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我们采用福尔马林用药量800ml/m3[1]的方法观察福尔马林自然蒸发法对金黄色葡萄球菌、绿脓杆菌、大肠杆菌这三种细菌的灭活效果。结果表明,此法对这三种细菌均有灭活作用,效果确实,只要消毒液不低于800ml/m3即可。我们认为此种方法可以作为手术室常用的消毒方法。 相似文献
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福尔马林自然蒸发消毒法对细菌灭活作用的实验探讨 总被引:2,自引:0,他引:2
我们采用福尔马林用药量800ml/m^3的方法观察福尔马林自然蒸发法对金黄色葡萄球菌,绿脓杆菌,大肠杆菌这三种细菌的灭活效果。结果表明,此法对这三种细菌的有灭活作用,效果确定,只要消毒液不低于800ml/m63即可。我们认为此种方法可以作为手术室常用的消毒方法。 相似文献
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目的:观察几种常用消毒剂对HBsAg灭活效果.方法:以小手术刀片为载体置于HBsAg阳性血清中,污染后分别浸泡于几种常用消毒液中,经10分钟、20分钟、30分钟时分别取出,然后用酶联免疫吸附法分别检测HBsAg灭活情况,再将以上消毒液保存至第三天,第七天用同法各检测一次使用中的消毒液对HBsAg的灭活情况.结果:2%LJ强化戊二醛、0.2%过氧乙酸、0.5碘伏、5%“84“消毒液等经30分钟以上均能灭活HBsAg,但使用一周时的上述消毒液均失去灭活HBsAg作用,0.5%“84“、3%过氧乙酸对HBsAg完全不能灭活.结论:手术器械的浸泡消毒以戊二醛效果最好,且宜一周内更换一次消毒液. 相似文献
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过氧乙酸用于空气层流洁净病房的消毒研究 总被引:1,自引:0,他引:1
为观察过氧乙酸用于空气层流洁净病房空气消毒的效果,用1g/m^3、2g/m^3的过氧乙酸对空气层流洁净病房进行空气消毒。结果:2g/m^3过氧乙酸自然挥发熏蒸法进行空气层流洁净病房的消毒效果符合要求。提示2g/m^3过氧乙酸熏蒸法可用于空气层流洁净病房的空气消毒。 相似文献
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乙肝表面抗原HBsAg在我国人群中平均携带率为10%,防止乙肝病毒感染仍是医院感染中的重要问题。消毒液对医疗器械污染HBsAg灭活作用的研究曾有报道[1,2]。然而,消毒液对检测结果的实验干扰至今探讨甚少。为此,我们选择1:800三效热原灭活剂、1:20碘伏、0.2%过氧乙酸、2%戊二醛四种消毒液,实验观测了ELISA法检测HBsAg时的干扰现象,现将结果报道如下。1材料与方法1.1材料三效热原减活剂、碘伏、过氧乙酸、戊二醛、ELI-SA试剂、玻璃球载体、水浴箱、酶标仪等。1.2方法分别取玻璃球10个(直径0.8cm)浸入病人混合血清30ml(… 相似文献
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摘要 目的 研究一种新型过氧乙酸消毒灭菌粉对软式内镜的现场消毒效果。方法 软式内镜严格按照操作规范规定的流程清洗后,用过氧乙酸消毒灭菌粉和酸性氧化电位水进行消毒灭菌,并采用细菌培养和浓度检测方法对消毒的实际效果进行监测和评价。结果 两种不同消毒剂清洗内镜消毒效果的合格率为100%。过氧乙酸消毒灭菌粉连续使用8 h有效浓度维持在≥2 300 mg/L。结论 这种新型的过氧乙酸消毒灭菌粉应用于软式内镜消毒,提高了工作效率,节省水资源,取得快速有效的消毒效果,具有良好的安全性和稳定性。 相似文献
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目的比较不同消毒方法对采血车内空气消毒效果。方法通过自然沉降法采样和细菌检测方法,对空气消毒机、紫外线灯和过氧乙酸熏蒸法等不同方法消毒采血车内空气的效果进行了检测。结果臭氧消毒机、紫外线灯和过氧乙酸熏蒸等3种空气消毒方法按照各自使用方法处理1 h(过氧乙酸熏蒸2 h)之后,采血车内空气中细菌总数均存在不同程度超标。动态空气消毒机运行1 h后,采血车内空气质量均符合Ⅲ环境要求,且在工作期间保持运行状态,仍可保持空气质量始终符合要求。结论所试验的4种空气消毒方法,只有动态空气消毒机可用于采血车空气消毒。 相似文献
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检测了过氧乙酸对人巨细胞病毒污染表面的消毒效果。0.05%过氧乙酸作用2分钟,或0.10%过氧乙酸作用1分钟,可将试验表面的人巨细胞病毒全部灭活。 相似文献
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戊二醛复方消毒剂消毒效果的试验观察 总被引:11,自引:0,他引:11
对含有2%戊二醛与表面活性剂的复方消毒剂进行了杀菌效果检测。按8ml/m3用量喷雾其1∶10稀释液,作用30分钟,可使空气中金黄色葡萄球菌、肺炎链球菌等试验菌繁殖体全部消亡;浸泡物品5与10分钟,可分别破坏与杀灭物品表面的HBsAg与细菌繁殖体 相似文献
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Yong-Hui Tan Kai Li Xin-Yi Chen Ye Cao Alan R. Light Kai-Yuan Fu 《The journal of pain》2012,13(10):1008-1015
Protein tyrosine phosphorylation has been implicated in normal and pathological functions such as cell proliferation, migration and differentiation. Recently, some studies have shown that Src family kinases (SFKs) were involved in neurological disorders and neuropathic pain states in which microglial activation plays a role. In the formalin test, we have reported that microglia undergo at least 2 distinct stages of activation on the basis of signaling events regarding p38 mitogen-activated protein kinases (MAPK). Here, we investigated the involvement of SFKs signaling in a formalin pain animal model and the association with p38 MAPK. Our results showed that SFKs were activated in the spinal microglia beginning 1 day after peripheral formalin injection lasting for 7 days. Pretreatment with SFK specific inhibitor PP2 could not inhibit formalin-induced spontaneous pain behaviors. However, PP2 inhibited formalin injury, induced persistent mechanical hyperalgesia, and reversed microglial phospho-p38 expression as well using immunohistostaining and Western blot at day 3 and 7 after injection. Our results suggested that the activation of the Src/p38MAPK signaling cascade in spinal microglia contributed to late stage persistent mechanical hyperalgesia evoked by formalin injection into the paw. 相似文献
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Peripheral interactions between dextromethorphan,ketamine and amitriptyline on formalin-evoked behaviors and paw edema in rats 总被引:3,自引:0,他引:3
The local, peripheral administration of antidepressants and excitatory amino acid receptor antagonists can cause analgesia in a number of conditions. The present study examined the effects of combinations of dextromethorphan and ketamine, two clinically used N-methyl-D-aspartate (NMDA) receptor antagonists, with amitriptyline on formalin-evoked behaviors and paw edema. Pretreatment with amitriptyline or dextromethorphan (10-300 nmol) resulted in suppression of flinching behaviors induced by 2.5% formalin, but ketamine had no intrinsic effect. Combination of an inactive dose of dextromethorphan with amitriptyline, and vice versa, resulted in an increase of analgesia so that previously inactive doses now caused significant analgesia. Combinations of multiple doses of ketamine with amitriptyline did not modify the response to amitriptyline. Both dextromethorphan and ketamine increased the paw edema induced by formalin, and this was blocked by low doses of amitriptyline. In the absence of formalin, amitriptyline (1-100 nmol) caused a dose-related suppression of the paw edema produced by dextromethorphan and ketamine. Amitriptyline also blocked paw edema produced by 5-hydroxytryptamine and compound 48/80. Each of the drugs used in this study exerts multiple pharmacological effects. Increased analgesia by drug combinations (amitriptyline/dextromethorphan) could show the involvement of a number of these mechanisms (e.g. NMDA receptor blockade, blockage of sodium channels, blockage of biogenic amine receptors), while a lack of intensification (amitriptyline/ketamine) could reflect occluded actions due to expression of similar actions by the other drug. Paw edema induced by dextromethorphan and ketamine involves inhibition of biogenic amine reuptake, and the ability of amitriptyline to block biogenic amine receptors likely accounts for its inhibiton of these actions. Combinations of these particular agents could represent a method for augmented analgesia and minimization of local adverse reactions. 相似文献
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乙型肝炎血清学标志物抗力的比较 总被引:4,自引:0,他引:4
经检测,对钴-60辐照,病人血清中HBsAg、pHSA-R与HBV-DNA抗力较其中HBV-DNAp、HBeAg及纯化HBsAg者为强.HBV颗粒经17.90kGy剂量辐照后在电镜下仅见碎片.对所试含氯消毒剂、碘酊、高锰酸钾、盐酸与过氧化物消毒剂,病人血清中的HBsAg较其中pHSA-R、HBeAg及纯化HBsAg的抗力为强. 相似文献
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有机物对化学消毒效果的影响 总被引:1,自引:0,他引:1
试验测定血清等有机物对化学消毒剂的消耗率,发现血清对消毒剂有效成分消耗严重,碱性条件更使消耗量有所增加。磷酸钠可增强卤素消毒剂在有机物干扰下的杀菌作用。增加消毒剂用量,在有机物存在下亦可提高消毒效果。测定消毒剂有效成分余留浓度有可能作为判断消毒效果的间接指标。 相似文献
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<正> 近年来国内外已对小肠结肠炎耶尔森氏菌(下简称耶氏菌)进行了较为广泛的研究,但对受耶氏菌污染食品处理的研究甚少。我们选用过氧乙酸和漂白粉为消毒剂,测定其对肉类表面耶氏菌的消毒效果。 相似文献
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液相杂交技术在聚合酶链反应酶联免疫检测的应用 总被引:16,自引:1,他引:15
目的采用液相杂交技术,简化核酸杂交过程,建立乙肝病毒(HBV)的液相杂交的聚合酶链反应-酶联免疫检测(PCR-ELISA)方法。方法将5′端标记生物素的PCR扩增产物与5′端标记地高辛的探针呈液相混合,90℃2min,55℃1min杂交。杂交后产物被链霉亲和素酶标板固定,经酶标记抗地高辛标记抗体结合、显色。结果液相杂交酶联免疫检测条件优化探针浓度3pmol/次,酶标记抗体稀释度1∶1000。液相杂交时间3min,固相杂交时间120min,但其检测结果无明显差别。检测灵敏度1×10 相似文献