~（14）C─尿素呼吸试验诊断幽门螺杆菌感染

幽门螺杆菌感染是消化性溃疡和胃炎的主要病因之一。１４Ｃ－尿素呼吸试验是诊断幽门螺杆菌感染的非侵入性方法，敏感性达９０％～９７％，特异性为８９％～１００％，主要适用于幽门螺杆菌感染患者抗菌性根治过程中的随访观察、评价疗效和确定进一步的治疗方案     

研究动机性访谈对幽门螺杆菌感染胃炎患者自我管理能力的干预效果

目的：研究动机性访谈对幽门螺杆菌感染胃炎患者自我管理能力的干预效果。方法采用便利抽样的方法抽取就诊的幽门螺杆菌感染胃炎患者156名，按照随机数字表法随机分为对照组和观察组，每组患者78例。对照组采用常规的护理方法，观察组采用动机性访谈的方法。对比干预后两组患者自我管理能力。结果除食物选择的自我管理能力外，其余的自我管理能力，两组具有显著性差异。结论作为一种以改变患者动机的干预方法，动机性访谈应推广到幽门螺杆菌感染胃炎患者的临床护理工作中去。     

幽门螺杆菌分泌与重组表达的VacA蛋白的分离纯化及其致细胞空泡效应与凋亡的对比研究

目的：分离纯化幽门螺杆菌分泌和重组表达的细胞空泡毒素抗原（ VacA）蛋白,并评价其致细胞空泡效应及致细胞凋亡效应。方法分别从幽门螺杆菌ATCC26695菌株培养上清和重组表达VacA蛋白的pQE30-VacA-E．coliM15基因工程菌中分离纯化VacA蛋白,经酸化后,以不同终浓度（5,10 ng／ml）分别与人胃腺癌AGS细胞共孵24 h,观察致空泡效应,并通过流式细胞术检测细胞凋亡。结果成功分离纯化出幽门螺杆菌分泌和重组表达的VacA蛋白;幽门螺杆菌分泌的VacA蛋白能显著引起AGS细胞的空泡样改变及凋亡（P＜0．01）,而重组表达的VacA蛋白致细胞空泡样改变及凋亡不显著（ P＞0．05）。结论幽门螺杆菌分泌的VacA蛋白有良好的空泡毒性及致凋亡效应,而重组表达的VacA蛋白无致空泡及凋亡效应,幽门螺杆菌分泌的VacA蛋白可用于VacA作用机制的研究。     

淋巴滤泡增生在幽门螺杆菌性胃炎中的病理诊断价值

目的 评价胃窦黏膜淋巴滤泡增生在幽门螺杆菌性胃炎病理诊断中的价值.方法 以淋巴滤泡增生为诊断幽门螺杆菌性胃炎的标准,与诊断幽门螺杆菌阳性的＂金标准＂进行临床流行病学分析.结果 在1385例慢性胃炎中,以淋巴滤泡增生为诊断幽门螺杆菌性胃炎标准,其诊断的准确度为79.35%,灵敏度为79.43%,特异度为79.18%,阳性预告值89.22%,阳性似然比3.93,漏诊率为20.57%,误诊率为20.82%,阴性预告值79.18%,阴性似然比0.26.结论 淋巴滤泡增生是幽门螺杆菌性胃炎的组织病理形态学特征之一,是诊断幽门螺杆菌性胃炎的重要组织学依据,但不是唯一标准.     

表观遗传调控对幽门螺杆菌阳性胃癌致病作用的研究进展

幽门螺杆菌(Hp)是一种常见的致癌因素,与胃癌的发病密切相关。幽门螺杆菌通过多种机制影响宿主基因的表达,包括表观遗传调控。随着对表观遗传学的深入研究,它与Hp阳性胃癌之间相互作用的证据也逐渐增多,靶向表观遗传学的改变有望成为Hp阳性胃癌的一种治疗策略。因此,该文对表观遗传调控在Hp阳性胃癌致病机制的影响及相互作用作一简要综述。     

248例海员胃及十二指肠疾病幽门螺杆菌感染调查

２４８例海员胃及十二指肠疾病幽门螺杆菌感染调查王新华自１９８３年Ｗａｒｅｎ和Ｍａｖｓｓｈａｌ从胃窦粘膜活检标本中分离出幽门螺杆菌（ｈｅｌｉｃｏｂａｃｔｅｒｉｕｍｐｙｌｏｒｕｓ，ＨＰ）之后，许多研究报告都证明，胃幽门螺杆菌是慢性胃炎的病因之一，且与消化...     

浅析幽门螺杆菌的临床治疗

正幽门螺杆菌(H,pylori)感染是慢性胃炎、消化性溃疡、胃黏膜相关淋巴组织淋巴瘤以及胃癌的主要病因,也是上述疾病不易愈合及易复发的主要因素,根除幽门螺杆菌对治疗上述疾病的作用不可忽视。幽门螺杆菌是一种革兰氏阴性微需氧杆菌,呈螺旋形,在胃小凹上部胃上皮表面和粘膜层中最易     

幽门螺杆菌HpaA减毒鼠伤寒沙门菌核酸疫苗的构建及免疫原性检测

目的 构建含幽门螺杆菌(H．pylori)hpaA基因的重组减毒鼠伤寒沙门菌核酸疫苗。方法 应用PCR方法从H．pylori标准菌株基因组DNA中扩增hpaA基因,克隆入pUCmT载体,检测hpaA基因序列,经过酶切、连接反应将其克隆入真核表达载体pIRES,转入大肠杆菌,筛选阳性克隆,通过PCR和酶切反应鉴定。重组载体pIRESrhpaA转化入减毒鼠伤寒沙门菌LBS000,抽提质粒,再转化入SL7207,反复传代,鉴定重组核酸疫苗菌的稳定性。重组载体pIRES-hpaA通过脂质体法转染CCXS-7细胞,SDS-PAGE及Western印迹法检测pIRES-hpaA表达HpaA蛋白的免疫原性。结果 成功扩增出长约750bp的hpaA基因,测序结果表明扩增出的hpaA基因与H．pylorihpaA序列一致,PCR和酶切鉴定结果证实hpaA基因克隆入真核表达载体pIRES,成功构建了含hpaA基因的幽门螺杆菌核酸疫苗pIRES-hpaA,并成功构建了幽门螺杆菌hpaA基因的减毒鼠伤寒沙门菌核酸疫苗,重组核酸疫苗稳定,Western印迹法检测到了特异性的蛋白条带。结论 构建了具有免疫反应性的H．pylori hpaA减毒鼠伤寒沙门菌核酸疫苗,为进一步探索其免疫作用奠定了基础。     

幽门螺杆菌感染及其根除治疗对血清胃泌素水平的影响

幽门螺杆菌感染及其根除治疗对血清胃泌素水平的影响６３００３８重庆第三军医大学西南医院陈洁平徐采朴关键词幽门螺杆菌；治疗；胃泌素中国图书资料分类号Ｒ５７３幽门螺杆菌（Ｈｐ）与慢性胃炎（ＣＧ）及消化性溃疡（ＰＵ）发生的关系已经得到普遍认可。近年来的研究表...     

硫糠铝加抗生素治疗幽门螺杆菌相关的十二指肠溃疡疗效观察

硫糠铝加抗生素治疗幽门螺杆菌相关的十二指肠溃疡疗效观察武警福建总队医院内科陈传邦，李焕金，蔡辉，陈宇翔（福州３５００１９）关键词硫糖铝，抗生素，幽门螺杆菌，十二指肠溃疡我院自１９９５年１月～１９９６年１２月，应用硫糖铝加四环素、灭滴灵三联治疗幽门螺杆...     

Knee injury and Osteoarthritis Outcome Score (KOOS) - validation of a Swedish version

The Knee injury and Osteoarthritis Outcome Score (KOOS) is a self-administered instrument measuring outcome after knee injury at impairment, disability, and handicap level in five subscales. Reliability, validity, and responsiveness of a Swedish version was assessed in 142 patients who underwent arthroscopy because of injury to the menisci, anterior cruciate ligament, or cartilage of the knee. The clinimetric properties were found to be good and comparable to the American version of the KOOS. Comparison to the Short Form-36 and the Lysholm knee scoring scale revealed expected correlations and construct validity. Item by item, symptoms and functional limitations were compared between diagnostic groups. High responsiveness was found three months after arthroscopic partial meniscectomy for all subscales but Activities of Daily Living.     

Endovascular management of carotid-cavernous fistulas

Objective To investigate endovascular treatment of traumatic direct carotid-cavernous fistulas （CCF） and their complications such as pseudoaneurysms. Methods： Over a five-year period, 22 patients with traumatic direct CCFs were treated endovascularly in our institution. Thirteen patients were treated once with the result of CCF occluded, 8 twice and 1 three times. Treatment modalities included balloon occlusion of the CCF, sacrifice of the ipsilateral internal carotid artery with detachable balloon, coll embolization of the cavernous sinus and secondary pseudoaneurysms, and covered-stem management of the pseudoaneurysms. Results All the direct CCFs were successfully managed endovascularly. Four patients developed a pseudoaneurysm after the occlusion of the CCF with an incidence of pseudoaneurysm formation of 18.2% （4/22）. A total number of 8 patients experienced permanent occlusion of the ICA with a rate of ICA occlusion reaching 36.4% （8/22）. Followed up through telephone consultation from 6 months to 5 years, all did well with no recurrence of CCF symptoms and signs. Conclusion Traumatic direct CCFs can be successfully managed with endovascular means. The pseudoaneurysms secondary to the occlusion of the CCFs can be occluded with stent-assisted coiling and implantation of covered stents.     

The acutely limping child

Acute limping may be the result of multiple pathologies in children. The differential diagnosis varies based on the age of the child. Irrespective of age, the initial imaging work-up includes AP and frog leg radiographs of the pelvis and ultrasound; MRI may sometimes be helpful. In children less than 3 years, infections and trauma are most frequent. MRI is the imaging modality of choice when osteomyelitis is clinically suspected. Between the ages of 3 and 10 years, transient synovitis of the hip and Legg-Calvé-Perthes disease are main considerations but infection, inflammation and focal bony lesions are also considered. In children over 10 years, slipped capital femoral epiphysis also is considered.     

Do Balance Board Training Programs Reduce the Risk of Ankle Sprains in Athletes?

Introduction Ankle sprains are the most common musculo-skeletal injury that occurs in athletes,particularly in sports that require jumping and landing on one foot such as soccer,and basketball(1-4).These injuries often result in significant time loss from participation,long-term disability,and have a major impact on health care costs and resources(5-8).     

High Intensity Interval Training:New Insights

KEY POINTS ·High-intensity interval training(HIT)is characterized by repeated sessions of relatively brief，intermittent exercise.often performed with an“a11 out”effort or at an intensity close to that which elicits peak oxygen uptake(i.e.，≥90%of VO2 peak).     

Developmental validation of the PowerPlex(®) ESI 16 and PowerPlex(®) ESI 17 Systems: STR multiplexes for the new European standard

In response to the ENFSI and EDNAP groups’ call for new STR multiplexes for Europe, Promega^® developed a suite of four new DNA profiling kits. This paper describes the developmental validation study performed on the PowerPlex^® ESI 16 (European Standard Investigator 16) and the PowerPlex^® ESI 17 Systems. The PowerPlex^® ESI 16 System combines the 11 loci compatible with the UK National DNA Database^®, contained within the AmpFlSTR^® SGM Plus^® PCR Amplification Kit, with five additional loci: D2S441, D10S1248, D22S1045, D1S1656 and D12S391. The multiplex was designed to reduce the amplicon size of the loci found in the AmpFlSTR^® SGM Plus^® kit. This design facilitates increased robustness and amplification success for the loci used in the national DNA databases created in many countries, when analyzing degraded DNA samples. The PowerPlex^® ESI 17 System amplifies the same loci as the PowerPlex^® ESI 16 System, but with the addition of a primer pair for the SE33 locus. Tests were designed to address the developmental validation guidelines issued by the Scientific Working Group on DNA Analysis Methods (SWGDAM), and those of the DNA Advisory Board (DAB). Samples processed include DNA mixtures, PCR reactions spiked with inhibitors, a sensitivity series, and 306 United Kingdom donor samples to determine concordance with data generated with the AmpFlSTR^® SGM Plus^® kit. Allele frequencies from 242 white Caucasian samples collected in the United Kingdom are also presented. The PowerPlex^® ESI 16 and ESI 17 Systems are robust and sensitive tools, suitable for the analysis of forensic DNA samples. Full profiles were routinely observed with 62.5 pg of a fully heterozygous single source DNA template. This high level of sensitivity was found to impact on mixture analyses, where 54–86% of unique minor contributor alleles were routinely observed in a 1:19 mixture ratio. Improved sensitivity combined with the robustness afforded by smaller amplicons has substantially improved the quantity of data obtained from degraded samples, and the improved chemistry confers exceptional tolerance to high levels of laboratory prepared inhibitors.