共查询到20条相似文献,搜索用时 12 毫秒
1.
John F. Kennedy Junor A. Barnes John B. Matthews 《Clinica chimica acta; international journal of clinical chemistry》1983,129(3):251-261
Thyroxine-binding globulin (TBG) was isolated from pooled whole human serum by diethylaminoethyl (DEAE) Sephadex anion exchange chromatography followed by immunoadsorption chromatography on a cyanogen bromide-activated Sepharose 4B-sheep anti-human TBG immunoadsorbent. Sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) of the purified TBG revealed a major protein band with a molecular mass of 65000 and a weak band of molecular mass 54000 in both reducing and non-reducing buffers. Sedimentation velocity analysis revealed an S20,w coefficient of 4.5S and a calculated molecular mass of 60000. Immunochemical analysis confirmed the purity of the TBG preparation which gave a single precipitin peak on two-dimensional immunoelectrophoresis. 相似文献
2.
Paul Brown Karl D. Nolph 《Clinica chimica acta; international journal of clinical chemistry》1977,78(1):103-111
A convenient method for isolation of TBG from human pregnancy serum is reported. The method is based on batch-wise treatment of serum with hydroxyapatite followed by batch-wise adsorption of TBG to an affinity gel consisting of Sepharose to which thyroxine was bound with a spacer group. After selective desorption the TBG-preparation was practically pure. The small amounts of contaminating proteins were easily removed by affinity chromatography on Concanavalin A-Sepharose. The resulting preparation was shown to be homogeneous by polyacrylamide gel electrophoresis and immunological techniques. 相似文献
3.
R A Stockley D Burnett A R Bradwell 《Clinica chimica acta; international journal of clinical chemistry》1978,88(1):127-133
The effect of passage through the lung on alpha1-antitrypsin was studied in sixteen patients with chronic obstructive airways disease. The average mixed venous value of alpha1-antitrypsin (106.2% standard serum value) was not different from the the mean arterial value (105.6%). Where individual differences were found there was a similar difference in serum albumin. However, there was a small but significant rise (p less than 0.005) in serum alpha1-antitrypsin "complex" as blood traversed the lung (mixed value = 6.3%; mixed arterial value = 6.8%). The reason is not yet clear. 相似文献
4.
E C Klasen R J De Brij 《Clinica chimica acta; international journal of clinical chemistry》1979,95(2):391-394
Plasma samples, whose typing for alpha 1-AT is made difficult or impossible because of an excess of heparin used as anticoagulant, can be treated very effectively with protamine sulphate. The addition of this heparin antagonist results in complete clearance of the isoelectric focusing pattern. 相似文献
5.
A A Raouf M J Geisow P O'Gorman P Marsden P J Howorth 《Clinica chimica acta; international journal of clinical chemistry》1980,104(1):25-41
Pure thyroxine-binding globulin (TBG) is required in radioimmunoassay to prepare monospecific antisera, [125I]TBG and as primary standard. Homogeneous TBG was prepared by a three-stage affinity chromatography procedure; it could not be dissociated into subunits and its molecular weight by SDS polyacrylamide gel electrophoresis was 59 000. The amino acid composition was in agreement with two earlier reports. The secondary structure determined by circular dichroism in the far U.V. showed it to contain 24% each of alpha-helix and beta-pleated sheet. Serum TBG was determined by a 24-h radioimmunoassay using polyethyleneglycol to separate bound and free TBG. Serum TBG (mg/l, mean +/- S.D.) was: normal men 15.3 +/- 2.11 (n = 34), normal women 18.4 +/- 2.72 (n = 32) (P less than 0.005), in women on oral oestrogens 24.0 +/- 5.0 (n = 23), in normal pregnancy 38.6 +/- 3.0 (n =37), in cord blood 21.7 +/- 3.5 (n = 25) (P less than 0.001) and in euthyroid subjects aged over 60 years 17.8 +/- 4.5 (P n.s.). In women with thyroid disease TBG was reduced in hyperthyroidism: 15.5 +/- 2.5 (n = 28) and elevated in hypothyroidism: 21.0 +/- 4.0 (n = 25). Wider use in TBG-assay of non-denatured TBG of proven composition and structure should decrease the scatter in reference ranges and improve its value as a routine thyroid function test as both a primary measurement and as the T4 : TBG ratio. 相似文献
6.
M Parsons G Romeo 《Clinica chimica acta; international journal of clinical chemistry》1980,100(3):215-224
alpha 2-Macroglobulin was purified from plasma of five cystic fibrosis patients and five normal controls. SDS gel electrophoresis of native alpha 2-macroglobulin from cystic fibrosis patients and normal donors showed identical subunit molecular weights, as did trypsin cleavage products. Cystic fibrosis and control alpha 2-macroglobulins were indistinguishable by isoelectric focusing and exhibited appropriate shifts in isoelectric point following binding of trypsin. The trypsin-binding capacities of control and cystic fibrosis alpha 2-macroglobulins did not differ, nor did the esterolytic activity of the trypsin-alpha 2-macroglobulin complexes. 相似文献
7.
T Miyazaki T Hachiya Y Kajita Y Ochi Y Fujiyama S Hosoda 《Clinica chimica acta; international journal of clinical chemistry》1982,122(2):161-168
We have recently suggested that carcinoembryonic antigen (CEA) may contain alpha 1-acid glycoprotein (AG) antigenic determinant. In the present work we examined a protein with CEA-like activity in the feces of healthy subjects (NCA) for immunological cross-reactivity with AG. When the perchloric acid extract of feces was fractionated on a Sephadex G-200, two fractions (large and small molecular weight) were obtained. The large molecular weight fraction had higher CEA activity than the small one. The perchloric acid (PCA) extract of feces was subjected to affinity chromatography using anti-CEA bound to Sepharose, and the bound protein was labelled with 125I, and then fractionated on a Sephadex G-200 column. Two radioactive peaks, Peak 1 corresponding to an approximate Mr of 180 000 and Peak 2, corresponding to an approximate Mr of 60 000 were found. Both peaks showed immunoreactivity with either anti-CEA or anti-AG. This experiment suggests the presence of two kinds of CEA-reactive proteins in feces: one which may be a big protein with immunological similarity to AG and a second which appears to be a hydrolysed fragment of this protein. 相似文献
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10.
P Musiani L Lauriola M Piantelli 《Clinica chimica acta; international journal of clinical chemistry》1978,85(1):61-66
Complexes between alpha-1-antitrypsin (alpha 1AT) and monoclonal IgA are regularly demonstrable in the plasma of myeloma patients. These alpha 1AT-IgA complexes, free of contamination by unbound alpha 1AT, are purified from 5 myeloma patients sera using salt-mediated hydrophobic chromatography. The complexes have a molecular weight greater than or equal to 400 000: this suggests that alpha 1AT is bound to di- or polymeric IgA. The alpha 1AT bound to IgA constitutes the 3.2, 3.5, 7.2, 8.5, and 24.6 per cent of the total alpha 1AT present in the 5 myeloma serum samples. There is a linear correlation between bound alpha 1AT concentration and IgA level in the range of the IgA concentrations considered (r = 0.988; p less than 0.05). Similar values are obtained quantitating bound alpha 1AT by radioimmunodiffusion technique or by determination of the trypsin-inhibiting capacity; this demonstrates that the bound alpha 1AT fully retains its inhibitory capacity. The biological significant of this binding phenomenon is discussed. 相似文献
11.
S Galvez A Farcas M Monari 《Clinica chimica acta; international journal of clinical chemistry》1979,91(2):191-196
Forty patients with histologically proved intracranial tumors were submitted to preoperative lumbar and venous punctures. The concentration of alpha-1-antitrypsin in the serum and in cerebrospinal fluid was determined by radial immunodiffusion technique. In the series, both serum and cerebrospinal fluid alpha-1-antitrypsin concentrations are significantly higher than the mean normal values. No statistical differences are observed in serum alpha-1-antitrypsin levels between benign and malignant tumors, but cerebrospinal fluid alpha-1-antitrypsin is significantly increased in the group of malignant tumors if cases undergoing steroid treatment are excluded. The diagnostic value of alpha-1-antitrypsin determinations in groups of patients with intracranial tumors is suggested. 相似文献
12.
Changes in the serum concentrations of thyroxine-binding prealbumin and retinol-binding protein following burn injury 总被引:1,自引:0,他引:1
Concentrations of thyroxine-binding prealbumin (TBPA) and retinol-binding protein (RBP) were measured by one-dimensional immunoelectrophoresis in sequential serum samples obtained from 16 patients suffering from burns of between 2 and 37% of the body surface area. The patients were assigned into two groups suffering from either moderate or mild burns on the basis of an age-related predictive index. In both groups the levels of TBPA and RBP fell with lowest levels occurring at 6 days post-burn. The time taken to return to normal levels was longer in the moderately burned group than in the mildly burned group. There was an association between the lowest levels reached and the severity of the burn as assessed by % burn area. This association was stronger when severity was assessed by the age-related predictive index. 相似文献
13.
R Sesboüé J P Basuyau J P Salier 《Clinica chimica acta; international journal of clinical chemistry》1983,135(1):23-33
Three blood protease inhibitors were immunochemically quantitated in normo- and hypertensive subjects divided according to their plasma renin activity. As inflammatory reactions could be suspected in many subjects, the inflammatory state was estimated on the basis of three acute phase reactants and allowed one to conclude that total inter-alpha-trypsin-inhibitor and inter-alpha-trypsin-inhibitor derivative, as well as alpha-1-antitrypsin levels were increased in inflammation. Involvement of either protease inhibitor tested in the control of plasma renin activity is unlikely as no relationship between plasma renin activity and protease inhibitor levels could be demonstrated in non-inflammatory conditions. Finally, there was no particular distribution of alpha-1-antitrypsin (PI) phenotypes in the overall population. 相似文献
14.
K Ohlsson U Akesson 《Clinica chimica acta; international journal of clinical chemistry》1976,73(2):285-291
Human granulocytes contain cationic proteins with chymotrypsin-like activity. These proteases showed a higher relative affinity for alpha1-antichymotrypsin than for alpha1-antitrypsin but the highest affinity for alpha2-macroglobulin. The complexes between cationic protein and alpha1-antichymotrypsin migrate as beta-globulin on agarose gel electrophoresis. 相似文献
15.
M Schwartz 《Clinica chimica acta; international journal of clinical chemistry》1982,124(2):213-223
4-methylumbelliferylguanidinobenzoate (MUGB) reactivity in plasma from patients with cystic fibrosis and in amniotic fluid from pregnancies leading to children with cystic fibrosis, has been reported to be significantly decreased. We have so far been unable to confirm these findings and have therefore reexamined this reactivity using diisopropylfluorophosphate (DEF), another active site titrant of serine proteases. We have shown that MUGB and DFP are reacting with the same molecules in plasma and amniotic fluid. Using crossed immunoelectrophoresis and SDS-polyacrylamide gel electrophoresis of 3H-DFP labelled plasma and amniotic fluid we have obtained strong evidence that the main contribution of MUGB and DFP reactive protein in plasma and amniotic fluid is identical to serum albumin. The use of MUGB reactivity in amniotic fluid in pregnancies at risk for cystic fibrosis must therefore be reconsidered. 相似文献
16.
P Schultz-Larsen J Lyngbye J G Westergaard B Teisner 《Clinica chimica acta; international journal of clinical chemistry》1979,99(1):59-69
A comparison has been made between rocket-immunoelectrophoresis (RIE), radial immunodiffusion (RID) and automated immunonephelometry (AIP) in the assay of pregnancy-specific beta 1-glycoprotein (SP1) in serum from pregnant women. Using RIE an interaction was demonstrated between the various SP1-reactive molecular populations causing a bias of up to 10%. An interaction corresponding to this phenomenon cannot be demonstrated when using RID and AIP. When correlating the serum-SP1 concentration of samples containing various ratios of SP1-reactive molecules by means of RIE, RID and AIP, it was demonstrated that there was no correlation between the results achieved using one method compared to the results achieved by either of the other methods. The results achieved using one method can therefore exclusively be judged from reference values determined using the same method. The analysis time is essentially shorter with AIP than with RIE and RID. 相似文献
17.
I R Brown N D Carter A Sood 《Clinica chimica acta; international journal of clinical chemistry》1979,95(1):75-82
The binding properties towards vitamin D metabolites of plasma from individuals with the three common Gc-globulin phenotypes, Gc-1, Gc-2 and Gc-2-1, have been found to be identical. In patients with liver disease there is a good correlation between the levels of Gc-globulin andalbumin in plasma. In addition the Gc-globulin levels correlate well with the ability of plasma to bind 25-hydroxycholecalciferol. Patients with the Gc-2-1 phenotype showed a significantly smaller depression in plasma Gc-globulin than those with the Gc-2 and Gc-1 phenotypes. The relation of these findings to the pathogenesis of disorders of calcium metabolism in liver disease is discussed. 相似文献
18.
F Anthony K Spencer P Mason G M Masson C P Price P J Wood 《Clinica chimica acta; international journal of clinical chemistry》1980,105(2):287-295
Four methods (radioimmunoassay, electroimmunoassay, laser nephelometry and kinetic immunoturbidimetry) have been evaluated for the measurement of proteins with pregnancy-specific beta1 glycoprotein immunoreactivity. The influence of two maternal plasma proteins with different electrophoretic mobilities (alpha2 and beta1) but with similar antigenic determinants has been assessed in each assay system. The radioimmunoassay method, which utilises a homogeneous preparation of pregnancy-specific beta1 glycoprotein for the preparation of iodinated tracer, and the kinetic immunoturbidimetric assay which measures antigen-antibody complex formation over the first minute of the reaction, were both considerably more specific for the beta1 component of pregnancy specific beta1 glycoprotein immunoreactivity than the laser nephelometric or electroimmunoassay methods. 相似文献
19.
N C Kar C M Pearson 《Clinica chimica acta; international journal of clinical chemistry》1976,73(2):293-297
Human skeletal muscle homogenate was found to degrade casein-yellow substrate slowly at neutral pH. This protease activity was maximally stimulated by 1 mM Ca2+. A significant increase in the level of Ca2+-activated neutral protease was found specifically in muscle of five patients with Duchenne dystrophy and one patient with the Becker form of X-linked dystrophy. 相似文献
20.
P Stromber D Ballantyne F C Ballantyne J L Third D K Bedford 《Clinica chimica acta; international journal of clinical chemistry》1977,79(1):163-172
The lipid and protein composition of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) subfractions (Sf greater than 100, 60--100 and 20--60 VLDL and Sf 10.4--20, 5.7--12 and 3.5--6.5 LDL) in six subjects with type III hyperlipoproteinaemia (HLP) was compared to that of 12 normal subjects. In type III HLP all VLDL subfractions contained increased concentrations of cholesterol and triglycerides and were relatively enriched in cholesterol. VLDL of Sf 20--60 also contained and increased concentration of B-protein. The tetramethylurea (TMU) soluble apolipoproteins of the VLDL subfractions were separated by polyacrylamide disc gel electrophoresis. In the subjects with type III HLP the proportion of arginine rich protein (ARP) was increased in all subfractions. The concentrations of cholesterol and triglycerides were increased in the LDL subfraction of Sf 10.4--20 and cholesterol was decreased in LDL of Sf 5.7--12, but the ratios of cholesterol to triglycerides were not significantly different from those in the LDL subfractions of the normal subjects and the protein composition was also similar. These results provide further evidence that in type III HLP abnormalities are not confined to the stage of conversion of VLDL to LDL, but occur throughout the VLDL spectrum. 相似文献