Modulation of tumour necrosis factor-α-induced cytotoxicity by polyphenols

The effects of several aromatic compounds on tumour necrosis factor-α (TNF)-induced cytotoxicity in murine fibroblast L929 cells were studied. Those phenolics which bear the O -dihydroxy aromatic structure (catechols), i.e. caffeic acid, catechol, 3,4-dihydroxybenzoic acid, dopamine and noradrenaline effectively inhibited TNF-induced cytotoxicity in a concentration dependent manner. Related compounds (cinnamic acid, 3,4-dimethoxycinnamic acid, 2,4-dihydroxybenzoic acid, ferulic acid, 3-hydroxy-4-methoxybenzoic acid and p -hydroxybenzoic acid) which lack the catecholic functional group failed to protect cells from TNF cytotoxicity. All concentrations of catechols which effectively inhibited the TNF-induced cytotoxicity were also protective as a post treatment, suggesting an effect at some step(s) in the TNF-signal transduction pathway following receptor activation. © 1997 John Wiley & Sons, Ltd.     

薄层扫描法测定穿心莲中穿心莲内酯、脱水穿心莲内酯的含量

采用薄层扫描法测定了 15批穿心莲商品药材中穿心莲内酯、脱水穿心莲内酯的含量 ,并进行了比较。结果脱水穿心莲内酯高于穿心莲内酯的占 2 6 .7% ,穿心莲内酯高于脱水穿心莲内酯的占 5 3.3% ,两者含量差异在±10 %以内的占 2 0 .0 %。     

Pharmacokinetic and Pharmacodynamic Interaction of Andrographolide and Standardized Extract of Andrographis paniculata (Nees) with Nabumetone in Wistar Rats

The aim of the study was to investigate the herb–drug interaction of Andrographis paniculata Nees (Acanthaceae) and Andrographolide (AN) with nabumetone (NAB) in wistar rats. Pharmacokinetic and pharmacodynamic interactions were studied after co‐administration of APE and AN with NAB in Wistar rats. In pharmacokinetic studies, significant decrease in Cmax, AUC_0–t and AUC_0–∞ of 6‐MNA after co‐administration with pure AN and APE has been observed. T_max of 6‐MNA has been increased to 2 h from 1.5 h in AN + NAB treated group. Changes in mean residential time, clearance and volume of distribution of 6‐MNA in APE + NAB treated group and AN + NAB treated group indicated interference of other components of APE other than AN. In pharmacodynamic study, significant decrease in antiarthritic activity of NAB on concomitant administration with APE and AN has been observed. The study concludes that NAB exhibits pharmacokinetic and pharmacodynamic interactions with APE and AN in rats thus alarms the concomitant use of herbal preparations containing APE and AN with NAB. Further study is needed to understand the mechanism and predict the herb–drug interaction in humans. Copyright © 2016 John Wiley & Sons, Ltd.     

穿心莲R2R3-MYB基因家族的鉴定与表达分析

R2R3-MYB是一类调控植物生长发育及次生代谢的重要转录因子.利用生物信息学手段从穿心莲全基因组中鉴定R2R3-MYB基因,分析其理化性质、染色体定位信息、基因结构和保守序列信息、系统进化信息及启动子区域的顺式作用元件.结合转录组测序技术和qRT-PCR分析其在非生物胁迫和激素诱导下的表达谱.结果 表明,穿心莲R2R...     

黄芩甙对重症急性胰腺炎大鼠胰腺ICAM-1和P-选择素表达的影响

目的观察黄芩甙对重症急性胰腺炎(SAP)大鼠胰腺组织ICAM-1和P-选择素表达的影响情况。方法将45只大鼠随机分为假手术组、模型组、黄芩甙组,每组15只。假手术组进腹后仅翻动胰腺和十二指肠后关腹,模型组、黄芩甙组在造模成功后分别给予生理盐水和黄芩甙。术后3,6,12 h,观察大鼠胰腺的病理变化,测定胰腺组织中髓过氧化物(MPO)和肿瘤坏死因子-α(TNF-α)的含量,用免疫组化方法检测胰腺组织ICAM-1和P-选择素的表达情况。结果术后3,6,12 h,模型组和黄芩甙组的病理评分均显著高于假手术组(P均<0.05),且模型组和黄芩甙组6,12 h的病理评分均显著高于3 h(P均<0.05),但2组评分在6 h和12 h间无显著性差异;术后3,6,12 h,模型组胰腺组织中MPO和TNF-α较假手术组显著降低(P均<0.05),黄芩甙组胰腺组织中MPO和TNF-α较模型组显著降低(P均<0.05);术后各时间点,模型组ICAM-1和P-选择素随时间延长显著增多,黄芩甙组则随时间延长而显著减少。结论黄芩甙可能通过抑制中性粒细胞的浸润,减轻ICAM-1和P-选择素的表达而发挥对胰腺组织的保护作用。     

何首乌总苷抗氧化与实验性小鼠主动脉粥样硬化病变的形成

目的：研究何首乌总苷(polygonum multiflorum total glycoside,PMTG)的抗氧化性及对载脂蛋白E基因缺陷(apoprotein E gene deficiency,ApoE-/-)小鼠实验性动脉粥样硬化(atherosclerosis,As)病变形成的影响。方法：将ApoE-/-小鼠随机分为4组：何首乌总苷高剂量组(150 mg·kg^-1·d^-1)、低剂量组(25 mg·kg^-1·d^-1)、阿托伐他汀阳性药物组(5 mg·kg^-1·d^-1)及模型组,给药第10周后全部处死,酶法检测4组血清脂质含量；化学法测氧化指标一氧化氮(NO)、总抗氧化能力(TAC)及丙二醛(MDA)；电镜观察主动脉壁内皮细胞形态变化以及免疫组织化学染色方法测定ApoE-/-小鼠主动脉壁NFκB的表达。结果：实验10周后,何首乌总苷高、低剂量组及阳性药物组与模型组比较,显示：①可以显著降低血清总胆固醇(TC),甘油三酯(TG)及低密度脂蛋白-胆固醇(LDL-C)水平(P<0.01)；②明显增加血浆NO及TAC(P<0.05及P<0.01),减少MDA的生成(P<0.01)；③电镜观察发现何首乌总苷可以维持主动脉壁内皮细胞正常形态；④抑制ApoE-/-小鼠主动脉壁核因子-κB（NF-κB）的表达(P<0.01)。结论：何首乌总苷可能通过抗氧化保护主动脉内皮细胞形态,降低ApoE-/-小鼠氧化型LDL(ox-LDL)、减少主动脉壁NF-κB的表达,从而下调主动脉壁ICAM-1及VCAM-1的表达等环节延缓主动脉斑块的形成,起到防止ApoE-/-小鼠实验性动脉粥样硬化病变形成的作用。     

CML-1 inhibits TNF-alpha-induced NF-kappaB activation and adhesion molecule expression in endothelial cells through inhibition of IkBalpha kinase

CML-1 is a purified extract from a mixture of 13 oriental herbs (Achyranthis Radix, Angelicae Gigantis Radix, Cinnamomi Cortex Spissus, Eucommiae Cortex, Glycyrrhizae Radix, Hoelen, Lycii Fructus, Paeoniae Radix, Rehmanniae Radix Preparata and Atractylodis Rhizoma, Zingiberis Rhizoma, Zizyphi Semen, Acori Graminei Rhizoma) that have been widely used for the treatment of inflammatory diseases in Asia. Since our previous study has been shown to have the anti-inflammatory activity of CML-1 in vivo and the upregulation of adhesion molecules in response to numerous inducing factors is associated with inflammation, this study examined the effect of CML-1 on the expression of adhesion molecules induced by TNF-alpha in cultured human umbilical vein endothelial cells (HUVECs). Preincubation of HUVECs for 20h with CML-1 (1-100mug/ml) dose-dependently inhibited TNF-alpha (10ng/ml)-induced adhesion of THP-1 monocytic cells, as well as mRNA and protein expression of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). CML-1 was also shown to inhibit NK-kB activation induced by TNF-alpha. Furthermore, CML-1 inhibited TNF-alpha-induced IkB kinase activation, subsequent degradation of IkBalpha, and nuclear translocation of NK-kB. Evidence presented in this report demonstrated that CML-1 inhibited the adhesive capacity of HUVEC and the TNF-alpha-mediated induction of E-selectin, ICAM-1 and VCAM-1 in HUVEC by inhibiting the IkB/NF-kB signaling pathway at the level of IkB kinase, which may explain the ability of CML-1 to suppress inflammation and modulate the immune response.     

简易穴位埋线对哮喘大鼠ICAM-1和NF-kB表达及气道炎症的影响

目的:探讨简易穴位埋线对哮喘大鼠的作用机制.方法:Wistar大鼠32只,随机分为简易穴位埋线组(A)、地塞米松组(B)、模型组(C)、对照组(D),每组8只.卵蛋白(OVA)致敏制造大鼠哮喘模型,A组:在实验第1天开始造模前埋线1次,穴取"肺俞""膻中""肾俞".B组:自实验第15天开始腹腔注射地塞米松1.5 mg/kg;C组:于实验第15天起腹腔注射灭菌注射用水20 mg/kg;B、C组均1次/日,连续2周.D组:不做任何处理.观察4组大鼠哮喘发作症状,对各组大鼠支气管肺泡灌洗液进行细胞计数及分类,并对大鼠气道病理组织学改变进行观察;免疫组化方法观察细胞间黏附分子-1(ICAM-1)和核因子-kB(NF-kB)的核着色情况.结果:①简易穴位埋线组大鼠哮喘发作症状减轻,支气管周围炎细胞浸润明显减少.肺泡灌洗液总细胞数及其他炎细胞数模型组明显高于简易穴位埋线组.简易穴位埋线组气道腔内炎性细胞渗出和气道壁炎性细胞浸润明显低于模型组.②模型组肺组织ICAM-1、NF-kB的表达显著高于对照组(P<0.01);③地塞米松组、简易穴位埋线组肺组织ICAM-1、NF-kB的表达与模型组相比显著降低(P<0.05,P<0.01).结论:简易穴位埋线可抑制ICAM-1、NF-kB表达,减轻气道炎症.     

复方丹参饮对胰岛素抵抗大鼠血管内皮细胞ICAM-1、VCAM-1表达的研究

目的:探讨复方丹参饮对胰岛素抵抗(IR)大鼠血清中细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)的表达。方法:将60只Wistar雄性大鼠适应性的喂养1周,随机分为生理盐水组、胰岛素抵抗组、复方丹参饮预防组(中药预防组)复方丹参饮治疗组(中药治疗组)、罗格列酮组(西药对照组),每组分为12只。采用高脂高糖高胆固醇饮食喂养,建立大鼠IR模型。喂养16周以后采用酶联免疫法检测各组Wistar大鼠血清中ICAM-1、VCAM-1的含量。结果:与生理盐水组比较,除复方丹参饮预防组差异无统计学意义外(P>0.05),其余各组大鼠血清中ICAM-1、VCAM-1的含量均升高(P<0.05),有统计学意义;与胰岛素抵抗组相比较,西药对照组、中药治疗组与中药预防组均能减少大鼠血清中ICAM-1、VCAM-1的含量(P<0.05)。结论:认为复方丹参饮具有抑制大鼠血清血管内皮细胞ICAM-1、VCAM-1的过度表达作用,对胰岛素抵抗大鼠的血管内皮细胞具有保护功效。     

加味三棱丸对子宫内膜异位症黏附分子ICAM-1，CD44表达的影响

目的:研究加味三棱丸(SLW)对子宫内膜异位症移植物的抑制作用和探讨对黏附分子ICAM-1,CD44表达的影响.方法:采用子宫内膜自体移植法建立大鼠子宫内膜异位症模型,用游标卡尺、HE染色和免疫组化分别检测子宫内膜异位症移植物体积变化、异位内膜组织形态学改变和黏附分子ICAM-1,CD44的表达.结果:SLW 0.11,1.02 g·kg~(-1)均可以明显减小子宫内膜异位症移植物的体积;显微形态显示:给药组与模型组比较其病理改变程度减轻,异位内膜为薄单层细胞排列而非腺体数目多,细胞呈多层排列,腺上皮呈扁平或矮立方形,未见假腺体改变;SLW 1.02,0.11 g·kg~(-1)均能明显降低了异位子宫内膜黏附分子ICAM-1,CD44的蛋白表达.结论:SLW可显著抑制子宫内膜异位症移植物生长,其作用机制可能与抑制黏附分子ICAM-1,CD44的蛋白表达水平有关.     

痰热清注射液对急性脑梗死患者血中IL-1β及ICAM-1水平的影响

目的观察痰热清注射液治疗急性脑梗死的临床疗效及其作用机制。方法将急性脑梗死患者65例随机分为两组，治疗组35例予以痰热清注射液，对照组30例给予清开灵注射液，疗程14d；检测治疗前后血清IL-1β及ICAM-1水平，同时进行神经功能评分比较。结果治疗组临床疗效优于对照组；两组治疗后血清IL-1β及ICAM-1水平较治疗前显著降低，而治疗组降低更明显。结论痰热清注射液治疗急性脑梗死安全有效，其作用机制可能为降低了IL-1β及ICAM-1水平。     

In Vitro and In Vivo Aldose Reductase Inhibitory Activity of Standardized Extracts and the Major Constituent of Andrographis paniculata

Aldose reductase is the first enzyme in the polyol pathway and catalyzes the reduction of glucose to sorbitol by coupling with the oxidation of NADPH to NADP⁺. This sorbitol accumulation leads to various diabetic complications, including neuropathy, nephropathy, cataracts, and retinopathy. In the present study, aldose reductase inhibitory (ARI) activity of the methanolic as well as standardized extracts of Andrographis paniculata (Burm. f.) Wall. ex Nees (Acanthaceae) and its chief constituent, andrographolide, were studied using in vitro and in vivo methods. In the in vitro method, rat lens as well as kidney homogenates were used for the preparation of enzyme, whereas the effect of these test samples on the galactitol level in the eye lens was studied in a galactosemic rat model in vivo. The results of the study revealed that both extracts of the plant and its major compound, andrographolide, possess ARI activity in vitro. They were also found to significantly decrease galactitol accumulation in vivo. Copyright © 2012 John Wiley & Sons, Ltd.     

电项针疗法对脑缺血再灌注大鼠ICAM-1及ICAM-1 mRNA表达的影响

目的:观察电项针疗法对脑缺血再灌注后大鼠脑缺血半暗区细胞间粘附分子-1表达的影响。方法:运用栓线法制作局灶性脑缺血再灌注大鼠模型,随机将大鼠分成4组:假手术组、模型组、电体针组和电项针组,随机又将各组大鼠分为12 h、1 d、3 d和5 d共4个时点。通过HE染色的方法观察病理形态学的变化;应用免疫组织化学方法和原位杂交技术检测脑缺血半暗区ICAM-1和ICAM-1 mRNA的表达。结果:HE染色显示:与模型组相比,治疗组缺血半暗区的神经细胞肿胀程度减轻,胶质细胞增生,新生毛细血管和神经元的数量增多;电项针组明显于电体针组。治疗组可在早期抑制ICAM-1蛋白及其mRNA的表达(P<0.05,P<0.01)。同时间点电项针组与电体针组比较有显著性差异(P<0.05)。结论:电项针疗法可有效减轻神经元的损伤程度;电项针疗法可以下调再灌注后缺血区细胞间粘附分子-1蛋白及其mRNA的表达,从而达到防治脑缺血再灌注炎性损伤的目的。     

脂可平对实验性早期动脉粥样硬化氧自由基及主动脉ICAM-1表达的影响

目的:研究脂可平对大鼠早期动脉粥样硬化氧自由基及细胞间黏附分子(ICAM-1)表达的影响。方法:应用高脂饲料复制SD大鼠早期动脉粥样硬化模型,分别给以脂可平、辛伐他汀片混悬液灌胃,实验10周后,全自动生化分析仪检测各组大鼠血脂、血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,用免疫组化和RT-PCR方法检测主动脉壁ICAM-1及其mRNA的表达水平;苏木素伊红(HE)染色观察主动脉组织形态学变化。结果:两治疗组血脂、血清MDA含量均明显降低,SOD活性显著升高,不同程度的改善了主动脉组织病理损伤,ICAM-1及其mRNA的表达水平显著下降。结论:脂可平抗动脉粥样硬化作用的机制可能与其抗氧化、抗粘附等保护血管内皮细胞功能密切相关。     

引种不同来源穿心莲叶中穿心莲内酯及脱水穿心莲内酯积累分析

目的观察不同来源穿心莲叶中穿心莲内酯和脱水穿心莲内酯的积累,以期为品种选育提供依据。方法收集同一引种地各生长阶段的不同来源地穿心莲叶,以HPLC测定叶中穿心莲内酯和脱水穿心莲内酯的含量。结果经数据分析,穿心莲内酯和脱水穿心莲内酯与生长期呈现明显的相关性。穿心莲内酯含量无论大叶型还是小叶型与始花期均成正相关,即开花期越晚,生长期越长,含量越高,大叶型相关系数的平均值为0.951,小叶型为0.899;脱水穿心莲内酯含量与始花期均成负相关,即开花期越晚,脱水穿心莲内酯含量越低,大叶型相关系数均值为-0.930,小叶型为-0.979。结论建议穿心莲品种应从大叶型中选育始花期晚的品种。     

复方丹参饮对胰岛素抵抗大鼠血清ICAM-1、VCAM-1表达的影响

目的:探讨复方丹参饮对胰岛素抵抗大鼠血清ICAM-1、VCAM-1表达的影响。方法:取健康成年Wistar雄性大鼠60只,随机分为正常对照组、模型对照组、中药预防组、中药治疗组、罗格列酮组,每组12只。采用高脂高糖高胆固醇饮食建立大鼠IR模型;各组大鼠灌胃给药16周后,采用酶免法检测各组大鼠血清中ICAM-1、VCAM-1的含量。结果:与正常对照组比较,除中药预防组,其余各组ICAM-1、VCAM-1含量均增加(P0.05);与模型对照组比较,罗格列酮组及中药预防组、治疗组均能减少ICAM-1、VCAM-1的含量(P0.05)。结论:复方丹参饮可以抑制血清ICAM-1、VCAM-1的表达,防治血管内皮损伤。     

牛磺酸通过p38通路对缺氧内皮细胞ICAM-1,VCAM-1表达的影响

探讨牛磺酸(taurine,Tau)通过p-p38通路对牛肺主动脉内皮细胞(PAECs)中ICAM-1,VCAM-1的影响及其作用机制。原代培养PAECs取4~12代细胞用于实验。分为5组:对照(control)组、缺氧(hyp)组、抑制剂(SB203580)组、给药(Tau)组、抑制剂+给药(SB+Tau)组,Tau的给药浓度为100 mmol·L~(-1),p38抑制剂SB203580浓度为20μmol·L~(-1),给药时间为12h。MTT检测不同浓度Tau对PAECs的抑制。使用Western blot及Real-time PCR方法检测p38通路蛋白及炎症因子ICAM-1,VCAM-1的表达情况。使用免疫荧光检测p38核位移情况。MTT结果显示随着Tau浓度增加,对PAECs增殖抑制增强。Western blot和Real-time PCR结果显示在蛋白水平及基因水平Tau都抑制ICAM-1,VCAM-1的表达。Western blot的结果和免疫荧光的结果均显示Tau可以抑制p38蛋白的活化。Tau可能通过p38 MAPK通路抑制由缺氧引起的牛肺主动脉内皮细胞中ICAM-1,VCAM-1的表达。     

泄浊化瘀降脂灵对高血脂症大鼠肝脏ICAM-1、MCP-1表达的影响

目的通过观察泄浊化瘀降脂灵对高血脂症大鼠的血脂以及肝脏黏附分子-1(ICAM-1)、单核细胞趋化因子-1(MCP-1)表达的影响,探讨泄浊化瘀降脂灵对高脂血症大鼠肝脏的保护机制。方法将大鼠随机分成空白组、模型组、辛伐他汀组以及浊化瘀降脂灵高、低剂量组,采用高脂饲料复制高血脂症大鼠模型。应用酶法检测血中TC、TG、HDL-C、LDL-C的含量,光镜观察肝脏形态学的改变,免疫组织化学法观察肝组织细胞间ICAM-1、MCP-1的蛋白表达。结果①与空白组比较,模型组造模后TC、TG、LDL-C水平显著升高(P<0.05或0.01),HDL-C水平显著降低(P<0.01),提示各组大鼠造模成功。②与〗模型组比较,泄浊化瘀降脂灵高剂量组、辛伐他汀组TC、TG及LDL-C水平显著降低(P均<0.01),HDL-C水平显著升高(P<0.01)。泄浊化瘀降脂灵高、低剂量组比较亦有显著性差异(P均<0.05)。③与模型组比较泄浊化瘀降脂灵高剂量组及辛伐他汀组肝脏ICAM-1、MCP-1的OD值均显著降低(P均<0.01)。泄浊化瘀降脂灵高剂量组与辛伐他汀组比较无显著性差异(P均>0.05)。结论泄浊化瘀降脂灵高剂量可下调MCP-1及ICAM-1高表达,减少、减轻肝细胞脂肪变性和炎症反应,保护高血脂症大鼠的肝脏。     

当归对单侧输尿管梗阻大鼠肾组织中黏附分子ICAM-1表达的影响

目的:观察当归对单侧输尿管梗阻(UUO)大鼠肾小管间质纤维化过程中黏附分子P-选择素(P-selectin)和细胞间黏附分子-1(ICAM-1)、转化生长因子-β1(TGF-β1)等细胞因子的表达和CD68单核/巨噬细胞浸润的影响。方法:采用大鼠模型,52只SD大鼠分为:假手术组、UUO组、当归组。术后第7d和第14d留取标本处死大鼠,用免疫组化方法检测肾组织内P-selectin、ICAM-1、CD68、TGF-β1的表达。结果:与假手术组相比,UUO组大鼠第7d和第14d肾组织内ICAM-1、P-selectin、CD68、TGF-β1的表达均显著上调(P<0.05)。与UUO组相比,当归组两个时间段肾小管间质病变减轻,且肾组织内ICAM-1、CD68、TGF-β1的表达受到显著抑制(P<0.05)。结论:当归可能是通过抑制ICAM-1的表达和炎细胞的组织浸润,从而降低了肾小管间质TGF-β1的水平和纤维化的进展。     

含碘中药对甲亢大鼠细胞间黏附分子表达的影响

目的:观察不同浓度含碘中药对甲亢大鼠模型甲状腺组织细胞黏附分子ICAM-1、VCAM-1 mRNA表达的影响。方法:制作大鼠甲状腺机能亢进模型,灌服不同浓度含碘中药复方及其他对照药物进行治疗,然后RT-PCR法检测各组大鼠甲状腺组织ICAM-1、VCAM-1 mRNA水平。结果:含碘较低的中药复方较之含碘高的中药复方在减少ICAM-1及VCAM-1 mRNA表达上具有显著性的差异。结论:小剂量含碘中药复方对实验性甲亢大鼠具有抑制其甲状腺细胞黏附分子表达,保护甲状腺细胞的作用。