生物材料表面固定粘附肽调节小鼠成骨细胞骨钙素的表达

目的：观察材料表面固定粘附肽RGD对小鼠成骨细胞骨钙素mRNA表达的影响。方法：采用自组装单层结构法含RGD的粘附肽PGDC共价结合在试件表面,建立RDGC和空白对照组。接种小鼠原代成骨细胞,分别收集培养第10,15,21天的细胞,提取总RNA,Northen印迹杂交法检测骨钙素mRNA的表达。结果：RGD组第15天即开始出现明显的骨钙素基因表达,对照组的基因表达出现在第21天。结论：材料表面固定RGD有促进骨钙素基因表达,影响小鼠成骨细胞分化的功能。     

导电高分子聚吡咯纯钛表面改性对成骨细胞生长的影响

目的 :研究电场刺激下纯钛表面聚吡咯涂层对成骨细胞的黏附、增殖的影响。方法 :10 0mV阳极电刺激下 ,观察接种 4、8、12、16、2 0、2 4h成骨细胞的黏附数量以及 2 4h、72h的MTT吸光度值 ,SEM观察成骨细胞的生长情况。结果 :电刺激组 (Ti+电刺激 ,Ti+Ppy +电刺激 )的成骨细胞贴附密度在 4、8、12、16h均高于无电刺激组 (Ti、Ti+Ppy) (P <0 .0 5 ) ;聚吡咯涂层电刺激组的成骨细胞贴附密度 (Ti +Ppy +电刺激 )在4h、8h高于单纯电刺激组 (Ti+电刺激 ) (P <0 .0 5 ) ;72h后钛表面聚吡咯涂层加电刺激组 (Ti+Ppy +电刺激 )的吸光度高于其它三组 (Ti、Ti+Ppy、Ti+电刺激 ) (P <0 .0 1)。 结论 :聚吡咯涂层具有良好的生物相容性 ,并且在阳极电刺激下可以显著的促进成骨细胞的早期贴附和增殖     

钛表面不同处理对成骨细胞生长影响的实验研究

目的：探讨纯钛表面不同处理对成骨细胞生长的影响。方法：分离、切取日本大耳白兔胫骨骨膜,应用植块法培养兔骨膜原代成骨细胞,应用碱性磷酸酶（ALP）染色,钙结节染色,进行成骨细胞鉴定。将原代培养的成骨细胞与不同处理的纯钛片（抛光处理、喷砂处理）紫外灯光照处理后联合培养。采用扫描电镜、碱性磷酸酶活性（ALP）检测,MTT检测,观察不同处理表面的微型态对成骨细胞黏附、增殖的影响。结果：扫描电镜观察成骨细胞平铺在抛光处理的钛片的表面,没有伪足伸出;在喷砂处理的钛片表面上成骨细胞伪足伸入孔洞内,有伪足伸出。喷砂组ALP活性明显高于抛光组。结论：粗糙钛表面比光滑钛表面更有利于成骨细胞的黏附、增值;紫外灯光照钛片对成骨细胞的黏附、增殖无不利影响。     

纳米颗粒钛膜对成骨细胞合成功能的影响

目的通过对比不同纳米颗粒钛膜表面成骨细胞的合成能力,评价其生物相容性。方法采用直流磁控溅射法通过控制温度（常温、100 ℃、250 ℃、380 ℃）构建4级纳米颗粒钛膜,将SD乳鼠第3代成骨细胞接种于其表面及未镀膜的钛片表面,对表面成骨细胞上清液中骨钙素（OC）含量进行检测。结果在7、14 d时,随着时间的延长,各实验组和对照组样本表面的细胞上清液中OC含量均增大。在7 d时,对照组与其他各组相比,OC含量间差异均有统计学意义（P＜0.05）。在14 d时,100 ℃实验组表面OC含量增幅最大,100 ℃实验组与380 ℃实验组、对照组、空白组相比,其差异具有统计学意义（P＜0.05）,250 ℃实验组与380 ℃实验组、对照组、空白组相比,其差异也具有统计学意义（P＜0.05）。结论钛表面纳米改性有利于其生物相容性,不同纳米粒径可以影响成骨细胞的合成功能。     

Follow-up examinations after removal of titanium plates coated with anodic titanium oxide ceramic

Transformation of the titanium metal surface with titanium oxides produced in various ways belongs among the most up-to-date procedures. The authors as pioneers in this field (e.g. Nobel Biocare TiUnite surface), have been utilizing for more than 15 years dental root implants and fixing elements (for mandibular osteosynthesis) coated with titanium oxide ceramics, produced by anodic oxidation and thermal treatment. The aim of this work was to assess the extent to which a titanium oxide ceramic coating influences the fate of plates applied for osteosynthesis within the human body. During a 5-year period (1995-1999), 108 of 1396 titanium oxide ceramic plates had to be removed for various reasons: loosening of the plate [47], osteomyelitis [25], a palpable swelling and tenderness [21] at the request of the patient for psychological reasons (13) or breaking of the plate [2]. When these 108 plates were removed, it was not possible to detect metallosis in even a single case; nor was there any tissue damage that could be attributed to the surface of the plates, whereas the literature data indicate that such damage is relatively frequent in the environment of traditional titanium fixing elements. The present investigation confirms the favourable properties of the titanium oxide ceramic surface.     

Removal of titanium plates coated with anodic titanium oxide ceramic: retrospective study

Transformation of the surface of metallic titanium with titanium oxides prepared in various ways is a modern procedure. For more than 15 years, the authors have been utilizing fixing elements coated with titanium oxide ceramics, prepared by anodic oxidation and thermal treatment, for purposes of jawbone osteosynthesis. The aim of the authors' work was to assess the extent to which the titanium oxide ceramic coating influences the fate of the plates used for osteosynthesis within the human organism, in regard to the possible need for their removal. During a 5-year period, 108 of 1,396 plates coated with anodic titanium oxide had to be removed for various reasons: plate exposure (47), osteomyelitis (25), palpable swelling and tenderness (21), patient request for psychological reasons (13), or fracture of the plate (2). In none of these 108 cases was metallosis observed, which otherwise is reported relatively frequently in the vicinity of traditional titanium fixing elements, nor was any tissue damage connected with the surface of the plates. The results indicate the favorable properties of the titanium oxide ceramic surface.     

新型钛合金2种不同表面处理对成骨细胞早期附着的影响

目的研究新型低弹性模量钛合金表面及其微弧氧化及耐磨处理后的表面形貌及粗糙度变化,以及不同表面处理的成骨细胞附着与2种涂层的表面形貌变化对细胞早期动力学行为影响特征。方法通过扫描电子显微镜（SEM）观察未处理组不同表面的形貌; 采用SD新生鼠颅骨分离成骨细胞接种在材料表面并进行体外共同培养,MTT法检测30、60、120 min的不同表面的细胞早期附着量;细胞培养2 h后通过激光扫描共聚焦显微镜（CLSM）观察附着细胞的骨架蛋白结构。结果未处理组、耐磨与微弧氧化组材料表面的粗糙度值依次增加;细胞早期附着的吸光度A值随材料表面的粗糙度增加呈上升趋势,各组内3个时间点的细胞附着A值随时间延长而递增;2 h附着细胞骨架蛋白的量在微弧氧化、耐磨处理组表面表达均优于未处理组。结论2种不同涂层处理后新合金表面的形貌及粗糙度发生改变,进而影响到细胞在其表面的早期附着的量及其形态、骨架蛋白的表达。     

RGD肽修饰的纯钛种植材料表面成骨细胞黏附增殖能力

目的:评价精氨酸-甘氨酸-天冬氨酸(RGD)肽修饰纯钛表面对细胞早期黏附增殖影响. 方法:利用分子自组装技术氨基化纯钛表面,化学接枝GYRGDS肽,计算不同时点材料表面黏附细胞数;不同浓度RGD肽液预处理接种前细胞,计算材料表面细胞黏附率;MTT法和扫描电镜分别检测材料表面细胞的增殖、生长.结果:RGD肽修饰的纯钛表面细胞早期黏附率、增殖活性和生长情况在各时点均优于未修饰组,高浓度肽液预处理对RGD肽修饰组表面细胞有较强黏附抑制作用.结论:采用分子自组装技术可将活性RGD肽稳定的化学接枝于纯钛表面,修饰后材料的细胞早期黏附增殖等生物学行为有明显改善.     

钛片表面粗糙度和氧化膜对成骨细胞黏附影响的体外研究

目的 研究钛片表面粗糙度和氧化膜对成骨细胞黏附的影响,为选择临床种植体的表面处理方法提供依据.方法 250片纯钛钛片分为5组.采用直径分别为108～130 μm(S<,1>组)、216～301 μm(S<,2>组)、356～411 μm(S<,3>组)TiO<,2>砂对钛片表面进行喷砂处理:另外一组钛片采用钛浆喷涂(T...     

口腔正畸用磁块镀氮化钛膜后抗人工唾液腐蚀研究

目的：探讨正畸用磁块表面镀氮化钛膜后抗人工唾液腐蚀性能。方法：将镀膜、未镀膜磁块分别置入人工唾液中，37℃恒温下浸泡1个月，肉眼、EPMA观察2组磁块表面形貌特征。结果：镀氮化钛膜磁块表面均匀致密，无明显裂隙和崩解；而未镀膜磁块表面粗糙不平，有明显裂隙和崩解现象。结论：正畸用磁块表面镀氮化钛膜可大大提高磁块的抗腐蚀性能。     

Genetic effects of a titanium surface on osteoblasts: a meta-analysis

Titanium is used worldwide to make osseointegrable devices. The favorable characteristics that make this material desirable for implantation are i) its mechanical properties and ii) its biocompatibility. The latter has been demonstrated by in vivo studies with animal models and clinical trials over a forty-year period. However, the exact genetic effect of the titanium layer on cells is still not well characterized. To detect the genetic effect of titanium surfacing on osteoblasts without porosity as a confounding variable, a meta-analysis of genetic expression profiling obtained from several titanium surfaces was performed. One hundred seventy-three genes were selected, 84 up-regulated and 95 down-regulated. Among them, the most notable were genes that participate in osteoblast differentiation and bone regeneration, such as TIMP1, PTN, and RUNX1. Titanium surfacing has i) a direct genetic effect on osteoblasts, ii) induces several bone-related genes, and iii) is relevant to the osseointegration process. These properties should be taken into consideration in separating the effect of porosity (i.e. the micro-dimension) from implant coating (i.e. the nano-dimension).     

亲水性纯钛表面对成骨细胞黏附行为的影响

目的:体外研究亲水性喷砂酸蚀钛表面对成骨细胞黏附、铺展行为和黏着斑激酶(FAK)表达的影响.方法:钛片表面分别采用大颗粒喷砂酸蚀表面处理(sandblasted,large-grit,acid-etched,SLA)及亲水性化学活化大颗粒喷砂酸蚀表面处理(chemically-modified hydrophilic SLA,modSLA),在其表面接种人成骨细胞,对细胞黏附率、细胞铺展情况以及黏着斑激酶(FAK)的表达进行检测.应用SAS6.0软件包对数据进行统计学分析.结果:成骨细胞在modSLA表面的早期黏附率(1h、3h)显著高于SLA表面(P<0.05);接种3h后,modSLA表面的成骨细胞呈现更多的肌动蛋白结构和明显的成骨细胞骨架结构,细胞铺展更加明显,modSLA组细胞形状因子均值显著低于SLA组(P<0.05);免疫荧光分析显示,6h modSLA表面细胞内FAK的荧光强度高于SLA组(P<0.05).结论:亲水性化学活化大颗粒喷砂酸蚀处理钛表面较大颗粒喷砂酸蚀处理钛表面能显著增强成骨细胞在材料表面的贴附,促进细胞骨架沿一定方向伸展,促进黏着斑激酶(FAK)的表达,从而增强细胞的黏附力.     

两种钛表面成骨细胞附着静态与动态观察

目的体外构建钛表面成骨细胞生长模型,对细胞生长情况进行动静态观察。方法将光滑钛片（光滑钛片组）及多孔海绵状钛（海绵钛组）处理为酸碱表面和钙磷沉积表面,接种成骨细胞,12h、48h实时观察,48h后钛表面细胞固定,扫描电镜观察。结果光滑钛片组可见细胞充分延展附着,其中钙磷表面组细胞附着更多,可见细胞下钙磷沉积。海绵钛组可动态观察成骨细胞在海绵钛内立体生长,在钛珠问形成细胞桥。结论本研究构建形成钛表面成骨细胞生长体外模型,可进行钛表面细胞生长的实时观察与分析。     

多孔海绵状纯钛种植材料对成骨细胞生长的影响

目的：探讨具有连续多孔立体结构纯钛种植材料对成骨细胞生长的影响。方法：在体外对3种不同孔隙直径的多孔钛材料进行成骨细胞复合培养,在相差倒置显微镜下观察细胞在材料表面及孔隙中的生长情况,利用MTT法评价不同孔隙直径对细胞生长的影响。结果：成骨细胞在试件表面生长良好,成功长入连续孔隙之中,并以多突触的方式生长,在钛珠之间形成细胞桥;细胞增殖度检测显示多孔立体结构对成骨细胞的生长有一定的促进作用,200μTI孔径的作用最明显。结论：多孔立体结构能促进成骨细胞在纯钛种植材料表面和孔隙中的生长。     

Oxidized titanium screws coated with calcium ions and their performance in rabbit bone

PURPOSE: The aim was to answer a fundamental question: Do the chemical properties of titanium implants influence osseointegration? MATERIALS AND METHODS: Screw-type implants produced of turned commercially pure (grade 1) titanium (controls) and electrochemically calcium-deposited titanium implants (Ca test implants) were placed in the tibiae and femora of a total of 10 mature New Zealand white rabbits. The macro arc oxidation method was applied for Ca implants. Surface oxides were characterized with different analytic techniques, including x-ray photoelectron spectroscopy, auger electron spectroscopy, scanning electron microscopy, thin-film x-ray diffractometry, and TopScan 3D. The bone response was evaluated by biomechanical tests, histology, and histomorphometry. RESULTS: After a follow-up period of 6 weeks, test Ca implants showed a significant increase in mean peak removal torque (P = .0001) and in the histomorphometric measurement of bone-to-metal contact around the implants (P = .028) in comparison to controls. In addition, more mature mineralized bone was observed adjacent to test Ca implants compared to controls, as evaluated on 10-microm undecalcified, toluidine blue-stained, cut, and ground sections. DISCUSSION: The potential role of surface Ca chemistry to a superior bone response is discussed with specific reference to interaction with Ca(+)-binding proteins and function as binding sites of calcium phosphate mineral. CONCLUSION: The present results suggest that the surface chemical composition of titanium implants is of great importance for the bone response. Ca ion-deposited titanium implants showed fast and strong osseointegration in the rabbit bone model.