白鹤藤化学成分研究

目的 研究白鹤藤(Argyreia acuta Lour.)的化学成分.方法 采用硅胶柱色谱、Sephadex LH-20柱色谱、ODS柱色谱、MCI柱色谱、半自动制备液相色谱等手段进行分离纯化,通过理化性质和波谱解析鉴定化合物的结构.结果 从白鹤藤的乙酸乙酯部位和石油醚部位分离得到8个化合物,分别鉴定为3,4-0-二咖啡酰基奎宁酸甲酯(1)、3,4-0-二咖啡酰基奎宁酸乙酯(2)、3,5-0-二咖啡酰基奎宁酸丁酯(3)、3,4-O-二咖啡酰基奎宁酸丁酯(4)、木栓醇(5)、木栓酮(6)、菠甾醇(7)、豆甾醇(8).结论 化合物1～7首次从该属植物中分离得到.     

艾纳香石油醚部位化学成分的研究

目的:对黔产艾纳香石油醚部位的化学成分进行研究.方法:采用硅胶、凝胶等柱色谱技术对艾纳香石油醚部位的化学成分进行分离、纯化,再结合核磁共振技术鉴定其化学结构.结果:分离并鉴定了5个化合物,分别为花椒油素(1)、豆甾醇(2)、三十一烷(3)、龙脑(4)、4,22-二烯-3-酮豆甾烷(5).结论:5个化合物均首次从艾纳香石油醚部位分离得到.     

艳丽齿唇兰的化学成分研究(I)

目的研究艳丽齿唇兰石油醚提取部位的化学成分。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱及制备薄层方法对艳丽齿唇兰石油醚部位进行分离、纯化,通过理化常数和波谱分析鉴定化合物的结构。结果从艳丽齿唇兰的石油醚萃取部位分离鉴定出6个化合物,包括2个木栓烷型三萜、1个羽扇豆烷型三萜、2个植物甾醇和1个长链脂肪烷烃,其结构分别鉴定为:木栓酮(1)、木栓烷(2)、豆甾醇(3)、sorghumol(4)、十八烷(5)、菜油甾醇(6)。结论 6个化合物均为首次从该植物中分离得到,化合物2为首次从开唇兰属植物中分离得到。     

白花败酱石油醚部位化学成分研究

目的 对白花败酱石油醚部位化学成分进行研究。方法 采用硅胶柱色谱、Sephadex-20柱色谱及高效液相色谱等进行分离纯化,运用波谱学方法进行结构鉴定。结果 从白花败酱石油醚分离得到8个化合物,分别鉴定为熊果酸（化合物1）,豆甾醇（化合物2）,松脂素（化合物3）,ent-eudesm-4（15）-ene-1β,6α-diol（化合物4）,波甾醇（化合物5）,阿魏酸（化合物6）,5,3′,4′-三羟基-3,7-二甲氧基黄酮（化合物7）,乌苏酸内酯（化合物8）。结论 化合物3、化合物4、化合物5、化合物7、化合物8为首次从白花败酱中分离得到。     

桑椹的化学成分研究

目的 研究桑椹的化学成分.方法 采用硅胶柱色谱、ODS柱色谱、Sephadex LH-20柱色谱、制备HPLC等色谱方法进行分离纯化,采用理化性质结合波谱数据解析鉴定化合物的结构.结果 从桑椹的70%(体积分数)乙醇提取物中分离得到7个化合物,分别鉴定为aurantiamide(1),细胞松弛素B(2),(22E)-24-methyl-5α-cholesta-7,22-diene-3β,5α,6β-triol(3),(22E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol(4),7β-羟基谷甾醇(5),β-谷甾醇(6),胡萝卜苷(7).结论 化合物1~5为首次从桑椹中分离得到.     

两色紫金牛化学成分研究

目的 研究两色紫金牛的活性化学成分.方法 应用柱色谱分离化学成分,波谱方法鉴定化合物结构.结果 从两色紫金牛脂溶性部位得到5个化合物,分别鉴定为岩白菜素(1),11-O-(3‘,5‘-二羟基-4‘-甲氧基没食子酰基)-岩白菜素(2),11-O-(3‘,4‘,5‘-三羟基没食子酰基)-岩白菜素(3),β-谷甾醇(4)和豆甾醇(5).结论 化合物1～5均为首次从该种植物中分离得到,其中化合物2为首次从该属植物中分离得到.     

中国南海海绵Jaspis stellifera中异臭椿型萜类成分研究

目的:对采自中国南海海域海绵Jaspis stellifera中异臭椿型萜类成分进行分离鉴定.方法:采用硅胶柱色谱、凝胶柱色谱和HPLC制备色谱和重结晶方法分离纯化得到单体化合物,采用有机波谱方法鉴定化合物结构.结果:从Jaspis stellifera氯仿层萃取物中分离得到5个化合物,分别鉴定为22,23-dihydrostellettin B(1)、isogeoditin A(2)、stellettin A(3)、stellettin B (4)、jaspolide B(5).结论:化合物1和2为首次从该属海绵中分离得到的异臭椿型萜类成分.     

白花蛇舌草化学成分研究

目的 研究白花蛇舌草的化学成分.方法 用硅胶柱色谱对其化学成分进行分离,应用波谱学方法鉴定结构.结果 从该植物中分离出6个化合物,分别鉴定为:乙酰羽扇豆醇酯(1)、咖啡酸(2)、3,4-二羟基苯甲酸(3)、5-羟基-6,7,3′,4′-四甲氧基黄酮(4)、槲皮素(5)、山奈酚(6). 结论 化合物1～4为首次从该植物中分离得到.     

宁夏枸杞叶中的化学成分

目的研究中药宁夏枸杞叶中的化学成分。方法使用70%乙醇对宁夏枸杞叶进行提取,所得浸膏混悬后依次用石油醚、乙酸乙酯、正丁醇进行萃取。分别取乙酸乙酯层和正丁醇层,使用硅胶柱色谱、凝胶柱色谱、制备液相色谱及薄层色谱等色谱技术进行分离纯化,利用理化常数和核磁共振、质谱等波谱学技术对分离得到的化合物进行结构鉴定。结果从宁夏枸杞叶70%乙醇提取物乙酸乙酯萃取层和正丁醇萃取层中分离鉴定了10个化合物,分别为对甲氧基苯甲酸(1),对甲氧基肉桂酸(2),东莨菪素(3),对羟基苯甲醛(4),对羟基苯乙酮(5),(Z)-2-亚乙基-3-甲基丁二酸(6),槲皮素(7),腺苷(8),东莨菪苷(9),芦丁(10)。结论化合物1,2,4,6,8为首次从枸杞属植物中分离得到,化合物3,5,9为首次从宁夏枸杞叶中分离得到。     

蔓荆子黄酮类化学成分的研究

目的 研究蔓荆子中的黄酮类化学成分.方法 采用Sephadex LH-20色谱柱、聚酰胺柱色谱等手段进行分离纯化,通过理化性质和波谱解析对结构进行鉴定.结果 从蔓荆子75%(体积分数)乙醇提取液中分离得到6个化合物:蒿黄素(1)、4'-羟基-5,6,7-三甲氧基黄酮(2)、蔓荆子黄素(3)、5,4'-二羟基-6,7,-二甲氧基黄酮(4)、山奈酚(5)、槲皮素(6).结论 化合物2和化合物4为首次从该植物中分离得到.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Electro-Acupuncture Combined with the Trigger Point Needle-Embedding for Treatment of Primary Trigeminal Neuralgia in 31 Cases

In recent years, the author of this essay has applied electro-acupuncture combined with the trigger point needle-embedding for treatment of primary trigeminal neuralgia in 31 cases, yielding satis- factory results as reported in the following.     

Investigation of Matrix Metalloproteinase -1, 2 and tissue inhibitor of matrix metalloproteinases-1 in Endometriosis

Objective： To explore the role of matrix metalloproteinase-1,2 （MMP-1, MMP-2） and tissue inhibitor of matrix metalloproteinases-1 （TIMP-1） in endometriosis. Methods： The eutopic and ectopic endometria from 40 subjects suffering from endometriosis and regular.endometria from 40 subjects （excluding endometriosis） were collected and examined by in situ hybridization technology and western blot assay. Results： Both expressions of MMP-1 and -2 were stronger in ectopic endometrium and eutopic endometrium than in normal endometrium. On the contrary, the expression of TIMP-1 in ectopic endometrium and eutopic endometrium was lower. The differences were significant （P 〈 0.01 ）. Moreover, there was no relationship among the expressions of MMP-1, 2 and TIMP-1 in ectopic endometrium. Conclusion： The expressions of MMP-1, 2 and TIMP-1 lose balance and lack of periodic changes in ectopic endometrium , which explains the biological invasive behavior of endometriosis. It was suggested-that regulating the balance between the MMPs and TIMP-1 should be an ideal therapeutic target to endometriosis.     

Shi Da-zhuo (史大卓) —An outstanding cardiologist of integrative medicine

Prof. SHI Da-zhuo, Ph.D., male, was born on March 20, 1960. Prof. SHI entered the Ph.D. program in 1990 at the China Academy of Chinese Medical Sciences under the supervision of Prof. CHEN Ke-ji, majoring in the treatment of cardiovascular diseases. After receiving his Ph.D. degree in 1993, Prof. SHI started working at the Cardiovascular Center in Xiyuan Hospital affiliated to China Academy of Chinese Medical sciences.     

Persist in and Carry Forward the Integrative Medical Research——Speech at the 6th National General Congress of Chinese Association of Integrative Medicine by Academician CHEN Zhu,Minister of Ministry of Health

The 6th National General Congress of Chinese Association of Integrative Medicine （CALM） was convened at 19-20, April 2008 in Beijing. Academician CHEN Zhu, the minister of Ministry of Health indicated at the congress that the integration of Chinese and Western medicine is very well in keeping with the situation of our country and the general rule of development in medical science; and as a good integration of Chinese medicine and Western medicine, it is mutually beneficial and advantageous to both of them. Seeing the creativity shown in integrative medical investigation in theoretic and methodological sides, we should and must persist in and develop it.