表皮细胞悬液移植治疗白癜风

目的 建立非培养的自体表皮细胞悬液移植治疗白癜风的方法,方法 治疗２４例稳定期白癜风患者。将取自臀部的皮肤体外消化后制成自体非培养的表皮细胞悬液,注入于白斑上冷冻诱发出的水疱内。结果 ２３例患者移植后显示色素恢复,１４例经１次移植后色素恢复达６２％～９３％;９例２次移植后色素恢复达８５％～９７％,除２例在色素出现１～３个月后又消退外,其余随访半年以上,色素保持良好。结论 非培养的自体表皮细胞悬液移     

自体表皮细胞移植加神经生长因子治疗白癜风

为了探讨神经生长因了（NFG）治疗白癜风的疗效，在自体表皮细胞移植的同时加用NFC观察35例白癜风患者的疗效，并与单纯使用自体表皮细胞移植病例对照，结果添加NFG的皮片全部存活，且色素恢复较快，色斑扩大更为显著，未见明显副作用，为目前治疗白癜风的一种更安全有效的治疗方法。     

非培养表皮细胞对比毛囊滤泡细胞悬液移植治疗稳定型白癜风的Meta分析

目的 通过Meta分析评价非培养表皮细胞悬液移植（NECS）和毛囊滤泡细胞悬液移植（HFCS）对稳定型白癜风复色显效率。方法 由两位研究员独立利用计算机检索pubmed,Cochrane,Web of Science数据库,收集有关NECS和HFCS的相关文献并进行筛选,采用Cochrane系统评价员手册对文章质量进行评价以及Review Manager 5.4软件进行数据分析。结果 纳入6篇临床随机对照试验,均为英文文献。其中NECS104例,HFCS99例。结论 目前根据患者选择及显效率更倾向于NECS技术,但还需要更多的随机对照试验（Randomized Controlled Trial,RCT）研究进行比较。     

自体表皮移植治疗白癜风112例临床观察

白癜风为一种常见病,治疗方法虽多,但对某些类型疗效不甚满意,１９８５年国外学者采用自体表皮移植治疗白癜风获得成功,表明黑素细胞可以通过表皮移植到白斑处,我科自１９９５年１１月～１９９９年４月采用自体表皮移植治疗白癜风病人 １１２例（４８７区）,取得满意疗效,现报告如下：１临床资料 １１２例均为本科门诊病人,男３９例,女73例;男女比为１：１．８７;年龄最小７岁,最大５９岁,平均２８．４岁;病程最短５月,最长３２年,平均３．５年;根据全国色素病学组１９９４年制订的白癜风分型（草案）标准,节段型 ５２例…     

自体表皮移植治疗白癜风102例临床疗效观察

2000年-2004年我科采用自体表皮移植法治疗白癜风患者102例，并进行了长期随访，临床上取得较满意的疗效，现报告如下。     

中药加自体表皮细胞移植治疗白癜风42例

笔者1998年1月～2004年6月采用中药内服外用，配合自体表皮细胞移植治疗白癜风42例，方法简单，疗效显著。     

自体表皮移植治疗进展期白癜风的临床观察

我们自2003年采用自体表皮移植结合药物治疗进展期白癜风79例，取得了较好的疗效。现报道如下。     

自体表皮移植术联合窄谱中波紫外线治疗白癜风临床疗效观察

我科自2004年3月-2005年3月采用自体表皮移植术联合窄谱中波紫外线（NB—UVB）治疗白癜风40例,收到满意效果,现报告如下。[第一段]     

自体表皮移植治疗白癜风55例疗效观察

我科于 1996年 4月～ 1998年 12月采用负压吸疱自体表皮移植治疗白癜风 55例 ,取得满意效果 ,现报告如下。临床资料 :白癜风患者 55例 ,男 4 8例 ,女 7例 ;年龄平均 2 1 6岁 (2 1～ 6 0岁 ) ;病程平均 6 3年 (2月～2 2年 )。局限型 2 8例 ,散发型 2 1例 ,肢端型 6例 ,其中 1例合并原发性甲状腺机能亢进症。以上患者病情均为稳定期 ,且多数曾用多种方法治疗无效。治疗方法 :器械采用本院自行研制的表皮分离仪和表皮磨削机。治疗前根据皮损情况部分病人简单备皮 ;治疗时供皮区根据病情选择 1～数个吸疱 ,直径0 5～ 1 0ｃｍ ,工作负压 4 0～ …     

自体表皮与黑素细胞移植治疗白癜风疗效对比

目的:研究和比较自体表皮移植与黑素细胞移植治疗白癜风的临床效果.方法:自体表皮移植采用局部皮肤发疱后直接进行移植;黑素细胞移植是从疱壁七获取黑素细胞、纯黑素细胞培养与增殖、移植区刮除种植法进行自体黑素细胞移植治疗.结果:自体表皮移植与黑素细胞移植治疗白癜风对其皮损的评分分别为(7.97±2.36)分和(11.46±2.57)分,经统计学分析差异有统计学意义(P＜0.01).结论:自体表皮移植操作方法较简单,黑素细胞移植可治疗面积大的皮损,而且色素分布均匀,临床效果更好.     

Rat marrow-derived multipotent adult progenitor cells differentiate into skin epidermal cells in vivo

Wound repair and functional reconstruction are two key aspects for treatment of skin injury. Research on cell source for skin repair has become a focus of study. The immune rejection induced by allograft cells and the limited source of autologous epidermal stem cells have led to more attention on the multipotent adult progenitor cells (MAPC). In this study, we examined the influence of the local environment of skin injury on the migration and differentiation of MAPC in nude mice. The homing of MAPC to the wounds and the epidermal differentiation of MAPC were investigated by detecting the expression of specific antigens of rat major histocompatibility complex I (MHC-I) antigen and the tracing markers. Three weeks after transplantation, hair follicle-like structure appeared and rat MHC-I antigen was positive in the follicles of the healed skin. PKH26-labeled cells expressing cytokeratin were found in the regenerated follicle-like structures, sebaceous glands and sweat glands. Our findings indicate that MAPC can migrate to the skin injury site and the hair follicles, and participate in skin wound healing by differentiating into epidermal cells, which contributes to the theoretical research of MAPC plasticity and provides theoretical evidence for clinical application of transplantation therapy with MAPC.     

浓缩生长因子作为生物敷料在稳定期节段型白癜风自体表皮移植术中的应用

 目的 观察浓缩生长因子在稳定期节段型白癜风自体表皮移植中应用的有效性及安全性。方法 纳入65例面部稳定期节段型白癜风患者,随机分为治疗组33例和对照组32例。所有患者均行自体表皮移植,治疗组移植区覆盖自体表皮后,加盖自体浓缩生长因子凝胶膜,然后凡士林纱布覆盖,无菌纱布加压包扎。对照组仅覆盖凡士林纱布。术后两周后,两组患者均行308 nm准分子光照射及外用0.1%他克莫司乳膏。规律随访至术后6个月。 结果 治疗后6个月,治疗组、对照组有效率分别为93.94%和75.00%,差异有统计学意义（X²=12.44,P=0.040) ;色素沉着发生率分别为9.09%和31.25%,差异有统计学意义 （X²=4.99,P=0.026);光晕现象发生率分别为15.15%和37.50%,差异有统计学意义（X²=4.20,P=0.040)。治疗组感染发生率为3.03%,对照组感染发生率为6.06%,差异无统计学意义 （X²<0.01,P=0.978) 。结论 浓缩生长因子作为生物敷料在节段型白癜风自体表皮移植中应用安全有效,值得临床推广。     

Optimization of murine keratinocyte culture for the production of graftable epidermal sheets.

The aim of the present study was to optimize murine epidermal cell cultures in order to obtain graftable sheets. Newborn (1-3 days old) Balb/c mice skin were used to optimize culture media and plating cell concentration, then epidermal sheet production, and grafting. Epidermal cells were plated at various concentrations in different culture media containing low (0.1 mM) or high (greater than 1 mM) Ca2+ levels. After a 3 day culture at the 10(4) cells/cm2 plating cell concentration, the percentage of differentiated cells was more than 80% in the high Ca2+ culture medium and less than 50% with bulky cells in the low Ca2+ culture medium. Under these conditions confluence was not obtained. At the 10(5) cells/cm2 seeding inoculum, the percentage of confluence increased to 95-100% during the first 72 h of culture in both high and low Ca2+ culture media. Three-day-old culture showed stratified multilayer epidermal sheets in the high calcium medium, and monolayer epidermal sheets were present in the low calcium medium after seeding keratinocytes in fibronectin precoated flasks. Seven days after plating, post confluent cultures were composed of a high percentage of differentiated cells (90%) with an increase in shedding cells in the medium. Considering the above morphological observations, sheets obtained with 10(5) cells/cm2 in MCDB-153 (A), DME-HAM (B) or GMEM (C) media after 3 days in culture were grafted. Twenty days after grafting, histological analysis of biopsies showed an epidermal structure and organization comparable to normal murine epidermis without hair follicles. Epidermal transplants showed a complete basement membrane, hemidesmosomes, and tonofilament bundles. Sheets obtained after seven day culture in all media showed lower coverage of the wound bed. These studies point out the importance of the plating cell and Ca2+ concentrations, and the culture time for murine keratinocyte confluence and differentiation to obtain graftable epidermal sheets.     

白癜风自体表皮移植处内皮素与干细胞因子的检测

目的 探讨白癜风自体表皮移植的疗效与局部细胞因子变化的相关性。方法 对稳定期白癜风患者进行负压吸疱移植治疗,部分患者移植前白斑曾进行窄谱中波紫外线照射,共57例患者成功收集到白斑及非白斑区疱液,并完成3个月随访,判断其疗效,其中照光者17例。用ELISA方法测定皮肤组织液中内皮素-1、干细胞因子的水平。结果 白斑区与非白斑区组织液自身对照比较,移植成功患者45例白斑区皮肤组织液内皮素-1、干细胞因子浓度分别为（728.97 ± 286.12） ng/L、（329.97±114.13） ng/L,非白斑区分别为（503.16 ± 251.44） ng/L、（224.73 ± 107.91） ng/L,白斑区与非白斑区比较,t值分别为5.44、5.90,P < 0.05。12例移植失败患者白斑区干细胞因子为（309.00 ± 163.89） ng/L,非白斑区为（204.22 ± 83.25） ng/L,两组比较,t ＝ 3.03,P < 0.05;而内皮素-1两组差异无统计学意义。移植成功患者在白斑区与非白斑区内皮素-1浓度明显高于移植失败患者,分别为（507.52 ± 283.31） ng/L和（344.91 ± 156.18） ng/L,t值分别为2.39,2.70,P < 0.05,干细胞因子浓度差异则无统计学意义。紫外线照射患者白斑区内皮素-1浓度与未照光患者比较,t ＝ 1.44,P > 0.05。在移植成功者中,行紫外线照射的15例内皮素-1为（548.48 ± 230.22） ng/L,未照光组为（794.60 ± 278.72） ng/L（P < 0.05）;干细胞因子浓度差异则无统计学意义。结论 内皮素-1、干细胞因子在白癜风的色素恢复中起作用,其中内皮素-1的作用可能更为重要。     

HA modulation of epidermal morphogenesis in an organotypic keratinocyte‐fibroblast co‐culture model

Please cite this paper as: HA modulation of epidermal morphogenesis in an organotypic keratinocyte‐fibroblast co‐culture model. Experimental Dermatology 2010; 19 : e336–e339. Abstract: Hyaluronan (HA) is a major extracellular matrix component of the skin. Amongst its biological functions is the maintenance of epidermal homeostasis. The mechanisms of action, however, remain unclear. To explore the interaction of HA with the epidermis, we have looked at the effects of exogenous application of HA in an organotypic culture model containing a dermal substrate with and without fibroblast incorporation. The results demonstrate that exogenous HA enhances epidermal proliferation resulting in a thicker viable epidermis with an increase in the number and intensity of Ki67‐positive basal cells; HA also improves the basement membrane assembly as evidenced by an increased expression of laminin‐332 and collagen type IV but not the expression of Nidogen‐1 at the epidermal–dermal junctional zone; furthermore, the development of epidermal lipid barrier structure was enhanced. These findings provide evidence to support the therapeutic use of exogenous HA for treating skin disorders with aberrant epidermal homeostasis.     

Effects of near-infrared radiation on the epidermal proliferation and cutaneous immune function in mice

While ultraviolet radiation alters various cutaneous cell functions, little is known about the photobiological effects of infrared radiation (IR) on the skin except its local thermal effect. This study demonstrated that single exposure of mouse skin to near IR (0.7-1.3 μm) reversibly suppressed the proliferating activity of the epidermis, the density of Langerhans cells, and the ability of skin to induce contact hypersensitivity reaction. During the exposure, the ear surface temperature was elevated from a mean of 27 to 31.2°C. The results suggest that near IR can modulate the epidermal proliferation and part of the skin immune system, with a mild thermal effect.     

Characterization of the expression and activation of the epidermal growth factor receptor in squamous cell carcinoma of the skin

BACKGROUND: Locally advanced skin cancers including squamous cell carcinoma (SCC) of the skin are increasing in incidence. Patients are often elderly with significant comorbidities and therapy can be difficult. New targeted therapies, such as treatment directed at the epidermal growth factor receptor (EGFR), may be effective and less toxic in these patients. However, before designing appropriate clinical trials it is necessary to characterize the expression and activation of targets such as the EGFR to evaluate the rationale of using EGFR inhibitors (EGFRIs) in the treatment of this type of cancer. OBJECTIVES: To characterize the expression and activation by phosphorylation of EGFR in SCC of the skin by quantitative Western blotting using the LiCor immunofluorescence detection system with validation by immunohistochemistry. Secondary objectives were to evaluate downstream targets of EGFR expression and activation in SCC of the skin and to examine the associations between EGFR, pathological features and clinical behaviour of these tumours. METHODS: Twenty-one mainly locally advanced skin SCCs collected in our institution and stored in our tissue bank over a 4-year period were used for the study. RESULTS: Nine of 21 (43%) tumours expressed EGFR above background. Of those nine, five expressed phosphorylated EGFR. There was no correlation with downstream activation of canonical signalling pathways, pathological features or clinical behaviour. CONCLUSIONS: EGFR is expressed in a minority of tumours and then is not always activated. These results show that, before designing a trial with a targeted agent such as an EGFRI in SCC of the skin, it is important to verify the presence of the appropriate target to maximize the best outcome.