冠心病患者细胞间粘附分子-1、P-选择素和von Willebrand因子的表达

探讨细胞间粘附分子－１（ＩＣＡＭ－１）、Ｐ－选择素（Ｐ－ｓｅｌｅｃｔｉｎ）．ｖｏｎＷｉｌｌｅｂｒａｎｄ因子（ｖＷＦ）在冠心病中的表达特点和临床意义。方法用酶联免疫吸附法（ＥＬＩＳＡ）检测２８例稳定性心绞痛患者（ＳＡ）、２３例不稳定性心绞痛患者（ＵＡ）和２０例心肌梗死患者（ＡＭＩ）血中ＩＣＡＭ－１、Ｐ－ｓｅｌｅｃｔｉｎ、ｖＷＦ的表达。结果冠心病患者血中ＩＣＡＭ－１、Ｐ－ｓｅｌｅｃｔｉｎ和ｖＷＦ的表达较正常组增高,且在ＵＡ组中的表达高于ＳＡ组,ＡＭＩ组的表达高于ＵＡ组。除Ｐ－ｓｅｌｅｃｔｉｎ在ＳＡ组与正常组间差别不显著外,其余均差异显著（Ｐ＜０．０１）。ＩＣＡＭ－１和ｖＷＦ在ＳＡ、ＵＡ及ＡＭＩ组均呈正相关。结论ＩＣＡＭ－１、Ｐ－ｓｅｌｅｃｔｉｎ和ｖＷＦ在不同类型冠心病患者有不同的增加,表明它们与冠心病的发生、发展及临床严重程度密切相关。     

冠心病患者细胞间粘附分子—1,P—选择素和von Willebrand因子的 …

目的 探讨细胞间粘附分子－１（ＩＣＡＭ－１）、Ｐ－选择素（Ｐ－ｓｅｌｅｃｔｉｎ）、ｖｏｎ Ｗｉｌｌｅｂｒａｎｄ因子在冠心病中的表达特点和临床意义。方法 用酶联吸附法检测２８例稳定性心绞痛患者、２３例不稳定性心绞痛患者和２０例心肌梗死患者血中ＩＣＡＭ－１、Ｐ－ｓｅｌｅｃｔｉｎ、ｖＷＦ的表达。结果 冠心病患者血中ＩＣＡＭ－１、－ｓｅｌｅｃｔｉｎ和ｖＷＦ的表达较正常组增高,且在 ＵＡ组中的表达高于ＳＡ组     

肿瘤患者血浆可溶性P—选择素的检测及意义

探讨肿瘤患者血浆可溶性Ｐ－选择素（Ｐ－ｓｅｌｅｃｔｉｎ）检测的意义。方法用ＥＬＩＳＡ法检测１１６例不同肿瘤患者血浆中可溶性Ｐ－ｓｅｌｅｃｔｉｎ的含量。结果各组肿瘤患者血浆可溶性Ｐ－ｓｅｌｅｃｔｉｎ均明显高于正常人对照组（Ｐ＜０．０５或Ｐ＜０．０１）。结论肿瘤患者血浆可溶性Ｐ－ｓｅｌｅｃｔｉｎ含量与正常人有差异，检测可溶性Ｐ－ｓｅｌｅｃｔｉｎ有可能成为肿瘤辅助诊断的一项有价值的指标。     

自身免疫病患者血清胞间粘附分子检测的意义探讨

目的了解进行性硬皮病（ＰＳＳ）、类风湿性关节炎（ＲＡ）、系统性红斑狼疮（ＳＬＥ）血清可溶性细胞间粘附分子（ｓＩＣＡＭ）１和３的水平及与病情的关系。方法用进口酶联免疫吸附法（ＥＬＩＳＡ）试剂盒分别检测活动期ＰＳＳ、ＲＡ、ＳＬＥ患者与正常人血清ｓＩＣＡＭ－１以及ＰＳＳ、ＲＡ患者、正常人血清ｓＩＣＡＭ－３的含量，比较其水平的差异。结果正常对照组（２０例）ｓＩＣＡＭ－１和ｓＩＣＡＭ－３水平分别为２０２．３５±７４．１２μｇ／Ｌ和３６．１８±１４．６８μｇ／Ｌ；ＰＳＳ组患者（３０例）分别为４１３．９２±１４４．５３μｇ／Ｌ和４７．５０±２６．６８μｇ／Ｌ；ＲＡ组患者（２５例）分别为４０５．８８±１８１．１６μｇ／Ｌ和２９．２５±１１．６４μｇ／Ｌ；ＳＬＥ组患者（１９例）ｓＩＣＡＭ－１的水平为３８９．０６±２４１．９４μｇ／Ｌ。各患者组的ｓＩＣＡＭ－１水平与正常对照组比较差异有非常显著性（Ｐ＜０．０１）；ＰＳＳ、ＲＡ组患者与正常对照组ｓＩＣＡＭ－３测值相互间差异无显著性，但ＰＳＳ与ＲＡ组患者间差异有显著性（Ｐ＜０．０５）。结论ｓＩＣＡＭ－１与自身免疫病的活动性呈一致性，ｓＩＣＡＭ－３在不同的自身免疫病有不同的表现     

病毒性肝炎肝纤维化患者血浆的细胞间粘附分子—1测定及其临床意义

目的探讨细胞间粘附分子－１（ＩＣＡＭ－１）与病毒性肝炎肝纤维化的关系。方法用ＥＬＩＳＡ法检测了３９例病毒性肝炎肝纤维化患者和３０例健康人血浆ｓＩＣＡＭ－１、Ⅳ型胶原、层粘连蛋白含量。结果显示病毒性肝炎肝纤维化患者血浆ｓＩＣＡＭ－１、Ⅳ型胶原、层粘连蛋白水平显著高于健康组，且在肝纤维化分级中呈ｃｈｉｌｄ Ｃ＞ｃｈｉｌｄ Ｂ＞ｃｈｉｌｄ Ａ，相关性分析显示ｓＩＣＡＭ－１与Ⅳ型胶原、层粘连蛋白水平呈正相关（ｒ＝０．７９～０．８９，ｒ＝０．６２～０．７３）。结论病毒性肝炎肝纤维化患者血浆ｓＩＣＡＭ－１升高与肝细胞损伤有关，可反映肝纤维化的严重程度。     

过敏性鼻炎患者血清sICAM—1水平测定及其意义

目的：了解过敏性鼻炎患者血清中可溶性细胞间粘附分子（ｓＩＣＡＭ）－１水平及意义。方法：采用抗体夹心ＥＬＩＳＡ法对３１例过敏性鼻炎患者血清ｓＩＣＡＭ－１水平进行测定。结果：过敏性鼻炎患者血清中ｓＩＣＡＭ－１水平与正常对照组比较明显增高（Ｐ〈０．００１）。结论：ｓＩＣＡＭ－１参与了过敏性鼻炎发病过程,且与临床症状有一定平行关系,推测ｓＩＣＡＭ－１可能在过敏性鼻炎炎症的加重与持续发生中有一定意义。     

肾小球肾炎患者WBC流变性和CD18表达及血清sICAM—1浓度测定及其意义

探讨ＷＢＣ流变性和细胞粘附分子（ＣＡＭｓ）与肾小球肾炎病情变化的关系。方法采用红细胞变形能力测定仪、体外。栓血小板粘附两用仪及酶联免疫吸附法（ＥＬＩＳＡ）,分别检测了４０例急性肾小球肾炎患者、４２例慢性肾小球肾炎患者和４５例健康人外周血白细胞滤过指数（ＬＥＩ）、白细胞粘附率（ＬＡＲ）、白细胞ＣＤ１８表达及血清可溶性细胞间粘附分子一１（ＳＩＣＡＭ－１）浓度的变化。结果肾小球肾炎患者ＬＦＩ,ＬＡＲｔ,白细胞ＣＤ１８表达和ｓＩＣＡＭ－１浓度均明显增高,与对照组比较差异有极显著性（Ｐ均＜０．００１）,且慢性肾小球肾炎患者各指标增高较急性肾小球肾炎患者更明显（Ｐ均＜０．００１）。结论白细胞流变性异常及ＣＤ１８表达、血清ｓＩＣＡＭ－１浓度增高与肾小球肾炎病情变化有密切关系。     

自身免疫病患者血清胞间粘附分子检测的意义探讨

目的 了解进行性硬皮病（ＰＳＳ）、类风湿性关节炎（ＲＡ）、系统性红斑狼疮（ＳＬＥ）血清可溶性细胞间粘附分子（ｓＩＣＡＭ）１和３的水平及与病情的关系。方法 用进口酶联免吸附法（ＥＬＩＳＡ）试剂盒分别检测活动期ＰＳＳ、ＲＡ、ＳＬＥ患者与正常人血清ｓＩＣＡＭ－１以及ＰＳＳ、ＲＡ患者、正常人血清ｓＩＣＡＭ－３的含量，比较其水平的差异。结果 正常对照组（２０例）ｓＩＣＡＭ－１和ｓＩＣＡＭ－３水平分别为２０２     

肝硬化患者血清可溶性细胞间粘附分子—1水平变化及意义

目的；探讨肝硬化患者血清可溶性细胞粘附分子－１（ｓＩＣＡＭ－１）水平变化，了解它与肝硬化患者肝功能损伤和门脉高压形成的关系。方法：用酶联免疫吸附法（ＥＬＩＳＡ）检测１７例正常人和３３例肝硬化患者血清ｓＩＣＡＭ－１。结果；肝硬化组血清ｓＩＣＡＭ－１水平显著高于对照组，且在肝功能分级中，呈现Ｃｈｉｌｄ Ｃ〉Ｃｈｉｌｄ Ｂ〉Ｃｈｉｌｄ Ａ的规律。相关分析显示肝硬化者血清ｓＩＣＡＭ－１与总胆红素及谷丙转氨     

N—甲基天冬氨酸受体拮抗剂AP—5在海马对兔P3波电位的 …

目的 推测Ｎ－甲基天冬氨酸受体（Ｎ－ｍｅｔｈｙｌ－Ｄ－ａｓｐａｒａｔｅ ｒｅｃｅｐｔｏｒ,ＮＭＤＡＲ）的兴奋性突触后电位（ｅｘｃｉｔａｔｏｒｙ ｐｏｓｔｓｙｎａｐｔｉｃ ｐｏｔｅｎｔｉａｌｓ,ＥＰＳＰ）活动对兔Ｐ３波的作用。方法 采用ＮＭＤＡＲ竞争性拮抗剂ＡＰ－５（３．１２５,６．２５,１２．５ｍｍｏｌ／Ｌ）在海马ＣＡ１,ＣＡ３微量注入,观察Ｐ３波电位变化。     

Effect of 5-fluorouracil, mitoxantrone, methotrexate, and vincristine on the antibacterial activity of ceftriaxone, ceftazidime, cefotiam, piperacillin, and netilmicin

Using the checkerboard agar dilution technique, antibacterial activity and in vitro interactions of 4 antineoplastic agents and 5 antimicrobial drugs were examined against 56 strains of 7 bacterial species. 5-fluorouracil was found to inhibit all strains of Staphylococcus aureus and of Staphylococcus epidermidis at a concentration of 0.8 micrograms/ml or less. 84% of all gram-negative strains were inhibited synergistically when 5-fluorouracil was combined with beta-lactam antibiotics. Methotrexate and cefotiam were antagonistic in 42% of all combinations, especially when tested against Escherichia coli and Klebsiella pneumoniae.     

Susceptibilities of non-Pseudomonas aeruginosa gram-negative nonfermentative rods to ciprofloxacin, ofloxacin, levofloxacin, D-ofloxacin, sparfloxacin, ceftazidime, piperacillin, piperacillin-tazobactam, trimethoprim-sulfamethoxazole, and imipenem.

Agar dilution MICs of 10 agents against 410 non-Pseudomonas aeruginosa gram-negative nonfermentative rods were determined. MICs at which 50 and 90% of the isolates were inhibited, respectively, were as follows (in micrograms per milliliter): sparfloxacin, 0.5 and 8.0; levofloxacin, 1.0 and 8.0; ciprofloxacin, 2.0 and 32.0; ofloxacin, 2.0 and 32.0; D-ofloxacin, 32.0 and > 64.0; ceftazidime, 8.0 and 64.0; piperacillin with or without tazobactam, 16.0 and > 64.0; trimethoprim-sulfamethoxazole, 0.5 and > 64.0; imipenem, 2.0 and > 64.0. With the exception of those for Stenotrophomonas maltophilia, Burkholderia cepacia, and Alcaligenes faecalis-A. odorans, agar dilution MICs for all strains tested were within 1 dilution of inhibitory (bacteriostatic) levels as determined by time-kill methodology.     

Comparative ototoxicity of ribostamycin, dactimicin, dibekacin, kanamycin, amikacin, tobramycin, gentamicin, sisomicin and netilmicin in the inner ear of guinea pigs

Nine aminoglycoside antibiotics, ribostamycin (RSM), dactimicin (DAC), dibekacin (DKB), kanamycin (KM), amikacin (AMK), netilmicin (NTL), tobramycin (TOB), gentamicin (GM) and sisomicin (SISO) were administered intramuscularly to guinea pigs for 4 weeks, and ototoxicity and drug concentration in the inner ear fluid were determined. RSM and DAC showed the weakest ototoxicity against the cochlea and vestibular organs. AMK and KM were more toxic to cochlea than vestibular organs. DKB, TOM, GM and SISO were equally toxic to vestibular organs and cochlea. NTL was more toxic to vestibular organs than cochlea. As judged from the pinna reflex response and hair cell damage in the cochlea, the order of auditory toxicity was the following: SISO greater than GM greater than TOB greater than AMK greater than DKB greater than KM greater than NTL, DAC RSM, whereas the vestibular toxicity was in the following order: SISO greater than GM greater than DKB greater than TOB greater than NTL greater than AMK greater than KM greater than DAC, RSM. RSM, causing the weakest ototoxicity, showed a low drug concentration in the inner ear fluid, while GM, causing severe ototoxicity, showed the highest drug level under the same conditions.     

Reviews of books, videos, CDs, audiotapes, web sites, and more, written by emergency nurses

Zimmermann PG. Philadelphia: Hanley & Belfus, 2002, 243 pp, ISBN 1-56053-529-6.