C_（57）BL／6鼠日本血吸虫病肝虫卵肉芽肿模型特性初步研究

本实验对所建虫卵可溶性抗原（ＳＥＡ）致敏和未致敏Ｃ５７ＢＬ／６鼠日本血吸虫病肝虫卵肉芽肿模型特性进行初步探讨，并与Ｃ５７ＢＬ／６鼠自然感染模型进行比较。结果显示：１．经脾注射活卵诱发小鼠肝虫卵肉芽肿方法可行，２．ＳＥＡ致敏组肝虫卵肉芽肿形成过程及细胞组成基本与自然感染组相似，但发展速度较快；３．ＳＥＡ致敏组和自然感染组各期虫卵肉芽肿内ＩｇＧ抗体均呈阳性。从而证明，所建ＳＥＡ致敏Ｃ５７ＢＬ／６鼠日本血吸虫病肝虫卵肉芽肿模型对于肝虫卵肉芽肿防治研究确有价值。     

50例急性心肌梗塞患者血脂水平检测报告

目的：对脂质代谢与ＡＭＩ之间的关系进行研究。方法：采用酶法检测血清总胆固醇（ＴＣ）和甘油三酯（ＴＧ）；用磷钨酸钠Ｍｇ２＋一步沉淀法检测高密度脂蛋白（ＨＤＬＣ）及其亚组分（ＨＤＬ２Ｃ和ＨＤＬ３Ｃ）；用单向火箭负度电泳法检测载脂蛋白ＡＩ（ａｐｏＡＩ）和载脂蛋白Ｂ（ａｐｏＢ）对ＡＭＩ患者（５０例）和对照组（６０例）进行取血检测。结果：疾病组血清ＴＧ，ａｐｏＢ水平比对照组明显升高，两组比较差异有显著性（ｔ＝２．１１４，３．３８９，Ｐ均＜０．０５）；而ＨＤＬＣ，ＨＤＬ２Ｃ，ａｐｏＡＩ水平及ＨＤＬ２Ｃ／ＨＤＬ３Ｃ，ａｐｏＡＩ／ａｐｏＢ比值比对照组明显降低，两组间比较差异均有显著意义（ｔ＝１．９９６～２．７６２，Ｐ＜０．０５，０．０１）；结论：血清ａｐｏＢ水平升高可促进冠心病动脉粥样硬化的形成和发展     

鼠视网膜的免疫原性

为探讨视网膜的免疫原性,选择ＤＢＡ／２鼠（ＭＨＣ为Ｈ２ｄ）为供体,Ｃ５７ＢＬ／６（ＭＨＣ为Ｈ２ｂ）或ＢＡＬＢ／Ｃ鼠（ＭＨＣ为Ｈ２ｄ）为受体,把ＤＢＡ／２鼠的半个视网膜移植于受体鼠的皮下,２周后检测迟发型超敏反应（ＤＴＨ）和细胞毒性Ｔ淋巴细胞（ＣＴＬ）。结果示移植组的迟发型超敏反应（ＤＴＨ）和细胞介导的细胞毒效应（ＣＴＬ）均低于阳性对照ＤＢＡ／２鼠脾细胞组（Ｐ＜０．０１）,高于阴性对照假手术组（Ｐ＜０．０１）。提示ＤＢＡ／２鼠视网膜表达ＭＨＣⅠ类和Ⅱ类抗原及ＭｉｎｏｒＨＣ抗原,刺激受鼠产生较弱的细胞免疫应答。视网膜具有弱免疫原性。     

应用α—珠蛋白3′—HVR探针进行小鼠的DNA指纹分析

目的：探讨３′－ＨＶＲ探针及Ｓｏｕｔｈｅｒｎ杂交技术应用于实验小鼠遗传质量监测的可行性。方法：采用人源α－珠蛋白３′－ＨＶＲ探针,应用ＥＣＬ试剂盒,以ＨＲＰ标记进行Ｓｏｕｔｈｅｒｎ杂交,以化学发光法进行检测,对５种近交系小鼠（ＢＡＬＢ／ｃ、ＤＢＡ／２、Ｔ７３９、ＴＡ１、６１５）、１种封闭群小鼠（ＫＭ）及２种免疫缺陷小鼠（ＢＡＬＢ／ｃ－ｎｕ／ｎｕ及Ｃ．Ｂ－１７ＳＣＩＤ）进行ＤＮＡ指纹分析。结果：其不     

BALB／cA裸小鼠生长发育以及几种繁育方式的生产性能比较

比较了１雄多雌（１ｎｕ／ｎｕ×３ｎｕ／＋）及纯裸鼠交配（１ｎｕ／ｎｕ×３ｎｕ／＋）与上述两种方法兼用后的优化繁殖的生产性能。结果表明：后者母鼠的交配分娩间隔期比原来方式要短；平均胎育成裸仔数及断奶成活率均高于原来方式。另外，体生长测定显示，ＢＡＬＢ／ｃＡ裸小鼠的体生长趋势基本与ＢＡＬＢ／ｃＡ近交系小鼠相似。     

杀菌-通透性增加蛋白对烫伤大鼠脂多糖结合蛋白和CD14 mRNA表达的影响

目的观察烫伤后重组杀菌通透性增加蛋白（ｒＢＰＩ２１）对内毒素增敏系统脂多糖结合蛋白／脂多糖受体ＣＤ１４（ＬＢＰ／ＣＤ１４）及肿瘤坏死因子α（ＴＮＦα）ｍＲＮＡ表达的影响及其意义。方法采用大鼠３５％Ⅲ°烫伤模型,动物分为正常对照组（８只）、烫伤组（１２只）和ｒＢＰＩ２１治疗组（１２只）。烫伤组和ｒＢＰＩ２１治疗组动物分别于伤后１２和２４小时活杀。留取组织和血标本采用半定量ＲＴＰＣＲ方法,分别检测组织ＬＢＰ、ＣＤ１４、ＴＮＦαｍＲＮＡ表达及器官功能指标。结果ｒＢＰＩ２１治疗可明显抑制局部组织ＬＢＰ／ＣＤ１４ｍＲＮＡ表达（Ｐ＜００５００１）,并不同程度抑制肝、肺及肾组织ＴＮＦαｍＲＮＡ表达水平（Ｐ＜００５００１）。同时,ｒＢＰＩ２１治疗组动物血清谷丙转氨酶水平明显降低（Ｐ＜００１）,而小肠组织二氨氧化酶活性显著高于烫伤组（Ｐ＜００５）。结论烫伤早期应用ｒＢＰＩ２１可部分减轻肠源性内毒素移位诱发的病理损害,其作用机理可能与ｒＢＰＩ２１对体内多种组织ＬＢＰ／ＣＤ１４ｍＲＮＡ表达的下调效应有关。     

氦-氖激光血管内照射对冠心病患者血浆脂蛋白(a)的影响

选择６０例冠心病患者，分为急性心肌梗死（ＡＭＩ）、不稳定型心绞痛（ＵＡ）及稳定型心绞痛（ＳＡ）３组，均给予低强度ＨｅＮｅ激光血管内照射（ＩＬＬＬＩ）治疗。１次／ｄ，６０ｍｉｎ／次，连续１０ｄ，观察血浆脂蛋白（ａ）（Ｌｐ（ａ））、总胆固醇（ＴＣ）、高密度脂蛋白胆固醇（ＨＤＬＣ）及低密度脂蛋白胆固醇（ＬＤＬＣ）的变化。结果：ＩＬＬＬＩ治疗后冠心病各组中Ｌｐ（ａ）明显降低，同时ＴＣ及ＬＤＬＣ也有不同程度的降低，而对ＨＤＬＣ及甘油三酯（ＴＧ）无明显影响。提示：ＩＬＬＬＩ治疗可有效的降低Ｌｐ（ａ）的水平。     

不同免疫力小鼠体内肝癌细胞转移特性的变化

ＨＣａ－Ｆ２５／１６Ａ３－Ｆ（Ｆ）和ＨＣａ－Ｆ２５／Ａ２－Ｐ（Ｐ）是从小鼠腹水型肝癌细胞中分离出来的，在有正常免疫功能小鼠体内只经淋巴道转移的同源而具有不同转移力的两株克隆细胞系。将Ｆ细胞和Ｐ细胞接种到三种不同免疫功能的小鼠皮下（皮下组）和尾静脉内，它们均出现了淋巴结转移。皮下组Ｆ细胞在６１５小鼠和ＳＣＩＤ小鼠体内转移率均很高，在ＢＡＬＢ／ｃ－ｎｕ／ｎｕ小鼠体内转移率最低。Ｐ细胞在三种小鼠体内随免     

小鼠骨髓和脾来源树突状细胞的分离与扩增培养

目的：探索树突状细胞（ＤＣ）及其前体的分离纯化及其体外扩增的方法。方法：无菌制备ＢＡＬＢ／Ｃ小鼠骨髓和脾细胞；先后用红细胞裂解液、抗鼠ＣＤ４、ＣＤ８、Ｂ细胞单抗（ＭｃＡｂ）和补体溶液，依次去除红细胞，Ｔ、Ｂ细胞，粒细胞和单核巨噬细胞等混杂细胞而获得纯化的ＤＣ及其前体；又在粒细胞巨噬细胞集落刺激因子（ＧＭＣＳＦ）和白细胞介素（ＩＬ４）协同诱导下培育，ＤＣ前体分化发育成ＤＣ或郎罕细胞（ＬＣ）并扩增，同时阻抑巨噬细胞发育生长。结果：ＤＣ／ＬＣ细胞数增加，其形态在光镜下多为特征性星形，也有梭形和多角形；扫描电镜下观察其绝大多数为特征性星形，且有１～４级不等的树突状突起，其纯度高达９５％以上。ＤＣ／ＬＣ在功能上明显刺激同种异体混合淋巴细胞反应（ＭＬＲ）。结论：①结果所得细胞的形态和功能符合ＤＣ／ＬＣ；②建立连续排异结合ＧＭＣＳＦ＋ＩＬ４联合诱生培育的方法，可获得大量高纯度的ＤＣ／ＬＣ。     

反义BCR/ABL寡核苷酸体外抑制K562细胞的研究

目的：探讨反义ＢＣＲ和ＡＢＬ融合基因（ＢＣＲ／ＡＢＬ融合基因）寡核苷酸体外抑制Ｋ５６２细胞的作用及机制。方法：采用Ｋ５６２细胞培养,观察反义ＢＣＲ／ＡＢＬ寡核苷酸体外对Ｋ５６２细胞数、台盼蓝拒染率及克隆形成等的影响。结果：经反义ＢＣＲ／ＡＢＬ寡核苷酸ｂ３ａ２（ＡＳｂ３ａ２）处理后培养８ｄ的Ｋ５６２克隆抑制率达６０６９％,液体培养中细胞数从２４ｈ开始较对照组减少,细胞存活率从８ｈ开始即较对照组明显下降,ＢＣＲ／ＡＢＬ寡核苷酸ｂ２ａ２（ＡＳｂ２ａ２）及无义寡核苷酸对Ｋ５６２细胞数和存活率无明显影响;３种寡核苷酸对ＨＬ６０细胞数和存活率也无影响。结论：ＡＳｂ３ａ２对Ｋ５６２细胞有序列特异性的抑制作用。ＡＳｂ３ａ２的这种抑制作用可能在于它诱导Ｋ５６２细胞的凋亡。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.