心可舒胶囊中葛根素的含量测定

目的:测定心可舒胶囊中葛根素的含量.方法:采用高效液相色谱法(HPLC法),色谱柱用C18柱,流动相为甲醇-水(85:15),流速为1.0ml/min,波长为250nm.结果:葛根素在1～5μg/ml范围内浓度与峰面积线性关系良好.平均回收率为98.89%.结论:该法操作简便,结果准确,可用于心可舒胶囊中葛根素的含量测定.     

高效液相色谱法测定降糖舒分散片中葛根素的含量

目的 建立高效液相色谱法测定降糖舒分散片中葛根素的含量.方法 色谱柱:Novo-PakC18柱(250 mm×3.9 11mm,4 μm);流动相为甲醇:水(25∶75),检测时波长:250 nm;柱温:30℃;流速:0.8 ml/min;理论塔板数按葛根素计算不低于2000.结果 葛根素0.0927～1.854 μg时呈良好的线性关系(r=0.9999),平均回收率为100.47%,RSD=1.11%.结论 本法简便、快捷、结果准确,可作为降糖舒分散片的质量控制标准.     

RP—HPLC测定益脑复健胶囊中葛根素的含量

目的:建立测定益脑复健胶囊中葛根素含量的高效液相色谱法.方法:采用反相高效液相色谱法,使用Hy-persil BDS C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸(19 ml:81 ml),流速为0.8 ml/min,检测波长为250 nm,柱温为30℃,采用外标法.结果:葛根素在0.156～0.936μg范围内线性关系良好,r=0.999 1;平均加样回收率为99.0%,RSD为0.6%(n=6).结论:本方法简便、结果准确、重现性好,可用于益脑复健胶囊的质量控制.     

RP-HPLC法测定心可舒片中葛根素的含量

[目的]建立心可舒片中葛根素的HPLC含量测定方法。[方法]高效液相色谱法;色谱条件:ZORBAX Eclipse XDB-C18,柱温25摄氏;流动相:甲醇-水-磷酸(23∶77∶0.5);流速:1.0 ml·min-1;检测波长;250nm。[结果]葛根素在25ng~250ng范围内,线性关系良好,回归方程为Y=4663.9774X(r=0.9998),平均加样回收率为102.34%,RSD=2.7197%(n=5)。[结论]本方法稳定、可靠、操作简便、快速、重现性好,可用于心可舒片葛根素的质量控制。     

HPLC测定丹葛颈舒胶囊中葛根素的含量

目的：建立测定丹葛颈舒胶囊中葛根素的高效液相色谱法。方法：色谱柱为Elite-ODSC18柱（5μm,250mm×4.6mm）,以乙腈-0.1%磷酸二氢钾溶液（10：90）为流动相,流速为1.0mL/min,柱温为室温,检测波长为305nm。结果：葛根素在（0.28-1.96）μg范围内呈线性关系;平均回收率分别为99.3%,RSD为0.94%（n=6）。结论：本法可测定丹葛颈舒胶囊中葛根素的含量,方法简便、准确。     

梯度洗脱法测定心可舒颗粒中葛根素的含量

目的探讨心可舒颗粒中葛根素含量测定的方法。方法采用反相高效液相色谱法,Alltima-C18色谱柱（5μm,4.6×150 mm）;以甲醇-0.2%磷酸二氢钾溶液为流动相进行梯度洗脱,流速为1.0 mL/min;检测波长250nm;柱温25℃。结果葛根素在6.38-63.8μg/mL的范围内与峰面积呈良好的线性关系（r=0.9997）,平均回收率为97.33%,RSD为1.59%（n=5）。结论该方法简便、准确,重现性好,可更好的控制心可舒颗粒的质量。     

RP-HPLC测定心可舒片中葛根素含量

目的:建立心可舒中葛根素的PR-HPLC测定方法.方法:采用RP-HPLC测定心可舒片中葛根素的含量;固定相1∶1,ODS-C18(5 μm);流动相1∶1,甲醇-18.0 ml/L醋酸溶液(20∶80);检测波长:250 nm.结果:平均回收率为100.15%,RSD为1.60%.结论:本法简便、快速,具有实用性.     

HPLC测定消渴胶囊中葛根素的含量

目的:建立消渴胶囊中葛根素的含量测定方法.方法:高效液相色谱法,采用Venusil MP C18柱为分析柱,甲醇-水(25:75)为流动相,检测波长250 nm,流速1.0 mL·min-1.结果:葛根素在0.192-1.536 μg与峰面积呈良好的线性关系(Y=701200X+11824,R2=0.999 9,n=5),平均回收率为99.53%,RSD为1.20%.结论:该方法简便、结果准确、重复性好、专属性好,适用于消渴胶囊中葛根素定量分析.     

脑脉通有效部位中葛根素的含量测定

目的建立脑脉通有效部位中葛根素的含量测定方法。方法采用大连依利特Hypersil ODS2C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-水(30∶70),流速:1.0mL/min,检测波长:250nm。结果葛根素平均回收率为99.72%,RSD=1.49%。结论所建立的方法简便、准确,可作为有效部位的质量控制方法。     

HPLC测定飞脂神来膏喷雾剂中葛根素的含量

目的:建立飞脂神来膏中葛根素含量测定的方法。方法:采用高效液相色谱法,色谱柱为AgilentC-18(4.6mm×250mm,5μm),流动相为乙腈-0.05%磷酸溶液(11:89),流速为1.0mL·min-1,测定波长为250nm,柱温为25℃。结果:葛根素在0.092~0.322ug范围内线性关系良好,平均回收率为100.18%,RSD为1.28%。结论:该方法简便、快捷、稳定,可用于飞脂神来膏的质量控制。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.