Immunogold-Silver Staining Overview

Abstract

Silver stains are commonly used in histology laboratories to demonstrate a variety of tissue substances. With the passage of time. it has become evident that several of the chemicals used in silver stains, as well as silver compounds themselves, pose hazards to laboratory workers. Disposal of these chemicals also poses a problem.

This article briefly outlines some of the hazards of working with the chemicals used in silver stains. A comprehensive chemical hygiene plan, which must be developed for each laboratory, provides a standard operating procedure for working safely with chemicals. Material safety data sheets provide information on each chemical used in the laboratory and must be available. The use of several of the chemicals employed in silver stains is covered by Federal, state, and local laws. The state and local laws may be more stringent than the Federal laws. (The J Histotechnol 19:197, 1996)     

Rapid Immunoperoxidase Staining

Abstract

With the aid of moist heat and a magnetic stirrer, a method for rapidly performing immunoperoxidase procedures has been devised. With this method, approximately one half the time is required, with equally dependable results.     

Consistent Warthin-Starry Staining Technique

Abstract

A consistent ¹procedure for the Warthin-Starry stain technique for melanin and spirochetes is described. Close attention to solution preparation, purity, and pH, freshness of chemicals used, and rigorous adherence to the protocol is essential. The procedures and recommendations described ensure consistent success when using this often difficult stain technique. (The J Histotechnol 19:339–340, 1996)     

Staining bacterial flagella easily.

A wet-mount technique for staining bacterial flagella is highly successful when a stable stain and regular slides and cover slips are used. Although not producing a permanent mount, the technique is simple for routine use when the number and arrangement of flagella are critical in identifying species of motile bacteria.     

Enhancement of the Paraldehyde-Fuchsin Staining

Abstract

We have modified the aldehyde-fuchsin (PAF) stain to enhance its ability to simultaneous demonstration of different tissue compounds. We have substituted the light green dye of Hallni mixture with fast green dye and extended the reduction time by oxalic acid. The resulting stain provides better visualization of several tissue elements, such as the distinction between the nucleus and secretion products of goblet cells and between elastic and collagen fibers and others tissue elements. (The J Histotechnol 21:147, 1998)     

对比快速染色法与革兰氏染色法对阴道炎的诊断价值

目的 对比分析快速染色法与革兰氏染色法在阴道分泌物涂片检查中的应用效果及在阴道炎诊断中的价值.方法 回顾性选取北京妇产医院2019年10月至2019年11月疑诊的阴道炎患者120例作为研究对象(其中116例确诊为阴道炎),以无菌棉拭子采集患者阴道分泌物并制作成2组样本,分别进行快速染色法与革兰氏染色法检测.根据临床症状、体征、实验室结果 做出临床诊断.统计快速染色法与革兰氏染色法对病原体检出的数量、灵敏度、特异性、准确率及两种方法的经济成本与效率,分析它们之间的差异.结果 120例患者中,革兰氏染色法与快速染色法分别检出病原体101、97例,其中线索细胞分别为52、48例,真菌分别为30、31例,滴虫均为2例,混合感染分别为17、16例,2种方法各病原体检出率差异无统计学意义(均P>0.05);且120例疑诊阴道炎患者经确诊后,阴道炎116例,非阴道炎4例.快速染色法与革兰氏染色法检出阴道炎分别为97与101例,非阴道炎分别为23与19例,与确诊结果 一致数分别为101与105例;同时2种检测方法病原体检出的灵敏度、特异性、准确率比较,差异均无统计学意义(均P>0.05);且快速染色法的经济成本更低,操作时长更短.结论 快速染色法检测结果 准确、灵敏,特异性好,且与革兰氏染色法检测结果 对比差异无统计学意义,但经济成本更低,操作时长更短,效率更高.