Behavioural and biochemical effects of fractions prepared from Banxia Houpu decoction in depression models in mice

Banxia-houpu decoction is widely used in therapy for depression in China. The water-EtOH extract (WE-1) and chloroform-soluble fraction (WE-1-2) of Banxia-houpu decoction reduced the duration of immobility in the tail suspension test (TST) and the forced swimming test (FST), the efficacy of the latter was higher than that of the former. The petroleum-soluble fraction (WE-1-1), ethyl acetate-soluble fraction (WE-1-3) and water-soluble fraction (WE-1-4) tended to possess antiimmobility effects in the TST. After a 21-day treatment, the potency of WE-1-2 was almost equivalent to that of fluoxetine, a selective serotonin reuptake inhibitor (SSRI), in the TST, but WE-1-2 showed a lower efficiency than fluoxetine in the FST. WE-1, as well as WE-1-3, was found to increase serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) levels in mouse hippocampus and striatum, the activities of WE-1 and WE-1-3 were much stronger than that of fluoxetine. Moreover, WE-1-1, WE-1-2 and WE-1-4 significantly elevated 5-HT and 5-HIAA levels only in the striatum, the degrees of increase of 5-HT and 5-HIAA levels were comparable to that of fluoxetine, which significantly enhanced the levels in all regions of animal brains. Neither fractions or fluoxetine affected norepinephrine (NE) and dopamine (DA) levels in the animal brains. It was found that WE-1, WE-1-2 and fluoxetine significantly decreased serum and liver malondialdehyde (MDA) levels in the study. These results provide evidence for a potential role of antidepressive activities in the therapeutic effects of the Banxia-houpu decoction.     

Effect of Bacopa monniera on stress induced changes in plasma corticosterone and brain monoamines in rats

Bacopa monniera (BM) is well known for its neuropharmacological effects. Our previous studies indicated the adaptogenic effect of standardized extract of BM in various stress models. In the present study, effect of BM was evaluated on acute stress (AS) and chronic unpredictable stress (CUS) induced changes in plasma corticosterone and monoamines-noradrenaline (NA), dopamine (DA) and serotonin (5-HT) in cortex and hippocampus regions of brain in rats. Panax root powder (Panax quinquefolium) was taken as standard. Subjecting animals to AS (immobilization for 150 min once only) and CUS (different stressors for 7 days) resulted in significant elevation in plasma corticosterone levels, which was significantly countered by treatment with BM at a dose of 40 and 80 mg/kg p.o. similar to the effects of Panax quinquefolium (PQ) at 100 mg/kg p.o. AS exposure significantly increased the levels of 5-HT and decreased NA content in both the brain regions while DA content was significantly increased in cortex and decreased in hippocampus regions. In CUS regimen, levels of NA, DA and 5-HT were significantly depleted in cortex and hippocampus regions of brain. Treatment with BM (40 and 80 mg/kg) attenuated the stress induced changes in levels of 5-HT and DA in cortex and hippocampus regions but was ineffective in normalizing the NA levels in AS model, whereas PQ treatment significantly reverted back the effects of stress. In CUS model, pretreatment with BM and PQ significantly elevated the levels of NA, DA and 5-HT levels in cortex and levels of NA and 5-HT in hippocampus regions. Hence, our study indicates that the adaptogenic activity of BM might be due to the normalization of stress induced alteration in plasma corticosterone and levels of monoamines like NA, 5-HT and DA in cortex and hippocampus regions of the brain, which are more vulnerable to stressful conditions analogous to the effects of PQ.     

Anti‐depressant Effects of Aqueous Extract from Acanthopanax senticosus in Mice

In this paper, the anti‐depressant effects of Acanthopanax senticosus extract (ASE) were studied using animal models of depression including the forced swimming and tail suspension tests. The anti‐depressive mechanism of ASE was explored by monitoring the levels of monoamine neurotransmitters including 5‐hydroxytrylamine (5‐HT), norepinephrine (NE), and dopamine (DA), as well as cAMP response element‐binding (CREB) protein expression in the whole brain of mice following the tail suspension test. Our results showed that intragastric administration of ASE at a dose of 2000 mg/kg for seven days significantly reduced the duration of immobility in both the forced swimming test and the tail suspension test. These results indicate that ASE possesses antidepressant‐like properties. Pre‐treatment with 2000 mg/kg of ASE for seven days significantly elevated the levels of 5‐HT, NE, and DA in the whole brain of mice. Moreover, ASE at doses of 1000 and 2000 mg/kg significantly up‐regulated the level of CREB protein. Taken together, these findings suggest that the anti‐depressive mechanism of ASE may be mediated via the central monoaminergic neurotransmitter system and CREB protein expression. Therefore, administration of ASE may be beneficial for patients with depressive disorders. Copyright © 2013 John Wiley & Sons, Ltd.     

Antidepressant-like effect of ethanol extract from Zuojin Pill,containing two herbal drugs of Rhizoma Coptidis and Fructus Evodiae,is explained by modulating the monoaminergic neurotransmitter system in mice

Ethnopharmacological relevance

Zuojin Pill (ZJP), a traditional Chinese medicinal decoction, contains two herbal drugs: Coptis chinensis Franch. and Evodia rutaecarpa (Juss.) Benth. in the ratio of 6:1 (w/w). Previous pharmacological studies have shown that two herbs in ZJP have the antagonistic effects on catecholamine secretion in bovine adrenal medullary cells. Furthermore, the alkaloids from the two herbs in ZJP may provide a protective effect for depression in individuals with a low expressing 5-HTT allele by increasing receptor concentration in serotonergic neurons. However, antidepressant effect has not been reported before and has not been fully clarified.

Aim of the study

The present study aimed to investigate the antidepressant potential of ethanol extract from ZJP and its monoaminergic mechanism in mice.

Materials and methods

Seven alkaloids were determined from the ethanol extract of ZJP using High Performance Liquid Chromatography (HPLC) with the gradient mobile phase. The ethanol extract from ZJP was used to evaluate the antidepressant potential in mice. Mouse models of depression including the tail suspension test (TST) and the forced swim test (FST) were used to evaluate the effects of the ethanol extract from ZJP. A possible mechanism was explored in the tests of antagonism of reserpine-induced ptosis and hypothermia, and 5-HTP induced head twitch response in mice. The contents of monoamine neurotransmitters including norepinephrine (NE), serotonin (5-hydroxytryptamine or 5-HT) in hippocampus of mice and NE, 5-HT, dopamine (DA) in striatum of mice were determined by HPLC system with Electrochemical Detector (ECD).

Results

The results showed that intragastric administration of the ethanol extract from ZJP (5, 10, 20 mg/kg) or fluoxetine (7.5 mg/kg) significantly reduced the duration of immobility in TST and FST. However, the effect was not dose-dependent. Ethanol extract from ZJP (5, 10, 20 mg/kg) also increased the accumulative number of the 5-HTP-induced head twitch response in mice. The mice were treated with the ethanol extract from ZJP (5, 10, 20 mg/kg) or fluoxetine (7.5 mg/kg), which could antagonize reserpine-induced ptosis and hypothermia, moreover, both of them could elevate the contents of NE, 5-HT in hippocampus as well as NE, 5-HT, DA in striatum significantly.

Conclusion

These results indicate that the ethanol extract from ZJP produced antidepressant-like effect and the possible mechanism, at least in part, is via the central monoaminergic neurotransmitter system and 5-HT plays a major role.     

达维胶囊对精神分裂症阴性症状小鼠的治疗作用及机理研究

目的：研究达维胶囊（达维）对精神分裂症阴性症状模型小鼠的治疗作用及相关机理。方法：注射低于麻醉剂量的氯胺酮（30mg／kg），制作精神分裂症阴性症状动物模型，用强迫游泳法测定各组小鼠在游泳时保持不动时间，评价达维胶囊对精神分裂症阴性症状小鼠的治疗效果。并用荧光分光光度法测定小鼠全脑内及血清中神经递质（DA，NA，5-HT）含量，探讨其作用机制。结果：与模型组相比，达维大、中剂量组及氯氮平组能使小鼠强迫游泳保持不动时间明显缩短，脑内及血中5-HT含量明显降低，血中NE水平明显升高。结论：达维胶囊对精神分裂症的阴性症状小鼠有一定的治疗作用，其作用机理可能与其能调节脑内5-HT和NE能神经系统功能有关。     

导痰汤合菖蒲郁金汤对卒中后抑郁症的实验研究

目的观察导痰汤合菖蒲郁金汤对卒中后抑郁症(PSD)大鼠模型行为学能力及脑内神经递质含量的影响。方法将80只大鼠随机分为4组,即模型组、导痰汤合菖蒲郁金汤组(中药组)、氟西汀组及假手术组,每组20只。模型组、中药组、氟西汀组采用双侧颈总动脉永久性结扎后行为限制法制备大鼠PSD模型,假手术组仅做颈正中切口即缝合。造模成功后第2d模型组、假手术组均予0.9%氯化钠注射液灌胃,中药组予导痰汤合菖蒲郁金汤灌胃,氟西汀组予盐酸氟西汀水溶液灌胃,均连续给药28d。测定大鼠海马区5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)、5-羟基吲哚乙酸(5-HIAA)含量的变化,并进行糖水消耗试验。结果假手术组、中药组及氟西汀组糖水消耗量均高于模型组(P0.05),纯水消耗量均低于模型组(P0.05);假手术组、中药组及氟西汀组NE、DA、5-HT及5-HIAA含量均高于模型组(P0.05);中药组NE、5-HT及5-HIAA含量均低于假手术组(P0.05);氟西汀组NE、DA含量均低于假手术组(P0.05)。结论导痰汤合菖蒲郁金汤可以改善PSD大鼠的行为及脑内单胺类神经递质的含量。     

Chinese medicine Banxia-houpu decoction regulates c-fos expression in the brain regions in chronic mild stress model in rats

Banxia-houpu decoction is a safe and effective traditional Chinese medicinal formula used in the treatment of mild and manic-depressive disorders for centuries. There has been increasing interest in its therapeutic application in depression. However, the mechanisms behind behavioural changes are still poorly understood. Chronic mild stress (CMS)-induced preference behaviour change has been used as a model to predict the clinical efficacy of many types of antidepressant treatment. Both EtOH and water extracts (AE and WE) of Banxia-houpu decoction exhibited a significantly increased sucrose intake in the CMS model in rats, but there was no effect in unstressed animals. In the present study, it was found that the c-fos expression in cerebral cortex, hippocampus and striatum corpora were very high in the CMS model in rats. WE and AE at a dose of 130 mg/kg exhibited a significantly decreased c-fos expression in the cerebral regions in CMS model in rats, respectively. The former was more potent than the latter. However, no significant changes in the c-fos expression were observed in unstressed rats treated with the decoction. Fluoxetine not only significantly reduced c-fos expression in all regions in the CMS model in rats, but only showed a marked decrease in c-fos expression in the hippocampus in unstressed animals. A different molecular mechanism of Banxia-houpu decoction and fluoxetine may be implied. The cerebral cortex, hippocampus and striatum conpora might be important structural substrates in the central nervous system mediating the section of the Banxia-houpu decoction on preference behaviour in CMS-induced rats, and fos protein might be the common substrate of the signal transduction process of the decoction.     

Involvement of monoamines and proinflammatory cytokines in mediating the anti-stress effects of Panax quinquefolium

Panax quinquefolium (PQ) is well acclaimed in literature for its effects on central and peripheral nervous system. The present study explores the effects of PQ on stress induced changes of corticosterone level in plasma, monoamines (NA, DA and 5-HT) and interleukin (IL-2 and IL-6) levels in cortex and hippocampus regions of brain and also indicate their possible roles in modulating stress. Mice subjected to chronic unpredictable stress (CUS, for 7 days) showed significant increase in plasma corticosterone level and depletion of noradrenaline (NA), dopamine (DA) and 5-hydroxytryptamine (5-HT) levels in cortex and hippocampal regions along with an increased level of IL-2 and IL-6 in the same areas. Aqueous suspension of PQ was administered daily at a dose of 100 and 200 mg/kg p.o. prior to the stress regimen and its effects on selected stress markers in plasma and brain was evaluated. PQ at a dose of 200 mg/kg p.o. was found to be effective in normalizing the CUS induced elevation of plasma corticosterone and IL-2, IL-6 levels in brain. Moreover, it was significantly effective in reinstating the CUS induced depletion of NA, DA and 5-HT in hippocampus, while NA and 5-HT in cortex of brain. However, PQ at a dose of 100 mg/kg p.o. was found ineffective in regulating any of these CUS induced changes. Present study provides an insight into the possible role of PQ on hyperactive HPA axis in the regulation of immediate stress effectors like corticosterone, cytokines and brain monoamines. In this study, PQ has emerged as a potential therapeutic in the cure of stress related disorders and needs to be evaluated in clinical studies to ascertain its efficacy.     

Effects of Fuhe decoction on behaviors and monoamine neurotransmitters in different brain regions of CUMS combined with social isolation depression model rats

ObjectiveTo investigate the effect of Fuhe decoction on the behavior and levels of monoamine neurotransmitters in different brain regions in a depression rat model induced by chronic unpredictable mild stimulation (CUMS) combined with social isolation.MethodsFifty male SD rats were randomly divided into a blank group, model group, fluoxetine group, Chaiqinwendan decoction group, and Fuhe decoction group. Chronic unpredictable mild stimulation combined with a social isolation method was used to replicate the depression rat model. After 42 days of administration, a tail suspension test and high-performance liquid electrochemical detection (HPLC-ECD) were used to detect the behavioral changes and changes in the content of monoamine neurotransmitters norepinephrine (NE), dopamine (DA), 5-hydroxytrytamine (5-HT), and metabolites in different brain regions of rats in each group before and after treatment.ResultsCompared with the model group, the epinephrine (E) content in the Fuhe decoction group was highly significantly increased (P < .01). Compared with the model group, the 5-HT content of the prefrontal cortex in rats in the Fuhe decoction group was highly significantly increased (P < .01). Furthermore, compared with the model group, the 5-HT content in the hippocampus of rats in the Fuhe decoction group was significantly increased (P < .05).ConclusionFuhe decoction can improve the depression-like behaviors of model rats, and its antidepressant effect may be related to the increase in 5-HT content in the prefrontal cortex and hippocampus of rats.     

Pharmacological study on antidepressant activity of 50% ethanol extract of a formulated ayurvedic product in rats

The effects of 50% ethanol extract of one formulated ayurvedic product, consisting of a mixture of medicinal plant species, was investigated on behavioral despair test (forced swimming test, FST), central dopaminergic and serotonergic activity in rats. The effects on the forced swimming test were assessed along with the levels of dopamine (DA), serotonin (5-HT) and its metabolites homovanillic acid (HVA) and 5-hydroxyindoleaceticacid (5-HIAA) in striatum, frontal cortex, hippocampus, hypothalamus and brain stem after 21 days of chronic oral administration of the extract (500 and 1500 mg/kg-body weight). The extract significantly increased climbing behavior at 500 mg/kg and increased swimming behavior by reducing immobility time at 1500 mg/kg when compared with the control group in forced swimming test (P<0.05). This showed that the active substances present in 50% ethanol extract of the ayurvedic preparation possess antidepressant activity and their specificity towards particular behavior, depends on the concentration of the extract. Further it showed that the enhancement of active behavior in FST is not due to generalized motor activity. The neurochemical estimations revealed the swim stressor inducing alterations in the levels of DA, 5-HT and their metabolites HVA and 5-HIAA in the brain regions assayed as compared with the non-stressed control rats. These changes were prevented extract treated rats. The 500 mg/kg extract treated group had significantly increased the levels of DA in frontal cortex, hypothalamus and hippocampus whereas the 5-HT in hypothalamus (P<0.05). However, there were no significant changes in the levels of HVA and 5-HIAA. These behavioral and biochemical results indicate antidepressant properties of the extract, which may be mediated by the dopaminergic and serotonergic mechanisms in rat brain.     

Effect of Acanthopanax senticosus Harms on biogenic monoamine levels in the rat brain

The extract of the stem bark of Acanthopanax senticosus Harms (ASH) is known to have healing and protective effects on stress-induced disturbance of mental status. We have analysed whether a single or chronic (2 week) administration of ASH can affect concentrations of noradrenaline (NA), dopamine (DA) and their metabolites in the normal rat brain. A single p.o. administration of ASH elevated the NA and DA levels in the whole brain of rats in a dose-dependent manner. A single or 2 week administration of ASH (500 mg/kg) showed a marked increase in the DA level only in the striatum. However, NA levels were increased by a single dose of ASH in a wide range of brain regions such as the cortex, hypothalamus, striatum, hippocampus, substantia nigra and pons. When administered for 2 weeks no increase in NA levels was seen in these brain regions, except for an increase in the frontal cortex and anterior hypothalamus. The present results suggest that ASH may act by regulating NA and DA levels in specific brain regions related to stress response and Parkinson's disease.     

开心解郁汤对抑郁模型大鼠脑内单胺类神经递质的影响

目的:观察开心解郁汤对抑郁模型大鼠脑内杏仁核去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)含量的影响。方法:将30只SD雄性大鼠随机分为正常组、模型组、给药组,每组10只;用慢性应激结合孤养的方式造模21 d。在造模同时,给药组ig开心解郁汤40 g.kg-1,1次/d,连续21 d。用高效液相-电化学方法检测大鼠脑内NE,DA,5-HT含量的变化。结果:与正常组比较,模型组大鼠脑内NE,DA,5-HT含量明显减少;与模型组比较,开心解郁汤可显著升高抑郁模型大鼠脑内NE,DA,5-HT的含量(P<0.05)。结论:开心解郁汤的抗抑郁作用机制可能与调节脑内单胺类神经递质的平衡有关。     

半夏厚朴汤及其组成中药抗焦虑和抑郁研究进展

目的：综述半夏厚朴汤及其组成中药抗焦虑和抑郁研究进展。方法：查阅国内外近年来有关文献结合相关的研究成果，从半夏厚朴汤对动物焦虑和抑郁行为、神经生化机制、免疫系统功能、血脂代谢、机体氧化防御系统等层次进行阐述。结果与结论：半夏厚朴汤及其组成中药抗焦虑和抑郁具有一定的科学性。     

柴胡疏肝散对抑郁症大鼠海马神经递质含量的影响

目的探讨柴胡疏肝散对抑郁症大鼠海马神经递质含量的影响。方法将60只sD大鼠随机分为空白组、模型组、氟西汀组、柴胡疏肝散中剂量组和高剂量组,每组12只。除空白组外其余4组大鼠连续14天腹腔注射利血平注射液造模。造模后氟西汀组和柴胡疏肝散中、高剂量组每天分别给予9mL／kg氟西汀、5g／kg和10g／kg柴胡疏肝散灌胃。给药开始后第0、4、7、10、14天测量体重,第0、7、14天计算Open—Field行为得分,第15天处死动物,采用ELISA法测定海马组织单胺类神经递质5-羟色胺（5-HT）、去甲。肾上腺素（NE）、多巴胺（DA）的含量。结果与模型组比较,柴胡疏肝散高剂量组体重,水平活动和垂直活动得分,海马单胺类神经递质5-HT、NE和DA含量均显著增加,差异均有统计学意义（P〈0．05或P〈0．01）;柴胡疏肝散中剂量组水平活动得分,5-HT和DA含量均显著增加,差异均有统计学意义（P〈0．05或P〈0．01）。结论中药柴胡疏肝散抗抑郁效果显著,可能与增加海马组织单胺类神经递质含量有关。     

Antidepressant-like effect of ethanol extract from Paeonia lactiflora in mice

The present study investigated the antidepressant effect of ethanol extract of Paeonia lactiflora (EPL) in mice using forced swim test, tail suspension test, open-field test and reserpine test. Our results showed that intragastric administration of EPL at the doses of 250 and 500 mg/kg for seven days significantly reduced the duration of immobility in both forced swim test and tail suspension test. EPL at the dose of 500 mg/kg was as effective as the positive control (chlorimipramine, 20 mg/kg) in these tests. However, these treatments did not affect the number of crossing and rearing in the open-field test. Treating mice with EPL at the doses of 250 and 500 mg/kg significantly antagonized reserpine-induced ptosis and hypothermia. However, at the dose of 125 mg/kg, EPL antagonized only the hypothermia but not ptosis induced by reserpine. The results clearly demonstrated the antidepressant effect of Paeonia lactiflora in animal models of depression. The action of Paeonia lactiflora may be mediated via the central monoaminergic neurotransmitter system.     

Hange-koboku-to, a Kampo medicine, modulates cerebral levels of 5-HT (5-hydroxytryptamine), NA (noradrenaline) and DA (dopamine) in mice

Cerebral monoamine systems play important pathogenic roles in various psychiatric and neurologic diseases, such as depression, anxiety and swallowing disturbance. Hange-koboku-to, a Kampo (Japanese herbal) medicine, has been successfully used for the treatment of these disorders. To elucidate the mechanisms underlying its clinical efficacy for these disorders, the effects of Hange-koboku-to (500 mg/kg, p.o.) on the cerebral monoamine systems were examined. Regional levels of 5-HT (5-hydroxytryptamine), NA (noradrenaline), DA (dopamine) and their metabolites in mouse brain were measured using a high-performance liquid chromatography system. Hange-koboku-to increased the 5-HT and NA levels and decreased 5-HIAA (5-hydroxyindole-3-acetic acid), thus decreasing 5-HT and NA turnover (metabolites/monoamine ratio) in the hypothalamus. The levels of DA, DOPAC (3,4-dihydroxyphenylacetic acid) and HVA (4-hydroxy-3-methoxy-phenylacetic acid) were all increased, resulting in a decreased DA turnover in the striatum. Since decreased 5-HT turnover has been observed after administration of various antidepressants, Hange-koboku-to-mediated reduction of 5-HT turnover may be related to the clinical efficacy of this Kampo medicine on certain psychiatric disorders. Furthermore, the beneficial therapeutic effects of Hange-koboku-to on swallowing disturbance may be related to the increased cerebral DA level brought about by this Kampo medicine.     

何首乌饮对雌性衰老大鼠下丘脑神经递质的影响

目的 探讨下丘脑衰老时神经递质的变化及何首乌饮延缓下丘脑衰老的作用机制.方法 D-半乳糖连续腹腔注射致亚急性衰老动物模型,造模后灌胃何首乌饮连续60 d后,大鼠断头取下丘脑.采用ELISA法检测各组大鼠SP,β-EP和GnRH的含量;高效液相色谱法(HPLC)以SHIMADZU-10A 高效液相色谱仪和L-ECD-6A电化学检测器检测下丘脑单胺类神经递质NA,DA,5-HT的含量.结果 同正常对照组相比,模型组大鼠下丘脑GnRH、SP明显增高,β-EP与NA、DA、5-HT含量明显降低,差异有统计学意义(P<0.05).与模型组比较,何首乌饮组下丘脑GnRH、SP明显下降,β-EP与NA、DA、5-HT含量显著升高,呈现一定的剂量依赖性.结论 衰老时大鼠下丘脑正常功能被破坏,何首乌饮通过对神经递质紊乱的调节而延缓了下丘脑的衰老.     

调补气血针法对SAMP10脑中枢神经递质含量影响的研究

目的 :旨在揭示调补气血针法治疗痴呆的中枢作用机制。方法 :采用HPLC -ECD法对SAMP10及SAMR1脑组织单胺类神经递质和乙酰胆碱 (Ach)含量进行测定。结果 :①SAMP10脑组织DA、5 -HT、Ach的含量显著低于SAMR1的含量 ;②调补气血针法可使脑组织降低的DA、5 -HT、Ach的含量显著提高。结论 :①SAMP10小鼠的学习、记忆障碍发病机制之一可能是脑组织Ach和单胺类神经递质的代谢异常出现了神经损害 ;②调补气血针法改善了SAMP10的学习、记忆状况     

参竹精片协同戊巴比妥钠对小鼠睡眠及5-HT的影响

[目的]观察参竹精片对睡眠的影响。[方法]选昆明小鼠随机分为正常对照组、参竹精片0.5、0.25、0.125 g/kg组,分别单次灌胃给药和连续7 d给药,末次给药后2 h,各组小鼠分别以30 mg/kg(阈下剂量)和50 mg/kg(阈剂量)腹腔注射戊巴比妥钠,观察小鼠入睡时间、入睡率及睡眠时间,测定脑干组织5-羟色胺(5-HT)、5-羟基吲哚乙酸(5-HIAA)含量。[结果]与正常组比较,单次灌胃给药和连续7 d给药,参竹精片可明显增加戊巴比妥钠(阈下剂量)致小鼠的入睡率(0.5 g/kg,P0.05),减少戊巴比妥钠(阈剂量)致小鼠的入睡时间(P0.01),延长睡眠时间(P0.01),增加脑干组织5-HT含量(P0.05)。[结论]参竹精片可促进脑组织分泌5-HT,减少入睡时间、提高入睡率、延长睡眠时间,具有一定改善睡眠的作用。