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目的:通过对口腔扁平苔藓(OLP)局部组织及外周血中IL-2、IL-10表达进行研究,进-步探讨OLP发病机制。方法:应用免疫组织化学SABC法检测30例OLP中IL-2、IL-10的表达数量及分布,同时用ELISA法检测外周血中IL-2、IL-10的含量。结果:①在病损组织中,OLP组中IL-2、IL-10表达显著高于对照组(P〈0.05)。IL-2主要分布在淋巴细胞浸润带,在基底膜附近较集中;IL-10主要分布在固有层,远离基底膜。②在外周血中,糜烂型OLP组IL-2、IL-10含量明显高于对照组和非糜烂型OLP组(P〈O.05)。结论:①根据IL-2、IL-10在OLP中表达和分布情况,提示IL-2、IL-10在OLP发病机制中参与了机体免疫的调节和介导作用。②外周血中IL-10高表达,提示糜烂型OLP患者全身的免疫反应参与了本病的形成或可能伴有全身免疫系统功能紊乱。 相似文献
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OLP患者唾液和血清中骨桥蛋白等细胞因子的表达 总被引:2,自引:1,他引:1
目的:研究口腔扁平苔藓(OLP)患者唾液和血清中骨桥蛋白(OPN)等细胞因子的含量及其相互关系。方法:经病理学检查确诊的OLP患者26例,其中充血糜烂型12型,网纹/斑块型14例。酶联免疫吸附试验(ELISA)检测患者唾液和血清中OPN,肿瘤坏死因子-α(TNF-α),转化生长因子-β1(TGF-β1)的含量。以26名健康志愿者作为正常对照。结果:OLP患者唾液和血清OPN,TNF-α含量与正常对照组相比均有升高(P〈0.05);充血糜烂型OLP的TGF-β1唾液和血清水平显著高于网纹/斑块型OLP(P〈0.05)。三种抗体在唾液和血清中表现出明显的相关关系。结论:OPN在OLP患者唾液和血清中含量升高,唾液和血清中OPN含量具有相关性。唾液作为无创伤性检测标本有望有限地代替血清标本应用于OLP与口腔白斑的临床检测。 相似文献
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目的 探讨不同类型口腔扁平苔藓(OLP)患者血清IL-2、IL-4、IL-6在OLP发病及治疗中的意义。方法 采用ELISA方法检测60例各型OLP患者血清IL-2、IL-4、IL-6的水平,记录患者的疼痛程度、充血和糜烂面积。结果 OLP患者血清IL-2、IL-4水平较对照组无显著差异,而各型OLP患者IL-6显著升高。糜烂型OLP患者IL-4水平显著高于对照。血清IL-2、IL-4水平与疼痛程度、充血面积和糜烂面积之间无相关性,而IL-6与三者呈正相关。结论 本研究支持自身免疫机制是OLP的发病机制之一的观点;在OLP的不同阶段患者的免疫状态是变化的;IL-6在OLP的发病中发挥了一定作用。血清IL-6水平也许可以作为动态监测OLP疾病进展的指标和疗效观察的指标。 相似文献
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目的观察口腔扁平苔藓(OLP)患者血清中Th型细胞因子IFN-γ、IL-10和IL-17表达,初步探讨其在OLP发生发展中的作用。方法选择2011—2014年在上海市口腔病防治院口腔内科就诊的OLP患者15例,健康志愿者15例,采集静脉血,酶联免疫吸附试验(ELISA)检测血清中IFN-γ、IL-10和IL-17含量。结果实验组、对照组OLP患者血清中Th1型细胞因子IFN-γ的含量无显著差异(P>0.05),Th2型细胞因子IL-10含量为(43.3±4.6)、(29.0±5.1)pg/m L,实验组高于对照组(P<0.05)。IFN-γ与IL-10的比值(即Th1/Th2)为0.418、0.712,实验组较对照组的比值减小。两组Th17型细胞因子IL-17含量为(23.6±2.7)、(16.8±3.3)pg/m L,实验组高于正常对照组(P<0.05)。结论 Th型相关细胞因子失衡与OLP的发生发展密切相关。 相似文献
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本文通过对43例口腔扁平苔藓(OLP)患者混合全唾液 pH 值、流率、乳酸脱氢酶(LDH)及其同工酶谱的测定分析,发现 OLP 的发病与其外环境——唾液的变化有十分密切的关系。LDH及其同工酶是组织破坏的敏感指标,本研究结果显示,OLP 患者唾液 LDH 总活性高于对照组(P<0.05),其中糜烂型较非糜烂型高。患者唾液同工酶中,LDH_1、LDH_2、LDH_3降低,LDH_4、LDH_5升高(P<0.01),说明 LDH 的变化与 OLP 病情的轻重有关,是一有用的辅助判断病情及预后的客观生化指标。 相似文献
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目的:探讨OLP患者唾液流量和pH值的变化与OLP患者感染白色念珠菌的相关性,并对其作为感染白色念珠菌的风险评估。方法:研究对象分为OLP糜烂组、OLP非糜烂组和正常对照组,每组20人。收集无刺激唾液,检测唾液流量与唾液pH,通过科玛嘉显色培养基显色培养唾液,对结果进行统计分析。结果:OLP糜烂组与正常对照组的唾液样本中白色念珠菌的表达差异有统计学意义,OLP糜烂组唾液流量对OLP非糜烂组和健康对照组的差异有统计学意义。结论:OLP患者唾液流量与白色念珠菌感染成负相关。OLP患者唾液流量可以作为评估OLP患者感染白色念珠菌风险的指标。 相似文献
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口腔扁平苔藓患者唾液中亚硝酸根的测定及其意义 总被引:1,自引:0,他引:1
目的 :探讨口腔扁平苔藓 (OLP)患者唾液中一氧化氮 (NO)水平的变化。方法 :采用示波极谱法测定了 6 0例OLP患者 (糜烂型 30例、光滑型 30例 )唾液中NO2 -浓度 ,按性别及年龄 2∶1配对 ,同期收集 30例健康志愿者唾液为对照组。并以浓度Χ唾液流量计算唾液NO2 -产出。结果 :所得数据表明 :糜烂型OLP组唾液NO2 -浓度 2 2 .6 9± 15 .0 3μmol/L ,显著高于健康对照组 (10 .74± 7.98μmol/L) (P <0 .0 5 )与光滑型OLP组 (11.5 5± 6 .5 2 μmol/L) ,P <0 .0 0 2。结论 :本研究中发现OLP患者唾液NO2 -水平上调 ,这种差异主要在于糜烂型OLP患者。推论NO可能在OLP发病及发展中起一定作用。本研究为进一步研究NO在OLP发病及发展中的作用奠定了初步基础 相似文献
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口腔扁平苔藓患者唾液bFGF检测及意义 总被引:1,自引:0,他引:1
碱性成纤维细胞生长因子 (bFGF)是成纤维细胞生长因子家族的重要成员之一 ,由 14 6个氨基酸组成 ,存在于神经组织、脑垂体、肾上腺皮质、胎盘以及唾液和泪液中 ,对造血和伤口组织愈合非常重要。我们检测了口腔扁平苔藓 (OLP)患者唾液中bFGF的浓度 ,并分析其在OLP发病中的作用 , 相似文献
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目的:进一步探索IL-17在OLP局部病变中的表达及其作用机制.方法:16位OLP患者和5位健康志愿者被募集参与本试验.采用免疫荧光染色法检测IL-17 (interleukin-17)在OLP病损组织中的表达.进一步采用免疫组织化学染色法检测IL-17受体(interleukin-17 receptor,IL-17R)在OLP病损组织中的表达情况.结果:IL-17+细胞散在分布于OLP病损组织黏膜固有层中.OLP局部组织中均存在IL-17R的表达,IL-17R主要表达于上皮基底角质形成细胞;同时在上皮下淋巴细胞浸润层中也可见相当数量的炎症细胞表达IL-17R.结论:在OLP病损局部IL-17可与表达于上皮基底角质形成细胞和上皮下淋巴细胞浸润层中部分炎症细胞表面的特异性受体IL-17R结合,进而参与OLP的局部炎症反应. 相似文献
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目的 探讨KLF 6在口腔扁平苔藓发病机制及癌变中的作用。方法 分别采用免疫组化和Western blot方法对10例正常口腔黏膜、30例扁平苔藓患者和22例口腔鳞癌患者的上皮组织中KLF 6蛋白进行t检测,比较其在三者之间的差异。SPSS 14.0对数据进行检验。结果 扁平苔藓患者组织中KLF 6蛋白的表达明显低于正常口腔黏膜,两组之间具有显著性差异;口腔鳞癌患者组织中KLF 6蛋白的表达明显低于扁平苔藓组织,两组之间具有显著性差异。结论 KLF 6的表达异常可能在口腔扁平苔藓的发生发展及癌变的过程中起作用。 相似文献
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目的 :分析Ets 1(E2 6transformation specific)在口腔扁平苔藓 (OLP)的表达及意义。 方法 :采用免疫组化ABC法检测Ets 1蛋白在 2 0例口腔扁平苔藓和 8例正常口腔黏膜组织中的表达。结果 :70 % (14 /2 0 )的口腔扁平苔藓病例中Ets 1呈阳性表达 ,明显高于正常黏膜组织 ,两者比较有显著性差异 (P <0 .0 5 )。阳性表达率在溃疡型OLP与斑块型OLP间亦有显著性差异 (P <0 .0 5 ) ,并与病程相关 (P <0 .0 5 )。结论 :Ets 1在口腔扁平苔藓中过表达并与其发病有关。 相似文献
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OBJECTIVE: The expression pattern of lymphocyte function-associated antigen 3 (LFA-3) in the buccal mucosa of oral lichen planus (OLP) patients was compared to that of healthy controls to investigate the possible role of LFA-3 in cell interactions within OLP lesions.
MATERIALS AND METHODS: Samples of buccal mucosa from 17 clinically healthy individuals and 17 OLP lesions were analysed. Expression of LFA-3, CD2, CD3 and CD 14 was visualized by an immunoperoxidase technique and assessed microscopically.
RESULTS: In healthy buccal mucosa LFA-3 was expressed on keratinocytes, Langerhans cells within the epithelium and on endothelial cells in the lamina propria. In OLP patients a similar pattern of LFA-3 staining was observed. In addition, cytoplasmic LFA-3, without accompanying surface staining, was seen on a subpopul-ation of macrophage-like cells. Substantial amounts of LFA-3 also appeared to be associated with non-cellular components of the extracellular matrix within the inflammatory infiltrate.
CONCLUSIONS: We have obtained evidence for a previously undescribed localization of LFA-3 within macro-phages, and have observed that expression of LFA-3 is apparently elevated within OLP lesions. LFA-3 may play an important role in the pathogenesis of OLP. 相似文献
MATERIALS AND METHODS: Samples of buccal mucosa from 17 clinically healthy individuals and 17 OLP lesions were analysed. Expression of LFA-3, CD2, CD3 and CD 14 was visualized by an immunoperoxidase technique and assessed microscopically.
RESULTS: In healthy buccal mucosa LFA-3 was expressed on keratinocytes, Langerhans cells within the epithelium and on endothelial cells in the lamina propria. In OLP patients a similar pattern of LFA-3 staining was observed. In addition, cytoplasmic LFA-3, without accompanying surface staining, was seen on a subpopul-ation of macrophage-like cells. Substantial amounts of LFA-3 also appeared to be associated with non-cellular components of the extracellular matrix within the inflammatory infiltrate.
CONCLUSIONS: We have obtained evidence for a previously undescribed localization of LFA-3 within macro-phages, and have observed that expression of LFA-3 is apparently elevated within OLP lesions. LFA-3 may play an important role in the pathogenesis of OLP. 相似文献
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目的:研究外周血CD4+CD25+Foxp3+调节性T细胞(Treg细胞)和白介素15 (IL-15)的表达在口腔扁平苔藓(OLP)发病机制中的作用.方法:采用免疫荧光染色技术,经流式细胞仪检测36例口腔扁平苔藓患者和20例正常人外周血Treg表达水平.应用ELISA技术检测外周血IL-15的表达水平,采用SPSS16.0软件包对其表达差异进行统计学分析.结果:口腔扁平苔藓患者外周血CD4+CD25+Foxp3+ Treg细胞数量较正常组显著上升,IL-15表达水平也显著高于正常组,但是口腔扁平苔藓糜烂型与非糜烂型之间的差异无显著性.Treg的表达与IL-15的水平呈正相关(P<0.05).结论:Treg和IL-15的表达上升可能是OLP的发病机制之一. 相似文献
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Jing-Ling Xue Ming-Wen Fan Shuo-Zhi Wang Xin-Ming Chen Yuan Li Li Wang 《Journal of oral pathology & medicine》2005,34(8):467-472
BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical presentations that can be classified as reticular, atrophic or erosive. Although OLP is a relatively common disorder, the reports comprising large numbers of OLP patients with specific character are lacking in the literature. The purpose of this paper was to describe the clinical characteristics of OLP in 674 Chinese patients. METHODS: A total of 674 charts of patients with histologically confirmed OLP were collected from Stomatological Hospital of Wuhan University between 1963 and 2003. RESULTS: Of the 674 patients, 65.9% were women and 34.1% were men. The most common clinical presentation was the reticular type (51.3%), and symptomatic OLP was noted in 67.5% of the patients, mainly in those with the erosive form. The erosive presentations showed significantly longer duration, more sites affected and a much greater old patients predominance than reticular or atrophic ones. About 90.9% of the patients had multiple oral sites of involvement and isolated lower lip lichen planus were observed in 60 cases (8.9%) and isolated gingiva lichen in only one case (0.2%). Skin involvement of lichen planus was found in 11.4% of patients. No statistically significant differences could be identified between OLP and diabetes, cardiovascular disease, smoking or alcohol use. Precipitating factors that resulted in an exacerbation of the disease were frequently noted and included foods, stress, dental cusp and poor oral hygiene. The transformation of OLP into malignancy was observed in four patients at sites previously diagnosed by clinical examination as erosive or atrophic lichen planus. CONCLUSIONS: Patients with OLP in China usually present with distinctive clinical morphology and characteristic distribution and few may display lesions with a confusing array of forms mimicking other diseases. A long time follow up is of utmost importance to detect its malignant transformation. 相似文献