高度富集黄酮类成分的贯叶连翘提取物抗抑郁作用

目的探讨贯叶连翘提取物中高度富集的黄酮类成分的抗抑郁作用。方法采用小鼠强迫游泳实验、小鼠悬尾实验、开野实验和拮抗利血平所致的体温降低实验,分别以小鼠不动时间、自主活动数和体温下降值作为评价指标。结果在强迫游泳和悬尾实验中,40,80,160,240mg/kg剂量组贯叶连翘提取物均能显著缩短小鼠不动时间,20mg/kg剂量组无显著性差异;开野实验结果表明给药后小鼠的自主活动不同程度地减少;在利血平拮抗实验中,30,60,120mg/kg剂量组在3h,4h和5h各时间点体温下降值与模型对照组相比有显著性差异,240和480mg/kg两剂量组在各测定时间点体温下降值与模型对照组相比均有显著性差异。结论高度富集总黄酮的贯叶连翘提取物(弃除贯叶金丝桃素)有抗抑郁作用。     

越鞠胶囊与贯叶连翘抗抑郁作用的对比研究

目的:研究越鞠胶囊、贯叶连翘的抗抑郁作用。方法:采用小鼠悬尾实验、小鼠强迫游泳实验、利血平诱导小鼠体温下降实验和开场实验研究越鞠胶囊、贯叶连翘的抗抑郁作用。结果:在小鼠悬尾实验中,越鞠胶囊大剂量组、贯叶连翘大、中剂量组能明显缩短模型小鼠的不动时间;在小鼠强迫游泳实验中,越鞠胶囊、贯叶连翘各剂量组能明显缩短模型小鼠的不动时间;在利血平诱导的体温下降实验中,越鞠胶囊大剂量组、贯叶连翘大、中剂量组能明显抑制小鼠体温的下降;实验表明贯叶连翘提取物的作用效价强度要大于越鞠胶囊。结论:越鞠胶囊、贯叶连翘有一定的的抗抑郁作用,贯叶连翘提取物的作用效价强度要大于越鞠胶囊。     

白芍提取物治疗抑郁症的实验研究

目的:观察白芍提取物的抗抑郁作用。方法:采用小鼠悬尾、小鼠强迫游泳及利血平拮抗实验。白芍提取物22.5,45,90mg·kg-1ig7d后观察小鼠悬尾不动时间,强迫游泳不动时间及体温变化。结果:白芍提取物45,90mg·kg-1·d-1均可显著缩短小鼠悬尾及强迫游泳不动时间(P0.05),并显著对抗利血平所致的小鼠体温下降(P0.05)。结论:白芍提取物对小鼠具有明显的抗抑郁作用。     

大黄素甲醚抗抑郁作用的研究

目的:观察大黄素甲醚对小鼠抑郁模型的影响。方法:采用小鼠悬尾实验,小鼠强迫游泳实验,利血平拮抗实验。结果:大黄素甲醚可显著缩短小鼠悬尾及强迫游泳不动时间,且均可显著对抗利血平所致的小鼠体温下降、眼睑下垂及运动不能,与模型组相比有差异性。结论:大黄素甲醚100mg/kg具有明显的抗抑郁作用。     

贯郁胶囊治疗抑郁症的实验研究

目的:观察贯郁胶囊的抗抑郁作用。方法:小鼠悬尾、小鼠强迫游泳及利血平拮抗实验。结果:贯郁胶囊1. 2g kg、0. 6g kg均可显著缩短小鼠悬尾及强迫游泳不动时间(P <0 . 0 1,P <0. 0 5 ) ,并且均可显著对抗利血平所致的小鼠体温下降、眼睑下垂及运动不能(P <0. 0 1,P <0. 0 5 )。结论:贯郁胶囊具有明显的抗抑郁作用。     

越菊保和丸的抗抑郁作用研究

目的：研究越菊保和丸抗抑郁作用。方法：选取小鼠强迫游泳实验、小鼠悬尾应激实验、拮抗利血平所致的抑郁症状等实验指标研究越菊保和丸的抗抑郁作用。结果：越菊保和丸使小鼠悬尾不动时间和小鼠强迫游泳不动时间显著缩短（P〈0．05），并有显著拮抗利血平所致的体温下降作用和小鼠眼睑下垂及运动不能的作用（P〈0．05）。结论：越菊保和丸可能有抗抑郁的作用。     

柴胡加龙骨牡蛎汤抗抑郁作用研究

目的：研究柴胡加龙骨牡蛎汤抗抑郁作用。方法：采用小鼠强迫游泳、悬尾、高剂量阿朴吗啡拮抗、利血平拮抗、5－HTP诱导甩头等抑郁动物模型，观察柴胡加龙骨牡蛎汤的抗抑郁作用。结果：柴胡加龙骨牡蛎汤50、200、500mg/kg能缩短小鼠强迫游泳，悬尾不动时间，能拮抗高剂量阿朴吗啡，利血平降低小鼠体温作用，增加5－HTP诱导的甩头次数，结论：柴胡加龙骨牡蛎汤具有显的抗抑郁作用。     

解郁方抗抑郁作用的实验研究

目的:研究解郁方对小鼠抑郁模型的抗抑郁作用。方法:采用小鼠强迫游泳实验、小鼠悬尾实验和拮抗利血平所致的抑郁症状实验,研究解郁方的抗抑郁作用。结果:解郁方中、高剂量组均可显著缩短小鼠强迫游泳、悬尾不动时间(P<0.01),解郁方高剂量组拮抗利血平所致小鼠运动不能作用(P<0.01)、小鼠眼睑下垂作用(P<0.05),解郁方低、中、高剂量组拮抗利血平所致小鼠体温下降作用(P<0.01)。结论:解郁方有抗抑郁的作用。     

三种贯叶连翘组药用植物醇提物对小鼠的抗抑郁作用

目的：对贯叶连翘组药用植物的抗抑郁作用进行研究，为筛选新的药用品种以及对其品质评价提供药效学依据。方法：采用小鼠尾悬挂试验，小鼠强迫游泳试验抑郁模型对三种贯叶连翘组药用植物醇提物的抗抑郁作用进行研究。结果：在小鼠尾悬挂试验，小鼠强迫游泳试验中，元宝草和扬子小连翘醇提物物与贯叶连翘一样，可以对抗小鼠的失望行为，使实验动物的绝望时间（不动时间）缩短，具有抗抑郁作用，但是贯叶连翘醇提物的抗抑郁作用明显强于扬子小连翘。结论：三种川产贯叶连翘组药用植物醇提物在行为绝望动物模型上有明显的抗抑郁作用。     

淫羊藿提取物抗抑郁作用研究

目的研究淫羊藿提取物的抗抑郁作用。方法采用行为绝望模型悬尾试验(TST)和强迫游泳试验(FST)研究淫羊藿提取物对小鼠行为、脑内单胺氧化酶A(M AO-A)、单胺氧化酶B(M AO-B)活性与肝脏中M AO-A和M AO-B活性及丙二醛(M DA)水平的影响;采用利血平拮抗模型探讨淫羊藿提取物可能存在的抗抑郁作用途径。结果淫羊藿提取物(25、50、100、200 m g/kg)能显著缩短TST和FST小鼠悬尾和游泳不动时间,显著抑制TST小鼠脑和肝组织M AO-A和M AO-B活性,逆转肝组织M DA水平的升高。淫羊藿提取物对利血平所致小鼠体温的下降无明显改善作用。结论淫羊藿提取物具有一定抗抑郁作用。     

Effect of Hypericum perforatum on marble-burying by mice

The effect of Hypericum perforatum extract (LI 160) at a dose that exerts an antidepressive-like effect was studied in mice in the marble-burying test. Acute Hypericum perforatum (150, 300 and 500 mg/kg, p.o.) reduced immobility time in the forced swimming test. The number of marbles buried, but not locomotor activity, was reduced by acute treatment with Hypericum perforatum (150 and 300 mg/kg, p.o.). However, this effect was not seen after chronic treatment (21 days) with Hypericum perforatum (300 mg/kg, p.o.). Thus, Hypericum perforatum extract, at antidepressant dose, exerts an acute anxiolytic drug effect on the marble-burying test, which could indicate a potential anti-obsessive effect, although the development of tolerance could be an important drawback.     

柴胡提取组分抗抑郁作用的研究

目的 观察柴胡提取组分( CHB)的抗抑郁作用.方法 采用小鼠强迫游泳、悬尾两种“行为绝望”抑郁动物模型和利血平拮抗模型,观察CHB对小鼠游泳不动时间、悬尾不动时间以及利血平诱导的体温降低、眼睑下垂、运动不能的影响.结果 CHB 100,200 mg/kg均能不同程度地缩短小鼠强迫游泳和悬尾不动时间,能拮抗高剂量利血平致小鼠体温下降,改善利血平诱导的小鼠眼睑下垂,并呈现一定的量效关系.结论 CHB具有一定的抗抑郁作用.     

Effect of Hypericum perforatum L. Extract on Insulin Resistance and Lipid Metabolic Disorder in High‐Fat‐Diet Induced Obese Mice

Natural product Hypericum perforatum L. has been used in folk medicine to improve mental performance. However, the effect of H. perforatum L. on metabolism is still unknown. In order to test whether H. perforatum L. extract (EHP) has an effect on metabolic syndrome, we treated diet induced obese (DIO) C57BL/6J mice with the extract. The chemical characters of EHP were investigated with thin‐layer chromatography, ultraviolet, high‐performance liquid chromatography (HPLC), and HPLC‐mass spectrometry fingerprint analysis. Oral glucose tolerance test (OGTT), insulin tolerance test (ITT), and the glucose infusion rate (GIR) in hyperinsulinemic–euglycemic clamp test were performed to evaluate the glucose metabolism and insulin sensitivity. Skeletal muscle was examined for lipid metabolism. The results suggest that EHP can significantly improve the glucose and lipid metabolism in DIO mice. In vitro, EHP inhibited the catalytic activity of recombinant human protein tyrosine phosphatase 1B (PTP1B) and reduced the protein and mRNA levels of PTP1B in the skeletal muscle. Moreover, expressions of genes related to fatty acid uptake and oxidation were changed by EHP in the skeletal muscle. These results suggest that EHP may improve insulin resistance and lipid metabolism in DIO mice. © 2014 The Authors. Phytotherapy Research published by John Wiley & Sons Ltd.     

Antidepressant-like effect of peony glycosides in mice

AIM OF THE STUDY: The root part of Paeonia lactiflora Pall. (Ranunculaceae), known as peony, is often used in Chinese herbal formulae for the treatment of depression-like disorders. Previous studies in our laboratory have shown that an ethanol extract of peony produced antidepressive effects in mouse models of depression. It is well known that peony contains glycosides such as paeoniflorin and albiflorin, yet it remains unclear whether the total glycosides of peony (TGP) are effective. The present study aims to evaluate the antidepressant-like effects of TGP. MATERIALS AND METHODS: The antidepressant-like effects of TGP was determined by using animal models of depression including forced swim and tail suspension tests. The acting mechanism was explored by determining the effect of TGP on the activities of monoamine oxidases. RESULTS: Intragastric administration of TGP at 80 and 160mg/kg for seven days caused a significant reduction of immobility time in both forced swim and tail suspension tests, yet TGP did not stimulate locomotor activity in the open-field test. In addition, TGP treatment antagonized reserpine-induced ptosis and inhibited the activities of monoamine oxidases in mouse cerebrum. CONCLUSION: These results suggest that the antidepressive effects of TGP are mediated, at least in part, by the inhibition of monoamine oxidases.     

芍药苷和芍药内酯苷的抗抑郁作用与NO/cGMP信号转导通路的相关性

目的:比较赤芍、白芍及其有效成分芍药苷、芍药内酯苷对小鼠的抗抑郁作用及其与NO/c GMP信号转导通路的相关性。方法:选取小鼠悬尾实验模型,给予赤芍水提物、白芍水提物,记录小鼠悬尾不动时间。采用相同实验方法给予小鼠芍药苷和芍药内酯苷,并检测小鼠大脑皮质及海马组织中NO/c GMP通路相关指标。结果:与模型组比较,2 g/kg赤芍和2 g/kg、1 g/kg白芍组小鼠悬尾不动时间减少,差异有统计学意义(P0.05)。与2 g/kg赤芍同剂量组比较,白芍的小鼠悬尾不动时间减少,差异有统计学意义(P0.05)。与模型组比较,20 mg/kg芍药苷组、20 mg/kg芍药内酯苷组的小鼠悬尾不动时间及NO、c GMP含量明显减少,差异有统计学意义(P0.05);且与10 mg/kg芍药苷同剂量组比较,芍药内酯苷组的小鼠悬尾不动时间及NO含量明显减少,差异有统计学意义(P0.05)。与模型组比较,20 mg/kg芍药苷组及20mg/kg芍药内酯苷组的小鼠n NOS、GluR1mRNA表达明显减少,差异有统计学意义(P0.05)。结论:白芍具有抗抑郁作用,芍药苷和芍药内酯苷是白芍抗抑郁作用的物质基础;与白芍比较,赤芍的抗抑郁作用较弱,与其主要含有抗抑郁作用较弱的芍药苷,而不含抗抑郁作用较强的芍药内酯苷有关。     

Effects of Marapuama in the chronic mild stress model: further indication of antidepressant properties

ETHNOPHARMACOLOGY RELEVANCE: Ptychopetalum olacoides Bentham (PO) (Olacaceae), known as Marapuama, is regarded as a "nerve tonic" in the Amazon. Traditional uses include states of lassitude with noticeable lack of desire/motivation, and to manage particularly stressful (physical and/or psychological) circumstances. Suggestive of antidepressant activity, we have established that a specific PO ethanol extract (POEE) significantly decreases immobility in the tail suspension and forced swimming tests. AIM OF THE STUDY: The aim of this study was to verify the effects of POEE in the unpredictable chronic mild stress (UCMS) depression model in mice, given the construct and face values of the UCMS as an experimental model of depression and the traditional use of this species. MATERIALS AND METHODS: Over 6 weeks BALB/c mice were subjected to the UCMS protocol. The effects of POEE (50, 100, 300mg/kg, p.o.) and imipramine (20mg/kg, i.p.) were evaluated in relation to coat state, splash-test grooming, and corticosterone levels. RESULTS: The coat state degradation, decreased grooming and increased serum corticosterone induced by UCMS were prevented by POEE and imipramine treatments. CONCLUSION: In addition to supporting traditional claims and previously reported antidepressant properties for POEE, this study shows that POEE prevents stress-induced HPA hyperactivity.     

新型抗高血压复方制剂雅静胶囊降压及抗抑郁的研究

目的观察不同动物模型中新型复方制剂雅静胶囊的降压及抗抑郁作用。方法选用自发性高血压大鼠（SHR）模型、肾血管性高血压大只（RVHR）模型两种高血压动物模型,选取收缩压（SBP）、舒张压（DBP）、脉压（PP）及收缩压变异率（SBPV）4个指标观察雅静胶囊的急性降压作用。此外选用ICR小鼠通过悬尾实验和强迫游泳实验,选取不动时间和失望时间评价雅静胶囊的抗抑郁作用。结果在SHR动物模型的清醒状态下急性降压实验中,阳性药物厄贝沙坦及氢氯噻嗪组及3种不同剂量组的雅静胶囊急性给药后与给药前相比可显著降低大鼠的收缩压、舒张压．3组雅静胶囊的降压作用呈现明显的量效关系,对给药后记录8h的数据进行进一步分析提示：不同剂量雅静胶囊纽及阳性药物厄贝沙坦及氢氯噻嗪给药后0h～2h血压开始下降,在2h～4h时间段内降压效果达到最大并可维持8h．这些时间段内上述几组的SBP、DBP与对照组相比均显著下降（P〈0．05）,而PP和SBPV无统计学意义（P〉0．05）。RVHR的清醒状态下急性降压实验的结果基本类似。结论雅静胶囊能显著缩短小鼠悬尾实验的失望时间和强迫游泳实验的不动时间．提示雅静胶囊具有一定的抗抑郁作用,但该作用的量效关系不显著。