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1.
目的 观察凋亡及凋亡调控基因Caspase-3/Bax在增生性玻璃体视网膜病变(PVR)中视网膜前膜(epiretinal membrane)的表达及与凋亡的相关性。方法20例增生性玻璃体视网膜病变的视网膜前膜标本,分别进行Caspase-3和Bax免疫组织化学染色,观察表达情况及染色强度。结果Caspase-3及Bax在视网膜前膜中有较好的表达。正常视网膜组织中未见Caspase-3及Bax的表达。结论在视网膜前膜中存在细胞凋亡,Caspase-3及Bax的表达表示视网膜前膜的细胞凋亡的程度。  相似文献   

2.
Huang W  Wang L  Yuan M  Ma JX  Hui YN 《中华眼科杂志》2004,40(5):317-320
目的 探讨肾上腺髓质素(ADM)及其受体(ADMR)在增生性玻璃体视网膜病变(PVR)前膜中的表达。方法 标本取自2001年7-11月在我院眼科研究所确诊为孔源性视网膜脱离合并PVR行玻璃体手术的10例连续病例,PVR均为C级以上,采用原位杂交法对术中所取标本的ADM和ADMR mRNA表达情况进行观察。结果10例增生性玻璃体视网膜病变前膜中有7例ADM和ADMR mRNA阳性。阳性细胞内分布不均匀,部分细胞以胞质阳性为主,部分细胞的阳性主要出现在胞核。结论 ADM和ADMR在PVR前膜中表达,PVR的发病过程中有ADM和ADMR的参与。(中华眼科杂志,2004,4D:317-320)  相似文献   

3.
目的:研究增生性玻璃体视网膜病变视网膜前膜(epiretinal membrane,ERM)的超微结构及肝细胞生长因子(hepatocyte growth factor,HGF)受体的表达情况。方法:严重增生性玻璃体视网膜病变(PVR)患10例行玻璃体手术时取出视网膜前膜,透射电镜观察ERM的组成成分,免疫组化染色观察ERM组织中HGF。受体的表达。结果:ERM以上皮样细胞、成纤维样细胞为主并含有大量胶原成分。ERM标本中HGF受体集中在细胞分布密集区域表达,阳性细胞多是一些含色素颗粒的细胞。结论:视网膜色素上皮(RPE)细胞是PVR的主要参与细胞,HGF可能参与了PVR膜形成。  相似文献   

4.
目的观察单核细胞趋化蛋白-1(monocyte chemotac ticprotein-1,MCP-1)和基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)在增生性玻璃体视网膜病变(PVR)增生膜中的表达。方法 玻璃体手术取出的PVR增生膜24例,其中C级膜11例,D级膜13例,用免疫组织化学方法检测MCP-1和MMP-2的表达。结果在全部24例增生膜中,MCP-1和MMP-2均有表达,MCP-1阳性表达8例,强阳性表达16例;MMP-2阳性表达7例,强阳性表达17例。结论MCP-1和MMP-2均存在于PVR增生膜中,提示2者在PVR增生膜的形成和PVR发生发展过程中起重要作用。  相似文献   

5.
增生性玻璃体视网膜病变视网膜前膜CD44v6的表达   总被引:2,自引:1,他引:2  
目的:观察增生性玻璃体视网膜病变视网膜前膜中CD44v6的表达情况。方法:用免疫组化方法对13例PVR患行玻璃体切割术的剥离的视网膜前膜进行观察。结果:CD44v6阳性表达10例,表达率77%。结论:提示CD44v6在PVR的形成和发展中有一定的作用。  相似文献   

6.
目的 观察增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)C、D两级视网膜前膜 (epiretinal membranes, ERM)和培养的人视网膜色素上皮(retinal pigment epithelium, RPE)细胞中肝细胞生长因子受体(hepatocyte growth factor receptor, HGFR)的表达情况。 方法 采用免疫组织化学染色方法对15例复杂孔源性视网膜脱离患者玻璃体切割术中剥离的ERM以及培养的人RPE细胞中HGFR的表达情况进行观察。 结果 在6例PVR C级和9例PVR D级ERM标本中分别有5、7例呈HGFR阳性表达;培养的RPE细胞胞浆中HGFR呈阳性表达。 结论 肝细胞生长因子有可能参与了 PVR的病理过程。 (中华眼底病杂志, 2002, 18: 221-223)  相似文献   

7.
目的 观察表皮生长因子受体(epidermalgrowthfactor receptor,EGFR)在增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)增生膜中的表达及其与病程的关系。方法 玻璃体手术中取出的PVR增生膜43例,按病程分为早期膜(<2个月),中期膜(2—6个月)和晚期膜(>6个月),用免疫组织化学及原位杂交技术从蛋白质及mR—NA水平检测EGFR的表达。结果 EGFR蛋白分子在早期膜中呈强阳性表达,中期膜中呈弱表达,晚期膜中呈阴性。EGFR基因仅在早期膜中呈阳性表达。结论 EGFR主要存在于PVR的早期膜中,可能介导有丝分裂信号对PVR的发生发展所起的重要调控作用。  相似文献   

8.
增生性玻璃体视网膜病变中组织型转谷氨酰胺酶的表达   总被引:2,自引:2,他引:0  
目的:检测增生性玻璃体视网膜病变(PVR)视网膜前膜和视网膜色素上皮(RPE)细胞中组织型转谷氨酰胺酶(tTG)的表达情况,探讨tTG是否参与PVR的发病过程.方法:PVR患者21例,收集视网膜前膜,C级8例,D级13例,行tTG免疫组织化学染色.另对培养3-5代的人RPE细胞分别用含有1 00mL/L PVR玻璃体液、正常玻璃体液和PBS的DMEM培养液进行孵育24h后行tTG免疫细胞化学染色.光镜下观察,比较C级和D级膜的表达阳性率,显微图像分析测量3组RPE细胞染色的平均灰度值.结果:在PVR视网膜前膜中tTG呈阳性表达(81%),C级和D级膜表达阳性率无差异 (C级88%,D级77%,P>0.05).培养的人RPE细胞表达tTG,在PVR患者的玻璃体液作用下,tTG表达的平均灰度值为137.0±2.6,与正常玻璃体液组(143.5±2.9)及PBS对照组(143.6±3.0)相比,表达水平升高(P<0.05).结论:PVR视网膜前膜和培养的人RPE细胞tTG表达阳性,在PVR患者的玻璃体液作用下,RPE细胞的tTG表达水平升高,这表明tTG参与了PVR的发病过程,在PVR视网膜前膜的形成过程中可能有重要作用.  相似文献   

9.
人视网膜前膜中粘附分子的免疫组织化学观察   总被引:4,自引:1,他引:3  
目的 观察增生性玻璃体视网膜病变(Proliferative vitreoreti nopathy,PVR)的视网膜前膜(epiretinal membranes,ERM)中粘附分子(ICAM-1,Mac-1) 的表达。方法用免疫组织化学方法对20例复杂视网膜脱离行玻 璃体切割术时剥离的视网膜前膜进行观察。结果ICAM-1和Mac-1分别在18和15例视网膜前膜中表达,两种粘附分子同在12例视网膜前膜中表达。结论ERM中有ICAM-1和Mac-1的表达,提示粘附分子在PVR的发 生发展中有一定作用。(中华眼底病杂志,2000,16:71-138)  相似文献   

10.
目的比较研究增生性玻璃体视网膜疾病PVR及PDR增生膜组织超微结构与生长因子受体表达的生物学特点。方法PVR、PDR增生膜组织标本29例,通过电镜与免疫组织化学方法比较研究PVR与PDR增生膜组织超微结构、细胞膜上TGFβR1和EGF受体表达的生物学特点。结果(1)PVR、PDR增生膜中细胞类型主要是RPE细胞、纤维细胞、纤维母细胞样细胞和巨噬细胞;PVR与PDR膜组织超微结构不同。(2)TGFβR1受体在PDR增生膜组织中的表达,其平均光强度(P<0.01)与平均阳性面积(P<0.05)均强于PVR增生膜组织,EGF受体在PDR增生膜组织中(13/14,92.8%)表达较PVR增生膜组织中(3/15,20%)明显(P<0.05)。(3)无论PDR、PVR增生膜组织TGFBRI受体在病程>6个月的增生膜组织中的表达,明显强于小于6个月的标本(P=0.002),而EGF受体在病程2-6个月的膜组织中表达平均面积(P=0.024)明显小于其他病程的膜组织。结论PDR、PVR增生膜组织,其超微结构与生长因子表达的生物学特点不同。  相似文献   

11.
实验性增殖性玻璃体视网膜病变的膜形成及超微结构观察   总被引:2,自引:0,他引:2  
王丽娜  梁树今 《眼科研究》1993,11(1):6-8,T002
将培养的异体兔皮成纤维细胞注入兔眼玻璃体中成功地形成了增殖性玻璃体视网膜病变(PVR)。术后28天牵引性视网膜脱离发生率为73%。结果显示增殖膜主要由成纤维细胞、神经胶质细胞、肌成纤维细胞及巨噬细胞构成。玻璃体内可见大量的淋巴细胞、单核细胞及浆细胞。文中详细观察了增殖膜形成过程,以及增殖膜的超微结构,并对实验性PVR的发生机理进行了初步的探讨。  相似文献   

12.
目的 观察药物性玻璃体后脱离(PVD)对增生性玻璃体视网膜病变(PVR)的影响。方法 24只有色成年家兔,左眼为实验眼。兔自体富含血小板血浆玻璃体腔注射建立兔眼PVR模型,同时随机将实验兔分为A、B组及对照组,每组各8只眼。建模后3 h A组玻璃体腔内注入1 U纤溶酶(0.05 ml)+20 U透明质酸酶(0.05 ml)共0.1 ml、B组玻璃体腔内注入纤溶酶0.1 ml、对照组玻璃体腔内注入等量的平衡盐溶液。给药后1、7、28 d记录实验眼PVR级别,进行各组PVR等级评分,并行闪光视网膜电图(F-ERG)、眼部B型超声检查及视网膜组织病理学检查。结果 PVR模型成功建立,并在注药后7 d,A组发生完全性PVD 5只眼,不完全性PVD3只眼;B组不完全性PVD 5只眼,未见完全性PVD,另3只及对照组无PVD发生。A、B组在注药后28 d PVR等级评分均低于对照组,差异有统计学意义(D=75.6, 98.9;P=0.003,P=0.011);注药后7、28 d,A、B 两组F-ERG b 波波幅均高于对照组;产生PVD的眼中PVR级别均较无PVD的眼级别低,其中完全性PVD眼中PVR级别仅为0~1级。结论 在兔眼PVR建模后3 h,纤溶酶与透明质酸酶联合玻璃体腔注射诱导的完全性PVD在一定程度上可以阻止兔眼PVR的发生和发展,纤溶酶单独或联合透明质酸酶玻璃体腔注射诱导的不完全 性PVD对PVR的发展有减缓作用。  相似文献   

13.
金丝桃素抑制兔外伤性增生性玻璃体视网膜病变   总被引:8,自引:0,他引:8  
目的:研究蛋白激酶C(protein kinase C,PKC)特异性抑制剂—金丝桃素对外伤性增生性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)的防治作用机制。方法:用自体富含血小板血浆玻璃体内注射制备免外伤性PVR模型,并同时将生理盐水、1μM、10μM和100μM金丝桃素0.1ml注入兔眼玻璃体内,在不同时间点观察金丝桃素对PVR形成的防治作用。结果:在对照组第10d就出现视网膜脱离,PVR随着时间延长发展至更严重等级。在金丝桃素组,PVR也发生,但其严重程度在每个时间点均低于对照组。在10μM和100μM金丝桃素组给药5d后,PVR的临床分级显著低于对照组(P<0.05)。组织学检查金丝桃素组未发现明显视网膜形态改变,视网膜电图检查也未观察到显著功能变化。结论:金丝桃素玻璃体腔内注射是安全的,并能有效抑制兔外伤性PVR的发生发展,为将来临床应用提供可靠的理论依据。眼科学报 2002;18:240-246.  相似文献   

14.
The cell injection model of proliferative vitreoretinopathy (PVR) in the rabbit was used to study the therapeutic value of intravitreal adriamycin (doxorubicin). Adriamycin in a dose of 10 nmol per eye, if injected at the same time as the cells, controls PVR. At the cell doses used, PVR is not affected if there is a time interval between cell and drug injection. Because of retinal toxicity, as evidenced by electroretinographic and histopathologic changes, the beneficial effects of adriamycin on membrane formation cannot be exploited at this time.  相似文献   

15.
The effectiveness of antimetabolic and anticollagen agents against proliferative vitreoretinopathy (PVR) was assessed by vitreous microtensiometry, a new technique that measures in situ the tensile strength of vitreous membranes. Two PVR models were produced in rabbits by intravitreal injection of bovine retinal pigment epithelial (RPE) or fibroblast cells, and the animals subsequently were treated with 5-fluorouracil (5-FU) and beta-aminopropionitrile (BAPN), administered alone or in combination. The fibroblast PVR model produced high-strength membranes that did not respond significantly to these therapies. The RPE model gave lower-strength membranes that showed marginally significant decreases in strength with intravitreal 5-FU and systemic BAPN treatments. However, combination therapy showed a highly significant decrease in membrane strength and a clinically encouraging reduction in retinal detachment.  相似文献   

16.
PURPOSE. Hypericin, a polycyclic dione used as an antidepressant, has been shown to inhibit the protein kinase C (PKC) pathway. Many of the pathologic responses found in proliferative vitreoretinopathy (PVR) are dependent upon PKC. Therefore, we studied the effect of hypericin on the treatment of experimental PVR. METHODS. PVR was induced in pigmented rabbits by intravitreal injection of 50,000 rabbit conjunctival fibroblasts after vitrectomy. Subsequently, the eyes received an intravitreal injection of either balanced salt solution (BSS, 0.1 mL) (group A, control) or hypericin (0.1 mL) in doses of 1 muM (group B), 10 muM (group C), and 100 muM (group D). The eyes were examined ophthalmoscopically on days 1, 3, 7, 14, and 28 after surgery and the stage of PVR was evaluated (0 to V). The effect of hypericin on retinal morphology and function was also determined for the eyes injected with 100 muM hypericin with no fibroblasts by light microscopy and electroretinogram (ERG). RESULTS. In the control eyes, the retina was detached after 5 days, membranes had formed on and beneath it, and the PVR had progressed to higher stages over time. In the eyes injected with hypericin, the PVR also progressed; however, the severity of PVR on each day was lower than that in control eyes on that day. PVR was significantly inhibited in groups C and D as compared with the control eyes after day 5 (P < 0.05). Histological examination of the hypericin-treated control eyes disclosed no morphological change, and ERG analysis revealed no significant functional change. CONCLUSIONS. Intravitreal injection of hypericin is a safe and effective means of reducing experimental PVR.  相似文献   

17.
背景前期研究发现,激肽原1是增生性玻璃体视网膜病变(PVR)患者玻璃体中的特异蛋白质,是在质谱鉴定中得到的肽段匹配和MASCOT得分最高的蛋白质,且与PVR的严重程度呈正相关。目的探讨激肽原一激肽系统(KKS)是否参与PVR的发生过程。方法大鼠视网膜色素上皮(RPE)细胞系RPE-J细胞复苏培养后用PBS配制成2.5X10^8/ml的细胞悬液,大鼠下腔静脉采血4ml经枸橼酸二钠处理和离心后用PBS制备成血小板密度为2.5×10^8/ml的血浆。用随机数字表法将60只Wistar大鼠随机分为实验组和生理盐水组,每组各30只。实验组大鼠左眼玻璃体腔内注射RPE细胞悬液4ill+富含血小板血浆6仙l建立PVR模型,生理盐水组左眼玻璃体腔以同样的方法注射生理盐水10μl,各组大鼠的右眼作为自身对照组。术后1、3、7、14、21、28d行裂隙灯及间接检眼镜检查,按Francine的标准进行PVR分级。玻璃体腔注射后28d收集实验动物的玻璃体、血清和视网膜标本,应用Westernblot法检测缓激肽的表达,视网膜标本行组织病理学检查。结果术后28d,实验组有25只眼出现不同程度的PVR表现,建模成功率为89.3%(25/28)。术后7、14、28d裂隙灯下证实实验组大鼠形成1、2、3级PVR。视网膜组织病理学检查表明视网膜组织中炎性细胞浸润及RPE细胞移行并转化为成纤维细胞,出现视网膜脱离。Westernblot分析显示在所有PVR大鼠血清、玻璃体和视网膜中均可检测到缓激肽的表达,但实验组大鼠的表达强度明显高于生理盐水组大鼠。结论玻璃体腔注射RPE细胞联合富含血小板血浆的方法可以成功建立PVR大鼠模型,KKS可能参与PvR的发生。  相似文献   

18.
PURPOSE: To develop a system for inducible photoreceptor-specific gene expression in transgenic mice. The tetracycline regulatory system was chosen because it possesses the useful property of direct control of gene expression through use of an exogenous agent, doxycycline, a tetracycline derivative. METHODS: Transgenic mice were generated that carried the reverse tetracycline-controlled transactivator under the control of the photoreceptor-specific promoters for rhodopsin and interphotoreceptor retinoid-binding protein. These animals were crossed with transgenic mice carrying the lacZ reporter gene under control of the tetracycline operator cassette, creating doubly transgenic mice. Doxycycline was administered to induce expression of the reporter gene. Reporter assays were then performed to evaluate lacZ expression. RESULTS: Doxycycline administration led to photoreceptor-specific expression of the lacZ reporter gene in the doubly transgenic mice. X-gal staining was restricted to photoreceptor inner segments and synaptic termini. Induction could be achieved by addition of the drug to the animals' drinking water or by intravitreal injection. Induction was noted within 24 hours of doxcycline administration. Because of variability among animals, there was an approximate correlation, but not a clean dose-response curve relating drug dose to level of reporter expression. CONCLUSIONS: A transgenic system for inducible photoreceptor-specific gene expression has been developed. This system is currently being exploited to study the effects of regulated expression of genes of biological interest.  相似文献   

19.
We established an effective animal experimental model for proliferative vitreoretinopathy (PVR) by intravitreally injecting cultured cells in order to investigate therapies for PVR, including intravitreal drugs, radiation and hyperthermia. After making posterior vitreal separation by injecting SF6 gas into the rabbit's vitreous body, cultured cells were intravitreally injected. Fundus changes were followed up for 4 weeks and PVR stage was recorded according to the classification described by Hida et al. The cultured cells injected were rabbit dermal fibroblasts (5 x 10(4) and 10 x 10(4) and human retinal pigment epithelial (RPE) cells (5 x 10(4]. A total of 37% of animals reached STAGE 5-7 (traction retinal detachment) in 4 weeks in 41 eyes injected with 5 x 10(4) rabbit fibroblasts and were 86% in 14 eyes injected with 10 x 10(4) rabbit fibroblasts. The 10 eyes injected with human RPE cells showed STAGE 2 or less in 4 weeks. Consequently, we selected the experimental PVR model using injecting of 10 x 10(4) rabbit fibroblasts as the most effective method.  相似文献   

20.
目的观察玻璃体腔注射阿霉素和万古霉素对感染性眼内炎及外伤性增生性玻璃体视网膜病变(PVR)的抑制效果。方法40只新西兰白兔随机分为4组,每组10只。右眼建立外伤性出血性眼球穿孔伤模型,左眼为空白对照眼。4个组中生理盐水组,玻璃体腔注射生理盐水0.1mL;阿霉素组,注射阿霉素2.5μg(0.1mL);万古霉素组,注射万古霉素1.0mg(0.1mL);联合用药,注射阿霉素2.5μg(0.1mL)及万古霉素1.0mg(0.1mL)。以裂隙灯显微镜观察眼前段炎症情况,炎症持续超过2周者行玻璃体微生物学培养;直接检眼镜观察外伤性PVR情况。结果联合用药组PVR程度低于生理盐水组(P=0.023)及万古霉素组(P=0.034);生理盐水组、阿霉素组各发生细菌性眼内炎2例(20.0%);万古霉素组、联合用药组未见细菌性眼内炎发生。结论在外伤性出血性眼球穿孔伤动物模型中,玻璃体腔注射阿霉素可能降低外伤性PVR程度;而玻璃体腔注射万古霉素可能降低感染性眼内炎症发生率。  相似文献   

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