HISTOPATHOLOGIE DE LA MOELLE DANS LE SYNDROME MYELODYSPLASTIQUE

62 cases of myelodysplastic syndrome have been examined histopathologically There were 30 males and 32females with ages from 20 months to 74 years old. 31 cases (50%) showed erythroblastic islands, 51 cases (82.2%) abnormal localization of immature precursors (ALIP) and 39 cases (62.9%) micromegakaryocytes. ALIP was present in 41/45 of cases with lencocytosis. Only 16.1% of the cases presented the coexistences of the 3 typical histopathological changes while 100% of the cases showed 2 of the three changes. The correlation of the clinical and the histopathological diagnosis was 76%. Differential diagnosis were discussed.     

(E)VALUATION DE L'EXPRESSION DES R(E)GULATEURS DU CYCLE CELLULAIRE EN G1/S DANS LE CARCINOM(E)PIDERMO(I)DE DE L'SOPHAGE

Objective To study the expression of p16, cyclin D1, Rb and phosphorylated Rb(Ser795) in the esophageal squamous cell carcinoma. Methods Immunohistochemistry was performed and analysed on 34 cases of paraffin-embedded tissues. Results The expression of phosphorylated Rb(Ser795) was positively correlated to that of cyclin D1 (r=0.401, P=0.021) and inversely to that of p16 (r=-0.348, P=0.044). In stepwise regression and the best subset regression, the expression of p16 (P=0.034) and phosphorylated Rb(Ser795) (P=0.030) were the only determinants of the mitotic index. Conclusion The expression of phosphorylated Rb(Ser795) could be considered as a mark of the interaction between p16 and cyclin D1. The detection of phosphorylated Rb, p16 and cyclin D1 will be possibly helpful to the oncogenesis investigation on the esophageal carcinoma.     

DETECTION DE LA MUTATION DU GENE HMLH1 DANS LE CANCER COLO-RECTAL PARELECTROPHORESE CAPILAILRE ASSOCIEE A LA FLUORESCENCE LASER-INDUITE

Objectif L'objectif de ce travail est d'etablir une methode d'analyse du polymorphisms de la conformation monochaine (PCMC) , capable de detecter la mutation ponctuelle du gene hMLHI dans le cancer colique en utilisant l'l ctrophor se capillaire associee a la fluorescence laser-induite (FLJ-EC). Methodes Les exons 12 du gene hMLHl du sang peripherique de 42 patients de cancer colo-rectal sporadique et 20 sujets sains temoins sont amplifies par la PCR. Le PCMC des produits du PCR est analyse par FLJ-EC. Les echantillons anor-maux ont ete confirmes par sequen age EC. Les effets de la concentration du milieu (LPA) , de la temperature de separationcet du voltage de separation sur le comportement de EC ont ete aussi studies. Resultats L'analyse de PCMC par la methode FLJ-EC a retrouve une mutation heterozygote chez 4 des 42 patients. La mutation ponctuelle de T1151A a ete objectivee par la sequen age EC de ces echantillons, aucune mutation n'a ete retrouvee chez les 20 sujets sains temoins. La separation des pics d'ADN monochainaux a ete facilitee par une legere augmentation de la concentration de LPA (4%-6% ) , une legere baisse de la temperature de separation (20℃) et une legere elevation du voltage de separation (9kV). Conclusion Une concentration adequate de LPA, une temperature de separation appropriee et un voltage de separation bien approprie ameliorent considerablement l'efficacite du FLJ-EC. L'application de cette methode pour detecter la mutation ponctuelle du gene hMLHl est d'une rapidite, d'une efficacite, et d'uneereproductivit consid rabies. Cette methode de realisation facile reste tres prometteur pour le depistage rapide et massive des mutations genetiques.     

泰必利治疗抽动-秽语综合征10例疗效分析

泰必利是新合成的精神安定药,作用于中脑边缘系统并有抗多巴能活性,治疗精神运动行为障碍。本文用秦必利50～100mg 每日3次治疗抽动-秽语综合征10例,其中显效6例,有效3例。其优点不产生锥体外系症状,无明显毒副反应。     

氟哌啶醇治疗儿童抽动-秽语综合征时不应忽视的副作用

应用氟哌啶醇治疗儿童抽动-秽语综合征时可以产生某些特殊的易被忽视的副作用。21名患儿中,4人出现与药物剂量有关的心境恶劣,1人发生静坐不能,1人产生敌意和攻击行为,1人偶而意识模糊,2人出现迟发性运动障碍。上述副作用在减低药量或停药后均消失。临床医生应对这类副作用提高警觉。     

L''''ANGINE DE POITRINE PRECEDENT L''''INFARCTUS DU MYOCARDE:RELATION AVEC LES LESIONS CORONAIRES ET LA CIRCULATION COLLATERALE

To determine the relationship between angina pectoris before myocardial infarction (MI) and the extent of coronary artery disease and collateral circulation, 28 patients with (group Ⅰ)and 22 patients without (group Ⅱ) angina one month prior to acute MI underwent left ventriculography and coronary arteriography within 6 months of a first transmural MI. 23 patients in group Ⅰbut only 6 patients in group Ⅱhad multivessel disease. Despite a similar incidence of total occlusion of infarct-related artery, the collateral score in group Ⅰwas significantly higher than that in group Ⅱ. Abnormal regional left ventricular motion score was lower in group Ⅰthan in group Ⅱ. The study suggests that a history of angina before MI may predict the presence o f multivessel disease and the formation of collateral circulation in patients with MI.     

VERAPAMIL TREATMENT OF TORSADES DE POINTESVENTRICULAR TACHYARRHYTHMIA IN PARTICULAR PATTERN

This paper reports two cases of "Torsades depointes" ventricular tachyarrhythmia (TPVT) in particular form. The electrocardiographic characteristicsshowed the presence of abnormally short couplingintervals of premature ventricular contractions(PVCs)as well as the PVCs initiating tachycardias in the set-ting of normal QT intervals. The tachycardias weresusceptible to verapamil and refractory to other an-tiarrhythmic agents. Either triggered activity or re-entry due to slow inward current is considered forthe mechanism of this type of tachyarrhythmia.     

EN BLOC TRANSPLATION OF KIDNEY AND WHOLE PANCREAS WITH A SEGMENT OF DUODENUM IN RATS

For meeting the clinic needs in simultaneous pancreas and kidney transplantation (SPK). we successfully establish a syngeneie SPK transplatation model in Lewis rats. The results indicate that this model is feasible with a 82. 6% successful rate of operation and a 69.6% survival rate in the first postoperative week. In long-term survived rats, the blood supplies are well established, function of the grafts (pancreas and kidney) maintains normal. This model is suitable for theoretical reserach in SPK transplantation for its reasonable physiology with pancreatic juice drained into intestine and reduced postoperative complications in urinary tract and carbohydrate metabolism.     

人白细胞蛋白质的提取及双向电泳分析

目的 :建立人白细胞蛋白质提取及双向电泳分析方法 ,为构建白细胞在不同生理或病理条件下的蛋白质表达谱或进行蛋白质表达的差异分析奠定基础。方法 :取人全血 ,分离白细胞 ,提取蛋白质 ,双向电泳分离 ,考马斯亮蓝R - 2 5 0染色获得蛋白质的电泳分离图谱 ,利用PDQuest2D分析软件进行图像分析 ,结合SWISSSPORT蛋白质数据库对白细胞蛋白质斑点进行初步鉴定。结果 :所得双向电泳图谱中各点分离清晰 ,无明显横向或纵向拖尾 ,分析显示图谱上有 2 12个蛋白质斑点 ,主要集中在pH4 .75～ 6 .81之间 ,其中高丰度蛋白斑点有 36个点 ,低丰度的蛋白质斑点约 176个 ,对其中 6个高丰度蛋白质斑点初步鉴定可知这些蛋白质为 :CAN2 (MW :77975 .95pI:4 .84 )、I17R(MW :94 4 15 .6 3pI:4 .90 )、CARF(MW :5 130 2 .6 5pI:5 .82 )、FIG1(MW :6 8184 .0 6pI:6 .2 9)、PIGR(MW :82 96 9.32pI:5 .2 2 )、F16P(MW :36 781.2 8pI:6 .18)。结论 :本实验建立了人白细胞蛋白质的双向电泳分析方法 ,图谱分离、染色效果好 ,能满足 2 -DE专业软件分析的要求 ,为后续白细胞的蛋白质组研究奠定了基础。     

高脂血症沙鼠组织中某些酯酶活性的变化及药物降脂作用的研究

通过给沙鼠食入高脂饲料建立沙鼠高脂血症模型，同时观察肝脏和心肌中各种酯酶的活性变化，进而对中药鸦胆子油乳剂的降脂作用及降脂机制进行了探讨。结果表明，在高脂组，肝脏组织总脂解酶活性（ＬＡ）降低；心肌和肝脏的脂蛋白脂肪酶（ＬＰＬ）活性均明显降低，而肝甘油三酯脂肪酶（ＨＴＧＬ）活性无明显改变。给高脂血症沙鼠食入鸦胆子油乳剂（ＢＪＯ）后，肝脏及心肌中的ＬＡ活性和ＨＴＧＬ活性无明显改变，但ＬＰＬ活性增高     

胰十二指肠切除术后肠内和肠外营养支持的对比研究

目的对比观察胰十二指肠切除术(PD)后肠内营养(EN)和肠外营养(PN)对病人血清蛋白、体重、氮平衡、上臂周径和手术后并发症、住院时间的影响,比较二者的费用。方法46例PD患者随机分为两组:EN组(22例)术中空肠造口,术后24 h开始EN;PN组(24例)术后1 d起给予PN。两组采用等热量、等氮量方案,比较两组患者的术后营养状况变化、并发症发生率以及营养支持费用。结果PD后EN和PN均能改善患者的营养状态。EN组术后平均住院日较PN组短[(21.4±7.6)d vs (24.1±9.3)d,P>0.05]。EN组较PN组并发症发生率低,但差异不显著(P>0.05)。EN组营养支持费用则明显少于PN组[(1290.0±76.8)元vs (4072.0±146.4)元,P<0.01]。结论PD后经空肠造口管给予EN与PN一样能够达到营养支持的目的,并具有安全、费用低廉等优点。