碘两种不同用法的前瞻性随机化比较研究

目的：确定5％吡咯烷酮碘采用两种不同的方法（结膜囊滴两滴和10ml溶液穹窿结膜冲洗）对降低结膜囊菌群的功效。方法：在这项前瞻性对照实验中。将行眼前节手术的200只眼随机分为对照组和研究组。两组病例术前均给予氧氟沙星滴眼液局部点眼和5％吡咯烷酮碘眶周区域皮肤擦洗消毒。术前对照组眼结膜囊内给予2滴5％吡咯烷酮碘溶液，研究组眼用5％吡咯烷酮碘溶液冲洗穹窿结膜。分别于术前和术后4个时间点行结膜细菌培养。结果：研究组78只眼中20只眼（26％）术前结膜细菌培养阳性：对照组94只眼中40只眼（43％）为阳性（P=0．02）。手术结束时。研究组78只眼中14只眼（18％）、对照组94只眼中30只眼（32％）结膜细菌培养阳性（PP=0．05）。结论：手术时应用5％吡咯烷酮碘溶液冲洗穹窿结膜与结膜囊仅点入两滴相比。前者显著降低了结膜细菌培养阳性率。     

庆大霉素和聚维酮碘对结膜囊细菌清除率的比较研究

目的 探讨庆大霉素、0.5%聚维酮碘及5.0%聚维酮碘清除结膜囊细菌的效果及其副作用.方法 采用比较性研究,选择2006年3月至5月在武汉爱尔眼科医院行眼前节手术的300例患者(300只眼),采用随机数字法分为3组.手术前1 d,用0.3%氧氟沙星眼液滴患眼6次.表面麻醉后术前约5 min依照不同分组分别用0.8%庆大霉素注射液、0.5%聚维酮碘及5.0%聚维酮碘冲洗结膜囊.在下列时间采集患者结膜囊标本做细菌培养和药物敏感性试验:入院时未用任何药物之前的基准培养;使用0.3%氧氟沙星滴眼液1 d后;未用药物冲洗结膜囊前;手术刚开始时;冲洗结膜囊约5 min后;手术结束时.在手术开始时,采集前房水标本做细菌培养.各组间培养阳性率的比较,采用Pearson卡方检验.结果 未用药前,0.8%庆大霉素注射液组、0.5%聚维酮碘组及5.0%聚维酮碘组结膜囊培养阳性率分别为79.7%、76.6%及77.8%,差异无统计学意义(X2=0.28,P=0.86);手术完成后,3组结膜囊培养阳性率分别为11.7%、8.3%及6.3%,差异也无统计学意义(X2=1.74,P=0.41).前房水培养平均阳性率为4.9%,3组之间比较差异无统计学意义(X2=1.21,P=0.54).患者未出现对聚维酮碘有明显的不良反应.结论 表面麻醉后术眼结膜囊内使用0.5%国产聚维酮碘和5.0%进口聚维酮碘均较安全;术前用0.8%庆大霉素注射液、0.5%聚维酮碘及5.0%聚维酮碘冲洗结膜囊清除眼表细菌的效果相似.     

白内障超声乳化术中灌注液加入头孢呋辛对房水细菌污染的控制作用

目的　评估白内障超声乳化术中灌注液加入头孢呋辛对房水细菌污染的控制作用。方法　选取2017年5月至8月在青岛眼科医院接受白内障超声乳化手术治疗的患者为研究对象，根据术中灌注液的不同分为两组，对照组99例（102眼）使用平衡盐溶液灌注液；试验组93例（100眼）使用含头孢呋辛（1.5 g·L^-1）的平衡盐溶液灌注液。两组患者分别于入院时（未局部应用抗菌药物前）、术前（聚维酮碘冲洗结膜囊后）行结膜囊细菌培养，于手术结束时行房水细菌培养，并对两组患者结膜囊及房水细菌培养结果进行比较。结果　入院时，对照组与试验组结膜囊细菌培养阳性率为50.00%和51.00%，差异无统计学意义（χ²=0.020，P=0.887）；术前，对照组与试验组结膜囊细菌培养阳性率为25.5%和27.0%，差异无统计学意义（χ²=0.059，P=0.807）；手术结束时，对照组与试验组房水细菌培养阳性率为5.9%和0，差异有统计学意义（F=6.062，P=0.029）。结论　使用含头孢呋辛（1.5 g·L^-1）的灌注液能减少白内障超声乳化术的房水细菌污染。     

老年人内眼手术前结膜囊细菌培养的分析

目的 了解老年人内眼手术前的结膜囊细菌情况。方法 对老年患者279例(350眼)术前进行结膜囊细菌培养，并对阳性者作药敏试验。结果 术前结膜囊细菌培养阳性率为17．43％。药敏试验结果：敏感药物为氧氟沙星、先锋霉素、庆大霉素等。结论 老年人内眼手术前用敏感抗生素滴眼，并常规行结膜囊细菌培养及药敏试验是必要的。     

白内障手术前后结膜囊细菌学分析

目的：探讨白内障术前、术后结膜囊细菌学分布状态，为临床用药提供依据。方法：将无睑缘炎、结膜囊外观清洁、泪道冲洗通畅的白内障住院患者94例分别于入院未用抗生素眼液点眼前、手术消毒前及术后第1天拆开眼垫换药前进行结膜囊细菌培养。结果：未用抗生素眼液点眼前的结膜囊(正常结膜囊)细菌培养阳性率为56．4％，经0．3％氟哌酸眼液点眼后结膜囊细菌培养阳性率为0，但术后第1天拆开眼垫后结膜囊的细菌培养阳性率为5．3％。结论：正常结膜囊可以有各种致病菌或非致病菌存在，即使术前结膜囊细菌培养为无菌生长，但术后第1天仍然可能有结膜囊细菌存在，提示白内障术前及术后均应加强抗生素眼液点眼，以减少眼内炎的发生。     

预防白内障手术后感染的术中应对策略探讨

目的 探讨老年性白内障临床路径预防术后感染性眼内炎的术中措施及效果.方法 选择120例120眼白内障手术患者,随机分为对照组(A组)60例60眼术前、术毕应用复方氯化钠注射液冲洗结膜囊.研究组(B组)60例60眼术前、术毕应用0.1％聚维酮碘溶液(0.1％PVP-I,深圳市安多福消毒高科技股份有限公司生产,批号:粤卫消证字{2016}第9087号)结膜囊进行消毒,同时术中灌洗液中加入妥布霉素.分别取术前、术前冲洗结膜囊后、术毕冲洗结膜囊前后及术后第1天的结膜囊标本做细菌培养及药敏.取术毕前房房水的细菌培养及药敏.结果 1结膜囊细菌培养:a、术前A组阳性16例,B组阳性17例,两组的差异无明显统计学意义(x2 =0.04,P＞0.05).b、术前冲洗结膜囊后,A组阳性14例,B组阳性2例,两组的差异有明显统计学意义(x2=10.29,P＜0.05).c、术毕A组阳性17例,B组阳性5例,两组的差异明显统计学意义(x2=8.02,P＜0.05).d、术毕冲洗结膜囊后,A组阳性15例,B组阳性1例,两组的差异具有明显统计学意义(x2=14.14,P＜0.05).e、术后第1天A组阳性19例,B组阳性7例,两组的差异具有明显统计学意义(x2=8.51,P＜0.05).2.A组术毕前房水细菌培养阳性率3.33％,而B组房水细菌培养为阴性明显低于A组.所有患者观察术后3个月无眼内炎等并发症.结论 白内障术中应用0.1％PVP-I溶液结膜囊进行两次消毒,灌洗液中加入妥布霉素,能够进一步减少结膜囊细菌,保持房水无菌化,在预防感染上实用安全,效果更可靠.     

白内障患者术前术中细菌培养及药物干预研究

目的：研究年龄相关性白内障患者睑缘(睫毛根部睑板腺开口处)和结膜囊应用不同抗生素滴眼液滴眼及不同结膜囊冲洗液冲洗结膜囊后的灭菌效果。

方法：选取2015-01/11在我院拟行白内障超声乳化手术的年龄相关性白内障患者216例216眼随机分成A(72眼)、B(72眼)、C(72眼)三组,术前分别采用3g/L妥布霉素滴眼液、5g/L左氧氟沙星滴眼液及上述两种滴眼液点眼; 再随机分为莫西沙星组(110眼)和聚维酮碘组(106眼),分别于术野常规消毒后手术正式开始前使用1.6g/L莫西沙星和5g/L聚维酮碘冲洗结膜囊。分别于入院未用药前、手术未消毒前及结膜囊冲洗后采集术眼睑缘和结膜囊标本,术毕采集结膜囊标本并抽取前房水进行常规细菌培养及药敏试验。

结果：白内障患者术前睑缘、结膜囊细菌培养阳性率分别为86.6%、25.0%。A、B、C组患者应用不同抗生素滴眼液点眼前后睑缘细菌培养阳性率分别为86.1%和36.1%、84.7%和31.9%、88.9%和30.6%; 结膜囊细菌培养阳性率分别为25.0%和11.1%、26.4%和11.1%、23.6%和8.3%。采用莫西沙星和聚维酮碘组进行结膜囊冲洗前后睑缘细菌培养的阳性率分别为33.6%和10.9%、32.1%和10.4%; 结膜囊细菌培养的阳性率分别为10.0%和0、10.4%和0。

结论：白内障术前联合使用3g/L妥布霉素和5g/L左氧氟沙星滴眼液点眼清洁睑缘和结膜囊效果更佳,5g/L聚维酮碘和1.6g/L莫西沙星均可作为白内障术前结膜囊冲洗液使用。     

白内障术前两种不同浓度碘伏液冲洗结膜囊的临床观察

目的比较两种不同浓度（0．05％与0．025％）碘伏液（聚维酮碘）在白内障术前冲洗结膜囊后,患者角膜荧光染色、主觉症状、结膜囊细菌培养等方面的差异,以期找到更安全、有效的白内障术前冲洗结膜囊的碘伏液浓度。方法选择住院行白内障超声乳化术的患者83例（83只眼）,随机分为A、B两组,术前分别采用0．05％碘伏液（A组）、0．025％碘伏液（B组）冲洗结膜囊,比较两组术后患者主观症状、术眼眼表荧光素钠染色评分的差异,以及术前、术后结膜囊采样细菌学培养情况。结果A、B两组浓度的聚维酮碘稀释液均能达到相同的结膜囊细菌消毒效果,但B组较之A组术后患者眼部感觉更舒适,同时客观的眼表损伤更轻微。结论较低浓度（0．025％）的聚维酮碘液在保证有效清除结膜囊细菌的同时,还能明显地降低术后患者的主观不适症状和客观的眼表损伤体征,有利于视功能的更快恢复,可作为临床推广应用。     

内眼手术眼部消毒前、后睑缘、结膜囊细菌培养分析

目的：通过对内眼手术眼局部消毒处理前后。睑缘、结膜囊细菌培养的分析。寻找出一种无菌、抑菌、无毒、无刺激的冲洗液。方法：白内障、青光眼患者60例（60眼）手术当日做术眼清洗处理前及清洗消毒处理后睑缘、结膜囊的普通细菌培养。结果：清洗处理前。60眼进行睑缘细菌培养,24眼有菌生长（阳性率40%）;清洗消毒处理后,7眼仍有菌生长（29.1%),清洗处理前,结膜囊细菌培养,17眼有菌生长（阳性率28.3%);清洗消毒后,5眼仍有菌生长（29.4%)。讨论：配制具有清洁、抑菌作用的冲洗液冲洗结膜囊,及具有去污、去脂、无菌、无菌、无刺激的皮肤清洁剂,可以进一步提高手术野及无菌率,保证手术的安全性和可靠性。     

手术切除联合自体血清滴眼液治疗翼状胬肉的疗效观察

目的观察翼状胬肉切除术后局部滴用自体血清滴眼液对创面修复的临床疗效。方法治疗组翼状胬肉16眼采用单纯翼状胬肉切除术联合局部滴用自体血清滴眼液治疗,对照组翼状胬肉16眼采用单纯翼状胬肉切除术治疗。结果治疗组术后3d时自觉症状明显减轻,2周时11眼充血完全减退;对照组4d时畏光、流泪开始减轻,2周时6眼充血完全减退。角膜上皮平均修复时间治疗组（3．34±1．52）d与对照组（4．45±1．49）d相比差异有统计学意义（t=2．09,P〈0．05）。治疗组术后11眼视力有提高,对照组4眼视力提高,两组对比差异有统计学意义（χ^2=5．24,P〈0．05）。治疗组1眼复发,对照组2眼复发,两组均无感染。结论翼状胬肉切除术后局部应用自体血清滴眼液可缩短角膜上皮的修复时间,减少了复发和感染机会。自体血清滴眼液制作简单,患者耐受性好。     

Enalaprilat and enalapril maleate eyedrops lower intraocular pressure in rabbits

Purpose: This study aimed to develop low‐viscosity aqueous eyedrops containing enalaprilat and its prodrug enalapril maleate in solution, and to evaluate the eyedrops in rabbits. Methods: Aqueous eyedrops with hydroxypropyl‐β‐cyclodextrin containing 0.01–2.9% (w/v) enalaprilat, 1.0% (w/v) enalapril maleate with cyclodextrin or 0.5% (w/v) timolol were prepared. The eyedrops were administered to rabbits and intraocular pressure (IOP) was measured at various time intervals after the administration and the results (mean of 10 experiments ± standard error of the mean) are expressed as the change from baseline (24.7 ± 3.3 mmHg). Results: Enalaprilat possessed sufficient stability to be formulated as an aqueous eyedrop solution with a shelf‐life of several years at room temperature. The maximum decline in IOP after topical administration of one drop of 2.9% enalaprilat solution was 6.2 ± 0.7 mmHg at 4 hours after administration. Duration of activity exceeded 10 hours. A 1% enalaprilat solution lowered IOP by 4.4 ± 0.8 mmHg at 4 hours after administration and had similar duration, and was more potent than 0.5% timolol. The enalapril maleate eyedrops resulted in delayed action, showing maximum potency at 10–22 hours after administration and duration of up to 32 hours. Conclusions: Enalaprilat eyedrops lower IOP in rabbits. The decline in IOP is proportional to the concentration of dissolved enalaprilat in low‐viscosity aqueous eyedrop formulations.     

FK-506抑制高危角膜移植免疫排斥反应的研究

目的 :探讨FK -5 0 6抑制高危角膜移植免疫排斥反应临床应用的可行性及有效性。方法 :应用前瞻性评估研究方法 ,将行高危角膜移植术 (全角膜移植术、带巩膜环的全角膜移植术、血管化角膜的角膜移植术及角膜再移植术 ) 5 6例 (5 6只眼 )患者按随机原则进行分组 (投药组及对照组 ) ,投药组 (2 8只眼 )滴用 0 5mg/ml的FK -5 0 6滴眼液联合典必珠滴眼液 ,对照组 (2 8只眼 )滴用 1%CsA滴眼液联合典必殊滴眼液 ;平均随访 8 1个月 ,以术后视功能、植片透明维持时间、植片新生血管、水肿及混浊程度作为临床主要评估指标。结果 :随防期内投药组及对照组角膜移植片免疫排斥反应发生率分别为 63 6%及 95 2 % ,差异有显著性 (χ2 =4 72 ,P <0 0 5 )。用药期间未发现该药有任何毒副作用。结论 :局部应用FK -5 0 6可有效抑制高危角膜移植免疫排斥反应的发生。     

鱼腥草滴眼液治疗流行性角结膜炎的临床疗效观察

目的观察中药制剂鱼腥草滴眼液对流行性角结膜炎的临床疗效和安全性。方法本文为随机、对照临床试验。将40例47眼流行性角结膜炎患者随机分为鱼腥草治疗组和病毒唑对照组,每组各20例。试验组滴用鱼腥草滴眼液6次·d-1,每次1滴。对照组滴用0.5%病毒唑滴眼液6次·天-1,每次1滴,疗程为10d。在用药后第1d、4d、7d、10d复查。对2组患者的症状、体征进行观察评分,同时对不良反应进行观察。结果试验组和对照组治疗有效率分别为80.0%和81.8%,2组有效率无统计学差异(P=0.242)。中药鱼腥草滴眼液能明显改善流行性角结膜炎患者的主要症状和体征,这种改变的趋势与0.5%病毒唑滴眼液相似。试验组1例发生不良反应,对照组无不良反应发生。结论鱼腥草滴眼液用于治疗流行性角结膜炎效果与0.5%病毒唑滴眼液相同,安全性好。     

Bandage contact lens and topical indomethacin for treating persistent corneal epithelial defects after vitreoretinal surgery

PURPOSE: Persistent corneal epithelial defect is a complication of vitreoretinal surgery. We assessed the efficacy of using bandage contact lenses in conjunction with topical indomethacin to facilitate wound healing and control pain safely. METHODS: Patients presenting with a persistent corneal epithelial defect after vitreoretinal surgery were assigned randomly to 1 of 2 treatment groups. Patients in both groups received a therapeutic contact lens and chloramphenicol eyedrops, whereas group A received indomethacin eyedrops and group B received placebo eyedrops. This study was conducted in a single-masked randomized fashion. Patients were evaluated daily to monitor subjective symptoms and to measure the maximum and minimum dimensions of the epithelial defects. RESULTS: Sixteen patients were analyzed. The subjective evaluation of photophobia, foreign-body sensation, and tearing on the day after starting treatment did not differ significantly between the 2 groups (P > 0.05), whereas the difference in the mean pain decrease between groups A and B at day 1 was significant (P = 0.01). The rate of epithelial healing quantified as the days needed to heal the epithelial defect did not differ between the 2 groups (P = 0.823). CONCLUSIONS: Our study showed that topical indomethacin ophthalmic solution improves comfort in patients with a persistent corneal epithelial defect (after vitreoretinal surgery) treated with bandage contact lenses.     

Comparing the effects of travoprost and brinzolamide on intraocular pressure after phacoemulsification

PURPOSE: To evaluate the intraocular pressure (IOP) lowering effect of travoprost and brinzolamide within the first 24 h after phacoemulsification cataract surgery. METHODS: This prospective, randomized, double-masked, controlled study comprised 90 eyes of 90 consecutive patients with senile cataract who had uneventful phacoemulsification surgery. Eyes in the first group received travoprost 0.0015%, second group received brinzolamide 1%. Eyes in the third group received balanced salt solution and were used as control. One drop was instilled immediately after surgery. IOP was measured 24 h preoperatively, 6 and 24 h postoperatively. Analysis of variance, Student's-t and chi2-tests were used for statistical analyses. RESULTS: Preoperatively IOP was not significantly different among the three groups (P = 0.653). At 6 and 24 h postoperatively IOP was lower in both travoprost and brinzolamide group when compared to control group (P = 0.018 and 0.015 at 6 h, P = 0.010 and 0.007 at 24 h between travoprost and brinzolamide group was not significant (P = 0.744 at 6 h and P = 0.672 at 24 h). CONCLUSION: Both travoprost and brinzolamide significantly lowered IOP after small incision phacoemulsification cataract surgery within the first 24 h without any side effect.     

FK-506抑制同种异体角膜缘移植术后免疫排斥反应的研究

目的：探讨ＦＫ－５０６抑制角膜缘干细胞移植术后免疫排斥反应的临床可行性与有效性。方法：应用前瞻性评估研究方法,将角膜缘移植术后病例６４只眼按随机原则分投药组及对照组各半,投药组应用０．５％ＦＫ－５０６滴眼液,对照组应用１％ＣｓＡ滴眼液。平均随访期半年,以术后角膜缘植片新生血管、水肿、混浊及溶解程度为临床主要评估指标。结果：随访期内在新生血管指数、上皮排斥发生率、移植排斥指数及假性胬肉指数四项指标投     

The influence of diclofenac ophthalmic solution on the intraocular pressure-lowering effect of topical 0.5% timolol and 0.005% latanoprost in primary open-angle glaucoma patients

The aim of this randomized, prospective, masked clinical study has been to verify the influence of a non-steroidal anti-inflammatory drug ophthalmic solution on intraocular pressure reduction induced by 0.5% timolol and 0.005% latanoprost eyedrops in patients affected by primary open-angle glaucoma. Thirty-two glaucomatous patients, compensated with 0.5% timolol, were randomized into two study groups (A and B). Timolol was continued for the first 2 weeks in all subjects. On the 15th day, in both groups timolol was replaced by latanoprost, and this regimen lasted up to the end of the follow-up (8 weeks). At the beginning of the 2nd week of the study, group A additionally started a 5-week therapy with topical 0.1% diclofenac; during the same period, group B received placebo eyedrops with identical modalities. Intraocular pressure was recorded at 7-day intervals during the first 7 weeks and at the 10th week. Non-steroidal anti-inflammatory drug and placebo did not modify the effect of timolol on intraocular pressure. In both groups, latanoprost induced a significant decrease in intraocular pressure. Diclofenac-treated patients exhibited a marked fall in intraocular pressure (p<0.01), whereas in placebo-treated patients, this diminution was less noticeable (p<0.05). After diclofenac withdrawal, in group A intraocular pressure significantly increased (p<0.01), remaining approximately at the same level up to the end of the study. In group B, at the same checks no significant variations in intraocular pressure occurred. In primary open-angle glaucoma patients, diclofenac significantly enhances the hypotensive effect of latanoprost, without influence on timolol efficacy. Because non-steroidal anti-inflammatory drugs are widely employed in medical practice, supplementary ophthalmologic checks should be scheduled during the co-administration of these compounds and prostaglandin analogues.     

复美新抑制玻璃体视网膜手术中瞳孔缩小的临床研究

目的　研究复美新滴眼液在玻璃体视网膜手术中抑制瞳孔缩小的作用。方法　对照组术前使用阿托品和复方托吡卡胺滴眼液散瞳 ,实验组加用复美新滴眼液 ,比较两组的区别。结果　实验组平均瞳孔缩小 0 2 5mm ,明显小于对照组缩小值 1 3 1mm (P <0 0 1)。根据手术方法不同将患者分为 3组比较 ,手术结束时平均瞳孔缩小值实验组均小于对照组 (P <0 0 1)。实验组中增生型糖尿病视网膜病变患者手术结束时平均瞳孔缩小值为 0 5mm ,明显小于对照组中糖尿病患者平均缩小值 1 5mm (P <0 0 1)。根据手术时间不同比较 ,实验组瞳孔缩小值均小于对照组 (P <0 0 1)。结论　复美新可有效抑制玻璃体视网膜手术中瞳孔缩小 ,尤其对于时间较长的复杂手术效果更显著。     

Effect of beta-blocker eyedrops on corneal epithelial barrier function

To investigate the long-term effect of a topically applied beta-blocker on human corneal epithelium, the corneal epithelial barrier function and the superficial cell area of the corneal epithelium were evaluated. Seventeen normal healthy volunteers (without medication), 7 cataract patients (treated with pyrenoxine eyedrops) and 7 glaucoma or ocular hypertension patients (treated with 0.5% timolol maleate) were assigned to this study. The eyedrops had been used on a daily basis for at least 3 months. In the evaluation of corneal epithelial barrier function, fluorescein uptake was measured using a slitlamp fluorophotometer after application of 3 microl of 0.5% fluorescein for 10 min. In the evaluation of the superficial cell area, the central corneal epithelium was measured by tandem scanning confocal microscopy (TSCM). The healthy control and timolol groups were compared. Corneal fluorescein uptake in the healthy control, pyrenoxine and timolol groups was 20.3 +/- 3.2, 21.5 +/- 4.0 and 76.2 +/- 30.0 ng/ml (mean +/- standard error), respectively. There was a significantly higher fluorescein uptake in the timolol group compared to the pyrenoxine group (p = 0.0088) and the healthy control group (p = 0.0055). TSCM showed no significant difference in the superficial cell areas of the corneal epithelium between the healthy control and timolol groups. beta-Blocker eyedrops decreased the corneal epithelial barrier function. Their application was not accompanied by any biomicroscopic change in the superficial cell area.