自血光量子疗法和腹蛇抗栓酶联合治疗兔脊髓损伤

４８只兔造成脊髓损伤，分为四组：对照组、腹蛇抗栓酶（ＡＡ）治疗组、自血光量子疗法（ＡＬＱ）治疗组以及ＡＡ和ＡＬＱ联合治疗组．通过运动功能、脊髓诱发电位、光镜和电镜的对比研究，发现ＡＬＱ和ＡＡ联合应用的疗效优于ＡＬＱ或ＡＡ单独应用．认为ＡＬＱ和ＡＡ联合应用除了都能改变血液流变学性质外，ＡＬＱ可以提高组织供氧量和氧利用率，ＡＡ可以溶栓疏通微循环，因此具有促进脊髓功能恢复的协同疗效。     

自血光量子疗法治疗家兔脊髓损伤的形态学观察

自血光量子疗法治疗家兔脊髓损伤的形态学观察２３００３２安徽医科大学病理张王海，王道斌，张红安徽医科大学附院骨科董英海１９９０年起本校附院应用自血光量子疗法（ＡＬＱ）治疗脊髓损伤，取得了明显疗效［１，２］，随后进行了一系列的基础研究［３，４］。现就ＡＬ...     

自血光量子疗法治疗脊髓损伤的兔脊髓血流量变化观察

自血光量子疗法治疗脊髓损伤的兔脊髓血流量变化观察２３００２２安徽医科大学附属医院张循善，董英海，吴基，邵宏英，陈晓燕，卫玉芝，程越１９９０年起，笔者在临床尝试应用自血光量子疗法（ＡＬＱ）治疗脊髓损伤，取得了满意疗效［１］并开展了一系列基础研究［２，３...     

光量子自血疗法治疗脑血管病102例疗效观察

光量子自血疗法治疗脑血管病１０２例疗效观察河南省信阳地区人民医院内科（４６４０００）胡法富，杨茂轩光量子自血疗法又称紫外线照射及充氧后的自血回输疗法。我科用光量子自血疗法治疗脑血管病（ＣＶＤ）１０２例，疗效满意，报道如下。１资料与方法１．１病例：１９...     

自血光量子疗法和腹蛇抗栓酶联合治疗兔销髓损伤

４８只兔造成脊髓损伤，分为四组：对照组、腹蛇抗栓酶治疗组、自血光量子疗法治疗组以及ＡＡ和ＡＬＱ联合治疗组。通过运动功能、脊髓诱发电位、光镜和电镜的对比研究，发现ＡＬＱ和ＡＡ联合应用的疗效优于ＡＬＱ或ＡＡ单独应用。认为ＡＬＱ和ＡＡ联合应用除了都能改变血液流变学性质外，ＡＬＱ可以提高组织供氧量和氧利用率；ＡＡ可以溶栓疏通微循环，因此具有促进脊髓功能恢复的协同疗效。     

自血光量子疗法治疗破伤风的护理体会

自血光量子疗法治疗破伤风的护理体会周红，沈汉斌，胡仲武，赵亚敏自血光量子疗法又称紫外线照射和充氧自体血回输疗法，是经过专门的治疗机，将病人的静脉血经体外抗凝、紫外线照射和充分充氧后立即经静脉一次回输给病人，以达到治疗目的（以下简称血疗）。血液经紫外线...     

自血光量子疗法与光化学疗法治疗银屑病109例

自血光量子疗法与光化学疗法治疗银屑病１０９例李平安银屑病是一种病程经过缓慢、易复发的慢性疾病，通常采用以“光化学疗法”（ＰＵＶＡ）为主的综合治疗方法，虽有一定的疗效，但疗程长，易复发。为探索更优疗法，我院采用以自血光量子疗法（简称“血疗”）为主的综...     

血浆置换加自血光量子疗法促醒肝昏迷2例报告

笔者采用血浆置换术（ＰＥ）加自血光量子疗法（ＡＬＱ）成功促醒２例肝昏迷患者，现报告如下。１病例介绍１．１例１，男，４６岁，因精神错乱，极度烦燥，不识家人，间歇昏睡于１９９６年９月２９日入院。患者有３０多年饮酒史，肝肿大，肝功能损害，于３年前确诊为酒精...     

异体血光量子疗法治疗慢性肾功能衰竭的临床及免疫学研究

目的：探索慢性肾功能衰竭（ＣＲＦ）的有效治疗方法。方法：应用异体血光量子疗法治疗ＣＲＦ患者１４例（治疗组），并与对照组（１２例）作疗效对比。结果：治疗组在降低血尿素氮（ＢＵＮ）和血肌酐（ＳＣｒ）、增加尿量和内生肌酐清除率（ＣＣｒ）及提高ＣＤ＋４、ＣＤ＋４／ＣＤ＋８比值等方面均明显优于对照组，具有显著性差异（Ｐ均＜０．０５），且患者的恶心、呕吐症状明显改善，而血压变化不大。结论：异体血光量子疗法治疗ＣＲＦ的疗效可靠、安全，疗效优于常规治疗。     

自血光量子疗法加血液稀释应用于脑血管疾病的疗效观察

自血光量子疗法（简称血疗）应用于脑血管疾病，有一定疗效，并通过流变学研究证实了紫外线辐照血液后，可使红细胞聚集性下降，血氧饱和度增加［１］；血液稀释疗法（简称血稀），在降低红细胞压积，降低血粘度方面有显著的疗效。笔者采用血疗加血稀治疗脑血管恢复期病人...     

多普勒超声对自血光量子疗法治疗兔脊髓损伤的肢体血流状态的研究

24只家兔造成胸腰段脊髓损伤，用多普勒超声研究损伤平面上下的血流速度频谱，发现脊髓损伤后腋动脉血流峰值无变化，而股动脉明显慢。白血光量子疗法（ALQ）能提高股动脉血流峰值，提示ALQ可以增加损伤平面以下的血流供应，改善脊髓缺血状况，有利于脊髓功能的恢复。     

Neurophysiological characterization of the anterolateral spinal cord neurons contributing to pain perception in man

These studies have examined threshold, frequency, and refractory period characteristics of a neural population in the anterolateral quadrant (ALQ) of the spinal cord of man, stimulation of which produces pain. Subjects were 18 conscious humans undergoing percutaneous anterolateral cordotomy for relief of intractable pain. Pain could be produced by ALQ stimulation in all subjects. Pain thresholds ranged from 120 to 1000 μA (at 50 pulses/sec; 0.2 msec pulses), but the majority of thresholds were below 300 μA. A linear relationship was found between stimulation frequency and percentage of subjects reporting pain. This relationship ranged from 5 to 25 pulses/sec with 100% reporting pain at 25/sec and 0% at 5/sec. In 2 of 3 subjects, increases in stimulation frequency up to 500/sec did not produce pain when stimulation intensity was below threshold at 50/sec. The neuronal refractory period for pain in these subjects ranged between 1.0 and 2.0 msec, but the majority of relative refractory periods fell between 1.0 and 1.5 msec. The threshold, frequency and refractory period data obtained in this study are similar to those found for wide dynamic range cells in the ventral half of the dorsal horn in the monkey and suggest that activation of these cells is a sufficient condition to produce pain in man.     

Neurophysiological characterization of the anterolateral quadrant neurons subserving pain in M. Mulatta

An electrophysiological analysis has been made of 82 L₇ dorsal horn neurons antidromically activated from the contralateral C₁ anterolateral quadrant (ALQ) of unanesthetized rhesus monkeys (bilateral carotid ligation). This analysis was made to compare refractory periods and antdromic activation thresholds with these same parameters of ALQ stimulation required to produce pain in conscious humans. Refractory periods of laminae IV-VI cells that were optimally but not exclusively responsive to noxious skin stimulation ranged from 0.8 to 2.8 msec ($\text{m}\text{= 1.5}$) and were briefer than those of lamina I cells. The latter ranged from 1.1 to 10 msec ($\text{m}\text{= 4.7}\text{msec}$). Electrical thresholds of laminae IV-VI cells were, in general, much lower than those of laminae I cells. Unlike lamina I cells, refractory periods and electrical thresholds of laminae IV-VI nociceptive neurons closely parallel those of ALQ-evoked pain in man. However, both lamina I and laminae IV-VI neurons usually responded to nociceptive skin temperatures (> 43 °C). This analysis indicates that pain may be signaled by the combined output of dorsal horn laminae I and IV-VI but that activation of only laminae IV-VI wide dynamic range neurons is sufficient to produce pain.     

The primate dorsal spinothalamic tract: evidence for a specific termination in the posterior nuclei (Po/SG) of the thalamus.

The spinothalamic tract in primates and other mammals arises primarily from cells in lamina I of the dorsal horn, from lamina V cells and to a lesser extent from other laminae. Most of the neurons of lamina I respond only to noxious mechanical or thermal stimuli. Spinothalamic tract (STT) cells of lamina V tend to respond to both innocuous and noxious stimuli. Recent studies have suggested that the classical STT in the anterolateral quadrant (ALQ) contains primarily the axons of lamina V cells and that the axons of lamina I cells travel more dorsally in the dorsolateral quadrant (DLQ) to constitute the dorsal spinothalamic tract (DSTT). Using the anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) injected into the spinal cord in conjunction with a contralateral anterolateral cordotomy, we have found there is a substantial projection of the DSTT to the posterior nuclei of the caudal-ventral thalamus, designated Po/SG. This projection is almost entirely abolished when the lesion includes the area of spinal cord white matter at the level of the denticulate ligament. Larger lesions that destroy the ALQ and much of the lateral column white matter, but that spare the dorsolateral column white matter in the region of the corticospinal tract, abolish all transport of WGA-HRP to the thalamus. We conclude that the spinothalamic pathway in the non-human primate encompasses a continuous fiber bundle that extends dorsally to include the region of lateral column white matter opposite the denticulate ligament and that the more dorsal aspect of this pathway projects primarily to Po/SG of thalamus.     

Saline infusion sonohysterography and the risk of malignant extrauterine spread in endometrial cancer

The aim of this study was to evaluate the incidence of tumor cell dissemination after saline infusion sonohysterography (SIS) in patients with endometrial cancer (EC). A retrospective study was conducted on 173 patients with EC in whom one of the following methods were performed: dilation and curettage (D&C, n = 120) and D&C followed by SIS (n = 53). No selection or randomization of patients was applied to both groups. The presence of positive peritoneal cytology, as well as adnexal or abdominal metastases was considered the endpoint of this analysis. Positive peritoneal cytology was present in one patient (0.8%) after D&C and in one patient (1.9%) after D&C followed by SIS (chi(2) = 0.030; p > 0.05). Adnexal metastases were present in nine (7.5%) patients after D&C and in three (5.7%) patients after D&C followed by SIS (chi(2) = 0.013; p > 0.05). Metastases to abdominal cavity were found in three (2.5%) patients after D&C and in two (3.8%) patients after D&C followed by SIS (chi(2) = 0.001; p > 0.05). Upstaged because of positive cytology, adnexal or abdominal metastases were 13 patients (10.8%) after D&C and six patients (11.3%) after D&C followed by SIS (chi(2) = 0.029; p > 0.05). These data show that SIS does not increase the risk of malignant cell dissemination in patients with EC.     

Effects of Renal Failure and Dialysis on Cefazolin Pharmacokinetics

Serum and urinary levels of cefazolin were determined after a 500-mg parenteral dose in eight azotemic volunteers. The mean peak serum concentration was 1.5 to 5 times the levels obtained in nonazotemic patients. The serum half-life of cefazolin was increased significantly. In patients on dialysis, the mean serum half-life of cefazolin was 4.05 h during (or after) hemodialysis, and 32.1 h during (or after) peritoneal dialysis. There was a significant decrease in cefazolin removal when dialysate flow or membrane surface area of the dialyzer were decreased. It was also shown that one circuit through the dialysis unit caused measurable decrease in cefazolin concentration. These data and previously published reports suggest: (i) the maintenance dose of cefazolin can be decreased in azotemic patients; (ii) patients on hemodialysis will require an additional half dose after dialysis because of efficient removal during hemodialysis; and (iii) patients on peritoneal dialysis do not require an extra dose.     

EBV-DNA、T-钙黏蛋白和PCNA在鼻咽癌患者血清中的表达及其临床意义

[目的]探讨EB病毒(EBV)-DNA、T-钙黏蛋白(T-cadherin)和增殖细胞核抗原(PCNA)在鼻咽癌患者血清中的表达及其临床意义.[方法]选取2013年1月至2015年12月在本院收治的150例晚期(Ⅲ~Ⅳa期)鼻咽癌患者作为鼻咽癌组,另外选取同期在本院诊治的50例慢性鼻炎(鼻炎组)和体检的健康志愿者50例(对照组).采用实时荧光定量PCR检测治疗前后EBV-DNA、T-cadherin mRNA和PCNA mRNA水平;随访2年,记录复发转移情况,并分析其与以上检测指标的关系.[结果]鼻咽癌组、鼻炎组和对照组三组在EBV-DNA阳性率、T-cadherin mRNA和PCNA mRNA比较差异有统计学意义(P<0.05);与治疗前比较,鼻咽癌组治疗后PCNA mRNA水平明显降低(P<0.05),T-cadherin mRNA明显升高(P<0.05).150例鼻咽癌患者中治疗敏感(TS)124例,TR者26例;治疗前,治疗抵抗(TR)者EBV-DNA阳性率和PCNA mR-NA均高于TS者,而则T-cadherin mRNA低于TS者,差异均具有统计学意义(P<0.05).150例鼻咽癌放疗后患者,其中35例出现复发转移,复发转移率23.33%,其中3例复发,32例远处转移.转移者EBV-DNA阳性率明显高于无复发转移者(P<0.05),无复发转移者T-cadherin mRNA高于复发者和转移者(P<0.05),无复发转移者PCNA mRNA低于复发者和转移者(P<0.05).[结论]EBV-DNA、T-cadherin和PCNA在晚期鼻咽癌患者放疗前后及放疗后复发转移中差异表达,有望成为疗效及预后评估的有效指标.     

Antipyrine kinetics in liver disease and liver transplantation

Antipyrine kinetics were studied in seven normal subjects, 10 patients with liver disease, and 13 clinically stable patients who received a liver transplant. Five patients were studied both before and after liver transplantation. Antipyrine concentrations in saliva after oral dosing were measured by HPLC. The antipyrine t1/2 was significantly longer (P less than 0.05) in patients with liver disease than in patients undergoing liver transplantation and normal subjects. Antipyrine clearance was not significantly different between patients undergoing liver transplantation and normal subjects, but it was significantly reduced (P less than 0.05) in patients with liver disease. In five patients who were studied before and after liver transplantation, there was a significant (P less than 0.05) increase in the antipyrine clearance and a marked reduction in its t1/2 after liver transplantation. These results indicate that liver transplantation improves the drug metabolizing ability of patients with liver disease and that the oxidative metabolizing capacity of the liver in clinically stable patients after liver transplantation is similar to that of normal subjects.     

心肌存活及灌注对急性心肌梗死冠状动脉介入治疗术后患者心功能的影响

目的探讨急性心肌梗死冠状动脉介入治疗(PCI)术后患者梗死区心肌存活性对左室重构及功能的影响。方法 2006年2月-2010年12月208例急性心肌梗死急诊PCI术后的患者接受静息状态下18氟-脱氧葡萄正电子断层显像进行心肌代谢显像检查,根据基线梗死区心肌有无存活分为两组,同时进行超声心动图检查,评价左室壁运动、左室射血分数,左室舒张末内径、左房内径及舒张期二尖瓣血流速度峰值的比值。血运重建术后12个月随访超声心动图,观察梗死区心肌存活状态对于左室重构以及心功能的影响。结果 PCI术后12个月,有存活心肌组左室射血分数(46.7±6.98)%高于无存活心肌组(45.1±7.12)%,两组差异有统计学意义(P<0.01),有存活心肌组左室舒张末期内径(53.17±3.89)mm小于无存活心肌组(55.46±4.75)mm,两组差异有统计学意义(P<0.05)。左房内径及舒张期二尖瓣血流速度峰值的比值两组随访时均无明显变化。结论急性心肌梗死行PCI治疗后的患者,在有存活心肌的情况下,心功能改善明显;而梗死区无心肌存活的患者,12个月后,心功能减低,左室重构更加明显。