抗精子抗体在女性不孕患者中的临床意义

目的探讨女性不孕症患者血清抗精子抗体的临床意义。方法采用ELISA方法检测AsAb。结果不孕症患者的血清抗精子抗体阳性率显著高于正常生育妇女。结论部分原因不明的女性不孕症可能与抗精子抗体有关。     

不孕夫妇血清中抗精子抗体的检测

作者应用浅盘凝集试验（ＴＡＴ）检测不明原因不孕夫妇血清抗精子抗体，结果抗体阳性率为３０．０４％，其中不孕女方阳性３７．５％，不育男方阳性２８．５７％，本文就抗精子抗体的发生与不孕症的关系，诊断及临床意义进行了讨论。     

不孕夫妇血清中抗精子抗体的检测

作者应用浅盘凝集试验（ＴＡＴ）检测不明原因不孕夫妇血清抗精子抗体。结果抗体阳性率为３０．０４％，其中不孕女方阳性３７．５％，不育男方阳性２８．５７％。本文就抗精子抗体的发生与不孕症的关系、诊断及临床意义进行了讨论。     

910例不孕症患者抗精子抗体测定的结果分析

目的了解不孕症与抗精子抗体的相关性。方法用ELISA法检测不孕症患者血清中的抗精子抗体。结果不孕症患者血清中的抗精子抗体的阳性检出率明显高于正常对照组。讨论抗精子抗体与不孕症有明显的相关性。     

原因不明不孕患者抗精子抗体的测定及其临床意义

目的：测定原因不明不孕患者血清中抗精子抗体,探讨抗精子抗体在免疫性不孕症中的作用。方法：采用试管－－玻片凝集试验检测原因不明不孕患者血清中抗精子抗体,计数１００－２００个活动精子,包括游离精子数（ＮＦ）和凝集精子数（ＮＡ）,以ＮＡ／ＮＡ＋ＮＦ≥１０％为阳性,并与体格检查健康的育龄非孕状态妇女对照。结果：试管－－玻片凝集试验方法简单,结果直观,准确度高,无假阳性结果,在原因不明不孕患者中,抗精子抗体     

超声粉碎精子作抗原包被测定抗精子抗体

采用高能超声粉碎精子作包被抗原,使用酶联免疫吸附测定技术(ELISA),检测52对不育夫妇、10对有生育能力夫妇血清中的抗精子抗体(Antisperm Antilodies,ASA)。不育组中阳性率为37.5％(39/104),对照组阳性率为5％(1/20),有显著性差异。本实验改进了传统的精子抗原包被方法,实验费用低,操作简单,实为一种高效实用的检测血清抗精子抗体的新方法。     

免疫斑点法检测血抗精子抗体及其临床应用

据文献报道 ,患不育症夫妇占育龄夫妇的 8%～ 15 % 〔1,2〕。不育的原因十分复杂 ,在免疫因素中尤以抗精子抗体(ＡｓＡｂ)与某些不育症的发生最为密切 ,占原因不明的不育症患者的 2 0 %左右〔1,2〕。作者应用免疫斑点法检测血抗精子抗体 ,并与ＥＬＩＳＡ法进行比较。现报道如下。1　材料与方法1.1　研究对象　对照组为 30对正常生育夫妇 ,孕妇妊娠 2 5周以上 ;不孕症夫妇 5 3对 ;习惯性流产夫妇 2 6对。1.2　标本采集　抽取男女双方静脉血各 1.5ｍｌ,分离血清 ,经 5 6℃ 30ｍｉｎ灭能 ,备用。1.3　阳性标准血清、阴性标准血清、ＥＬＩＳＡ试…     

精子结合抗体在不孕症中的检测应用

目的：研究精子结合抗体（MAR）与不明原因的不孕症的相关性。方法：对不同孕症失妇110对采用直接法检测男性精液中的精子结合抗体。间接法检测女性血清和生殖安泌物中的精子结合抗体。结果：共检出阳性人数43例,其中女性阳性率为24．5％,男性阳性率为14．5％。结论：精子结合本的存在可以影响受精的各个环节,引起不孕。     

抗精子抗体和不明原因的不育症

本文应用浅盘凝集试验和精子宫颈粘液接触试验分别检测34对不明原因不育夫妇血清和生殖道局部分泌物中抗精子抗体的发生率。15/34对一方血清抗精子抗体阳性(44.12％),女方阳性率为23.53％,男方阳性率为20.59％。12/27对一方局部抗精子抗体阳性(44.44％),宫颈粘液抗体阳性率为25.92％,精液抗体阳性率为18.52％。5例仅有血清抗体,6例仅有局部抗体,6例兼有血清和局部抗体。结果显示不明原因不育症患者中血清和局部抗精子抗体发生率明显高于生育组,而且血清和局部抗体活性可能不一致。因此在研究免疫不育症时同时检测夫妇双方血清和局部抗精子抗体有重要意义。     

抗精子抗体与男性精液参数的临床分析

目的探讨抗精子抗体(AsAb)导致男性不育的机制。方法对107例不孕夫妇的丈夫精液中有凝集现象和297例不明原因的不孕夫妇的丈夫进行抗精子抗体检测,利用蛋白芯片法检测其血清中的AsAb-IgM。将AsAb-IgM( )的精液与正常生育男性的精液各参数相比较。结果107例不孕夫妇的丈夫精液中有凝集现象的AsAb-IgM( )为57%(61/107),297例不明原因的不孕夫妇的丈夫AsAb-IgM( )为5.7%(17/297)。这78例抗精子抗体阳性的精液在精液量,密度、pH值等方面无显著性差异(P>0.05)。但是在精子活力(特别是a级精子的百分率)、活率、宫颈粘液(CM)穿透力方面有显著性差异(P<0.01)。结论抗精子抗体的存在使精液的总体质量有显著的降低,是男性不育或生育力降低的一个重要的原因。     

无精子症118例的配偶血清抗精子抗体

检查2208对不育夫妇的精液和抗精抗体,丈夫无精子118人中,其妻抗精抗体阳性47例,占39.8%;有精子2090人中,其妻抗精抗体阳性470例,占22.5%,无精子者配偶的抗精抗体检出率显著升高。无精子者的精桨成分可能是诱发抗精抗体的免疫原;其配偶抗精抗体发生率高可能归因于男性抑制物质下降。提示对无精子不育夫妇作供精人工授精时,应排除女方的抗精抗体。     

单次上游单次离心高活力精子分离与宫腔内授精

介绍了一种用于宫腔内授精（ＩＵＩ）的高活力精子分离方法一单次上游单次离心法，活性精子提取率为（１９．９±６．３）％，其中，ａ精子提取率为（３２．０±１３．８）％。提取的精子悬液中，ａ精子率为（８３．８±５．５）％。精子活动率约９５％，畸形精子率下降了６个百分点。应用上述方法进行精子分离对２７对男性少、弱、畸精子症致不育夫妇进行了４０个周期的ＩＵＩ治疗，妊娠率（ＰＧＲ）２０％。ＩＵＩ中精子数量超过６×１０＿６，ＰＧＲ为３２％，而不足６×１０＿６者无一例妊娠（Ｐ＜０．０５）。     

不孕夫妇精子－宫颈粘液体外穿透试验与其他不孕因素的关系研究

应用玻片法精子－宫颈粘液穿透试验检测３５０例不孕夫妇。结果显示，不孕夫妇穿透试验评分差和阴性者占６０％。３６例为各项检测正常，仅穿透试验评分差，占１０％。穿透试验评分优良的比率与精子活率、向前直线运动精子的比率以及女性血清抗精子抗体阳性的比率有关。     

Male sterilisation: its aspects and effects

Nonappliance and appliance methods of contraception, together with abortion, serve as means of spacing rather than limiting the number of children couples have. Only sterilization limits population. There are various problems with sterilization that have to be dealt with in order to make it a more effective and trusted method. Some of these problems are: 1) infection following the operation; 2) faulty or incomplete instructions given as to limitations and possibility of existing sperm after the operation; 3) poststerilization pregnancies; 4) decreases in sexual satisfaction following sterilization; and 5) fears of impotency.     

Effectiveness of in vitro fertilization with intracytoplasmic sperm injection for severe male infertility

BACKGROUND: In its 1993 report the Canadian Royal Commission on New Reproductive Technologies challenged the effectiveness of in vitro fertilization for severe male infertility. To address the Commission's concern, the authors compared the relative effectiveness of in vitro fertilization combined with intracytoplasmic sperm injection for severe male infertility and conventional in vitro fertilization for complete tubal occlusion in women. METHODS: This historical cohort study was done at the PROCREA Fertility Centre, a private tertiary human reproduction centre in Montreal. Three groups of infertile couples were compared: 122 couples with severe male infertility treated by in vitro fertilization with intracytoplasmic injection of fresh sperm from ejaculate (group 1); 27 couples with obstructive azoospermia treated by in vitro fertilization with intracytoplasmic injection of epididymal sperm (collected by microepididymal or percutaneous epididymal sperm aspiration) (group 2); and 98 couples with tubal factor infertility (bilateral tubal occlusion) treated with conventional in vitro fertilization (with sperm from ejaculate) (group 3). The main outcomes measured were rates of fertilization, pregnancy, clinical pregnancy and implantation. RESULTS: Pregnancy rates per started cycle were 35%, 40% and 34% for groups 1, 2 and 3 respectively. When prognostic factors were controlled for, none of the outcome measures differed significantly between the 3 groups. INTERPRETATION: In vitro fertilization with intracytoplasmic injection of sperm from the ejaculate or the epididymis is as effective for treating severe male infertility as conventional in vitro fertilization is for treating complete occlusion of the fallopian tubes in women.     

ELISA法检测Zeta链联合血红蛋白电泳对地中海贫血筛查的价值

目的探索适用于农村地中海贫血（thalassemia,地贫）的筛查模式。方法对南宁市郊农村750对育龄夫妇行血常规检测,平均红细胞体积（mean cell volume,MCV）下降者定义为地贫表型阳性。夫妇一方或双方地贫表型阳性者联合酶联免疫吸附剂测定（enzyme-linked immunosorbent assay,ELISA）法检测Zeta链和国产电泳仪行血红蛋白电泳,与地贫基因结果进行比对。结果 750对育龄夫妇中,261对（34.8%）夫妇任一方地贫表型阳性,接受Zeta链和血红蛋白电泳检测。97对夫妇一方HbA2增高者行β地贫基因检测,检出4对（0.41%）β地贫高危夫妇,即夫妻双方均携带β地贫基因,电泳结果与基因检测结果一致。α地贫基因检测结果表明,119例（22.7%）受试者携带--SEA。Zeta链诊断--SEA的漏诊率为5.9%（7例,其中5例为HbH病患者）,误诊率为0.5%（2例）。以夫妇双方任一方Zeta阳性为标准,11对α地贫高危夫妇均可筛查出。结论ELISA法检测Zeta链联合血红蛋白电泳法是一种有效、经济、方便、易于在农村地区应用的地贫筛查模式。     

抗精子抗体ELISA检测法及其应用

建立了用精子可溶性抗原包被的ELISA法,检测了100例处女血清,均为阴性,17例有生育能力者和1000例不育患者血清的阳性率分别为5.9％和22％,抗体检出率符合国外多数学者的结果。重复性试验定性符合率达97％。表明用该法检测抗精子抗体具有敏感、特异、稳定等特点,可获较为满意的结果。     

Interpretation of semen analysis among infertile couples.

Among the male partners of 1074 infertile couples the mean results of semen analysis were sperm count 78 X 10(6)/ml, seminal volume 4.0 ml, proportion of progressively motile sperm 54%, proportion of sperm with normal morphologic features 81.4% and total motile sperm count 152.3 X 10(6) per ejaculate. After excluding 65 couples who chose donor insemination and 300 with known female causes of infertility, the cumulative pregnancy rates in the remaining 709 couples were higher with increasing sperm density and motility and seminal volume, but the higher rates were significant only when these variables were combined into total motile sperm count per ejaculate. The cumulative pregnancy rates were 20% with a total motile sperm count of 9 X 10(6) or less, 37% with a count of 10 to 19 X 10(6) and 52% with a count of 20 X 10(6) or more (p = 0.001). Counts higher than 20 X 10(6) were not associated with a further improvement in pregnancy rates, but variability in the results was high, which suggests that the test should be repeated as necessary to determine the true range. Although standards for these and other seminal variables are ill defined, the total motile sperm count incorporates the most useful prognostic information from semen analysis, and the associated pregnancy rates can help guide clinical decisions.