应用脱细胞异体真皮移植增粗阴茎的临床研究

目的：研究应用脱细胞异体真皮移植增粗阴茎的效果、手术并发症及移植物应植入的最佳解剖层次。方法：应用脱细胞异体真皮植入增粗阴茎25例,A组13例,移植物植入Buck’s筋膜深面,白膜浅面;B组12例,移植物植入Dartos筋膜深面,Buck’s筋膜浅面。结果：术后阴茎中段周径增大1．1～3．2cm,A、B两组均无阴茎畸形、勃起功能障碍、脱细胞真皮外露等并发症发生,A组4例早期出现龟头麻木,3个月后恢复正常,B组无类似病例。结论：应用脱细胞异体真皮移植增粗阴茎效果明显,手术创伤小,无供区损伤,但植入Buck’s筋膜深面可引起术后早期龟头麻木。     

阴茎延长同期行脱细胞异体真皮基质补片双平面植入阴茎增粗术

目的探讨阴茎延长同期行脱细胞异体真皮基质（acellular dermal matrix,ADM）补片双平面植入阴茎增粗术的方法与效果。方法采用阴茎根部倒V形切口。离断阴茎浅悬韧带后,沿阴茎纵轴切开Dartos筋膜,在其深面向远端分离。距冠状沟1．5～2．0cm处环形切开Buck筋膜,将补片前部植于Buck筋膜与白膜间,后部植于Dartos筋膜与Buck筋膜间。缝合Dartos筋膜切口,V—Y成形术闭合阴茎根部切口。结果35例术后无1例发生阴茎皮肤坏死、补片外露并发症。25例获随访6～24个月,对阴茎外形均感满意;无1例出现补片移位或皱褶、阴茎头感觉异常;其中21例已婚者均感性生活满意。结论经阴摹根部切口行脱细胞异体真皮基质补片双平面植入阴茎增粗术,通过调整补片植入层次,在确保补片足够的组织覆盖及刚茎皮肤血供情况下,在I期内行延长并增粗阴：拳术,具有并发症少、疗效满意的优点。     

应用脱细胞异体真皮植入Bucks筋膜下加大阴茎

目的探讨一种加大阴茎的手术方法。方法将脱细胞异体真皮填充在阴茎Bucks筋膜与白膜之间加大阴茎。结果自2002年3月以来,我们在临床应用12例,术后自然状态下阴茎周径加大13～31cm,平均26cm,术后3个月有正常的性生活。1例因包扎过紧至阴茎皮肤部分坏死,经转移阴囊皮瓣修复愈合。结论该方法用于阴茎加大,创伤小、操作简便、效果确实,无不良反应。     

异体脱细胞真皮基质的研究与应用

脱细胞真皮基质 (ａｃｅｌｌｕｌａｒｄｅｒｍａｌｍａｔｒｉｘ ,ＡＤＭ)是异体皮经特殊处理 ,去除其细胞成分后得到的一种真皮替代品。由Ｌｉｖｅｓｅｙ[1] 开发研制 ,Ｌｉｆｅｃｅｌｌ公司生产 ,商品名为ＡｌｌｏＤｅｒｍ ,已获美国ＦＤＡ批准应用于临床 ,近几年在烧伤和整形外科领域中得到了广泛的应用和发展。1　脱细胞真皮基质的制备方法ＡＤＭ的制备包含去表皮、脱真皮细胞及冷冻干燥处理 3个基本步骤 ,有两种方法。一是尸体皮经高渗盐水和 12烷基硫酸钠 (ＳＤＳ)处理 ,形成ＮａＣｌ ＳＤＳＡＤＭ[1,2 ] ;二是经ＤｉｓｐａｓｅⅡ处理…     

真皮脂肪复合组织条移植在阴茎增粗延长术中的应用

目的 探讨应用真皮脂肪复合组织条游离移植行阴茎增粗延长术的方法及临床疗效.方法 2004年5月至2010年12月,应用自体真皮脂肪组织条游离移植加阴茎根部切口阴茎延长术,对24例患者行阴茎增粗延长.采用阴茎根部切口,切断阴茎浅悬韧带及部分深悬韧带进行阴茎延长,同时在阴茎深筋膜与海绵体白膜间游离移植长6.0～9.5 cm,宽1.2～1.5 cm,厚度0.6～0.8 cm真皮脂肪复合组织条,自耻骨前区腔隙直至阴茎冠状沟,以增粗阴茎.结果 24例中23例一期愈合,1例发生切口脂肪液化,换药后愈合.阴茎常态及勃起后形态良好,勃起时延长长度为2.5 ～4.8 cm,平均3.2 cm;阴茎周径延长长度为1.8～3.0 cm,平均2.4 cm.本组18例获得3个月至5年的随访,所有患者对术后阴茎形态满意,对性生活质量满意度高,未见手术并发症.结论 真皮脂肪复合组织条移植加阴茎悬韧带切断术是一种较好的阴茎增粗延长术式,手术安全,效果肯定.     

脱细胞异体真皮在鼻整形术中的临床观察

目的观察脱细胞异体真皮在隆鼻术中的临床应用情况,寻找一种更为生理化的填充材料。方法在63例隆鼻术中,应用脱细胞异体真皮作为填充材料,并定期随防。结果 63例隆鼻术外形满意,术后吸收不明显,未发现排异情况,随访远期效果满意。结论脱细胞异体真皮在隆鼻术中部分替代固体硅橡胶,临床应用显示优越性,有推广应用价值。     

脱细胞异体真皮在烧伤后期瘢痕畸形整复中的应用

目的：评价脱细胞异体真皮在烧伤后期瘢痕畸形整复中的应用价值。方法：对32例烧伤后期瘢痕畸形病人整复后继发创面进行脱细胞异体真皮与自体刃厚皮片复合移植。结果：32例病人复合移植皮片全部成活，成活率为100％。成活的复合移植皮片具有外观平整，颜色与邻近正常皮肤相近似，无明显收缩，触之柔软，耐磨，弹性好等特点。结论：脱细胞异体真皮是烧伤后期瘢痕畸形整复中比较理想的材料。     

同种异体脱细胞真皮补片移植矫正白膜型阴茎弯曲的临床价值

目的 评价应用同种异体脱细胞真皮补片移植矫正白膜型阴茎弯曲的安全性与疗效.方法 2007年6月至2010年6月收治白膜型阴茎弯曲患者18例,年龄15～26岁,平均20岁.已婚12例.阴茎弯曲度30°～80°,平均55°;单侧弯曲17例,复杂弯曲1例.硬膜外麻醉或全麻,包皮环切切口入路,应用同种异体脱细胞真皮补片移植,延长曲侧海绵体白膜的术式治疗. 结果 18例阴茎弯曲均得到勃起直视下矫正,矫正后阴茎弯曲度0°～10°,平均4°.术后无感染、血肿、局部结节等并发症.18例随访3～24个月,无勃起功能障碍,未见弯曲复发、勃起硬结和形态畸形. 结论 同种异体脱细胞真皮补片矫正白膜型阴茎弯曲具有手术安全、疗效可靠、并发症少等优点.     

应用阴囊前动脉皮瓣修复同种异体脱细胞真皮基质补片阴茎增粗术后皮肤坏死

目的 探索阴茎延长同期经冠状沟近端包皮环形切口入路同种异体脱细胞真皮基质(ADM)补片阴茎增粗术后发生皮肤坏死的原因,并探讨应用一侧阴囊前动脉皮瓣修复缺损的疗效.方法 自2009年3月至2011年10月,共治疗8例阴茎延长同期经包皮入路ADM补片阴茎增粗术后皮肤坏死的患者,缺损面积7.4～10.0cm2,平均8.5cm2,均采用一侧阴囊前动脉带蒂皮瓣修复创面,供区直接缝合.结果 本组8例皮瓣均完全成活,无一例取出ADM补片,受区及供区切口均Ⅰ期愈合,阴茎外观满意.术后随访3～6个月,阴茎勃起均正常.结论 阴茎延长术同期经冠状沟近端包皮环形切口入路行ADM补片阴茎增粗术易发生阴茎背侧皮肤缺血坏死,不宜同期手术.以一侧阴囊前动脉为蒂的带蒂阴囊皮瓣具有血供可靠,转移方便,色泽、质地与阴茎皮肤接近的优点,修复阴茎皮肤缺损后形态满意并可保留ADM补片,可作为治疗此类并发症的首选术式.     

脱细胞异体真皮在烧伤后瘢痕修复中的应用

目的：探讨脱细胞异体真皮在烧伤后期瘢痕修复中的应用价值。方法：2006年2月-2007年2月入院治疗的21例烧伤后遗留瘢痕患者（A组）,采用切开并充分松解挛缩的瘢痕组织,利用脱细胞异体真皮加自体刃厚皮移植于继发创面的方法治疗。随机抽取同期21例采用瘢痕松解切除后进行中厚皮移植的患者（B组）及21例进行次全厚皮移植的患者（C组）作对照,比较3组患者的皮片成活情况以及术后1年的随访情况。结果：A组中皮片坏死率较其余两组略高;1年后随访发现,A组在受皮区皮片挛缩程度,外观平整度、色素改变及供皮区瘢痕形成方面均明显优于B组,与C组相当;C组仅适用于较小瘢痕的修复。结论：脱细胞异体真皮加自体皮复合移植是烧伤后期瘢痕修复的有效手段。     

异体脱细胞真皮在聚丙烯酰胺水凝胶取出术后乳房假体覆盖缺损修复中的应用

目的 乳房内聚丙烯酰胺水凝胶因并发症取出后,在进行假体置入时,由于局部组织的缺损会导致乳房假体覆盖不全,探讨采用异体脱细胞真皮修复聚丙烯酰胺水凝胶取出术乳房局部缺损的效果.方法 对46例患者进行乳房内聚丙烯水凝胶取出术后乳房形态的修复,在采用假体置入的同时,以异体脱细胞真皮修复乳房覆盖假体的局部缺损.置入乳房假体后,将脱细胞真皮在保持一定的张力时缝合固定于周围组织.结果 术后随访6个月至2年,患者乳房形态都获得了很好的修复,无假体疝出及包膜挛缩的发生.术后13个月病理检查显示,置入的脱细胞真皮与周围组织融合良好.结论 通过采用异体脱细胞真皮修复缺损的腺体或肌肉,虽然不能明显增加皮下组织的厚度,但是能够保持假体周围张力的连续性,避免因为局部张力的突然减少而产生假体膨出,是值得推荐的一种局部乳房缺损的修复方法.     

脱细胞异体真皮与自体瘢痕薄皮复合移植的临床应用

目的:为大面积深度烧伤后期继发瘢痕挛缩、功能障碍且皮源不足的患者寻求比较理想的治疗手段.方法:切开松解功能区孪缩的瘢痕组织,将脱细胞异体真皮与自体大张瘢痕薄皮复合移植覆盖创面,加压固定包扎.结果:5例患者12个创面中,除一个创面皮片部分坏死外,余均成活.术区平整、柔软,所植皮片挛缩轻,无瘢痕增生,功能恢复良好.结论:脱细胞异体真皮 自体瘢痕薄皮复合移植是目前修复大面积深度烧伤患者功能部位创面,实现功能重建的有效方法.     

The effect of skin expansion on acellular dermis in an experimental model

Objective: Although acellular dermis is widely used in prosthesis-based breast reconstruction, there is no study evaluating its expandability and histological, ultra-structural related changes. The purpose of this study is to determine whether acellular dermis could be expanded and whether the histological changes occurring in the skin with tissue expansion is different from normal when acellular dermis is used.

Methods: Twenty-two male Wistar rats were used in this study. In group 1 (n?=?6), a rectangular-shaped tissue expander with final volume of 35?ml was placed subcutaneously. In group 2 (n?=?6) tissue expander was placed submuscularly. In group 3 (n?=?6), a 4?×?5?cm, acellular dermis was inserted and sutured in place, tissue expander was placed underneath acellular dermis. Group 4 (n?=?4) was control, no procedure was done after creation of a pocket. After day 30, tissue expanders were inflated with 2?ml every 2 days with saline solution. On day 66, midvertical, midhorizontal surface expansion measurements were recorded. Samples were examined macroscopically. Biopsies were taken from mid-point of the expanded full-thickness flap. Histological evaluation, total skin thickness, epidermal thickness, and the amount of vascularisation were measured.

Results: There was no expansion noted on the acellular dermis; however, acellular dermis increased vascularity and decreased the thinning of the overlying skin and thickening of the epidermis when compared to other groups.

Conclusion: Acellular dermis use can be beneficial in implant-based breast reconstruction in terms of morbidity and its tissue support.     

Wound healing effect of acellular artificial dermis containing extracellular matrix secreted by human skin fibroblasts

In this study, an acellular artificial dermis, composed of human collagen and glycosaminoglycan (GAG) secreted by cultured human fibroblasts on a bovine collagen sponge, was developed. Much of the newly secreted extracellular matrix (ECM) remained after the cell removal process. The main theme of this study focused on the matrix, rather than the viable cell components of the skin, as the major dermal deficit in the wound. Both the acellular artificial and bioartificial dermises, containing viable cells with ECM, were significantly less soluble than the collagen sponge, and the relative GAG content in the bioartificial and acellular artificial dermises was approximately 115-120% of the chondroitin-6-sulfate (CS) content found in the collagen sponge. In the group receiving the collagen sponge, the wound area gradually decreased to approximately 10% of its original area, while in the groups receiving the bioartificial and acellular artificial dermises, the wound area also gradually decreased to approximately 60 and 50%, respectively, of the original size over the 5 weeks after grafting. Both the bioartificial and acellular artificial dermises formed thicker, denser collagen fibers; more new blood vessel formation was observed in both cases. The basement membrane of the regenerated epidermal-dermal junction was thicker and more linear in the acellular artificial dermis graft than in the collagen sponge graft. In conclusion, the wound healing effects of acellular artificial dermis are no less than those of the bioartificial dermis, and much better than the collagen sponge graft with respect to wound contraction, angiogenesis, collagen formation, and basement membrane repair.     

阴茎背神经切断术治疗早泄

目的评价阴茎背神经切断术治疗早泄的效果。方法自1997~2006年门诊早泄患者19例行阴茎背神经切断术治疗早泄。局麻下于阴茎背侧距冠状沟0.5~1cm处做2~3cm横切口,切开深筋膜,暴露左右两侧之阴茎背神经,并切除部分神经分支。记录患者手术前后阴道内射精潜伏时间和夫妻双方性交满意度。结果19例患者术前平均阴道内射精潜伏时间和性交满意度分别为(1.01±0.58)min(0.10~1.90min)和(14.89±6.08)%(5%~25%),术后平均射精潜伏期和性交满意度分别为(4.14±2.99)min(0.40~9.10min)和(57.47±28.28)%(10%~87%),手术前后相比P<0.01。19例中15例有效,有效率为78.95%,4例无效,2例出现术后轻度局部疼痛,1周后缓解。结论阴茎背神经切断术是一种治疗早泄的有效方法,适用于治疗年轻且不合并ED的患者。     

Application of micronised acellular dermal matrix for primary premature ejaculation: A preliminary study

This study aimed to explore the efficacy of injection of micronised acellular dermal matrix (MADM) particles for treating primary premature ejaculation. This study was a prospective single-arm clinical trial. Thirty patients who met the surgical indications were enrolled. MADM particles, mixed in platelet-rich plasma, were injected into Buck's fascia to spread over the dorsal penile nerve, suppress the influx of nerve impulses and, thus, reduce penis sensitivity. We evaluated the changes according to intravaginal ejaculation latency time using a stopwatch and a premature ejaculation diagnostic tool. Meanwhile, we also recorded sexual partner satisfaction and adverse events. All patients recovered well after surgery with no complications such as infections or allergies. The mean intravaginal ejaculation latency time before surgery was 0.72 ± 0.28 min, compared with 2.41 ± 0.54 min, 2.64 ± 0.41 min, 2.79 ± 0.25 min and 2.89 ± 0.35 min at, respectively, 4, 8, 16 and 20 weeks after surgery. The premature ejaculation diagnostic values and sexual partner satisfaction had significantly improved after treatment. Injection of MADM particles is an effective, safe and simple method for treating premature ejaculation.     

Safety and efficacy of tramadol hydrochloride on treatment of premature ejaculation

Premature ejaculation (PE) is the most common sexual disorder. It affects 20%–30% of adult men; the aetiology of this condition has not yet been elucidated. The aim of this study is to evaluate the efficacy, safety, tolerability, undesirable effects and improved satisfaction with sexual intercourse with tramadol hydrochloride at different dosages for the treatment of PE. A total of 300 patients who presented with lifelong (primary) PE were included in this study. The study was performed for 28 weeks, in which placebo (starch tablet) was given for 4 weeks, and active ingredient (tramadol hydrochloride) was administered at different therapeutic dosages for 24 weeks. Patients were divided into three equal groups, each consisting of 100 patients. The first group (A) was given tramadol hydrochloride capsule 25 mg. The second group (B) was given tramadol hydrochloride capsule 50 mg. The third group (C) was given tramadol hydrochloride capsule 100 mg. All of the 300 participants included completed the study voluntarily. The age of the patients varied from 25 to 50 years. After the treatment period, the recorded data were collected for each group and analysed. The results showed a highly significant increase in the mean intravaginal ejaculatory latency time (IELT) in all groups compared to baseline data (P<0.0001). We concluded that using tramadol hydrochloride at different doses on demand for the treatment of PE is effective, safe and tolerable, with minimal undesirable effects, and approval for this indication should be sought.     

Safety and efficacy of vardenafil versus sertraline in the treatment of premature ejaculation: a randomised, prospective and crossover study

We investigated safety and efficacy of vardenafil and sertraline in premature ejaculation (PE). Seventy-two men graded their primary PE on a scale of 0–8 (0 = almost never, 8 = almost always). Intravaginal ejaculatory latency time (IELT) was measured. Patients were included if they scored their PE as 4 or greater and their IELTs were less than 1.30 min. After 6 weeks of behavioural psychosexual therapy, 49 patients still had a PE of 4 or greater and an IELT less than 1.30 min and they were randomised: 6 weeks vardenafil (10 mg) or sertraline (50 mg). After a wash-out phase for 1 week, medication was changed in a cross-over design. Initially, all 72 men with PE received behavioural therapy. Twenty-three men were satisfied with treatment and excluded. The remaining 49 men graded their PE as 5.94 ± 1.6 and IELT was 0.59 min and patients were randomised. Four men discontinued the study. Vardenafil improved PE grading: 2.7 ± 2.1 ( P  < 0.01) and IELT increased to 5.01 ± 3.69 ( P  < 0.001). PE grading improved 1.92 ± 1.32, ( P  < 0.01) and IELT 3.12 ± 1.89 ( P  < 0.001) with sertraline. It is concluded that vardenafil and sertraline are useful agents in the pharmacological treatment of PE.     

盐酸舍曲林治疗早泄的临床研究

目的:观察盐酸舍曲林(左洛复)治疗早泄的疗效和副作用。方法:以盐酸氟西汀(百忧解)为阳性对照,分别以口服盐酸舍曲林50mg/d和盐酸氟西汀20mg/d治疗早泄患者,疗程4周,通过问卷调查两种药物的疗效及副作用。结果:66例患者完成研究,两种药物治疗早泄的有效率盐酸舍曲林为73.7%(28/38)、盐酸氟西汀为71.4%(20/28),差异无统计学意义(P>0.05);两种药物的副作用发生率也无明显差异。结论:抗抑郁药盐酸舍曲林与盐酸氟西汀一样,也有良好的治疗早泄作用,但具有更好的患者依丛性。