大豆胚芽中新异黄酮化合物的研究

目的 分离鉴定大豆胚芽中异黄酮的组成。方法 利用柱色谱技术从大豆胚芽中分离异黄酮，利用MS，^1H—NMR，^13C—NMR鉴定得到的异黄酮化合物的结构。结果 分离到两个新异黄酮化合物，这2个异黄酮化合物互为同分异构体，相对分子质量为564，经鉴定为6″-β-D-阿拉伯糖-染料木素葡萄糖苷(I)，6″-β-D-木糖-染料木素葡萄糖苷(Ⅱ)。结论 这两个化合物在大豆中为首次分离到，也是两个新化合物。     

新疆鼠尾草根水溶性成分的研究

目的：探讨不同种鼠尾草根中的水溶性成分，通过对新疆鼠尾草根的研究，以寻找水溶性有效成分。方法：用水提取、分离精制得到新化合物，并与丹参中的水溶性成分进行比较。结果：利用^1H-NMR、^13C-NMR、^1H-^1H和^1C-^13C COSY及HMBC谱对新化合物结构中所有氢和碳信号进行了归属。结论：新疆鼠尾草中的新化合物为丹酚酸K，它同样具有咖啡酸衍生物的结构类型。     

N-取代-5-磺酰基吡唑肟酯衍生物的合成及生物活性研究

目的为了获得更好生物活性的吡唑类衍生物。方法以吡唑酮、盐酸羟胺、硫酚等化合物为原料合成吡唑新化合物，通过光谱鉴定其结构，检测其生物活性。结果设计合成了5个未见文献报道的吡唑肟醚化合物。其结构经元素分析、^1H NMR、IR确认。结论初步生物活性测试表明，一些化合物具有一定的生物活性。     

盾叶薯蓣水溶性成分的研究

目的研究盾叶薯蓣的水溶性化学成分，寻找活性物质。方法用系统溶剂提取，色谱法分离，光谱法鉴定结构，如：^1H—NMR、^13C—NMR135DEPT、HMQC、HMBC、TOCSY。结果从盾叶薯蓣的水溶液中分离到-个薯蓣皂苷、一个苯苷类化合物、一个生物碱类化合物。薯蓣皂苷经测定分析，鉴定为26-O-（β-D-葡萄糖）-（25R）-furost-5-en-3β，26-diol-22-OMe-3-O-{α-L-鼠李糖-（1→4）-[β-D-葡萄糖-（1→3）-β-D-葡萄糖-（1→2）]-β-D-葡萄糖}。结论经文献检索为一新化合物，命名为盾叶薯蓣皂苷E（zingierenin E），另两个化合物在盾叶薯蓣中为首次报道。     

马蹄莲中有机酸的分离与结构鉴定

目的分离并鉴定马蹄莲中的有机酸。方法将乙醇提取物用硅胶柱色谱分离，分离得到有机酸，其化学结构经UV、IR、^1H—NMR、^13C—NMR及GC—MS波谱分析确证。结果从马蹄莲中分离得到两种化合物：棕榈酸、肉豆蔻酸。结论两种化合物均系首次从马蹄莲中分离得到。     

大豆茎中两个新鞘脂类化合物

目的：解析两个新化合物的平面结构。方法：应用硅胶柱层析等分离技术分离化合物，根据理化性质及IR，MS，NMR等光谱数据鉴定结构。结果：鉴定两个化合物结构为N-2-(1，3，4-三羟基-十八烷基)-α-羟基-二十五烷酰胺(Ⅰ)，N-2-(1，3，4-三羟基-十八烷基)-α-羟基-二十四烷酰胺(Ⅱ)。结论：化合物Ⅰ，Ⅱ均为新化合物，分别命名为大豆鞘脂A和大豆鞘脂B。     

墨旱莲活性成分对刀豆蛋白A诱导的小鼠肝损伤的作用

目的研究中药墨旱莲活性成分对刀豆蛋白A（ConA）诱导的小鼠肝损伤的作用。方法以尾静脉注射ConA（15mg／kg）建立小鼠肝损伤模型,检测墨旱莲提取物对正常小鼠和肝损伤小鼠血清谷丙转氨酶（ALT）的影响,观察肝脏的组织学改变。生物活性跟踪分离得到单体化合物,根据IR谱、^13C—NMR谱、^1H—NMR谱、^1H-^13CHMBC谱和1H-^13CHMQC谱鉴定其结构。结景墨旱莲提取物能降低肝损伤小鼠的血清ALT水平,对抗ConA诱导的小鼠肝细胞凋亡。体外筛选的活性化合物经波谱鉴定为蟛蜞菊内酯,属香豆素类化合物,能选择性抑制ConA诱导的T细胞活化,10μg／mL的蟛蜞菊内酯可完全拮抗ConA的诱导作用。结论中药墨旱莲具有抗ConA诱导的小鼠肝损伤作用,活性成分蟛蜞菊内酯具有较强的抗炎和抗凋亡作用,是一个潜在的抗炎保肝新药的先导化合物。     

黄花乌头中生物碱类化学成分的研究

目的进一步研究毛茛科植物黄花乌头Aconitum coreanum的化学成分，寻找更多有活性的生物碱类物质。方法采用乙醇提取、溶剂萃取、氧化铝柱色谱方法进行分离纯化有关成分，根据理化性质和谱学分析(IR，ESI—MS，^1H—NMR，^13C—NMR，^1H-^1HCOSY，HMQC，HMBC)进行结构鉴定。结果从黄花乌头根中分离得到7个化合物，分别鉴定为：11，13-二乙酰基-14-羟基-2-异丁酰基-赫替新(11，13-diacetyl-14-hydroxy-2-isobutyryl hetisine，Ⅰ)、关附庚素(guan—fubase G，Ⅱ)、关附己素(guan—fu base F，Ⅲ)、关附Z素(guan—fu base Z，Ⅳ)、关附壬素(guan—fu base I，Ⅴ)、关附子素(guan—fu baseK，Ⅵ)、关附胺醇(guan—fu aminealcohol，Ⅶ)。结论化合物Ⅰ为新天然产物，命名为关附巳素(guan—fu base P)，首次报道其氢谱和碳谱数据。     

银杏叶中一个新的萜内酯类成分

目的： 研究银杏（Ginkgo biloba L.）叶中新的化学成分。方法： 运用柱色谱方法分离纯化,利用UV、IR、MS、_{1H NMR、¹³}C NMR等色谱技术鉴定化合物结构。结果： 从银杏叶中分离鉴定出1个新化合物：1,7,10-三羟基-3,14-去氢银杏内酯(Ⅱ)和1个已知化合物：银杏内酯L(ginkgolide L)(Ⅰ)。结论： 化合物Ⅱ为新化合物,命名为银杏内酯N（ginkgolide N）;化合物Ⅰ的光谱数据为首次报道。     

甘遂中巨大戟萜醇型二萜酯类化学成分的研究

目的 分离鉴定甘遂Euphorbia kansui中巨大戟萜醇(ingenol)型二萜酯类化学成分。方法 运用波谱技术，尤其是2DNMR技术(^1H-^1HCOSY,HSQC,HMBC和NOESY实验)进行结构鉴定。结果 分离得到6个巨大戟萜醇型二萜酯，确定了其中两个主要化合物的结构，分别为巨大戟萜醇—3—2’E，4‘Z癸二烯酯—20—乙酸酯[3—O—(2’E，4‘Z—decadienoyl)—20—O—acetylingenol](Ⅰ)和甘遂大戟萜酯C(kansuiphorin C)(Ⅱ)。结论 首次报道了I的全部NMR数据和Ⅱ的碳谱数据，并利用多种2DNMR技术对Ⅰ和Ⅱ的全部NMR数据进行了归属。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.