Thirty-two polymorphic microsatellite loci of the mysid crustacean Mesopodopsis tenuipes

Thirty-two polymorphic microsatellite loci were indentified and characterized from the mysid Crustacea Mesopodopsis tenuipes Hanamura et al., 2008. The number of observed alleles per locus in 32 individuals collected from its type locality in Malaysia ranged from 2 to 22. The observed heterozygosity ranged from 0.031 to 0.906, while the expected heterozygosity varied from 0.031 to 0.943. All loci conformed to Hardy?CWeinberg equilibrium, no linkage disequilibrium was observed between pairs of loci and no loci showed evidence of null alleles. These 32 microsatellite loci could be used in future population genetics study of M. tenuipes.     

Characterization of ten polymorphic microsatellite markers in an invasive Ponto-Caspian mysid crustacean Paramysis lacustris

Paramysis lacustris is a Ponto-Caspian mysid crustacean species. It inhabits coasts, estuaries and lower parts of rivers in the Black, Azov and Caspian seas and shows high levels of genetic structuring and cryptic diversity. The species has been introduced into the Baltic Sea basin and is considered to be a high risk invader. We developed ten microsatellite loci and tested their polymorphism in 70 individuals from four wild populations. The number of alleles per locus ranged from 4 to 23; none of the loci showed consistent deviations from Hardy–Weinberg equilibrium. These microsatellite markers will be useful to trace potential invasions and understand dynamics of native P. lacustris populations.     

Development of polymorphic microsatellite markers for the Pleuroderma thaul

Pleurodema thaul (Leptodactylidae) is a small frog from Chile and Argentina with a distributional range that spans more than 2,500 km. We isolated and characterized a total of 23 microsatellite loci from 24 individuals. The number of alleles per locus ranged from 7 to 22, observed heterozygosity ranged from 0.417 to 0.958, and the probability of identity values ranged from 0.0064 to 0.21.     

Development and characterization of 42 novel polymorphic microsatellite markers for Odontobutis potamophila from EST sequences

Expressed sequence tags (ESTs) are sources for microsatellite development. In this study, we isolated and characterized microsatellite makers for Odontobutis potamophila using next-generation sequencing. A total of 42 polymorphic microsatellite repeat markers were identified in 32 individuals from Dangtu wild population. The number of alleles per locus varied from 2 to 10, and the observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.061 to 0.878, respectively. These EST-SSR makers would provide sufficient polymorphism for population genetic studies, parentage and genome mapping of O. potamophila and its closely related fishes.     

Isolation and characterization of 18 novel polymorphic microsatellite markers from the Mayan cichlid (Cichlasoma urophthalmus)

We isolated and characterized 18 microsatellite loci from the Mayan cichlid, Cichlasoma urophthalmus. Loci were screened for 24 specimens from a total of seven sites in south Florida, Mexico, Belize and Honduras. The number of alleles per locus ranged from 3 to 21, observed heterozygosity ranged from 0.208 to 0.875, and the probability of identity values ranged from 0.012 to 0.203. These new loci will provide tools for identifying the source population(s) for the introduction of Mayan cichlids in south Florida and for comparing population genetic structure of Mayan cichlids within and among subpopulations in its native (Central America) and introduced ranges (south Florida). Mayan cichlids are an invasive species in south Florida so identifying source populations may reveal pathways that can be managed to prevent further introductions. Mayan cichlids may also be useful as a model system in which to examine the relationship between introduction history, population genetic diversity, and invasibility.     

Development of polymorphic microsatellite markers for the bonnethead shark,Sphyrna tiburo

Conservation Genetics Resources - We isolated and characterized a total of 22 microsatellite loci from Sphyrna tiburo. Loci were screened in 24 individuals from St. Catherine’s Island,...     

Development and characterization of 23 polymorphic microsatellite markers for Sympetrum frequens

Twenty-three polymorphic microsatellite markers for Sympetrum frequens were developed and characterized. The number of distinct alleles per locus in 32 individuals ranged from 2 to 23. The observed heterozygosity ranged from 0.031 to 0.938, while the expected heterozygosity varied from 0.031 to 0.922. No loci deviated significantly from Hardy?CWeinberg equilibrium, no linkage disequilibrium was observed between pairs of loci and no loci showed evidence of null alleles. These microsatellite markers are expected to contribute to future investigations of genetic variation and structure in S. frequens populations.     

Thirteen novel polymorphic microsatellite markers for endangered Chinese endemic herb Dendrobium Officinale

We isolated and developed 13 novel microsatellite makers from expressed sequence tag sequences of endangered Chinese endemic herb Dendrobium officinale and examined them for 38 individuals from Jinhua, Zhejiang, Chian. These loci displayed the mean number of 4. 31 alleles per locus, and with expected heterozygosity (H _e) and observed heterozygosity (H _o) per locus ranged from 0.4438 to 0.6842 and from 0 to 0.4211, respectively. These markers have potential for application in germplasm appraisal, genetic diversity study, genetic mapping, and molecular breeding in D. officinale and other congeneric species.     

Development of eleven polymorphic microsatellite markers for the Chaco Treefrog, Hypsiboas raniceps

We report the characterization of eleven polymorphic microsatellite loci for Hypsiboas raniceps in 20 individuals collected in the northwest of São Paulo State, Brazil. The number of alleles per locus, observed heterozygosity and polymorphic information content ranged from 2 to 16, 0.05 to 0.90 and 0.048 to 0.905, respectively. Linkage equilibrium was observed for all loci and only one locus showed significant deviation from Hardy–Weinberg equilibrium with evidence of null alleles. The remaining ten loci will be useful for population genetic studies and revealing population structure in this species.     

Development of polymorphic microsatellite markers for the orange-breasted falcon (Falco deiroleucus)

We isolated and characterized a total of 24 microsatellite loci from the orange-breasted falcon (Falco deiroleucus). Loci were screened in 32 individuals currently maintained as a captive population: 13 wild-caught individuals from Panama and four from Belize, and the remaining 15 were first generation F1 offspring. The number of alleles per locus ranged from 2 to 9, observed heterozygosity ranged from 0.000 to 0.938, and the probability of identity values ranged from 0.075 to 0.604. These new loci will provide tools for identifying relatedness values among sampled individuals currently in captivity and for exploring genetic diversity levels and connectivity between populations in Central America.     

Isolation and characterization of polymorphic microsatellite markers in the fish Garra orientalis (oriental sucking barb)

14 Polymorphic microsatellite markers were developed in the Garra orientalis. These new markers were tested in 23 individuals collected from the Wanquan River in the Hainan Island. The number of alleles (N_A), polymorphism information content, observed and expected heterozygosity per locus ranged from 8 to 25, 0.6788 to 0.9342, 0.5652 to 1.0000 and 0.7179 to 0.9585, respectively. Four loci are deviated from Hardy–Weinberg equilibrium due to the heterozygote deficiency. These markers can be used to study genetic diversity, population structure, conservation and rational utilization of this species.     

Development of polymorphic microsatellite markers for Ginkgo biloba L. by database mining

Ginkgo biloba L. is a famous living fossil plant endemic to China. We report twelve polymorphic simple sequence repeat (SSRs) markers of G. biloba by mining expressed sequence tags (ESTs). One locus was significantly deviated from Hardy-Weinberg equilibrium. The number of alleles per locus ranged from 2 to 5. The PIC values were from 0.058 to 0.776. Observed and expected heterozygosity ranged from 0.050 to 0.776, and 0.055 to 0.799, respectively. These markers will be available for studies of population genetics, reproductive ecology and conservation genetics for G. biloba.     

Development of polymorphic microsatellite markers for blue king crab (Paralithodes platypus)

We isolated and characterized a total of 23 microsatellite loci from the blue king crab, Paralithodes platypus. Loci were screened in 24 individuals from St. Matthew Island. The number of alleles per locus ranged from 4 to 17, observed heterozygosity ranged from 0.050 to 1.000, and the probability of identity values ranged from 0.015 to 0.339. These new loci will provide tools for examining the genetic population structure of the species throughout its range.     

Development of 39 novel polymorphic microsatellite markers for the giant mottled eel Anguilla marmorata and cross-amplification in other eel species

In this study, we have developed microsatellite markers for the giant mottled eel (Anguilla marmorata) from expressed sequence tag (EST) sequences using next-generation sequencing. Consequently, a total of 39 novel polymorphic microsatellite markers were identified in 32 individuals from Wangquan River wild population area. The range of observed heterozygosity, expected heterozygosity and polymorphism information content were 0.044–1.000, 0.285–0.946 and 0.3783–0.9016, respectively. In addition, eight loci were deviated from Hardy–Weinberg equilibrium and modified by Bonferroni correction, and the results showed no significant linkage disequilibrium between the locus pair. The amplification of cross-species was conducted in Anguilla bicolor pacifica and Anguilla japonica, which revealed the applicability of EST-SSR primers. These EST-SSR markers can provide sufficient polymorphism for population genetic studies, pedigree and genome mapping of these three kinds of closely related eels.     

Development of nineteen novel polymorphic microsatellite loci of Clupeichthys aesarnensis

We developed and characterized a total of 19 novel polymorphic microsatellite loci from the genome of Clupeichthys aesarnensis. Flanking PCR primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 30 individuals ranging from two to 15. The observed heterozygosity ranged from 0.033 to 0.933, while the expected heterozygosity varied from 0.033 to 0.898. All loci conformed to the Hardy–Weinberg equilibrium, none of the loci combinations showed significant linkage disequilibrium, and no loci showed evidence of null alleles. These 19 microsatellite loci can be useful for conducting future investigations into the genetic differentiation and structure of populations of C. aesarnensis.     

Eleven highly polymorphic microsatellite markers for the lizard Anolis distichus

We isolated and characterized 11 polymorphic microsatellite loci for Anolis distichus, a West Indian lizard endemic to Hispaniola and the Bahamas. The number of alleles per locus ranged from 10 to 34, with levels of observed heterozygosity ranging from 0.10 to 1.00. Four loci significantly deviated from Hardy–Weinberg equilibrium for most of the populations sampled, and seven pairs of loci demonstrated linkage disequilibrium for 1–2 populations. These markers provide a valuable set of tools for fine-scale population genetics studies of A. distichus, a species that exhibits striking geographic variation in dewlap coloration and pattern.     

Development and characterization of thirteen polymorphic microsatellite markers in the fish Osteochilus salsburyi

Osteochilus salsburyi (Cypriniformes, Cyprinidae) is a small-sized fish of significant economic value. In this study, thirteen polymorphic microsatellite markers were developed in O. salsburyi and were examined on twenty individuals collected from the Wanquan River in the Hainan Island. The number of alleles (N_A), polymorphism information content, observed and expected heterozygosity per locus ranged from 10 to 21, 0.8020 to 0.9354, 0.7500 to 1.0000 and 0.8436 to 0.9628, respectively. No locus was found to significantly deviate from Hardy–Weinberg equilibrium. These markers would be suitable for studies on conservation genetics and population structure of O. salsburyi.     

Development of polymorphic microsatellite markers for the microendemic pupfishes Cyprinodon julimes and C. pachycephalus

We developed microsatellite loci for the Julimes pupfish, Cyprinodon julimes. Twenty-five loci were screened across 19 individuals from Julimes Spring, Chihuahua, Mexico. The number of alleles per locus ranged from 2 to 14, observed heterozygosity ranged from 0.105 to 0.947, and the probability of identity values ranged from 0.022 to 0.588. We then tested for cross-amplification in the bighead pupfish, C. pachycephalus; twenty-three individuals from San Diego de Alcalá, Chihuahua, Mexico, were screened across the 20 loci that amplified cleanly. These new loci will be used for long-term genetic monitoring of these critically endangered species.