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1.
The appearance of joint inflammation (JI) 14 days after the injection of adjuvant (AJ) in the tail of rats is associated with a cachectic syndrome which is characterised by marked weight loss (WL). The degree of weight loss was examined in relation to the extent of change in other markers of inflammation including increased plasma copper (pCu), decreased plasma zinc (pZn) and increased hepatic metallothionein (hMT). At 14 days post-AJ injection, arthritic rats showed the following changes, relative to the controls: body weight, 12% decrease, pZn, 50% decrease; pCu, 90% increase and hMT, 11-fold increase (allp<0.001). Significant relationships were observed between JI, WL, pZn and hMT. The following coefficients of determination (r
2) were observed; JI and WL,–0.530, JI and pZn,–0.485; JI and hMT, 0.286; WL and hMT, –0.510 (allp<0.007). There was a strong relationship between the decreased pZn and increased hMT;r
2=–0.456 (p<0.001). While increased pCu was clearly associated with AJ-arthritis in these rats, there was no quantitative relationship between the extent of change in pCu and the other parameters measured (r
2 all<0.01). The highest correlation observed was between pZn and WL (r
2=0.637,p<0.001)., While the initial depression of pZn may be the result of increased hepatic hMT levels, longer term reductions in pZn levels are linked to systemic inflammation, the degree of arthritis and associated weight loss. 相似文献
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Rovenský J Svík K Matha V Istok R Ebringer L Ferencík M Stancíková M 《Clinical & developmental immunology》2004,11(3-4):267-273
The effects of probiotic bacteria Enterococcus faecium (EF) and selenium were studied on methotrexate (MTX) treatment in rats with adjuvant arthritis (AA). Arthritic rats were preventive treated orally with the following substances: lyophilized EF (15mg/kg/day, 5 days a week); sodium selenite pentahydrate (SSe, 0.050mg/kg containing 0.015 mg/kg selenium, 5 days a week); MTX (0.6 mg/kg/week), and their combinations for the period of 50 days from adjuvant application. Levels of serum albumin, serum nitrite/nitrate concentrations, hind paw swelling, arthrogram scores, whole body bone mineral density (BMD), and bone erosions were evaluated as markers of inflammation and destructive changes associated with arthritis. Long-term preventive treatment with low-dose MTX significantly inhibited the markers of both inflammation and arthritis. EF or SSe when administered singly or in combination had no significant effect on given parameters in arthritic rats. EF but not SSe potentiated the beneficial effects of MTX, which resulted in a more significant reduction of hind paw swelling, arthrogram scores and whole body BMD decrease. EF had a tendency to improve also the effect of MTX on serum albumin and nitrite/nitrate concentrations. Our results indicate that EF may increase the preventive effect of MTX treatment in rat AA by improving its anti-inflammatory and anti-arthritic effects. 相似文献
4.
Susceptibility to adjuvant-induced arthritis among germfree, specific-pathogen-free, and conventional rats. 下载免费PDF全文
O Kohashi J Kuwata K Umehara F Uemura T Takahashi A Ozawa 《Infection and immunity》1979,26(3):791-794
Germfree F344 rats developed severe arthritis with 100% incidence after a single intradermal inection of either squalane containing 0.5 mg of heat-killed Mycobacterium bovis BCG or a water-in-oil emulsion containing 0.2 mg of peptidoglycan derived from Staphylococcus epidermidis. Conventional F344 rats developed less-severe arthritis with 20% incidence for heat-killed BCG and 0% incidence for peptidoglycan. Specific-pathogen-free rats showed an intermediate susceptibility between germfree and conventional rats. Interestingly, both unimmunized specific-pathogen-free and conventional rats. but not unimmunized germfree rats, showed weak delayed-type hypersensitivity reactions to peptidoglycans derived from either S. epidermidis or Lactobacillus plantarum, suggesting that a bacterial flora may furnish a stimulus for induction of cell-mediated immunity to ubiquitous bacterial peptidoglycans. It is thus possible that although a bacterial flora is not necessary for development of adjuvant arthritis, it may have some suppressive effect on the development of the disease in specific-pathogen-free and conventional F344 rats, possibly through modulation of the immune response. 相似文献
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Alessandra Mileni Versuti Ritter Luzmarina Hernandes Bruno Ambrosio da Rocha Camila Fernanda Estevão-Silva Edirlene Sara Wisniewski-Rebecca Joice dos Santos Cezar Silvana Martins Caparroz-Assef Roberto Kenji Nakamura Cuman Ciomar Aparecida Bersani-Amado 《Inflammation research》2017,66(8):725-737
Aim
This study evaluated whether anethole attenuates the inflammatory response and joint damage in a model of adjuvant-induced arthritis (AIA) in rats.Methods
The animals were treated with 62.5-, 125-, or 250-mg/kg anethole daily for 21 days after AIA and necropsied on days 14 and 21 to evaluate the number of serum and synovial leukocytes (total and differential), serum cytokines (IL-2, IL-6, IL-12, IL-17, and TNF-α), and nitric oxide concentrations. Morphologic changes in the cartilage and bone of the femorotibial articulation in both left paw and right paw were studied in hematoxylin/eosin and Sirius Red-hematoxylin sections.Results
Different doses of anethole suppressed paw swelling and the number of serum and synovial leukocytes. However, 250 mg/kg of anethole more effectively controlled local and systemic inflammation. Histological evaluation revealed significant prevention of cartilage damage and inflammatory infiltrate scores. Morphometric analysis showed pannus formation, the thickness of the articular cartilage, and bone resorption lower in the anethole-treated AIA group compared to untreated AIA group on both days 14 and 21. These significant anti-inflammatory effects in the anethole-treated AIA group were associated with downregulation of cytokines and nitric oxide levels.Conclusion
Therefore, anethole may be a useful intervention to treat inflammatory arthritis.6.
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OBJECTIVE AND DESIGN: Anti-arthritic effect of FTY720, a novel immunosuppressant, was compared with those of immunosuppressants cyclosporin A and tacrolimus in adjuvant-induced arthritis in rats. MATERIAL: Male LEW rats. TREATMENT: FTY720 (0.03-0.3 mg/kg), cyclosporin A (1-10 mg/kg) or tacrolimus (0.3-3 mg/kg) were orally administered to rats for 21 days beginning on the day (day 0) of adjuvant inoculation. In addition, the anti-arthritic effect of FTY720 (0.3 mg/kg) and cyclosporin A (10 mg/kg) were evaluated by administration to animals for 5 consecutive days (days 2-6, 6-10, and 10-14). METHODS: Adjuvant-induced arthritis was produced by intradermal injection of 0.5 mg heat-killed Mycobacterium tuberculosis. Hindpaw edema was measured plethysmographically. The day of arthritis onset was determined macroscopically. Bone degradation was determined by radiography. Peripheral blood leukocytes were classified microscopically. RESULTS: All test compounds inhibited the incidence of arthritis, hindpaw edema and bone destruction. In addition, FTY720 but not cyclosporin A or tacrolimus markedly decreased the number of peripheral blood lymphocytes. FTY720 treatment on days 6 to 10 inhibited the bone destruction and hindpaw edema. CONCLUSION: These results suggest that the anti-arthritic effect of FTY720 in this adjuvant-induced arthritic model was more potent than those of cyclosporin A and tacrolimus. FTY720 administered on days 6 to 10 showed the inhibitory effect on the bone destruction and hindpaw edema. FTY720 may be effective in the treatment of rheumatoid arthritis. 相似文献
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Shi Ping ZHAO Jian Min LI Gui Xiang FU Yong ZHOU Institute of Clinical Medical Sciences China-Japan Friendship Hospital Beijing P. R. China Beijing University of Traditional Chinese Medicine Beijing P. R. China 《中华微生物学和免疫学杂志(英文版)》2006,(3)
The aim of this study was to explore the effect of Cornus officinalis glucosides (COG) on adjuvant-induced arthritis in rats and its mechanism. Seventy-two rats were divided into six groups of normal, model, Dexasone (0.125 mg/kg), high-dose COG (240 mg/kg), mid-dose COG (120 mg/kg), and low-dose COG (60 mg/kg). Rat arthritis was induced by injection of Freund's complete adjuvant in the hind paws. All treatment started from the day the arthritis was induced. The edema degree of the adjuvant injection location was determined on days 1, 3, 5, 7, 9, 11, 13, 15, 17, 20, 23 and the opposite side was observed on days 11, 13, 15, 17, 20, 23 after the injection of adjuvant. All rats were sacrificed on day 24 after the injection of adjuvant for microscopic examination of the ankle, and for the study of the immunological molecular mechanism. The results showed that the COG significantly suppressed both the primary and secondary edema, improved pathological injuries of adjuvant arthritis (AA) rat ankles, significantly suppressed the proliferation of T lymphocytes and DTH reaction. It significantly suppressed IL-1, IL-6 and TNF-αproduction from peritoneal macrophages and PGE2 in plasma. In conclusion, the Cornus officinalis glucosides (COG) is able to prevent and cure the rat adjuvant-induced arthritis, and can suppress the production of pro-inflammatory cytokine IL-1, IL-6, TNF-αand PGE2. 相似文献
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High-density proteoglycan induces specific suppression of adjuvant-induced arthritis in rats. 下载免费PDF全文
I Saikawa T Hotokebuchi H Miyahara T Tokito T Maeda C Arita Y Sugioka 《Clinical and experimental immunology》1994,95(3):424-429
We compared the levels of serum antibodies in male and female C57Bl/6 mice during the primary and after challenge infection with Giardia muris. Male mice began passing cysts in their faeces earlier than females, and were shedding cysts for over 60 days, while females stopped shedding cysts by day 20 after infection. In both males and females there were significant increases in parasite-specific IgM 10 and 20 days after infection. No differences in parasite-specific serum IgA were observed until 40 days after infection. Parasite-specific IgG (whole) levels were elevated on days 20 and 40 in females, while males showed no significant increases. In addition, females had a much stronger IgG2b and IgG3 response than males. After challenge with either cysts or soluble parasite protein only the females had significant increases in specific anti-parasite IgG2b. Our data show differential ability of males and females to control the infection with G. muris is paralleled by a difference in the anti-parasite serum IgG response of the mice. 相似文献
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Ni Zhang Hui Zhang Betty Yuen Kwan Law Ivo Ricardo De Seabra Rodrigues Dias Cong Ling Qiu Wu Zeng Hu Dan Pan Jin Yun Chen Yan Fu Bai Jing Lv Li Qun Qu Xi Chen Qi Huang Wei Zhang Li Jun Yang Lu Yu Yu Han Guo Xin Huang Hui Miao Wang Xiao Lei Sun Yun Zhang Hu Qiang He Wei Dan Luo Yao Xiao Jian Zhou Ting Xu Qing Chun Huang Min Wu Zhi Sheng Huang Wei Liu Vincent Kam Wai Wong Liang Liu 《Cellular & molecular immunology》2020,17(11):1190
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B. D. Jaffee J. S. Kerr E. A. Jones J. V. Giannaras M. McGowan N. R. Ackerman 《Inflammation research》1989,27(3-4):344-346
The purpose of this study was to investigate the effects of cyclosporine A (CSA) and methotrexate (MTX) as potential immunomodulators in a nonestablished adjuvant arthritis (AA) model. Non-injected hind paw volumes were reduced when AA rats were treated for 18 dayswith CSA (100% at 10 mg/kg) or MTX (100% at 0.1 mg/kg). Body weights of drug treated AA rats were increased above untreated AA rats and were similar to non-arthritic controls. AA rats show elevated T helper (W3/25+)/T suppressor (OX 8+) cell ratios (2.0 vs. 3.1,p<0.01). The immunomodulators tested all returned these elevated ratios to control non-arthritic levels. Similarly, these drugs returned the reduced mitogen responses and elevated blood granulocyte numbers toward normal non-arthritic control values. 相似文献
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James R. Bowen Glen R. Gale William A. Gardner Jr. Walter M. Bonner Jr. 《Inflammation research》1974,4(2):108-112
Cis-Dichlorodiamminepaltinum(II) (cis-PtII) suppressed adjuvant-induced arthritis in rats as assessed by paw volume, paw temperature, and microscopic appearance. Treated animals incurred lymphocytopenia and thymic atrophy. Paws from a number of rats with arthritis which were treated withcis-PtII showed focal bone marrow congestion and hemorrhages not found in rats with untreated arthritis. 相似文献
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P Chappuis J Arnaud M C Jaudon R Zawislak J Bellanger 《Annales de biologie clinique》1991,49(8):421-427
Total parenteral nutrition (TNP) therapy is widely used. However the quantitative requirements or the toxicity of trace elements in parenteral solutions are difficult to assess. This paper deals with a study performed by the Trace Element Commission of the Société Fran?aise de Biologie Clinique. Trace metals (zinc, copper, selenium and aluminium) which are mainly involved in TPN solutions are analyzed in 12 different parenteral nutrition solutions commercially available. This multicentric assay (5 different sites of analysis) shows that a slight pollution can be noted for nearly all the solutions examined. But at this level (10 mumol/l for the most concentrated solution), the zinc intake cannot induce any toxicity. For copper and selenium the results indicated a negligible pollution. Small-volume solutions added with zinc, copper and selenium are correctly supplemented. As regard aluminium pollution, 4 solutions among 12 contain non negligible amounts of aluminium. The consequences of this TPN overload especially for young infants, indicate that the struggle against this pollution has to be strengthened. 相似文献
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大鼠佐剂性关节炎模型表现特征及评价指标 总被引:3,自引:0,他引:3
目的:描述大鼠佐剂性关节炎(Adjuvant-induced arthritis,AA)临床表现主要指标的评价方法。方法:完全弗氏佐剂免疫SD大鼠诱导AA模型,进行全身及关节炎指数评分、计算关节肿胀数、测定足爪肿胀度、进行足爪X线摄片、踝关节病理检查及评分。结果:大鼠免疫后11天(d11)出现足爪红肿,全身及关节炎指数评分和关节肿胀数增加。炎症高峰期在免疫后d19~d28。大鼠出现活动障碍,可见耳朵、鼻和尾根部"关节炎"结节。X线可见关节软组织肿胀、骨密度降低、骨侵蚀病灶以及关节间隙狭窄甚至消失。踝关节病理见滑膜组织增生,炎细胞浸润,血管翳出现等。结论:大鼠AA是兼有局部关节炎症和全身表现的类风湿性关节炎动物模型。关节炎指数、关节肿胀数、足爪肿胀度、足爪X线摄片、踝关节病理等是评价AA模型临床表现的主要指标。 相似文献
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T. V. Davydova V. G. Fomina L. A. Basharova V. A. Evseev S. I. Igon'kina O. I. Mikovskaya 《Bulletin of experimental biology and medicine》1997,124(3):879-881
Active immunization with a serotonin—protein conjugate inducing the formation of antiserotonin antibodies exerts an analgesic
effect in rats with adjuvant-induced arthritis and inhibits the development of arthritis in early stages after injection of
Freund's complete adjuvant.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 124, No. 9, pp. 292–295, September, 1997 相似文献
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G B West 《International archives of allergy and applied immunology》1980,63(3):347-350
Rats fed a high carbohydrate or a high protein diet deficient in copper, magnesium or zinc, or in the three trace elements together, failed to develop adjuvant-induced arthritis, yet a deficiency of both copper and magnesium in the diets exhibited no such inhibition. On the other hand, only a magnesium deficiency reduced the severity of the dextran anaphylactoid reaction. 相似文献
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Huaxun Wu Jingyu Chen Qingtong Wang Xiaoyi Jia Shasha Song Pingfan Yuan 《Immunopharmacology and immunotoxicology》2014,36(2):124-129
Objective: To investigate the effects of ginsenoside metabolite compound K (CK) on adjuvant-induced arthritis (AA) rats and the partial mechanisms focused on the function of immunocyte (B cell and macrophage) and effectors’ cell (fibroblast-like synoviocyte, FLS).Methods: Animals were divided randomly into nine groups including control, AA, CK (5, 10, 20, 40, 80, and 160?mg/kg, i.g.), and MTX (0.5?mg/kg, i.g.). The effects of CK on AA rats are evaluated by swelling of the paw, histopathology of joint, and inflammatory cytokine production in serum. To further investigate the effects of CK on the function of B cell, peritoneal macrophage, and FLS from AA rats, we examined the proliferation of B cell and FLS by [3H] thymidine incorporation, and the phagocytic function of peritoneal macrophage was measured by neutral red uptake. Cytokines and antibodies in serum and the supernatant from peritoneal macrophage and FLS were measured by ELISA kit.Results: CK suppressed the severity of AA rats by attenuating the paw swelling and histopathology of joint. CK can inhibit the proliferation of B cell and autoantibody levels, and suppressed the phagocytic function of peritoneal macrophage and secreted pro-inflammatory cytokines TNF-α, IFN-γ, and IL-17 and up-regulated the level of protective cytokines IL-10. CK attenuated the proliferation of FLS, and balanced the ratio of RANKL to OPG in AA rats.Conclusion: Our results suggest that CK may attenuate the severity of AA rats, partially by influencing the function of immunocyte (B cell and macrophage) and effectors’ cells (FLS) in AA. 相似文献
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Haruta K Mori S Tamura N Sasaki A Nagamine M Yaguchi S Kamachi F Enami J Kobayashi S Yamori T Takasaki Y 《Inflammation research》2012,61(6):551-562