熊去氧胆酸治疗妊娠期肝内胆汁淤积症疗效探讨

目的评价熊去氧胆酸（UDCA）治疗妊娠期肝内胆汁淤症（ICP）的效果。方法86例患者,随机分成两组。治疗组46例,采用UDCA250mg,每日3次口服;对照组40例,予能量合剂500ml静滴,每日1次,两组均以10天为1个疗程,观察疗效。结果使用UDCA治疗ICP,不仅改善患者临床症状和血生化异常,还可延长ICP患者胎儿孕龄,减少胎儿窒息和新生儿窘迫的发生。结论UDCA是治疗ICP安全、有效的药物。     

还原型谷胱甘肽辅助熊去氧胆酸对妊娠期胆汁郁积症患者肝功能及母婴结局的影响

目的:研究还原型谷胱甘肽辅助熊去氧胆酸对妊娠期胆汁郁积症(Intrahepatic cholestasis of pregnancy,ICP)患者肝功能及母婴结局的影响.方法:选取柘城县人民医院66例ICP患者(2019年9月至2020年12月),根据治疗方案不同分为对照组(33例)和观察组(33例),均给予常规治疗,对照组采用熊去氧胆酸治疗,观察组采用还原型谷胱甘肽辅助熊去氧胆酸治疗.于治疗4周后比较两组临床疗效、肝功能指标、免疫功能指标,持续跟踪随访直至胎儿娩出观察两组母婴结局.结果:治疗后观察组总有效率高于对照组(P<0.05);且肝功能指标、免疫功能指标值改善较好(P<0.05);观察组早产、胎儿宫内窘迫、新生儿窒息发生率均低于对照组.结论:还原型谷胱甘肽辅助熊去氧胆酸治疗ICP患者临床疗效确切,可有效改善肝功能、免疫功能.     

妊娠期肝内胆汁淤积症与新生儿黄疸的关系

目的探讨妊娠期肝内胆汁淤积症(ICP)与新生儿黄疸的关系。方法选择36例ICP患者为观察组,36例同期来院分娩的正常孕妇为对照组,分别测定孕妇血清总胆汁酸水平及新生儿血清总胆红素水平。结果ICP孕妇血清总胆汁酸的水平及新生儿血清胆红素水平均较对照组明显增高,观察组新生儿血清胆红素水平明显增高且72h内黄疸发生率增高。结论母体发生ICP可使新生儿黄疸发生率增高。     

熊去氧胆酸联合低分子肝素对妊娠期肝内胆汁淤积症患者TNF-α、VEGF的影响

目的探讨熊去氧胆酸及低分子肝素对妊娠期肝内胆汁淤积(ICP)症患者治疗中的价值及相关各项指标的变化。方法选取2009年6月～2010年12月在我院住院的ICP患者。分为2组,对照组为在常规西药治疗方法,观察组在常规西药治疗方法同时加用低分子肝素4000 IU皮下注射+熊去氧胆酸。比较2组治疗前后孕妇肝功能、空腹胆汁酸水平及血清TNF-α、VEGF变化。比较2组产后胎盘TNF-α变化。结果观察组及对照组治疗前后肝功能及空腹胆汁酸、VEGF均下降,观察组较对照组下降明显,差异有显著性。2组治疗前后血清TNF-α升高,差异无显著性。产后观察组胎盘TNF-α较对照组明显降低,差异有显著性。结论熊去氧胆酸+低分子肝素对ICP患者可有效改善治疗效果。联合治疗为临床治疗提供了新思路。     

腺苷蛋氨酸治疗妊娠肝内胆汁淤积症疗效探讨

目的探讨腺苷蛋氨酸(S-adeocy-L-methionime,SAMe)治疗妊娠肝内胆汁淤积(intrahepatic cholestasis of pregnancy,ICP )的疗效.方法 2000年10月至2001年 12月在我院产科住院的ICP孕妇118例,随机分为2组SAMe观察组 50例和能量对照组68例,观察组给予SAMe注射剂500mg加入10%GS500ml中静滴,每日1次;对照组给予能量合剂500ml静滴,每日1次;7d 1疗程.观察肝功能转氨酶(ALT及AST)及血清胆汁酸(TBA)水平,比较分娩孕周、新生儿体重、羊水情况、新生儿窒息及围产儿死亡.结果 SAMe治疗组肝功能及血清胆汁酸水平较对照组明显下降,胎儿窘迫、新生儿窒息及围产儿死亡发生较对照组明显减少,孕周延长,新生儿体重较重.结论 SAMe可以改善ICP患者的妊娠预后,故认为SAMe治疗ICP有效.     

中西医结合治疗妊娠期肝内胆汁淤积症80例分析

目的 评估中西结合治疗妊娠期肝内胆汁淤积症(IcP)的效果。方法 选择80例IcP病人，随机分成两组，观察组(n＝40)采用中药胆淤冲剂、能量合剂、地塞米松、熊去氧胆酸(UDCA)治疗。对照组(n=40)采用熊去氧胆酸(UDCA)口服观察治疗效果。结果 观察组治疗2w后血ALT、TBA明显下降(P＜0.05)，并对控制度瘙痒效果明显，而对照组下降缓慢。并义观察组围产儿病死率、围产儿病率明显低于同期报道的发生率。且胎儿窘迫、羊水污染、早产均较对照组明显降低，差异极显著(P＜0．05)。结论 中西医结合疗法对母儿无毒副作用，是治疗ICP安全有效的方法。     

血清甘胆酸筛查妊娠期肝内胆汁淤积症的优势探讨

为探讨空腹血清甘胆酸(CG)检测对于筛查妊娠期胆汁淤积症(ICP)患者的优势,本文对400名正常孕妇和87例胆淤症妊娠患者的空腹血清CG、总胆汁酸(TBA)、总胆红素(TBIL)及丙氨酸氨基转移酶(ALT)检测结果进行回顾性分析.结果显示,ICP组血清CG值非常显著高于正常组(P＜0.001),其阳性率为100%,并早于TBA、TBIL和ALT,而后三者仅轻中度升高,阳性率分别为44.8%、11.5%和35.6%.因此,血清CG筛查为孕妇普查及时发现ICP的灵敏的经济的早期的血清学指标.     

120例ICP患者血清中CG、TBA与TSH水平变化分析

目的探讨妊娠期肝内胆汁淤积症（ICP）患者血清中甘胆酸（CG）、总胆汁酸（TBA）与促甲状腺激素（TSH）水平的变化及其临床意义。方法选取妊娠期肝内胆汁淤积症（ICP）患者120例为实验组,对照组为94例正常妊娠者,分别测定其甘胆酸（CG）、总胆汁酸（TBA）与促甲状腺激素（TSH）水平并进行比较。结果与正常妊娠组相比,ICP患者血清中CG、TBA及TSH水平均显著升高,差异有统计学意义（P〈0.05～0.01）。结论监测ICP患者血清中CG、TBA及TSH水平的变化对判断病情,指导临床具有重要价值。     

妊娠期肝内胆汁淤积症102例母儿围生结局分析

目的探讨妊娠期肝内胆汁淤积症（ICP）对围产儿预后的影响。方法收集2007年1月至2007年12月在我院分娩的ICP孕妇102例为ICP组,设观察同期分娩的102例正常孕妇为对照组,比较两组间胎儿窘迫、羊水粪染、新生儿窒息、早产的发生率。结果 ICP组胎儿窘迫、羊水粪染、新生儿窒息、早产分别占：53.92%、37.25%、6.86%、14.71%;与对照组比较,差异有显著性。结论妊娠期肝内胆汁淤积症易突发胎儿宫内缺氧,对已确诊的妊娠期肝内胆汁淤积症患者加强治疗及胎儿监护、适时终止妊娠,可降低围生儿死亡率、患病率。     

甘利欣联合熊去氧胆酸对慢性乙型肝炎GGT的疗效观察

目的探讨甘利欣联合熊去氧胆酸(UDCA)治疗GGT持续升高的慢性乙型肝炎患者的疗效。方法治疗组31例给予甘利欣联合UDCA治疗,对照组30例只用甘利欣治疗,疗程2周。观察治疗组与对照组血清GGT值的变化。结果慢性乙型肝炎轻度患者中,治疗组与对照组治疗后GGT值相比无显著性差异(P>0.05),慢性乙型肝炎中度患者中,治疗组与对照组治疗后GGT值相比有明显差异(P<0.05)。结论应用甘利欣和UDCA治疗慢性乙型肝炎能有效降低GGT,且无明显副作用。     

An amino acid transporter involved in gastric acid secretion

Gastric acid secretion is regulated by a variety of stimuli, in particular histamine and acetyl choline. In addition, dietary factors such as the acute intake of a protein-rich diet and the subsequent increase in serum amino acids can stimulate gastric acid secretion only through partially characterized pathways. Recently, we described in mouse stomach parietal cells the expression of the system L heteromeric amino acid transporter comprised of the LAT2-4F2hc dimer. Here we address the potential role of the system L amino acid transporter in gastric acid secretion by parietal cells in freshly isolated rat gastric glands. RT-PCR, western blotting and immunohistochemistry confirmed the expression of 4F2-LAT2 amino acid transporters in rat parietal cells. In addition, mRNA was detected for the B⁰AT1, ASCT2, and ATB(0+) amino acid transporters. Intracellular pH measurements in parietal cells showed histamine-induced and omeprazole-sensitive H⁺-extrusion which was enhanced by about 50% in the presence of glutamine or cysteine (1 mM), two substrates of system L amino acid transporters. BCH, a non-metabolizable substrate and a competitive inhibitor of system L amino acid transport, abolished the stimulation of acid secretion by glutamine or cysteine suggesting that this stimulation required the uptake of amino acids by system L. In the absence of histamine glutamine also stimulated H⁺-extrusion, whereas glutamate did not. Also, phenylalanine was effective in stimulating H⁺/K⁺-ATPase activity. Glutamine did not increase intracellular Ca²⁺ levels indicating that it did not act via the recently described amino acid modulated Ca²⁺-sensing receptor. These data suggest a novel role for heterodimeric amino acid transporters and may elucidate a pathway by which protein-rich diets stimulate gastric acid secretion.P. Kirchhoff and M.H. Dave contributed equally to this study and therefore share first authorship     

Improved method for simultaneous determination of ascorbic acid and dehydroascorbic acid, isoascorbic acid and dehydroisoascorbic acid in food and biological samples.

The total vitamin C amount in different food and plasma samples was determined by a dual detection system, after HPLC separation, with direct detection of ascorbic acid and indirect fluorimetric detection of dehydroascorbic acid after a post-column O-phenyldiamine derivatisation. The two active forms of vitamin C and their D-isomers were separated within 10 min. The repeatability was determined by measurement of several fruits and vegetables and ranged from 0.3 to 1.9% (relative standard deviation) for vitamin C. The reproducibility, based on double determinations, ranged from 1.9 to 3.6% for vitamin C, depending on the matrix. The reproducibility, based on several determinations of reference materials, ranged from 2.4 to 3.7% for ascorbic acid and from 4.3 to 5.8% for dehydroascorbic acid, again depending on the matrix.     

Antagonism of sulfadoxine and pyrimethamine antimalarial activity in vitro by p-aminobenzoic acid, p-aminobenzoylglutamic acid and folic acid

The activity of pyrimethamine and sulfadoxine against two strains of Plasmodium falciparum has been studied in vitro by a radioisotopic technique. Low level antagonism of pyrimethamine resulted from the inclusion of p-aminobenzoic acid, p-aminobenzoylglutamic acid or folic acid in the test medium. Sulfadoxine activity was antagonised slightly by p-aminobenzoic but not by p-aminobenzoylglutamic acid, and antagonised markedly by folic acid at concentrations above 4 X 10(-8) M. At 10(-7) M folic acid, a concentration lower than that of normal RPMI medium 1640, sulfadoxine activity was reduced 7000 to 9000-fold in comparison with controls. These results are of importance in terms of the utilisation of folates by P. falciparum, the susceptibility of the parasite to antifolate drugs and the in vitro determination of parasite susceptibility.     

Heats of mixing of polyriboadenylic acid and polyribouridylic acid in aqueous NaCl solution

The heats of mixing of polyriboadenylic acid (Poly A) and polyribouridylic acid (Poly U) in various proportions by changing the mole ratio n = [Poly U]/[Poly A] in 0,001, 0,1, and 0,5 molar NaCl solutions containing 0,1 molar tris-HCl buffer solution were measured. Constant values for the heats of mixing of the (Poly A+n Poly U) system are found for n ≤ 1 and n ≥ 2. From the results of UV and CD spectra, it may be considered that the mixing of Poly A and n (Poly U) leads to the formation of Poly(A+U) for n ≤ 1, and to that of Poly(A+2U) for n ≥ 2, even at [NaCl] = 0,001 mol/l. The enthalpies of formation of Poly(A+U) and that of Poly(A+2U) depend on the concentration of NaCl. This dependence may be attributed to the differences in conformation of Poly(A+U) in NaCl solution.