共查询到20条相似文献,搜索用时 78 毫秒
1.
目的 研究增龄对离体大鼠主动脉内皮依赖性舒张的影响.方法 成年及老年大鼠各20只,利用组织器官水浴系统,通过乙酰胆碱(Ach)、N-硝基左旋精氨酸,测量离体主动脉环的舒张反应变化(%).结果 1)老年组大鼠主动脉环对10-8~10-4mol/L Ach诱发的内皮依赖性血管舒张反应均较成年组明显减弱,两组之间差异有非常显著性意义(P< 0.01).2)离体大鼠主动脉环经10-4mol/L L-NNA预处理20分钟,阻断一氧化氮合酶(NOS)后,同样剂量的Ach不再诱发上述内皮依赖性舒血管反应.结论 随增龄血管内皮分泌的一氧化氮(NO)逐渐减少,导致血管内皮依赖性舒张减弱;NOS的异常表达对NO 减少起着重要的作用,eNOS随增龄表达减弱,而iNOS 随增龄表达则明显增强. 相似文献
2.
目的观察异丙肾上腺素(Iso)、乙酰胆碱(Ach)、氨茶碱(Ami)对不同年龄、性别大鼠主动脉血管反应性的影响。方法采用离体血管张力实验方法,观察Iso,Ach,Ami对20mmol/LKCl引起的1,3,6,12月龄大鼠胸主动脉收缩的影响。结果Iso(10-9-10-5mol/L)对1月龄大鼠主动脉的舒张在雄性、雌性大鼠分别为(44.02±7.89)%和(63.83±9.50)%,随年龄的增加舒张作用减弱,甚至有收缩作用。Ach(10-8-3×10-5mol/L)对1月龄大鼠主动脉舒张作用最大,在雄性、雌性分别达到(68.17±7.02)%和(81.24±4.64)%,其余各年龄组舒张作用明显减少。Ami(10-5-3×10-4mol/L)对1月龄大鼠主动脉舒张在雄性、雌性分别达到(83.25±4.34)%和(82.83±7.71)%,其舒张作用随年龄的增加而减弱。结论Iso,Ach,Ami对大鼠主动脉环的舒张作用在雌性明显,并随着年龄的增加而降低。 相似文献
3.
目的:探讨槲皮素(Que)对大鼠离体胸主动脉环的舒张作用及其舒张机制.方法:采用离体血管环技术制备大鼠胸主动脉环,使用累积加药法,检测Que对去甲肾上腺素预收缩的胸主动脉环张力的影响.结果:Que对离体大鼠内皮完整和去内皮的胸主动脉环均呈剂量依赖性扩血管作用.在小剂量Que(1×10-6,3×10-6,1×10-5mol/L)浓度,内皮完整和去内皮胸主动脉环的扩血管作用之间差异无显著统计学意义,但在较大剂量Que (3×10-5,6×10-5mol/L),去内皮后其舒血管作用显著性减弱(P<0.05).用环氧合酶抑制剂吲哚美辛,钙激活钾通道抑制剂CHTX分别预处理后,Que在各剂量组引起的舒张血管作用与不加阻滞剂比较无统计学意义(P>0.05).用一氧化氮合酶抑制剂L-NAME预处理后,Que在较大剂量(3×10-5,6×10-5mol/L)引起的舒张血管作用明显减低,与不加阻滞剂比较,有统计学差异(P<0.05).结论:槲皮素具有剂量依赖性的舒张血管作用.其舒张作用与前列腺素、钙激活钾通道无关,而与内皮一氧化氮有关. 相似文献
4.
西红花酸部分内皮依赖性血管舒张作用及其机制 总被引:1,自引:0,他引:1
目的:研究西红花酸(crocetin,CCT)血管舒张作用及其可能机制.方法:采用RM6240C型多道生物信号采集处理系统,记录灌流大鼠胸主动脉环张力变化.结果:CCT(10-9 ~10-4mol/L)对苯肾上腺素(PE,10-5 mol/L)预收缩的内皮完整血管环产生浓度依赖性的舒张作用;CCT(3×10-9~10-4mol/L)对苯肾上腺素(PE,10-5 mol/L)预收缩的去内皮血管环产生浓度依赖性的舒张作用,但内皮完整组血管舒张作用比去内皮组更明显,两者相比,在10-8~10-4mol/L时,具有显著性差异(P<0.01).CCT对高浓度KCl(6×10-2mol/L)预收缩的血管环无明显的舒张作用.一氧化氮合酶抑制剂左旋硝基精氨酸甲酯(10-4 mol/L)或前列环素合成酶抑制剂吲哚美辛(10-5 mol/L)预处理,对CCT诱导的内皮完整动脉环舒张作用具有明显的抑制效应.ATP敏感性K+通道阻断剂格列苯脲(3×10-6 mol/L )预处理后,CCT的舒血管作用均无显著变化(P >0.05),但Ca2+ 激活K+通道抑制剂四乙胺(5×10-3 mol/L )或电压敏感性K+通道抑制剂4-氨基吡啶(10-3 mol/L)预处理,可明显减弱CCT引起的无内皮血管舒张作用(P<0.01).结论:CCT对大鼠胸主动脉具有部分内皮依赖性舒张作用,其内皮依赖性血管舒张作用的机制,与其促进内皮一氧化氮合酶或环氧合酶途径,从而增加一氧化氮和前列环素的生成有关;其内皮非依赖性舒血管效应的作用机制涉及Ca2+激活K+通道或电压敏感型K+通道的激活. 相似文献
5.
目的 观察磷酸二酯酶(PDE)1和PDE3亚型在大鼠胸主动脉中的表达及其对血管张力的调节作用.方法 采用RT-PCR检测PDE1和PDE3亚型mRNA的表达.将大鼠胸主动脉制备成2~3 mm的血管环,苯肾上腺素(PE)诱导血管环收缩后,分别用特异性PDE1抑制剂IC86340和PDE3抑制剂milrinone处理去除内皮组和保留内皮组的血管环,测定两组舒张血管的作用.结果 ①PDE1A、PDE1B、PDE1C、PDE3A和PDE3B亚型mRNA在大鼠胸主动脉均有表达.②在保留内皮组和去除内皮组,IC86340均舒张PE诱发的血管收缩(P<0.01);并且对去除内皮组的舒张作用小于保留内皮组(P<0.01).③在保留内皮和去除内皮组,milrinone均可舒张PE诱发的血管收缩(P<0.01);但milrinone在低浓度时(1×10-6.5~1×10-6 mol/L),去除内皮组舒张血管的作用大于保留内皮组(P<0.05),而高浓度(1×10-5.5~1×10-5 mol/L)时两组差异无统计学意义(P>0.05).结论 PDE1和PDE3亚型mRNA在大鼠胸主动脉表达并参与血管张力的调节.PDE1部分通过血管内皮依赖的机制调节血管张力,PDE3则通过内皮非依赖的途径调节血管张力. 相似文献
6.
目的 :通过观察血红素氧化酶抑制剂锌原卟啉Ⅸ (zineprotoporphyrinⅨ ,ZnPPⅨ )对乙酰胆碱 (acetyl choline,Ach)内皮依赖性血管舒张反应的影响 ,探讨内源性一氧化碳 (carbonmonoxide,CO)对肺血管的舒张作用。方法 :取Wistar大鼠肺动脉环 ,制备Ach浓度效应曲线 ,冲洗后平衡 6 0min ,分别加入一氧化氮 (nitricoxide,NO)合成酶抑制剂L NAME 30 μmol/L或L NAME 30 μmol /L +ZnPPⅨ 10 μmol/L ,孵育 30min后 ,重复制备Ach浓度效应曲线 ,观察给药前后Ach浓度效应关系的变化。结果 :用去氧肾上腺素收缩肺动脉后 ,Ach能对内皮完整的肺动脉引起浓度依赖性舒张 ,血管环用L NAME孵育后 ,舒张反应受抑 ,10 -5mol/L的Ach只引起 (40 .3± 5 .1) %的舒张反应 ,而用ZnPPⅨ及L NAME共同孵育后 ,舒张反应进一步受抑或消失 ,10 -5mol/L的Ach只能引起 (11.1± 8.4 ) %的舒张反应或不引起舒张反应。结论 :ZnPPⅨ可抑制Ach的内皮依赖性舒张反应 ,提示内源性CO与NO共同参与了Ach的内皮依赖性血管舒张反应 ,CO对肺血管平滑肌有舒张作用 相似文献
7.
目的: 通过观察血红素氧化酶抑制剂锌原卟啉Ⅸ(zine protoporphyrin Ⅸ,ZnPP Ⅸ)对乙酰胆碱(acetylcholine, Ach)内皮依赖性血管舒张反应的影响,探讨内源性一氧化碳(carbon monoxide, CO)对肺血管的舒张作用.方法:取Wistar大鼠肺动脉环,制备Ach浓度效应曲线,冲洗后平衡60 min,分别加入一氧化氮(nitric oxide, NO)合成酶抑制剂L-NAME 30 μmol/L或L-NAME 30 μmol /L+ZnPP Ⅸ 10 μmol/L,孵育30 min后,重复制备Ach浓度效应曲线,观察给药前后Ach浓度效应关系的变化.结果:用去氧肾上腺素收缩肺动脉后,Ach能对内皮完整的肺动脉引起浓度依赖性舒张,血管环用L-NAME孵育后,舒张反应受抑,10-5mol/L的Ach只引起(40.3±5.1)%的舒张反应,而用ZnPP Ⅸ及L-NAME共同孵育后,舒张反应进一步受抑或消失,10-5mol/L的Ach只能引起(11.1±8.4)%的舒张反应或不引起舒张反应.结论:ZnPP Ⅸ可抑制Ach的内皮依赖性舒张反应,提示内源性CO与NO共同参与了Ach的内皮依赖性血管舒张反应,CO对肺血管平滑肌有舒张作用. 相似文献
8.
三七总皂甙对低氧高二氧化碳性肺血管收缩的影响 总被引:1,自引:0,他引:1
目的:探讨三七总皂甙(PNS)对肺动脉平滑肌的舒张作用及其与肺动脉内皮细胞的关系。方法:采用大鼠离体肺主动脉环灌流模型。分别在常氧及低氧高二氧化碳条件下,观察PNS对氯化钾(KCl)及去甲肾上腺素(NE)预收缩内皮完整血管和去内皮血管的舒张作用。结果:①在常氧及低氧高二氧化碳条件下,累积浓度的PNS对60mmol/LKCl及10-5mol/LNE预收缩的血管环均产生剂量依赖性舒张作用(P<0.05)。②低氧高二氧化碳增强了PNS的肺血管舒张作用,与相应浓度各常氧组相比,均有统计学意义(P<0.05或P<0.01)。③常氧条件下,去内皮组与内皮存在组相比,PNS的血管舒张效应无明显差异(P>0.05);但低氧高二氧化碳条件下,去内皮组的PNS血管舒张效应远大于内皮完整组(P<0.05或P<0.01)。结论:PNS可减轻低氧高二氧化碳性肺血管收缩,且去除肺动脉内皮后这种作用更加明显,其机制可能与内皮介导作用的缺失有关。 相似文献
9.
目的 探讨黄芪对过氧亚硝酸盐阴离子(ONOO-)导致的血管反应性异常的影响.方法 32只SD大鼠随机分为2组,正常对照组与黄芪灌胃组,每组16只.大鼠喂养4周后处死,取胸主动脉,制备离体血管环标本.每组大鼠血管随机分为不同处理组(ONOO-组、未处理组)孵育.完成孵育后采用大鼠离体主动脉环灌流模型观察2组大鼠血管对苯肾上素(PE)/乙酰胆碱(Aeh)诱导的收缩/舒张反应性的变化.结果 正常对照组不同处理血管在PE的作用下收缩反应性均呈浓度依赖性增高;在高浓度(10、100 μmol/L)时,ONOO-处理组血管对PE的最大收缩力明显高于未处理组(P均<0.05);2组血管在Ach的作用下舒张反应性均呈浓度依赖性增高,在高浓度(100 μmol/L)时,ONOO-处理组血管对Ach的最大舒张率明显低于未处理组(P<0.05).在黄芪灌胃ONOO-处理组与未处理组大鼠血管上述改变均无明显差异(P>0.05).表明ONOO-导致的血管舒缩功能异常可以被黄芪改善.结论 黄芪可改善ONOO-导致的血管反应性异常,因此对血管功能具有保护作用. 相似文献
10.
黄芪舒张血管平滑肌的作用及机制 总被引:14,自引:0,他引:14
目的:探讨黄芪对血管平滑肌的舒张作用及其机制.方法:采用大鼠离体胸主动脉环灌流模型.在去除血管环内皮后,观察累积浓度黄芪对基础状态(基础组)、苯肾上腺素(PE)预收缩(苯肾上腺素组)、氯化钾预收缩(氯化钾组)的血管环的作用和黄芪对经无钙液(无钙液组)、无钙液加肝素(肝素组)、普萘洛尔(普萘洛尔组)预处理后的血管环的作用.结果:在血管内皮被去除后,黄芪对基础状态或氯化钾预收缩的血管环无影响;当黄芪浓度累积达(10-1、3×10-1、100、3×100)g/L时,对PE(3×10-7mol/L)预收缩的血管环产生剂量依赖性舒张作用(P<0.05).经无钙液预处理后,黄芪(3×100 g/L)对血管环的舒张作用未被阻断;但经无钙液加肝素预处理后,该作用被显著抑制(P<0.01);而普萘洛尔预处理不影响黄芪对PE预收缩血管环的舒张作用.结论:黄芪对去除内皮的血管具有舒张作用.其机制可能与阻断血管平滑肌细胞内质网上的三磷酸肌醇敏感的钙离子通道,抑制内钙的释放有关. 相似文献
11.
Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P < 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (>0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P <0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P < 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD. 相似文献
12.
Jiageng Zhu Jun Li Ruipeng Ji Jianghao Su Mingshun Shen Zhigang Cao 《南京医科大学学报(自然科学版)》2007,21(2):125-128
Objective: To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods: SD rats served as both donors and recipients. 4℃ sodium lactate Ringer's was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava (IVC) inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results: Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion: This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment. 相似文献
13.
Tao Ye * Zhi-quan Liu Jian-jun Mu Xi-han Fu Jun Yang Bao-lin Gao and Xiao-hong Zhang Department of Cardiology First Hospital of Xi'an Jiaotong University Xi'an Hanzhong Cardiovascular Institute Hanzhong 《中国医学科学杂志(英文版)》2004,19(4):248-251
Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P< 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P< 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure. 相似文献
14.
目的:评价使用安心颗粒对急诊经皮冠状动脉介入术(PPCI)术后生活质量的影响.方法:将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组(术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次)和对照组(仅接受基础药物治疗).所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表(MIDAS)、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果:入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异(P>0.05);出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低(P<0.05);组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低(P<0.05).两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血(P>0.05).两组发生轻度血小板减少症的患者数比较无差异(P>0.05).结论:PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险. 相似文献
15.
Jingming Zhu Ningning Hui Xiuhong Jiang Huaxing Zhang Chengzhang Fu 《南京医科大学学报(自然科学版)》2007,21(6):386-389
Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P<0.01);the MAP decreased(P<0.01)and the HR increased drastically(P<0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients. 相似文献
16.
Objective:To investigate the gene expression of osteoprotegerin(OPG) and osteoclast differentiation factor(ODF) in the bone tissue of patients with hip fracture due to osteoporosis. Methods:OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers(over 50 years old) with hip fracture(Observer Group) and 30 cases of hip facture sufferers with no osteoporosis(Control group) were analyzed with the Semi-Quantitative RT-PCR method. Results:The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion:Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption. 相似文献
17.
Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P. 相似文献
18.
Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine. 相似文献
19.
Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center. 相似文献
20.
Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine
下载免费PDF全文
![点击此处可从《中国结合医学杂志》网站下载免费的PDF全文](/ch/ext_images/free.gif)
A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care(~'4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes. 相似文献