维吾尔族百岁老人唾液IgA、IgG、IgM及C3、C4含量检测报告

目的本旨在研究维吾尔族百岁老人及维吾尔族老年人唾液中免疫球蛋白和补体的含量关系，探索长寿因素．方法用上海长征医学科学有限公司生产的免疫球蛋白及补体试剂盒，于340nm，用分光光度计，对23名百岁老人和21名老人的唾液进行检测．结果百岁组唾液免疫球蛋白A、G．M，及补体C3、C4各均数、都高于老人组，除唾液IgA、有显着性差异(P<0．05)外，余无差别(P>0．05)．结论提示百岁老人唾液免疫球蛋白及补体．维持在相当于老年水平，可能为长寿因素之一．     

血α_1、α_2、β、γ球蛋白及κ、λ轻链在儿童支原体肺炎的意义

目的通过对肺炎支原体肺炎(MPP)儿童患者的血清中α_1、α_2、β、γ球蛋白及免疫球蛋白κ轻链、λ轻链含量结果进行统计分析,探讨血α_1、α_2、β、γ球蛋白及κ、λ轻链在MPP发病中的意义。方法选取我院儿科2016年1月到2017年1月期间收治的MPP儿童患者120例为研究对象,作为MPP组,同时选取120例来我院健康儿童作为对照组,分别检测两组儿童血清中α_1球蛋白区带(α_1酸性糖蛋白、α_1抗胰蛋白酶、α_1脂蛋白、甲胎蛋白)、α_2球蛋白区带(结合珠蛋白、α_2巨球蛋白、铜蓝蛋白)、β球蛋白区带(转铁蛋白、β脂蛋白、补体C_3、f纤溶酶原)γ球蛋白区带(Ig G、Ig M、Ig A、Ig D、Ig E及C反应蛋白)以及κ轻链、λ轻链的含量。结果 (1)α_1球蛋白区带,与对照相比,MPP组α_1酸性糖蛋白和α_1抗胰蛋白酶均升高,但α_1抗胰蛋白酶升高的更显著(P0.05);α_1脂蛋白和甲胎蛋白均降低,但两组之间无统计学意义(P0.05);(2)α_2球蛋白区带,与对照组相比,MPP组结合珠蛋白、α_2巨球蛋白和铜蓝蛋白均升高,但两组之间只有结合珠蛋白差异具有统计学意义(P0.05);(3)β球蛋白区带,与对照组相比,转铁蛋白和f溶酶原降低,且两组之间无统计学意义(P0.05)。β脂蛋白和补体C_3均升高,且两组之间补体C_3差异具有统计学意义(P0.05)。(4)γ球蛋白区带,与对照组相比,MMP组Ig G、Ig M、Ig D、CRP、Ig E均明显升高,但Ig G、Ig M、CRP升高的更显著(P0.05),Ig A明显降低(P0.05);(5)与对照组相比,MPP组κ轻链、λ轻链均升高,但差异不具有统计学意义(P0.05)。结论 MPP儿童患者的血α_1、α_2、β、γ球蛋白的变化可作为支原体肺炎的辅助诊断。     

血清蛋白电泳、免疫球蛋白及其轻链测定对肝病患者的临床意义

目的探讨血清中免疫球蛋白和轻链测定以及血清蛋白电泳在肝病患者中的临床应用价值。方法用免疫散射比浊法在特定蛋白分析仪上检测92例肝病患者(包括急性肝炎患者28例、慢性肝炎患者33例、肝硬化患者31例)及45例健康者血清中免疫球蛋白IgG、IgA、IgM以及κ轻链和λ轻链的水平;用琼脂糖凝胶电泳法对所有样本进行血清蛋白电泳检测。结果与对照组相比,急性肝炎组以IgM升高为主,差异有统计学意义(P<0.05);两组κ轻链和λ轻链水平比较,差异无统计学意义(P>0.05)。急性肝炎组和肝硬化组IgG、IgA水平均高于对照组,差异有统计学意义(P<0.05);两组κ轻链和λ轻链水平分别与对照组比较,差异均有统计学意义(P<0.05)。血清蛋白电泳结果显示,急性肝炎组与对照组相比,两组γ区球蛋白含量比较,差异无统计学意义(P>0.05);慢性肝炎组和肝硬化组γ区球蛋白含量与对照组比较,差异有统计学意义(P<0.05)。结论血清中免疫球蛋白及其轻链的含量与肝脏疾病密切相关,慢性肝炎肝硬化患者血清蛋白电泳呈现典型的多克隆增殖图谱。血清蛋白电泳、免疫球蛋白和轻链水平的测定可作为肝脏功能监测的辅助指标。     

肾功能对慢性肾脏病血清游离轻链的影响

背景:血清游离轻链在单克隆免疫球蛋白病的诊断中有重要价值,目前其参考范围主要依据国外文献,缺乏中国大样本数据,随肾功能不全进展,κ/λ比值如何变化及其意义存在争议。目的:探索慢性肾脏病(CKD)患者血清游离轻链(FLC)的水平、参考范围及对单克隆免疫球蛋白病的诊断价值。方法:东部战区总医院国家肾脏病临床医学研究中心住院并检测血清游离轻链的患者共2 711例,对其临床资料进行回顾性分析。结果:血清κFLC、λFLC与血清肌酐、胱抑素C呈正相关,与估算的肾小球滤过率(eGFR)呈负相关;κ/λ存在相同的相关趋势,但与eGFR相关性较强(均P0.01)。肾功能正常并除外单克隆免疫球蛋白病及自身免疫病、感染的CKD患者中(n=690),κFLC范围为10.75～68.22 mg/L,λFLC范围为12.16～50.29 mg/L,κ/λ范围为0.49～1.93(均为95%置信区间)。扩大至所有肾功能分期后,κFLC范围为11.10～152.89 mg/L,λFLC范围为12.20～118.53 mg/L,κ/λ范围为0.52～2.36。当按肾功能分层使用不同参考范围时,可提高诊断效率。结论:随肾功能不全进展,血清FLC水平及κ/λ比值升高,影响对单克隆球蛋白病的诊断效率,不同的肾功能状态CKD患者应选择不同的正常参考值范围。     

老年慢性心力衰竭早期肾损害患者血清轻链蛋白、胱抑素C的浓度及临床意义

目的 探究老年慢性心力衰竭早期肾损害患者血清免疫球蛋白游离轻链（free light chain,FLC）、胱抑素C(cystatin C,Cys-C)的浓度及临床意义。方法 选取2016年12月至2019年12月于航天中心医院就诊的老年慢性心力衰竭早期肾损害患者80例作为观察组,另选择同期于航天中心医院进行体检的健康人群80名作为对照组。采用免疫散射比浊法定量测定两组血清轻链蛋白FLCκ、FLCλ浓度;采用免疫透射比浊法检测血清Cys-C浓度;采用Logistic回归分析影响老年慢性心力衰竭早期肾损害发生的危险因素;采用受试者工作特征曲线（receiver operating characteristic curve,ROC）分析血清轻链蛋白FLCκ、FLCλ、Cys-C对老年慢性心力衰竭早期肾损害的诊断价值。结果 观察组血清FLCκ、FLCλ及Cys-C[(82.86±28.15)mg/L、（44.46±14.04）mg/L、（1.80±0.49）mg/L]均显著高于对照组[（49.09±15.62）mg/L、（29.09±8.02）mg/L、（0.97±0.26）mg/L],差异...     

乙型肝炎患者血清免疫球蛋白和补体C3/C4检测的意义

目的探讨检测慢性乙型肝炎、乙型肝炎肝硬化和乙型肝炎肝衰竭患者血清免疫球蛋白(Ig G、Ig M、Ig A)和补体C3、C4水平的意义。方法在32例慢性乙型肝炎、33例乙型肝炎肝硬化和35例乙型肝炎肝衰竭患者,采用速率散射免疫比浊法检测血清免疫球蛋白(Ig G、Ig M、Ig A)和补体C3、C4。结果乙型肝炎肝衰竭患者血清Ig A水平为(4.06±1.20)mg/L,显著高于慢性乙型肝炎[(3.18±1.23)mg/L,P0.05]和乙型肝炎肝硬化患者[(3.21±1.53)mg/L,P0.05];C3水平为(0.33±0.02)g/L,显著低于慢性乙型肝炎[(0.54±0.25)g/L,P0.05]和肝硬化患者[(0.60±0.05)g/L,P0.05];三组其他指标无显著性相差(P0.05)。结论血清Ig G、Ig M、Ig A和补体C3、C4水平检测对肝脏疾病的诊断有一定的临床意义。     

伴单克隆IgG1沉积的增生性肾小球肾炎

61岁女性,病程10个月,临床表现尿检异常、低补体血症伴贫血、血游离轻链比值异常,免疫固定电泳见Ig G-λ单克隆免疫球蛋白条带,肾脏体积增大,骨髓浆细胞7. 5%、未见原始和幼稚浆细胞,肾活检示膜增生性肾小球肾炎(结节样病变),Ig G1++、λ轻链+颗粒状沉积于系膜区及血管袢,Ig G2、Ig G3、Ig G4、κ轻链阴性;诊断为伴单克隆Ig G1沉积的增生性肾小球肾炎。给予沙利度胺联合地塞米松治疗1年,蛋白尿完全缓解,补体上升,血游离轻链比值恢复正常,血清M蛋白转阴。     

姜黄素在大鼠重症急性胰腺炎中的研究

目的观察姜黄素对大鼠重症急性胰腺炎(SAP)NF-κB p65蛋白的表达水平,血清淀粉酶、细胞因子及胰腺组织的改变,探讨姜黄素对大鼠重症急性胰腺炎防治的作用。方法通过牛磺胆酸钠诱导大鼠重症急性胰腺炎模型,将54只SD大鼠随机分成3组:假手术对照组(N组)18只,重症急性胰腺炎模型组(M组)18只,姜黄素干预组(C组)18只。3组分别于3h、6h、12h等3个时间点取腹水、血清及胰腺组织。采用全自动生化分析仪检测各组血清淀粉酶(AMY)水平。采用ELISA检测各组血清IL-6和TNF-α的水平。采用HE染色观察胰腺的病理学损伤和评分及免疫组化染色测定NF-κBp65蛋白的表达。结果 M组血清AMY较N组明显升高(P<0.0001);C组血清AMY水平较M组有所下降(P<0.05)。C组的水肿、炎症浸润、出血和Schmidt评分均较M组降低(P<0.05)。M组大鼠各时间点的胰腺组织NFκb的阳性表达水平较N组和C组明显增加(P<0.05),C组各时间点胰腺组织NFκB p65蛋白的阳性表达较M组有所下降(P<0.05)。M组血清IL-6、TNF-α水平比N组明显升高(P<0.05),C组工IL-6、TNF-α水平较M组明显下降(P<0.05)。结论姜黄素能在一定程度上抑制NF-κB信号通路的激活并减轻SAP的病理损伤,从而起到防治作用。     

糖尿病早期肾损害患者尿糖及血清Cys-C、游离轻链κ/λ比值的变化及临床意义

目的 分析糖尿病（diabetes mellitus, DM）早期肾损害患者尿糖及血清胱抑素C(Cys-C)、游离轻链κ/λ比值的变化及临床意义。方法 回顾性分析2020年11月—2022年11月在福建省龙岩人民医院进行治疗的172例2型糖尿病患者的临床资料,根据患者尿蛋白排泄率（UAER）将其分为DM组（n=94,UAER<30 mg/d,不存在早期肾损伤）,早期DN组（n=78,UAER 30～300 mg/d,存在早期肾损伤）。比较两组患者尿糖、血清Cys-C及游离轻链κ、游离轻链λ及游离轻链κ/λ比值水平,并分析以上指标与早期DN的相关性。结果 早期DN组患者尿糖水平、血清Cys-C、游离轻链κ、游离轻链λ以及游离轻链κ/λ比值均高于DM组患者,差异有统计学意义（P<0.05）;尿糖、血清Cys-C及血清游离轻链κ/λ比值异常增高均为DM早期肾损害的危险因素（P<0.05）;血清游离轻链κ/λ比值与尿糖水平及血清Cys-C水平均呈正相关（r=0.366、0.341,P<0.05）;受试者工作特征（ROC）曲线分析显示,尿糖预测早期DN曲线下面积为0.75...     

大黄对高血压脑出血术后患者血清补体C3、C4的影响

目的探讨大黄对高血压脑出血(HICH)开颅血肿清除术后患者血清补体C3、C4含量的影响。方法按随机对照方法将40例HICH开颅血肿清除术后患者分为两组。术后4d开始对照组给予西医常规治疗;治疗组在西医常规治疗基础上加用生大黄粉5g～10g加入温开水40mL溶解后,口服或鼻饲(2～3)次/日。于术后7d、14d免疫透射比浊法测量,比较两组血清补体C3、C4含量。结果治疗组治疗后7d、14d时血清补体C3含量(1.36±0.30)g/L、(1.20±0.26)g/L,C4含量(0.31±0.07)g/L、(0.28±0.03)g/L,低于对照组(P<0.05)。结论大黄可显著降低HICH术后患者血清补体C3、C4含量。     

烟曲霉致敏的呼吸道疾病患者对其他真菌致敏的sIgE水平及相关性分析

目的 探讨呼吸道疾患者对真菌致敏的免疫球蛋白E (specific ImmunoglobulinE,sIgE)水平及多重致敏现象.方法 筛选广州医科大学附属第一医院烟曲霉sIgE阳性且级别大于等于三级的哮喘或变态反应性支气管肺曲霉菌病(ABPA)成人患者,分为烟曲霉致敏哮喘组及ABPA组.采用ImmunoCAP 1000荧光酶联免疫系统检测患者血清点青霉、分支孢霉、烟曲霉、白假丝酵母霉、链格孢霉与长蠕孢霉sIgE浓度.结果 ABPA患者烟曲霉sIgE显著高于烟曲霉致敏哮喘患者(P＜0.05)[26.7 (13.3,54.3) kU/L vs 12.7 (5.90,33.4) kU/L]、白假丝酵母sIgE[7.90 (1.40,6.00) kU/L vs 1.00 (0.40,6.00) kU/L]、点青霉sIgE[17.6 (6.80,37.6) kU/L vs 4.30 (4.30,4.30) kU/L]、链格孢sIgE[2.60 (1.70,16.0)kU/L vs 0.90 (0.40,2.10) kU/L].所有患者皆有多重霉菌致敏现象,各霉菌sIgE水平存在不同程度相关,最优尺度分析显示烟曲霉与链格孢霉关系最近(Cronbach's Alpha=95.7％).结论烟曲霉菌致敏患者常伴多重霉菌过敏现象,或因霉菌间分泌相同的致敏蛋白引起,多种霉菌sIgE浓度的检测或可作为真菌致敏哮喘辅助检查.     

人血丙种球蛋白治疗围产期心肌病的疗效评价

目的探讨应用人血丙种球蛋白治疗产后围产期心肌病的疗效评价。方法选取浙江省湖州市中心医院确诊的产后围产期心肌病患者49例,分为治疗组和对照组。治疗组在标准化治疗基础上加用人血丙种球蛋白治疗,用法为400mg／（kg·d）连续静脉注射5d。利用超声心动图对两组治疗前、后的左心室舒张末期内径（1eftventricularenddiastolicdimension,LVEDd）及左心室射血分数（1eftventricularejectionfraction,LVEF）进行测定,并比较两组治疗前、后免疫球蛋白G、A、M浓度。结果治疗组治疗后LVEDd、LVEF较治疗前有明显改善,差异有统计学意义[（5.8±0．4）mm眠（6．2±0．7）nlm,P〈O．05;58％±13％眠39％±1l％,P〈0．05];对照组仅LVEF改善,差异有统计学意义（52％±6％VS．40％±7％,P〈O．05）。治疗组治疗后LYEDd、LVEF改善效果优于对照组治疗后,差异有统计学意义[（5．8±0．4）mm us．（6．0±0．2）mm,P〈0．05;58％±13％眠52％±6％,P〈0．05]。治疗组及对照组治疗后血清免疫球蛋白G、A、M浓度均较治疗前有下降,其中血清免疫球蛋白G浓度在治疗组较对照组下降更为明显,差异有统计学意义[（9．58±1．14）g／L强（10．98±1．16）g／L,P〈O．05]。结论围产期心肌病在传统治疗基础上采用大剂量人血丙种球蛋白治疗,可取得明显疗效,且LVEDd、LVEF及免疫球蛋白G的测定可作为其有效的评价指标。     

Immunoglobulin E production in chronic pancreatitis

OBJECTIVES: Serum immunoglobulin E (IgE) was investigated in patients with chronic pancreatitis in order to elucidate possible disease mechanisms linking pancreatitis, adverse reaction to foods and allergy. DESIGN AND METHODS: Serum IgE was analysed in 86 non-atopic patients with advanced chronic pancreatitis and 54 non-atopic controls. IgE detection was performed by enzyme-linked immunofluorescence. RESULTS: Mean IgE levels (+/- standard error of mean) in chronic pancreatitis patients (286.1+/-49 kU/l) were found to be significantly elevated compared to controls (67.7+/-11 kU/l; P<0.0001). Normal IgE levels (<100 kU/l) were present in 40/54 control patients (74.1%), but only 39/86 pancreatitis patients (45.3%). Of the patients with chronic pancreatitis, 47/86 (54.6%) had clearly elevated IgE levels of >100 kU/l and their IgE values did not show a Gaussian distribution. However, nine-fold higher IgE levels were found in chronic pancreatitis patients with alcohol consumption of >25 g/day and exocrine insufficiency (915.5+/-240 kU/l) than in pancreatitis patients with normal exocrine function and no alcohol consumption (103.4+/-43 kU/l; P<0.001). Moreover, acute episodes of chronic pancreatitis were found to increase serum IgE levels. CONCLUSIONS: In patients with chronic pancreatitis, serum IgE production is markedly enhanced, especially during acute inflammatory episodes or when alcohol is consumed. Since abstinence from alcohol and pancreatic enzyme substitution are associated with clearly lower IgE levels, it may be concluded that pancreatic insufficiency with reduced nutrient digestion and alcohol consumption stimulate IgE production. This finding gives rise to the speculation that, apart from pancreatic inflammation, cross-linking of IgE with alimentary or other antigens might be involved in the pathophysiology of a sub-population of patients with chronic pancreatitis and manifest pancreatic insufficiency.     

Serum IgE Levels in Adults with Asthma

Serum total immunoglobulin E (IgE) was measured in 88 normal adults and 100 adults with asthma using Phadebas IgE PRIST kits. The geometric mean IgE in our normal subjects was found to be higher than that reported by some investigators but was similar to or lower than that reported by others without showing any geographic pattern. It increased progressively from 92 kU/L in normal controls through 205 kU/L in cases of asthma with low atopic scores to 464 kU/L in asthma cases with high atopic scores. There was wide variation in IgE levels among individuals, making it difficult to use it to classify any subject into one of these groups. However, as a group, female asthmatics had lower mean IgE levels (182 kU/L) than did men (577 kU/L), and a higher proportion of them had low atopic scores (60% of the women versus 47% of the men). This suggests that probably more women had intrinsic asthma, while extrinsic asthma was more common among men in the population studied.     

Personality and longevity: findings from the Georgia Centenarian Study

Centenarians are thought of as unique and exceptional survivors. This study evaluated specific personality traits and configurations of traits among participants of the Georgia Centenarian Study. Two hundred and eighty five centenarians and their nominated proxies participated in this study. Self ratings and proxy informant ratings were obtained for different traits and facets of the Big-5 personality typology. Results suggested that centenarians overall had low levels of Neuroticism, but high levels of Extraversion, Competence, and Trust. When compared to centenarian self ratings, proxies provided significantly higher ratings for Neuroticism, Hostility, and Vulnerability, but lower ratings for Competence and Trust. Among Centenarians, the personality configuration of low Neuroticism, high Competence, and high Extraversion traits is over-represented relative to chance. The results confirm that centenarians show several unique single traits, but that a special combination of traits (i.e., low levels of Neuroticism, high Competence, and high Extraversion) are also notable in this group of exceptional survivors. Additional authors include S.M. Jazwinski, R.C. Green, M. Gearing, W.R. Markesbery, J.L. Woodard, M.A. Johnson, J.S. Tenover, W.L. Rodgers, D.B. Hausman, C. Rott, and J. Arnold.     

青海省兴海县大骨节病病区儿童血清丙二醛和抗氧化酶水平观察

目的 观察青海省兴海县大骨节病(KBD)病区儿童血清中丙二醛(MDA)及抗氧化酶的变化,探讨体内氧自由基损伤与KBD的关系.方法 在青海省兴海县KBD病区唐乃亥乡小学、下鹿圈村小学以及曲什安乡小学选择7～ 12岁的KBD患儿64名作为KBD组,46名健康儿童作为内对照组,在非病区陕西省长安区南樊小学选择59名健康儿童作为外对照组.分别采集其枕部发样和清晨空腹外周血,采用单纯随机抽样方法在每组中各抽取20例发样及血样,应用2,3-二告氨基萘(DAN)荧光法测定发硒和血硒水平;通过生化方法检测样本血清中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力,总抗氧化能力(T-AOC)及MDA水平.结果 KBD组发硒[(67.64±17.28)μg/L]、血硒[(36.27±13.29)μg/L]和GSH-Px活力[ (59.53±25.23)kU/L]明显低于内对照组[(153.32±24.31)、(63.06±13.66) μg/L,(91.88±22.99)kU/L]和外对照组[ (242.35±38.56)、(98.93±17.18)μg/L,( 122.68±41.74)kU/L],且内对照组明显低于外对照组(P均＜ 0.05);KBD组和内对照组SOD活力[(55.80±8.14)、(57.45±6.96)kU/L]、CAT活力[(16.45±5.61)、( 15.63±9.18)kU/L]和T-AOC水平[(19.80±6.64)、(21.71±8.82)kU/L]均高于外对照组[(42.79±8.10)、(6.05±2.71)、(13.56±5.38)kU/L,P均＜0.05];KBD组MDA水平[(4.64±1.11 )μmol/L]高于内对照组[(3.31±1.22) μmol/L]和外对照组[(3.43±1.29) μmol/L,P均＜0.05].结论 KBD存在氧化应激损伤,抗氧化酶在KBD病区儿童血清中升高是一种代偿性反应,体内氧自由基损伤与KBD的发生发展可能有一定的关系.     

阿托伐他汀对急性冠脉综合征患者血清高敏C反应蛋白和妊娠相关血浆蛋白-A水平的影响

目的探讨阿托伐他汀干预对急性冠脉综合征(ACS)患者血清高敏C反应蛋白(hs-CRP)和妊娠相关血浆蛋白-A(PAPP-A)水平的影响。方法采用酶联免疫吸附法测定不稳定型心绞痛患者(UAP,n=37)、急性心肌梗死患者(AMI,n=24)、稳定型心绞痛患者(SAP,n=29)和健康体检者(n=32)的hs-CRP和PAPP-A水平。同时将ACS患者(包括UAP和AMI组,n=61)随机分为常规治疗组(n=30)和阿托伐他汀干预组(阿托伐他汀10mg/d,n=31),并于治疗前后分别测定血清hs-CRP和PAPP-A水平。结果(1)hs-CRP和PAPP-A水平在UAP组[(16.7±1.24)mg/L,(63.88±1.82)μg/L]、AMI组[(18.52±1.96)mg/L,(66.41±1.24)μg/L]比SAP组[(4.6±1.16)mg/L,(47.56±0.72)μg/L]、正常对照组[(3.2±0.88)mg/L,(45.17±1.28)μg/L]显著升高(P<0.05)。(2)2周后,阿托伐他汀干预组血清hs-CRP和PAPP-A水平较治疗前明显降低[hs-CRP(18.52±2.37)mg/Lvs.(3.58±1.33)mg/L;PAPP-A(67.83±2.15)μg/Lvs.(45.62±1.58)μg/L,P<0.05],且较常规治疗组治疗2周后亦有显著降低[hs-CRP(3.58±1.33)mg/Lvs.(5.23±1.98)mg/L;PAPP-A(45.62±1.58)μg/Lvs.(51.35±2.15)μg/L,P<0.05]。结论阿托伐他汀干预可以减少急性冠脉综合征患者动脉粥样硬化斑块的炎症反应,具有稳定斑块的作用。     

Ecology,Asthma and Murray Peshkin

Serum total immunoglobulin E (IgE) was measured in 88 normal adults and 100 adults with asthma using Phadebas IgE PRIST kits. The geometric mean IgE in our normal subjects was found to be higher than that reported by some investigators but was similar to or lower than that reported by others without showing any geographic pattern. It increased progressively from 92 kU/L in normal controls through 205 kU/L in cases of asthma with low atopic scores to 464 kU/L in asthma cases with high atopic scores. There was wide variation in IgE levels among individuals, making it difficult to use it to classify any subject into one of these groups. However, as a group, female asthmatics had lower mean IgE levels (182 kU/L) than did men (577 kU/L), and a higher proportion of them had low atopic scores (60% of the women versus 47% of the men). This suggests that probably more women had intrinsic asthma, while extrinsic asthma was more common among men in the population studied.     

多发性骨髓瘤骨病患者骨代谢及调节因子水平及其意义

目的 研究多发性骨髓瘤(MM)患者血清Dickkopf-1(DKK-1)、可溶性核因子κB配体受体激活剂(sRANKL)、护骨素(OPG)和骨代谢因子[碱性磷酸酶(ALP)、抗酒石酸酸性磷酸酶(TRACP-5b)]水平及其与MM分期、溶骨性病变的关系,探讨骨代谢及调节因子DKK-1和sRANKL在MM骨病中的临床意义.方法 采用双抗体夹心ELISA检测30例初诊MM患者和20例健康人血清DKK-1、sRANKL、OPG、ALP、TRACP-5b等骨代谢因子及调节因子的水平.结果 MM患者血清DKK-1、sRANKL、OPG和TRACP水平均显著高于健康人,分别为42.96μg/L比5.33 μg/L,1.83pmol/L比0.79 pmol/L,1799.30 pmol/L比822.40 pmol/L,5.81 U/L比0.28 U/L,P值均＜0.05.国际分期系统(ISS)分期Ⅲ期患者血清DKK-1水平(46.33 μg/L)和sRANKL(2.26 pmol/L)水平显著高于Ⅰ/Ⅱ期患者(37.91μgL和1.19 pmol/L,P值均＜0.05).3处以上与1～3处溶骨病变患者相比,血清DKK-1、sRANKL和TRACP-5b水平显著升高(46.30μg/L比31.98μg/L,2.18 pmol/L比0.69 pmol/L,6.02 U/L比5.13 U/L,P值均＜0.05).结论 MM患者血清DKK-1、sRANKL、OPG和TRACP-5b水平均显著高于健康人;血清DKK-1、sRANKL水平与MM分期及溶骨病变程度相关.

Abstract：

Objective To detect serum concentrations of Dickkopf-1(DKK-1) and soluble receptor activator of nuclear factor-κB ligand (sRANKL) in patients with multiple myeloma (MM) and to investigate its clinical significance. Methods Serum DKK-1, sRANKL, osteoporotegerin(OPG) and tartrate-resistant acid phosphatase 5b (TRACP-5b) levels were quantified in 30 newly diagnosed MM patients and 20 healthy control subjects by using sandwich ELISA. Results The serum DKK-1, sRANKL,OPG and TRACP-5b levels were significantly higher than those in the healthy controls (42.96 μg/L vs 5.33 μg/L, 1.83 pmol/Lvs 0. 79 pmol/L, 1799. 30 pmol/L vs 822.40 pmol/L, 5.81 U/L vs 0. 28 U/L, respectively; all P＜0. 05). Serum levels of DKK-1 were positively correlated with sRANKL and TRACP-5b, respectively.Serum concentrations of DKK-1 and sRANKL were significantly elevated in stage Ⅲvs stages Ⅰ and Ⅱaccording to International Staging System (ISS) (46. 33 μg/L vs 37.91 μg/L, 2.26 pmol/L vs 1.19pmol/L, respectively, all P ＜0.05). Serum concentrations of DKK-1 , sRANKL and TRACP-5b were significantly higher in patients with more than 3 lytic bone lesions than those with only 1-3 lytic bone lesions (46. 30 μg/L vs 31.98 μg/L, 2. 18 pmol/L vs 0. 69 pmol/L, 6.02 U/L vs 5. 13 U/L, all P ＜ 0.05).Conclusions MM patients have increased serum DKK-1, sRANKL, OPG and TRACP-5b levels as compared with the healthy controls. Serum concentrations of DKK-1 and sRANKL have close relationship with MM stage and lytic bone disease.     

大鼠抑郁障碍与心血管组织炎性标记物的相关性研究

目的探讨强迫游泳抑郁模型大鼠抑郁障碍与炎性标记物的相关性。方法健康SD大鼠随机抽签分为对照组和模型组,应用21 d强迫游泳试验建立大鼠抑郁模型,模型组大鼠包括SS组(9只,应激+生理盐水腹腔注射)、SF组(8只,应激+氟西汀腹腔注射)和SI组(8只,应激+丙咪嗪腹腔注射)。对照组大鼠包括CS组(10只,生理盐水腹腔注射),CI组(7只,丙咪嗪腹腔注射)。应用透射免疫比浊法和酶联免疫法检测外周血炎性标记物高敏C反应蛋白(hsCRP)、单核细胞趋化因子1(MCP-1)水平,应用免疫组织化学染色法和蛋白印迹法检测血管组织核因子-κB(NF-κB)p65、抑制蛋白κB(IκB)-α、MCP-1表达水平。结果应激后模型组外周血hsCRP和MCP-1含量明显高于CS、CI组,SS组与CS组hsCRP水平分别为(0.26±0.07)mg/L和(0.15±0.06)mg/L(P<0.01),SS组与CS组MCP-1水平分别为(52.02±18.83)ng/L和(22.52±8.45)ng/L(P<0.01)。血管组织中MCP-1、NF-κB p65表达显著增强,SS组与CS组MCP-1水平分别为(1.78±0.36)mg/L和(0.85±0.15)mg/L,SS组与CS组NF-κB p65水平分别为(0.88±0.16)和(0.12±0.04)(P<0.01),胞浆中IκB-α表达显著减少,SS组与CS组IκB-α水平分别为(0.48±0.07)和(2.07±0.48)(P<0.01)。结论抑郁障碍通过NF-κB表达增强来启动炎症反应。