共查询到20条相似文献,搜索用时 46 毫秒
1.
Since the publication of the 2001 and 2008 World Health Organization classifications of tumours of haematopoietic and lymphoid tissues, there has been an increasing move towards classification of haematological neoplasms on the basis of the underlying molecular genetic disorder. In recent decades there have been a significant number of reports of haematological neoplasms with rearrangement of JAK2. Published data on such cases have therefore been analysed to determine if any specific entities could be identified. On the basis of this analysis, it is suggested that lymphoid and myeloid neoplasms associated with t(8;9)(p22;p24); PCM1‐JAK2 fusion should be recognized as an entity. Furthermore, lymphoid and myeloid neoplasms associated respectively with t(9;12)(p24;p13); ETV6‐JAK2 and with t(9;22)(p24;q11·2); BCR‐JAK2 should be documented carefully in order to define their features more clearly and assess whether they can be recognized as entities. Identification of all these conditions is important because of the possibility of response to JAK2 inhibitors. 相似文献
2.
Relative expression analysis of IL‐5 and IL‐6 genes in tropical sheep breed Pelibuey infected with Haemonchus contortus
下载免费PDF全文
![点击此处可从《Parasite immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Z. M. Estrada‐Reyes A. G. López‐Reyes A. Lagunas‐Martínez G. Ramírez‐ Vargas S. Olazarán‐Jenkins J. Hernández‐Romano P. Mendoza‐de‐Gives M. E. López‐Arellano 《Parasite immunology》2015,37(9):446-452
Haemonchus contortus is a parasitic nematode of Pelibuey sheep, a meat breed used in tropical regions. Due to anthelmintic problems, the identification of hosts resistant to H. contortus is another option of control. The aim of this study was to analyse the relative expression of IL‐5 and IL‐6 genes in Pelibuey sheep after H. contortus infection. Nineteen lambs infected with H. contortus and three more lambs without infection were studied. The haemonchosis was determined by the number of eggs per gram of faeces (epg) and by the estimation of the percentage of the packed cell volume (%pcv). Peripheral blood mononuclear cells (PBMCs) were obtained to extract RNA at 0, 1, 2, 7, 14, 21 and 28 days after infection to quantify the relative expression of IL‐5, IL‐6 and GAPDH by real‐time PCR. Five lambs were classified as low responders (lr) to haemonchosis with averages of 1519 ± 315·3 epg and 31·49 ± 5·13%pcv, and 14 lambs were identified as high responders (hr) with averages of 530 ± 132 epg and 34·88 ± 3·75%pcv. The expression ratio of IL‐5 was significantly different compared with control lambs at 2, 7 and 14 days post‐infection (PI), and IL‐6 was significantly different after 14 days. The highest level of relative expression for IL‐5 and IL‐6 genes was 9·9‐fold and 12‐fold after 2 and 14 days for hr hosts (P < 0·05) compared with control group, respectively. In conclusion, the Pelibuey breed in grazing areas exhibited different expression of IL‐5 and IL‐6 obtained from PBMCs against H. contortus, suggesting the importance of these cytokines in regulating the nematode infection. 相似文献
3.
Effect of once‐weekly dulaglutide on glycated haemoglobin (HbA1c) and fasting blood glucose in patient subpopulations by gender,duration of diabetes and baseline HbA1c
下载免费PDF全文
![点击此处可从《Diabetes, obesity & metabolism》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Baptist Gallwitz MD Samuel Dagogo‐Jack MD Vivian Thieu PhD Luis‐Emilio Garcia‐Perez MD PhD Imre Pavo MD Maria Yu MS Kenneth E. Robertson PharmD Nan Zhang PhD Francesco Giorgino MD 《Diabetes, obesity & metabolism》2018,20(2):409-418
Aims
To evaluate the efficacy and safety of dulaglutide 1.5 and 0.75 mg in patients with type 2 diabetes by subgroups of gender, duration of diabetes and baseline glycated haemoglobin (HbA1c) in the dulaglutide clinical development programme (AWARD‐1 to ‐6 and ‐8 clinical trials).Methods
Change in HbA1c was analysed by gender, duration of diabetes (<5, ≥5 years and <10, ≥10 years), and baseline HbA1c (<8.5%, ≥8.5%) in pooled and individual studies. Changes from baseline in weight, hypoglycaemia and gastrointestinal adverse events were evaluated for individual trials.Results
In the pooled analysis of patients treated with dulaglutide 1.5 mg at 6 months, the reductions in HbA1c from baseline were similar across gender (men: least squares [LS] mean ?1.26% [95% confidence interval {CI} ?1.36, ?1.16]; women: LS mean ?1.33% [95% CI ?1.43, ?1.24]) and among duration of diabetes subgroups (<5 years: LS mean ?1.32% [95% CI ?1.43, ?1.22]; ≥5 and <10 years: LS mean ?1.33% [95% CI ?1.43, ?1.22]; ≥10 years: ?1.24% [95% CI ?1.35, ?1.14]). Patients with baseline HbA1c ≥8.5% had greater HbA1c reductions than patients with baseline HbA1c <8.5%, (≥8.5%: LS mean ?1.86% [95% CI ?1.97, ?1.75]; <8.5%: LS mean ?1.02% [95% CI ?1.12, ?0.93]). Reductions in fasting blood glucose (FBG) were consistent with HbA1c changes. Similar results were observed with dulaglutide 0.75 mg. In general, body weight changes were similar among duration of diabetes and in baseline HbA1c subgroups, respectively; women had a numerically greater weight loss or less weight gain than men with both dulaglutide doses. There was no clinically meaningful difference in hypoglycaemia trends by gender or duration of diabetes. Hypoglycaemia incidence and rate were generally lower in patients with baseline HbA1c ≥8.5% than in those with <8.5%, except for the AWARD‐4 study (combination with mealtime insulin).Conclusions
Across the AWARD studies, dulaglutide demonstrated significant improvements in glycaemic control irrespective of gender, duration of diabetes, or baseline HbA1c, with greater HbA1c and FBG reductions in patients with a higher baseline HbA1c. Dulaglutide was well tolerated, with a safety profile similar to other glucagon‐like peptide‐1 receptor agonists. 相似文献4.
Tiziana Ottone Serena Zaza Mariadomenica Divona Syed Khizer Hasan Serena Lavorgna Serena Laterza Laura Cicconi Paola Panetta Jonny Di Giandomenico Michela Cittadini Claudia Ciardi Enrico Montefusco Annibale Franchi Luciana Annino Adriano Venditti Sergio Amadori Francesco Lo‐Coco 《British journal of haematology》2013,161(4):533-540
FLT3 internal tandem duplication (ITD) mutations are frequently detected at diagnosis in cytogenetically normal acute myeloid leukaemia (CN‐AML) and predict unfavourable outcome. FLT3 ITD is an unstable aberration and may be lost or acquired at relapse. Recent whole genome sequencing studies have suggested that FLT3 ITD+ve AML relapse may evolve from small subclones undetectable at diagnosis by routine polymerase chain reaction (PCR). We developed a patient‐specific real‐time quantitative‐PCR (RQ‐PCR) to implement FLT3 ITD detection in six AML patients whose blasts carried wild‐type FLT3 at diagnosis and who relapsed with FLT3 ITD by routine PCR. Patient‐specific forward primers were designed after cloning and sequencing the FLT3 ITD in each case. The assay allowed retrospective detection of FLT3 ITD in diagnostic samples of 4/6 cases and to establish the kinetics of clonal evolution preceding relapse. After conventional chemotherapy, all patients had early relapse despite having been classified as NPM1+ve/FLT3 ITD?ve at presentation, with shorter remissions being observed in four patients re‐classified as FLT3 ITD+ve by the new assay. Notably, FLT3 ITD clone became detectable by conventional PCR in three patients tested during remission after initial treatment. Our data underscore the need of identifying low FLT3 ITD levels, which are probably associated with relapse in otherwise good prognosis CN‐AML. 相似文献
5.
6.
Comparison of glucose‐lowering agents after dual therapy failure in type 2 diabetes: A systematic review and network meta‐analysis of randomized controlled trials
下载免费PDF全文
![点击此处可从《Diabetes, obesity & metabolism》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Francesco Zaccardi MD Nafeesa N. Dhalwani PhD Jolyon Dales BM BS Hamid Mani PhD Kamlesh Khunti FRCGP Melanie J. Davies FRCP David R. Webb FRCP 《Diabetes, obesity & metabolism》2018,20(4):985-997
7.
Glycaemic outcomes of an Individualized treatMent aPproach for oldER vulnerable patIents: A randomized,controlled stUdy in type 2 diabetes Mellitus (IMPERIUM)
下载免费PDF全文
![点击此处可从《Diabetes, obesity & metabolism》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Simon R. Heller MD Richard E. Pratley MD Alan Sinclair MD Andreas Festa MD Jacek Kiljański MD Cynthia S. Brusko PharmD Ran Duan PhD Robert J. Heine MD 《Diabetes, obesity & metabolism》2018,20(1):148-156
8.
Interventions to significantly improve service uptake and retention of HIV‐positive pregnant women and HIV‐exposed infants along the prevention of mother‐to‐child transmission continuum of care: systematic review
下载免费PDF全文
![点击此处可从《Tropical medicine & international health : TM & IH》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Alexandra C. Vrazo Jacqueline Firth Anouk Amzel Rebecca Sedillo Julia Ryan B. Ryan Phelps 《Tropical medicine & international health : TM & IH》2018,23(2):136-148
9.
10.
Recent studies have associated genetic variation near the interleukin 28B (IL28B/IFN‐λ3) gene with natural clearance of the hepatitis C virus (HCV) infection, and a common variant in the DEP domain containing 5 (DEPDC5) locus on chromosome 22 has been shown to affect susceptibility to hepatocellular carcinoma (HCC) in Japanese individuals with chronic HCV infection. This study was conducted to determine whether polymorphisms near or in interferon‐lambda (IFN‐λs) genes and their receptor genes such as interleukin 28 receptor, alpha (IL28RA) and interleukin 10 receptor, beta (IL10RB) as well as p21_activated kinases 4 (PAK4) and iron/zinc purple acid phosphatase‐like protein (PAPL), which are locate upstream of IFN‐λs, and lastly the DEPDC5 gene are associated with hepatitis B virus‐related liver disease in Han Chinese. The study subjects included 507 normal healthy controls, 350 individuals with natural clearance of HBV and 792 HBV‐infected patients. The patients were categorized into 157 inactive carriers (Case I), 216 active carriers (Case II), 111 cirrhotics (Case III) and 308 HCC patients (Case IV) subgroups. Seven single nucleotide polymorphisms (SNPs) were genotyped using the Matrix‐assisted Laser Desorption/Ionisation mass spectrometric (MALDI‐TOF MS) SNP genotyping assay. Rs423058 upstream of PAPL, rs2834167 in IL10RB and rs1012068 in DEPDC5 were associated with chronic HBV status, HBV natural clearance and the presence of HCC (P = 0.0004–0.024), respectively. PAPL, IL10RB and DEPDC5 polymorphisms have an impact on progression of HBV‐related liver disease. However, IFN‐λs genes as a tool to differentiate between different clinical courses of HBV infection were not useful in the Han Chinese population. 相似文献
11.
Amy Waller Alix Hall Rob Sanson‐Fisher Nicholas Zdenkowski Charles Douglas Justin Walsh 《Internal medicine journal》2018,48(1):60-66
Background
The perceptions of those called on to make decisions on behalf of patients who lack capacity at the end of life must accurately reflect patient preferences.Aims
To establish the extent to which the views of medical oncology outpatients are understood by their support persons, specifically with regards to (i) preferred type and location of end‐of‐life care, (ii) preferred level of involvement in end‐of‐life decision‐making and (iii) whether the patient has completed an advance care plan or appointed an enduring guardian.Methods
Adults with a confirmed cancer diagnosis and their nominated support persons were approached between September 2015 and January 2016 in the waiting room of an Australian tertiary referral clinic. Consenting participants completed a pen‐and‐paper survey. Nominated support persons answered the same questions from the patient’s perspective.Results
In total, 208 participants (39% of eligible dyads) participated. Observed agreement across the five outcomes ranged from 54% to 84%. Kappa values for concordance between patient–support person responses were fair to moderate (0.24–0.47) for enduring guardian, decision‐making, advance care plan and care location outcomes. A slight level of concordance (k = 0.15; 95% confidence interval: ?0.02, 0.32) was found for the type of care outcome.Conclusion
Relying on support persons’ views does not guarantee that patients’ actual preferences will be followed. Strategies that make patient preferences known to healthcare providers and support persons while they still have the capacity to do so is a critical next step in improving quality cancer care. 相似文献12.
Bianca Martins Santos Felipe Augusto Soares Stefany Laryssa Rosa Denise da Conceio Ferreira Gomes Bruno Csar Miranda Oliveira Alan Zanoni Peixinho Celso Tetsuo Nagase Suzuki Katia Denise Saraiva Bresciani Alexandre Xavier Falco Jancarlo Ferreira Gomes 《Tropical medicine & international health : TM & IH》2019,24(5):586-595
13.
14.
HLA‐DRB1 shared epitope alleles in patients with rheumatoid arthritis: relation to autoantibodies and disease severity in a south Indian population
下载免费PDF全文
![点击此处可从《International journal of rheumatic diseases》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Vasanth Konda Mohan Nalini Ganesan Rajasekhar Gopalakrishnan Vettriselvi Venkatesan 《International journal of rheumatic diseases》2017,20(10):1492-1498
15.
Benjamin Heidrich Beatriz C. Serrano Ramazan Idilman Gökhan Kabaçam Birgit Bremer Regina Raupach Fatih O. Önder Katja Deterding Behrend J. Zacher Andrzej Taranta Hakan Bozkaya Kalliopi Zachou Hans L. Tillmann Abdurrahman M. Bozdayi Michael P. Manns Cihan Yurdaydın Heiner Wedemeyer 《Liver international》2012,32(9):1415-1425
16.
H. Shindo S. Maekawa K. Komase M. Miura M. Kadokura R. Sueki N. Komatsu K. Shindo F. Amemiya Y. Nakayama T. Inoue M. Sakamoto A. Yamashita K. Moriishi N. Enomoto 《Journal of viral hepatitis》2013,20(4):281-289
Genetic variation in the IL‐28B (interleukin‐28B; interferon lambda 3) region has been associated with sustained virological response (SVR) rates in patients with chronic hepatitis C treated with peginterferon‐α and ribavirin. However, the mechanisms by which polymorphisms in the IL‐28B gene region affect host antiviral responses are not well understood. Using the HCV 1b and 2a replicon system, we compared the effects of IFN‐λs and IFN‐α on HCV RNA replication. The anti‐HCV effect of IFN‐λ3 and IFN‐α in combination was also assessed. Changes in gene expression induced by IFN‐λ3 and IFN‐α were compared using cDNA microarray analysis. IFN‐λs at concentrations of 1 ng/mL or more exhibited concentration‐ and time‐dependent HCV inhibition. In combination, IFN‐λ3 and IFN‐α had a synergistic anti‐HCV effect; however , no synergistic enhancement was observed for interferon‐stimulated response element (ISRE) activity or upregulation of interferon ‐ stimulated genes (ISGs). With respect to the time course of ISG upregulation, the peak of IFN‐λ3‐induced gene expression occurred later and lasted longer than that induced by IFN‐α. In addition, although the genes upregulated by IFN‐α and IFN‐λ3 were similar to microarray analysis, interferon‐stimulated gene expression appeared early and was prolonged by combined administration of these two IFNs. In conclusion, IFN‐α and IFN‐λ3 in combination showed synergistic anti‐HCV activity in vitro. Differences in time‐dependent upregulation of these genes might contribute to the synergistic antiviral activity. 相似文献
17.
《British journal of haematology》2017,179(2):266-271
8‐chloro‐adenosine (8‐Cl‐Ado) is currently in phase‐I clinical trials for acute myeloid leukaemia and chronic lymphocytic leukaemia (CLL). Previously, we demonstrated that treatment with 8‐Cl‐Ado leads to diminished ATP levels. We hypothesized that AMP‐activated protein kinase (AMPK) signalling would be initiated in these cells, leading to induction of autophagy. AMPK activation and induction of autophagy were demonstrated during preclinical incubations in CLL cells with the analogues. Importantly, we extended similar observations in CLL lymphocytes during an 8‐Cl‐Ado phase‐I trial. In conclusion, 8‐Cl‐Ado treatment induces autophagy in CLL lymphocytes in vitro as well as in vivo during clinical trial. 相似文献
18.
19.