沉默环状RNA hsa_circ_0000591表达对肝癌细胞增殖及迁移的影响

目的 探讨环状RNA hsa_circ_0000591在肝细胞癌(hepatocellular carcinoma,HCC)组织以及肝癌细胞系中的表达,及其对肝癌细胞增殖和迁移的影响.方法 收集2014—2018年广西医科大学附属肿瘤医院手术切除的84例HCC组织及其相应癌旁正常组织标本,采用qRT-PCR检测hsa_...     

Characterization of the small RNA transcriptomes of cell protrusions and cell bodies of highly metastatic hepatocellular carcinoma cells via RNA sequencing

Increasing evidence suggest that hepatocellular carcinoma (HCC) HCCLM3 cells initially develop pseudopodia when they metastasize, and microRNAs (miRNAs/miRs) and circular RNAs (circRNAs) have been demonstrated to serve important roles in the development, progression and metastasis of cancer. The present study aimed to isolate the cell bodies (CBs) and cell protrusions (CPs) from HCCLM3 cells, and screen the miRNAs and circRNAs associated with HCC infiltration and metastasis in CBs and CPs. The Boyden chamber assay has been confirmed to effectively isolate the CBs and CPs from HCCLM3 cells via observation of microtubule immunofluorescence, DAPI staining and nuclear protein H3 western blotting. Following high-throughput sequencing of the successfully isolated CBs and CPs, 64 pairs of miRNAs, including 23 pairs of upregulated genes and 41 pairs of downregulated genes, and 260 sets of circRNAs, including 127 upregulated genes and 133 downregulated genes, were significantly differentially expressed, using the following criteria: HP/HB ratio, fold change ≥|1.5|, P<0.05). PCR analysis verified that changes in the expression levels of hsa-let-7a-5p, hsa-let-7c-3p, hsa-miR-30c-5p, hsa_circ_0059580, hsa_circ_0067475, hsa_circ_0002100 and hsa_circ_00072309 were consistent with the sequencing results. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to analyze the functions and roles of the differentially expressed miRNAs and circRNAs. The interaction maps between miRNAs and circRNAs were constructed, and signaling pathway maps were analyzed to determine the molecular mechanism and regulation of the differentially expressed miRNAs and circRNAs. Taken together, the results of the present study suggest that the Boyden chamber assay can be used to effectively isolate the somatic CBs and CPs of HCC, which can be used to screen the miRNAs and circRNAs associated with invasion and metastasis of HCC.     

沉默circRNA hsa_circ_0103809表达抑制胶质瘤细胞增殖和侵袭

目的:通过研究环状RNA(circRNA) hsa_circ_0103809对胶质瘤细胞增殖和侵袭的影响,来明确 hsa_circ_0103809在胶质瘤进展中所起到的作用。方法:采用qRT-PCR检测hsa_circ_0103809在胶质瘤细胞系U251和正常脑胶质细胞系HEB中表达水平的差异。U251细胞经小干涉RNA（siRNA）介导下调hsa_circ_0103809的表达水平后,采用qRT-PCR检测沉默效率。将U251细胞分为两组,即siRNA-circ沉默实验组和siRNA-NC阴性对照组,实验组转染hsa_circ_0103809的siRNA,对照组转染siRNA对照序列,后续分别使用CCK-8实验、EdU实验和Transwell实验检测hsa_circ_0103809的表达下调对U251细胞增殖和侵袭的影响。结果:hsa_circ_0103809在胶质瘤U251细胞中的表达水平明显高于正常脑胶质HEB细胞（P＜0.01）。在U251细胞中转染siRNA-hsa_circ_0103809后其表达水平明显降低（P＜0.01）。CCK-8实验结果显示,沉默hsa_circ_0103809的表达后,U251细胞在24 h和48 h的光密度（OD）值和对照组无明显差异,但实验组在72 h的OD值明显低于对照组（P＜0.01）。EdU实验结果显示,沉默hsa_circ_0103809的表达后,U251细胞的增殖能力较对照组明显降低（P＜0.01）。Transwell实验结果显示,沉默hsa_circ_0103809的表达后,U251细胞的侵袭能力较对照组明显降低（P＜0.001）。结论:hsa_circ_0103809在胶质瘤U251细胞中高表达,沉默hsa_circ_0103809表达可显著抑制胶质瘤细胞的增殖和侵袭。     

hsa_circ_0001741 promotes esophageal squamous cell carcinoma stemness,invasion and migration by sponging miR-491-5p to upregulate NOTCH3 expression

Circular RNAs (circRNAs) have been reported to play crucial roles in the progression of various cancers, including esophageal squamous cell carcinoma (ESCC). However, the function of circRNAs in ESCC stemness has not been reported. This study aimed to identify novel circRNAs that regulate ESCC stemness and explore their internal mechanisms in ESCC. We found that hsa_circ_0001741 was upregulated in ESCC tissues and was positively related to lymphatic metastasis, higher TNM stage, and poor prognosis. Functionally, hsa_circ_0001741 promoted ESCC cell stemness, invasion, and migration in vitro. Mechanistically, analysis of the relationship between hsa_circ_0001741 and tumor suppressor miR-491-5p revealed that hsa_circ_0001741 functioned as a miR-491-5p sponge. Specifically, hsa_circ_0001741 bound to miR-491-5p to prevent the microRNA from binding to the 3’-UTR of NOTCH3 mRNA and suppressing NOTCH3 expression. Moreover, the ablation of hsa_circ_0001741 significantly inhibited the tumorigenicity in vivo. In conclusion, hsa_circ_0001741 promotes ESCC stemness, invasion, and migration by sponging tumor suppressor miR-491-5p to upregulate NOTCH3 expression. Our findings identify a novel therapeutic target for ESCC patients and the expression level of hsa_circ_0001741 has the potential to serve as a prognostic biomarker for ESCC.     

Plasma circular RNA panel to diagnose hepatitis B virus-related hepatocellular carcinoma: A large-scale,multicenter study

To explore whether plasma circular RNAs (circRNAs) can diagnose hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), microarray and qPCR were used to identify plasma circRNAs that were increased in HBV-related HCC patients compared to controls (including healthy controls, chronic hepatitis B and HBV-related liver cirrhosis). A logistic regression model was constructed using a training set (n = 313) and then validated using another two independent sets (n = 306 and 526, respectively). Area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy. We identified a plasma circRNA panel (CircPanel) containing three circRNAs (hsa_circ_0000976, hsa_circ_0007750 and hsa_circ_0139897) that could detect HCC. CircPanel showed a higher accuracy than AFP (alpha-fetoprotein) to distinguish individuals with HCC from controls in all three sets (AUC, 0.863 [95% confidence interval, CI: 0.819–0.907] vs. 0.790 [0.738–0.842], p = 0.036 in training set; 0.843 [0.796–0.890] vs. 0.747 [0.691–0.804], p = 0.011 in validation set 1 and 0.864 [0.830–0.898] vs. 0.769 [0.728–0.810], p < 0.001 in validation set 2). CircPanel also performed well in detecting Small-HCC (solitary, ≤3 cm), AFP-negative HCC and AFP-negative Small-HCC.     

hsa＿circ＿0087378在食管鳞状细胞癌组织中的表达及其对患者预后的影响

目的：探讨hsa＿circ＿0087378在食管鳞状细胞癌（ESCC）组织中的表达及其对ESCC患者临床病理指标及预后的影响。方法：通过GEO数据库筛选ESCC组织及相应癌旁组织中差异表达的circRNA,采用qPCR法在10例ESCC组织及相应癌旁组织中进行验证。选取河北医科大学第四医院2015年10月至2016年4月收治的90例ESCC患者癌组织及配对癌旁组织标本,通过FISH法检测hsa＿circ＿0087378的表达,分析其表达与ESCC患者临床病理指标及预后的关系。结果：GEO数据库及qPCR分析结果显示,hsa＿circ＿0087378在ESCC组织中表达最明显。FISH结果显示,90例癌旁组织中21例hsa＿circ＿0087378呈高表达,高表达率为23.3%;90例ESCC组织中有65例hsa＿circ＿0087378呈高表达,高表达率为72.2%,癌组织中表达明显高于癌旁组织（P<0.05）。hsa＿circ＿0087378在ESCC组织中的高表达与患者的临床分期和淋巴结转移有关联（P<0.05）,hsa＿circ＿0087378高表达组ESCC患者的5...     

GEO数据库分析环状RNA在乳腺癌组织中的表达

目的采用生物信息学方法探究环状RNA在乳腺癌侵袭转移中的作用。方法从GEO数据库中检索并下载乳腺癌组织的芯片数据,利用R软件对4对Luminal A型乳腺癌组织和4对三阴性乳腺癌组织进行差异分析,然后利用miRanda 软件和 RNAhybrid 软件对差异表达的环状RNA和miRNAs之间的相互作用进行预测。通过TCGA数据库分析环状RNA的亲本基因在乳腺癌组织中的表达。结果通过差异分析,发现共有27个环状RNA异常表达,其中6个环状RNA上调,21个环状RNA下调;通过软件预测构建了环状RNA／miRNAs相互作用网络;通过基因表达分析,hsa_circ_0000965、hsa_circ_0000523、hsa_circ_0044234、hsa_circ_0058451和hsa_circ_0091994可能正向调控其亲本基因的表达。结论在三阴性乳腺癌组织中的异常表达环状RNA将可能成为乳腺癌转移治疗的新靶点。     

has_circ_0070512 promotes prostate cancer progression by regulating the miR-338-3p/hedgehog signaling pathway

Circular RNAs (circRNAs) are a type of non-coding RNA that plays a vital role in biology. circRNAs appear to have a role in the development and progression of several malignancies, according to research. However, circRNAs that regulate prostate cancer (PCa) progression are still largely unknown and deserve further exploration. The aim of this study was to investigate the effect of hsa_circ_0070512 on the function and mechanism of PCa. hsa_circ_0070512 was increased in PCa tissues and cells and was mostly found in the cytoplasm of PCa cells. Overexpression of hsa_circ_0070512 considerably increased PCa cell proliferation and migration, whereas silencing of hsa_circ_0070512 greatly decreased PCa cell proliferation and migration. Mechanistically, we show that hsa_circ_0070512 acts as a “molecular sponge” for miR-338-3p and that the miR-338-3p mimics partially block the pro-tumor effects of hsa_circ_0070512. RNA sequencing analysis of PC3 cells stably overexpressing hsa_circ_0070512 revealed that hedgehog was downstream of the signaling pathways of hsa_circ_0070512 and miR-338-3p. Our results implied that hsa_circ_0070512 regulated the hedgehog signaling pathways through miR-338-3p to enhance PCa growth and migration, providing a new diagnostic and therapeutic target for PCa.     

环状RNA hsa_circ_0005320在三阴性乳腺癌中的表达及其对细胞增殖的影响

背景与目的：环状RNA（circular RNA,circRNA）作为新近发现的具有重要调节潜能的非编码RNA,与多种肿瘤的发生、发展密切相关,但在三阴性乳腺癌（triple-negative breast cancer,TNBC）中罕见报道。探讨hsa_circ_0005320在TNBC中的表达变化及其对细胞增殖的影响。方法：通过RNA测序（RNA sequencing,RNA-Seq）分析4对于重庆医科大学附属第一医院行手术切除的TNBC组织和癌旁组织,采用实时荧光定量聚合酶链反应（real-time fluorescent quantitative polymerase chain reaction,RTFQ-PCR）验证20对TNBC组织和癌旁组织以及正常人乳腺上皮细胞MCF-10A和TNBC细胞MDA-MB-231、BT-549中hsa_circ_0005320的表达,并通过荧光原位杂交（fluorescence in situ hybridization,FISH）实验进一步检测其在TNBC中的表达。采用RTFQ-PCR检测沉默hsa_circ_0005320后TNBC细胞中hsa_circ_0005320的表达;采用细胞计数试剂盒（cell counting kit-8,CCK-8）和EdU实验检测细胞增殖;采用流式细胞术、Hoechst 33342、Tunel实验检测细胞凋亡;采用流式细胞术检测细胞周期;采用蛋白质印迹法（Western blot）检测LIF-STAT3通路相关蛋白的表达情况。结果：hsa_circ_0005320在TNBC组织和细胞中显著高表达;在TNBC细胞中沉默hsa_circ_0005320后其表达量显著降低,细胞增殖能力下降,促进细胞凋亡,导致细胞周期阻滞在G₁期,且LIF、P-STAT3蛋白表达水平明显下降。结论：hsa_circ_0005320在TNBC中高表达,沉默hsa_circ_0005320可显著抑制TNBC细胞的增殖,诱导细胞凋亡。     

Upregulated hsa_circ_0000129 expression promotes proliferation and migration of breast cancer cells

Circular RNAs (circRNAs) are considered potential biomarkers in the pathogenesis and detection of several types of cancer. The present study aimed to investigate the role of hsa_circ_0000129 in the pathogenesis and molecular mechanism underlying breast cancer. A total of 68 pairs of breast cancer and corresponding paracancerous tissue samples, three different breast cancer cell lines (MCF-7, MDA-MB-231 and MDA-MB-468) and a normal human breast cell line (MCF-10A) were used to investigate the expression of hsa_circ_0000129. The effect of hsa_circ_0000129 on cell proliferation, migration and colony formation was assessed in MCF-7 and MDA-MB-468 cells, along with the expression of enhancer of zeste homolog 2 (EZH2). The results demonstrated that hsa_circ_0000129 expression was significantly higher in breast cancer tissues compared with normal tissues. In addition, high hsa_circ_0000129 expression was significantly associated with lymph node metastasis and a higher tumor-node-metastasis stage. Comparisons between the breast cancer cell lines (MCF-7, MDA-MB-231 and MDA-MB-468) and MCF-10A cells indicated similar results. MCF-7 cells overexpressed with hsa_circ_0000129 significantly increased cell proliferation, migration and colony formation compared with the negative control group, the effects of which were reversed following hsa_circ_0000129 knockdown in MDA-MB-468 cells. Furthermore, EZH2 expression was positively associated with hsa_circ_0000129 expression. Taken together, the results of the present study suggest that hsa_circ_0000129 may represent a promising prognostic biomarker for breast cancer. In addition, the role of hsa_circ_0000129 in breast cancer cell lines indicates a mechanism for tumorigenesis, as well as a potent target for the treatment of malignant progression.     

hsa＿circ＿0140180通过调控miR-1287-5p影响食管鳞状细胞癌细胞的迁移及侵袭能力

目的：探讨hsa＿circ＿0140180在食管鳞状细胞癌（ESCC）细胞中的表达水平及对其细胞恶性生物学行为的影响与分子机制。方法：收集2018年11月至2019年3月间在南充市中心医院胸心外科手术切除的6对ESCC组织和对应癌旁组织并进行全转录组测序,筛选出在ESCC组织中低表达的hsa＿circ＿0140180;建立过表达hsa＿circ＿0140180的TE-1和KYSE30细胞,qPCR法检测hsa＿circ＿0140180在人正常食管上皮细胞、ESCC细胞中的表达,以及过表达hsa＿circ＿0140180后TE-1和KYSE30细胞中miR-1287-5p的表达;CCK-8法和FCM检测过表达hsa＿circ＿0140180对TE-1和KYSE30细胞增殖和周期的影响;划痕实验和Transwell实验检测过表达hsa＿circ＿0140180对TE-1和KYSE30细胞迁移和侵袭能力的影响,双荧光素酶报告实验验证hsa＿circ＿0140180与miR-1287-5p的靶向关系。WB法检测过表达hsa＿circ＿0140180对TE-1和KYSE30细胞中EMT相关蛋白的...     

环状RNA hsa_circ_0050900在乳腺癌中的表达及其对细胞生物学行为影响的机制研究

背景与目的：环状RNA（circular RNA,circRNA）作为特殊的非编码RNA,一般在体内低表达,且不易被RNA酶降解,结构与表达稳定。随着测序技术的进步,目前发现多种肿瘤的发生、发展与circRNA有关。但未见乳腺癌的发生、发展与hsa_circ_0050900的异常表达相关的报道。探究乳腺癌组织中hsa_circ_0050900的表达以及其影响乳腺癌细胞生物学行为的机制。方法：选取在重庆医科大学附属第一医院经手术切除的4例女性乳腺癌组织及对应的癌旁组织,采用RNA测序（RNA sequencing,RNA-seq）进行测序分析,并运用实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR）验证乳腺癌中hsa_circ_0050900的表达,其中包括30例乳腺癌组织及其癌旁组织（在重庆医科大学附属第一医院经手术切除）,正常人乳腺上皮细胞系即对照组细胞MCF-10A以及两种乳腺癌细胞系MCF-7、SK-BR-3。通过RTFQ-PCR验证乳腺癌细胞中hsa_circ_0050900被敲低后的表达水平;分别运用细胞计数试剂盒（cell counting kit-8,CCK-8）实验和EdU实验、细胞划痕实验、transwell实验验证细胞的增殖、迁移功能;细胞凋亡的检测采用TUNEL一步法;Hoechst 33342实验通过对细胞核染色确定细胞的状态以检测细胞凋亡;细胞周期蛋白D2（cyclin D2,CCND2）和周期蛋白依赖性激酶4（cyclin-dependent kinase 4,CDK4）的表达采用蛋白质印迹法（Western blot）检测。结果：根据测序结果,挑选出在乳腺癌和细胞中明显高表达的circRNA hsa_circ_0050900;构建的si-circ质粒可显著降低hsa_circ_0050900在乳腺癌细胞中的表达,转染si-circ的细胞增殖能力降低,并促进细胞凋亡,且降低细胞周期相关蛋白CCND2、CDK4的表达。结论：circRNA hsa_circ_0050900在乳腺癌组织中的表达显著高于癌旁组织,敲低hsa_circ_0050900对细胞增殖、迁移能力、细胞凋亡及细胞周期有调控作用。     

hsacirc0006867在结直肠癌组织中的表达及其临床意义北大核心

目的:探讨环状RNA hsa_circ_0006867在结直肠癌中的表达及其与临床病理因素的关系。方法:全转录组测序筛选结直肠癌中特异circRNAs表达谱,挑选出差异表达显著的hsa_circ_0006867,qRT-PCR检测54例结直肠癌组织及癌旁组织中hsa_circ_0006867表达情况,分析其表达水平与结直肠癌临床病理特征的相关性,ROC曲线分析hsa_circ_0006867在结直肠癌中的诊断价值。结果:测序获得circRNAs在结直肠癌中的差异表达谱,qRT-PCR验证hsa_circ_0006867在结直肠癌中表达下调(P<0.05)。其表达水平与肿瘤分化程度和远处转移有关(P<0.05)。ROC曲线显示hsa_circ_0006867诊断结直肠癌AUC为0.851(95%CI:0.775~0.927),当截断值为0.0146时,敏感度为88.46%(95%CI:0.770~0.946),特异度为73.08%(95%CI:0.598~0.832),差异具有统计学意义(P<0.001)。结论:hsa_circ_0006867在结直肠癌中表达下调,与相关临床病理特征密切联系,可作为潜在结直肠癌临床诊断指标。     

Hsa_circ_0003998 Promotes Chemoresistance via Modulation of miR-326 in Lung Adenocarcinoma Cells

Circular RNAs (circRNAs) represent a new class of noncoding RNAs that is involved in the development of cancer. However, little is known about their role in chemoresistance. In the present study, we found that hsa_circ_0003998 expression levels in lung adenocarcinoma (LAD) tissues and docetaxel-resistant cell lines (A549/DTX and H1299/DTX) were upregulated. Knockdown of hsa_circ_0003998 decreased chemoresistance, inhibited proliferation, and enhanced apoptosis in docetaxel-resistant LAD cells. Moreover, by using bioinformatics and luciferase reporter assays, we found that miR-326 was a direct target of hsa_circ_0003998. Functional analysis revealed that miR-326 mediated the effect of hsa_circ_0003998 on chemosensitivity. Our findings provide a molecular insight on understanding drug resistance in LAD cells. Therefore, inactivation of hsa_circ_0003998 or activation of miR-326 could be a novel approach for the treatment of LAD.     

Circular RNA hsa_circ_001895 serves as a sponge of microRNA‐296‐5p to promote clear cell renal cell carcinoma progression by regulating SOX12

There is an urgent need to find novel potential therapeutic targets for the diagnosis and treatment of clear cell renal cell carcinoma (ccRCC) due to its highly invasive ability as a common urological malignant tumor. Circular RNAs (circRNAs) have been indicated as potentially critical mediators in various types of tumor progression. We first used qRT‐PCR analysis to find dysregulated circRNAs in ccRCC. A novel circRNA, hsa_circ_001895, was upregulated in ccRCC specimens and associated with metastatic properties of ccRCC. However, the tumorigenic mechanism of hsa_circ_001895 on ccRCC is yet to be found. We first indicated that hsa_circ_001895 predicted a poor prognosis in ccRCC patients. Additionally, overexpression of hsa_circ_001895 not only promoted cell proliferation, invasion and migration of ccRCC, but also inhibited cell apoptosis, whereas hsa_circ_001895 knockdown reversed the effect on ccRCC progression. In vivo s.c. xenotransplanted tumor model also showed that silencing hsa_circ_001895 could suppress in vivo ccRCC growth. Mechanistically, hsa_circ_001895 directly binds with microRNA (miR)‐296‐5p and inhibits its expression. Moreover, sex determining region Y (SRY)‐box 12 (SOX12) was identified as a target of miR‐296‐5p, the expression of which was suppressed by miR‐296‐5p. Notably, the inhibitory effect of hsa_circ_001895 on ccRCC progression was reversed by miR‐296‐5p inhibitor. In general, our findings indicated that hsa_circ_001895 may sponge miR‐296‐5p and promote SOX12 expression, which is the underlying mechanism of hsa_circ_001895‐induced ccRCC progression.     

环状RNA hsa_circ_0058514在三阴性乳腺癌中的表达及作用研究

背景与目的：环状RNA（circular RNA，circRNA）是一类具有重要调节潜能的非编码RNA，参与多种肿瘤的发生、发展，但对于三阴性乳腺癌（triple-negative breast cancer，TNBC）尚未见报道。该研究旨在探讨环状RNA hsa_circ_0058514在TNBC发生、发展中的作用。方法：采用RNA测序（RNA sequencing，RNA-seq）对4对TNBC组织和癌旁组织进行分析，采用实时荧光定量聚合酶链反应（real-time fluorescent quantitative polymerase chain reaction，RTFQ-PCR） 对20对TNBC组织和癌旁组织以及正常人乳腺上皮细胞MCF-10A和TNBC细胞MDA-MB-231和BT-549中hsa_circ_0058514的表达进行验证。将干扰质粒pLL3.7-sh-circ转染TNBC细胞MDA-MB-231和BT-549后，采用RTFQ-PCR检测细胞中hsa_circ_0058514的表达；采用细胞计数试剂盒（cell counting kit-8，CCK-8）和EdU实验检测细胞增殖；采用划痕和Transwell小室实验分别检测细胞迁移和侵袭；采用流式细胞术检测细胞凋亡和细胞周期；采用蛋白质印迹法（Western blot）检测细胞周期蛋白E1（cyclin E1，CCNE1）和细胞周期蛋白依赖性激酶2（cyclin-dependent kinase 2，CDK2）蛋白的表达。结果：环状RNA hsa_circ_0058514在TNBC组织和细胞中显著高表达（P<0.001，P<0.01）；转染pLL3.7-sh-circ后，TNBC细胞中hsa_circ_0058514的表达量显著低于对照组（P<0.001）。下调hsa_circ_0058514后，TNBC细胞增殖、迁移及侵袭能力下降，并促进细胞凋亡，导致细胞周期阻滞。Western blot结果显示，转染pLL3.7-sh-circ后，CCNE1和CDK2蛋白的表达下调（P<0.05）。结论：环状RNA hsa_circ_0058514在TNBC组织和细胞中均高表达，其在TNBC发生、发展中可能起到癌基因的作用，并有望成为TNBC治疗的新靶点。     

hsa_circ_0064369在食管鳞癌组织和细胞中的表达及临床意义

目的:分析hsa_circ_0064369在食管鳞癌中的表达及其临床意义。方法:采用高通量芯片技术检测6例食管鳞癌患者癌组织和对应癌旁组织中差异表达circRNA,依据差异倍数≥5、P＜0.01、样本间一致性高、原始信号值相对高等这几个特征挑选出下调8.4倍的hsa_circ_0064369为研究对象。运用实时荧光定量PCR（real-time quantitative polymerase chain reaction,RT-qPCR）检测并比较43对食管鳞癌组织和对应癌旁组织及正常食管上皮细胞（NE6）和两种食管鳞癌细胞（NE3、KYSE9706）中hsa_circ_0064369的表达水平。分析hsa_circ_0064369表达水平与临床病理特征的关系;Kaplan-Meier法分析hsa_circ_0064369表达水平与生存期的关系;构建受试者工作特征（receiver operating characteristic,ROC）曲线评估hsa_circ_0064369对食管鳞癌诊断的灵敏度和特异性。结果:食管鳞癌组织中hsa_circ_0064369相对表达量（0.35±0.41）低于癌旁正常组织（1.88±1.22）（P＜0.01）;两种食管癌细胞中hsa_circ_0064369的表达水平（0.46±0.18,0.53±0.09）均低于正常食管上皮细胞（1.00±0.37）（P＜0.05）。hsa_circ_0064369表达下调与TNM分期显著相关（P=0.022）,与性别、年龄、分化程度、肿瘤最大径、肿瘤位置、淋巴结转移无统计学相关（P＞0.05）。hsa_circ_0064369低表达食管鳞癌患者无进展生存期较高表达患者短;ROC曲线下面积为0.928,当截断值为0.699 5时其诊断灵敏度和特异性为88.4%和90.7%。结论:hsa_circ_0064369在食管鳞癌中表达量下降,其低表达提示预后不良,是潜在的食管癌诊断和治疗的新型分子标志物。