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1.
V A Isachenko S S Iamnikova L Ia Zakstel'skaia T A Grigor'eva O N Berezina 《Voprosy virusologii》1980,(1):49-53
The interrelations between H3/73 hemagglutinin of human influenza virus and the other 16 mammalian and avian hemagglutinin subtypes (a total of 50 strains) were studied by the method of radioimmunologic analysis (RIA). The antigenic relations of H3, Hav7 and Heq2 were confirmed, certain common determinants were also found in H3/73 hemagglutinin and avian viral Hav6 and Hav9 hemagglutinins. No interrelations were revealed with previously circulating human influenza viruses H0, H1, H2 as well as with swine influenza virus and avian viruses Hav1-Hav5, Hav8. It has been shown that the H3/73 determinant in some avian viruses evolves similarly to drift-variants of human influenza virus. The method can be recommended for fine analysis of influenza virus antigenic structure as it allows detecting small antigenic quantities. 相似文献
2.
K. Nerome M. Nakayama M. Ishida H. Fukumi W. K. Butterfield R. G. Webster C. H. Campbell 《Archives of virology》1978,57(3):261-270
Summary Twenty-two strains of influenza A virus were isolated from caged birds which had been imported into Japan from India and Thailand and had died during transportation to Tokyo.Serological tests divided these strains into two groups. Viruses in the first group contained Hav7 hemagglutinin and were related antigenically to A/duck/ Ukraine/1/63 [Hav7 Neq2]; viruses in the second group contained Hav4 hemagglutinin and were related to A/duck/Czech/56 (Hav4 Nav1]. All strains contained Neq2 neuraminidase that was closely related to that of A/equine/Miami/1/63 [Heq2 Neq2] and A/duck/Ukraine/1/63 [Hav7 Neq2]. It was concluded that the strains in the first group were Hav7 Neq2 and those in the second group were Hav4 Neq2; both groups of viruses showed antigenic drift from the prototype strains.With 2 Figures 相似文献
3.
Twenty-six influenza A viruses were isolated from cloacal and tracheal samples of 235 resident and 396 migratory ducks in Miyagi prefecture, Japan, in 1977--78. Of these, twelve were antigenically related to the avian-origin HSW1 virus, A/duck/Alberta/35/76 (HSW1N1), but their neuraminidase antigens were characterized as Nav2-3, Nav4 or N2. These antigenic configuration have not previously been reported. In addition, one strain in which the neuraminidase antigen was identified as Nav4, was demonstrated to be a mixture of two haemagglutinins, HSW1 and Hav7. Two distinct strains were separated from the mixture and characterized as HSW1Nav4 and Hav7Nav4. The antigenic identification of an additional 13 influenza A viruses revealed the presence of six haemagglutinin subtypes (Hav1, Hav3, Hav4, Hav6, Hav7, and Hav8) and five neuaraminidase subtypes (Nav1, Nav2-3, Nav4, Neq2, and N2) in various combinations. The results suggest that the avian influenza A viruses among feral ducks may be isolated in various combinations of haemagglutinins and neuraminidase subtypes in Japan, and that feral ducks may be the site of genetic recombination occurring as a result of dual infection with different subtypes of influenza A virus. 相似文献
4.
Ortho- and parainfluenza viruses isolated from the cloacas of migrating feral ducks shot on the Mississippi flyway included three strains of influenza. A virus (Hav6 Nav1, Hav6 Nl, Hav7 Neq2) as well as Newcastle disease virus. One influenza virus, A/duck/Memphis/546/74, possessed Hav3 haemagglutinin, but the neuraminidase was not inhibited by any of the known influenza reference antisera. The neuraminidase on this virus was related to the neuraminidases on A/duck/GDR/72 (H2 N?), A/turkey/Ontario/7732/66 (Hav 5 N?), A/duck/Ukraine/1/60 (Hav3 N?) and A/turkey/Wisconsin/68. We therefore propose that the neuraminidase on this group of influenza viruses be designated Nav6. The A/duck/Memphis/546/74 influenza virus caused an ocular discharge in 1 of 5 ducks and was shed in faeces for 10 days; it was stable in faecal samples for up to 3 days at 20 degrees C. These results suggest that ecological studies on influenza in avian species should include attempts to isolate virus from faeces. Faecal-oral transmission is an attractive explanation for the spread of influenza virus from feral birds to other animals. 相似文献
5.
Influenza A virus M protein was prepared by electrophoresis in SDS polyacrylamide gel from virus particles which had been pretreated with octylglucoside to remove the surface glycoproteins. M antigens from the influenza virus strains A/Victoria/3/75 (H3N2), A/FPV/Rostock (Hav1N1) and A/chick/ Germany/49 (Hav2Neq1) did not protect mice against a lethal challenge infection with the virulent Victoria strain.Supported by the Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 47) 相似文献
6.
Eight influenza virus A strains were isolated from 269 cloacal swabs taken from wild ducks (Anas platyrhynchos), shot during their autumn migrations in the years 1978-1981. One strain was identified as subtype A-H3N8N6 (Hav7Neq2Nav1), the remaining seven as subtype A-H4N6 (Hav4Nav1). 相似文献
7.
Summary Avian influenza A virus Hav 7 N 2 was transmitted to mink by contact. Other avian influenza A viruses, Hav 4 Nav 1 and Hav 6 Nav 5, were not transmitted, and human, swine and equine influenza A viruses were transmitted to mink by a similar contact. 相似文献
8.
Intestinal influenza: replication and characterization of influenza viruses in ducks. 总被引:43,自引:0,他引:43
Influenza A viruses isolated from the cloaca of naturally infected feral ducks replicate in the lungs and in the cells lining the intestinal tract of feral and domestic ducks. Despite the low pH of the gizzard, the duck influenza viruses reach the intestines via the digestive tract and are found in high concentration in the feces. The viruses retain infectivity in fecal material for at least 30 days at 4° and for 7 days at 20°. The morphology of one strain of intestinal duck influenza virus (Hav7 Neg2) that had never been passed in embryonated eggs and was isolated from the feces was roughly spherical and fairly uniform in size and shape. However, another strain of duck influenza virus studied (Hav3 Nav6) was predominantly filamentous, suggesting that the morphology of influenza viruses in their natural hosts varies from strain to strain. After passage in the chick embryo each strain retained the morphological characteristics found in the feces. In contrast to duck influenza viruses, representative human influenza viruses of the HON1, H3N2, and Hswl Nl subtypes replicate only in the upper respiratory tract of ducks. The duck influenza viruses are more stable to low pH than human strains and retain infectivity for over 30 days in nonchlorinated river water at 0° and for 4 days at 22°. The susceptibility of ducks to infection with human and avian strains of influenza virus and the possibility of transmission to animal species through the water supply suggests that ducks may be important in the ecology of influenza viruses. The possibility of “intestinal influenza” virus vaccines is considered. 相似文献
9.
Host range recombinants of fowl plague (influenza A) virus 总被引:9,自引:0,他引:9
Recombinants between the influenza virus strains fowl plague virus (FPV, Hav1N1) and Hong Kong (H3N2) have been isolated which form plaques on MDCK cells but not on chick embryo cells, although they carry the hemagglutinin of FPV. These host range recombinants have been characterized and one of them has been used for a second recombination with virus N (Hav2Neq1) or equi 2 (Heq2Neq2) on chick embryo cells. In this way, recombinants were obtained with a mixed genome which have regained the natural host range of FPV and some pathogenic properties for chicken. The results are discussed as a possible mechanism for a pandemic influenza strain to survive in an animal reservoir by changing its host range by recombination, and to regain the original host range by a second recombination but always keeping the same hemagglutinin. 相似文献
10.
Isolation of an influenza A virus from seals 总被引:11,自引:0,他引:11
Summary Influenza A virus of serotype Hav1 Neq1 (H7N7 by the 1980 revised influenza typing system proposed by WHO experts) was repeatedly isolated from lung and brain tissues taken from harbor seals (Phoca vitulina) found suffering from pneumonia on Cape Cod Peninsula (U.S.A.) in the winter of 1979–1980. The seal isolates, although of a serotype identical to some fowl plague virus strains, were harmless to chickens and turkeys in transmission experiments. An earlier human infection by a Hav1 Neq1 influenza virus and the serologic relatedness of this avian serotype with the equine 1 serotype are cited in support of the view that influenza viruses with these antigenic characteristics seem to have a facility to pass from birds to mammals. 相似文献
11.
Recombination between fowl plague virus FPV/Rostock (Hav1N1), an influenza A strain which produces a generalized fatal infection with involvement of the central nervous system in chickens, and mouse lung-adapted human strains England/1/61 (H2N2) or PR/8/34 (H0N1 results in recombinants which produce a generalized infection in suckling mice with involvement of the central nervous system. A combination of the hemagglutinin and M protein from FPV/Rostock (Hav1N1) with certain polymerase proteins from the mouse lung-adapted strains produces generalized infections in mice. Infectious virus was present in lung, brain, and blood even after intranasal infection. A correlation between the ability to induce a generalized infection of suckling mice in vivo and the replication in mouse embryo fibroblasts in vitro could be demonstrated. 相似文献
12.
E I Isaeva T S Belkina Z I Rovnova P N Kosiakov Ia M Selivanov 《Voprosy virusologii》1982,27(6):681-686
Comparative studies of the antigenic properties of hemagglutinin (HA) of animal and human viruses revealed both similarities between them and complete differences in the composition of antigenic determinants. Avian influenza viruses A/chicken/Kamchatka/12/71, A/pintail/Primorie/730/76, and A/bat/Alma-Ata/73/77 were completely identical with human strains of influenza virus. Influenza A/horse/Miami/63 contains one antigenic determinant H3.1.HA of A/tern/Turkmenia/18/73 (Hav7) viruses has a peculiar set of antigens. Apart from two antigenic determinants H3.1 and H3.3 inherent in human virus strains, HA of A/tern/Turkmenia/18/73 virus contains an antigenic determinant the population of antibodies to which shows no relation to HA of subtypes Hav2-Hav9. 相似文献
13.
Investigation of respiratory disease and high mortality which occurred on a commercial duck fattening farm between August 1979 and March 1980 resulted in the isolation of 10 influenza A viruses. The viruses were characterised as Hav6 N2 (three isolates), Hav4 Navl (four isolates), Hav4 Nl (two isolates) and Hav7 Neq2 (one isolate) subtypes by haemagglutination-inhibition and neuraminidase-inhibition tests. A Newcastle disease virus isolate was also obtained from the ducks. All isolates had low intravenous pathogenicity indices in 6-week-old chickens. 相似文献
14.
Two strains of influenza virus isolated from horses and ducks in 1963, A/equine/Miami/1/63 (Heq2 Neq2) and A/duck/Ukraine/1/63 (Hav7 Neq2) were found to possess hemagglutinin subunits which cross-reacted in hemagglutination-inhibition and immunodiffusion tests with those of the Hong Kong strain of human influenza A/Hong Kong/1/68 (H3 N2).Peptide maps of the heavy polypeptide chains from the hemagglutinin subunits of these three strains showed a number of differences, but maps of the light chains were almost identical, indicating that the light polypeptide chains from the hemagglutinin subunits of these animal, avian and human viruses had practically the same amino acid sequence.One explanation of these results is that the three viruses arose, by genetic recombination, from a common ancestor. 相似文献
15.
The structure of the hemagglutinin, a determinant for the pathogenicity of influenza viruses. 总被引:30,自引:0,他引:30
Comparative studies on naturally occurring avian influenza viruses have been carried out in order to investigate the determinant(s) for pathogenicity for chickens. At least one virus isolate from each of the nine different hemagglutinin (HA) subtypes was included. The polypeptides of these viruses were studied by analyzing infected cell extracts on SDS-polyacrylamide gels. Both viral glycoproteins, HA and neuraminidase, showed remarkable variation in their electrophoretic mobility even among serologically closely related viruses. Pulse-chase experiments revealed that most avian influenza virus strains had an HA which was not susceptible to proteolytic cleavage in MDCK, turkey (TEC), and chicken embryo cells (CEC). Only viruses belonging to the subtype Hav5 and some strains of the subtype Hav1 possessed a cleaved HA in these cells. Only the virus strains with cleaved HA were produced in infectious form in MDCK, CEC, TEC, as well as in duck embryo cells (DEC) and quail embryo cells (QEC). The other virus strains produced plaques in these cells only in the presence of trypsin. There was a strict correlation between the cleavability of the HA, the potential of the virus to be produced in infectious form in a wide range of host cells, and their pathogenicity for chickens. No evidence was obtained for an involvement of the neuraminidase in determining pathogenicity. For the nonpathogenic viruses it could be shown that they can replicate and produce infectious progeny in some organs of the chicken. The results obtained permit the conclusion that in naturally occurring avian influenza viruses the structure of the hemagglutinin, that is its susceptibility to proteolytic cleavage in a broad spectrum of host cells, is the determining factor for pathogenicity. 相似文献
16.
During 1973-1979 a marked increase in respiratory disease was seen in turkeys in the province of Verona in north east Italy. The disease was associated with virus isolations and serological evidence of influenza A infection. Seven of the influenza A viruses isolated from affected turkeys were of the Hav6 N2 antigenic subtype and one of the Hav2 N2 subtype. Pathogenicity index tests were done for seven of the virus isolates and indicated they were of low virulence. 相似文献
17.
Novel influenza A viruses isolated from Canadian feral ducks: including strains antigenically related to swine influenza (Hsw1N1) viruses. 总被引:10,自引:0,他引:10
Twelve influenza A viruses, antigenically related to the Ho, H1 and Hsw1 subtypes, were isolated from cloacal samples of feral ducks in Canada. Antigenic comparisons showed that these viruses were most closely related to the recent HSW1N1 isolates from man and pigs, whereas in vivo pathogenicity tests revealed differences between the Hsw1N1 viruses from the ducks and those from humans and pigs. Antigenic characterization of 94 additional influenza A viruses from the ducks showed four haemagglutinin subtypes (Hav1, Hav4, Hav5 and Hav7), an unclassified haemagglutinin, and six neuraminidase subtypes (N1, N2, Neq2, Nav1, Nav2 and Nav5) in various combinations, some of which are novel and have not previously been reported. Three of these duck influenza viruses possessed a haemagglutinin antigenically related to that of classical fowl plaque virus. A much higher percentage of virus isolations were from juvenile ducks (18.5%) than from adults (5%). All of the ducks, from which viruses were isolated, appeared healthy at the time of sampling. Serological studies on a limited number of humans and domestic birds living in close proximity to the Canadian ducks revealed no evidence of interspecies transmission. Our findings suggest that these birds serve as a substantial reservoir of antigenically diverse influenza viruses, including isolates antigenically related to the current human and animal influenza viruses. This reservoir in nature may be perpetuated by a cycle involving annual infection of juvenile birds followed by transmission to the remaining susceptible birds until the next congregation during the breeding season. 相似文献
18.
Characterisation of influenza A viruses isolated from turkeys in England during March-May 1979 总被引:1,自引:0,他引:1
During the early spring of 1979 turkeys on at least twelve sites in England became infected with influenza A viruses. On five of these sites no virus was isolated but birds were shown to have antibodies to Havl (four sites) and Hav2 antigenic subtypes of influenza A viruses. The eight viruses isolated were typed: A/turkey/England/192-328/79 (Havl Nav2/3), A/turkey/England/192-329/79 (Hav1 N2), A/turkey/England/199/79 (Hav1 Neq1), A/turkey/ England/214/79 (Hav1 Neq1), A/turkey/England/250/79 (Hsw1 N1), A/turkey/England/262/79 (Hav1 Nav2/3), A/turkey/England/272/79 (Havl Neq1), A/turkey/England/384/79 (Hav2 Nav4). Pathogenicity index tests in 6-week-old chickens agreed with the clinical signs seen in turkeys in the field. Three of the isolates: 199, 214 and 272 were of extremely high virulence, 384 showed intermediate virulence, while the other isolates were of low virulence. 相似文献
19.
R.G. Webster V.S. Hinshaw W.J. Bean K.L. Van Wyke J.R. Geraci D.J. St. Aubin G. Petursson 《Virology》1981,113(2):712-724
An influenza A virus antigenically similar to A/FPV/Dutch/27 (Hav1Neq1) [H7N7] was isolated from harbor seals (Phoca vitulina) that had died of acute hemorrhagic pneumonia on Cape Cod Peninsula, beginning in the winter of 1979–1980. High titers of virus were obtained from the lungs and lower titers from the brains of the seals. Although antigenic analyses and characterization of the RNAs show that all of the genes and gene products are closely related to different avian influenza viruses, biologically the virus behaves more like a mammalian strain. The seal virus replicated and produced pneumonia in experimentally infected harbor seals, but the clinical course and pathology were less severe than in the natural infection; the virus also replicated in ferrets, cats, and pigs but produced no disease. In avian species, the seal influenza virus replicated poorly, produced no disease signs, and was not shed in the feces. Although the seal influenza virus can cause conjunctivitis in humans who have known contamination of the eyes from infected animals, serological studies detected no evidence of seroconversion among persons working with infected seals or with the virus. Preliminary studies detected antibodies to this virus in harbor seals on the New England coast but not in harbor seals, gray seals, or fur seals from other areas, suggesting that this virus may be a new introduction to this species. An Hav1Neq1 [H7N7] virus was also isolated from feral ducks in Iceland in 1980, but the two viruses could be distinguished by analysis of their RNAs and host range. The A/Seal/Mass/1/80 influenza virus provides the first evidence suggesting that a strain deriving all of its genes from one or more avian influenza viruses can be associated with severe disease in a mammalian population in nature. Whether this breach of species specificity represents a unique event in influenza evolution remains to be determined, but raises the possibility that human or animal influenza viruses may be derived directly from avian strains. 相似文献
20.
We have found that hemagglutinin molecules from equine-2 (Heq2Neq2) and duck/Ukraine (Hav7Neq2) influenza viruses do not possess N-terminal aspartic acid and that the N-terminus of HA1 from these viruses seems to be blocked. In this respect, these hemagglutinin molecules are similar to those of Hong Kong influenza virus (H3N2) and its variants and unlike those of every other influenza virus examined (a total of 12 strains, including those of the Asian H2N2 series) which have been found to possess N-terminal aspartic acid (or asparagine) on the hemagglutinin polypeptides. 相似文献