乳腺癌石蜡切片中显示雌,孕激素受体方法和阳性结果判断标…

为了探索雌，孕激素受体的检测方法和阳性结果判断标准的规范化，应用ＥＲ，ＰＲ单克隆抗体免疫组化Ｓ－Ｐ法检测乳腺癌石蜡切片中ＥＲ，ＰＲ的表达。结果显示：２００例ＥＲ及ＰＲ的阳性率分别为７３．５％及６５．５％。用单克隆抗体可直接显示ＥＲ，ＰＲ，结果可靠，敏感性高，重复性好，且与目前国际通用方法接轨。     

乳腺Paget病细胞角蛋白-8、c-erbB-2、雌激素受体和孕激素受体的表达及意义

目的探讨乳腺Paget病中细胞角蛋白-8(CK-8)、c-erbB-2、雌激素受体(ER)和孕激素受体(PR)的表达及意义。方法对12例乳腺paget病组织进行免疫组织化学染色,检测CK-8、c-erbB-2、ER、PR在Paget细胞与同一例深部癌组织中表达的异同。结果Paget细胞CK-8表达阳性率为100%、c-erbB-2表达阳性率为91.67%,而ER、PR表达阳性率为16.67%。CK-8、c-erbB-2、ER及PR中每一标记物在Paget细胞和同一病例深部癌组织中阳性表达一致。结论乳腺Paget病内Paget细胞来源于乳腺深部存在的导管内癌或浸润性导管癌,是深部癌细胞在表皮内的扩散。     

用改良LSAB法检测乳腺癌雌激素受体和孕激素受体

用改良ＬＳＡＢ法检测乳腺癌雌激素受体和孕激素受体唐建林，高东宸乳腺癌雌激素受体（ＥＲ）和孕激素受体（ＰＲ）检测已经成为估计乳腺癌预后和内分泌治疗效果的主要方法。我们的实验以结合于ＥＲ，ＰＲ上的内源性激素为检测目标，以抗激素抗体为第一抗体，采用ＬｓＡＢ...     

表皮生长因子受体与雌,孕激素受体在乳腺癌组织中的表达及相互关系

本文应用碘标记的表皮生长因子，用分步离心提取部细胞膜蛋白，以标准的ＥＧＦ作竞争剂，采取放射标记结合法测定了４０例乳腺癌组织中的表皮生长因子受体水平，同时采用葡聚糖活性碳分析法测定了乳腺癌组织中的雌激素受体，孕激素受体以探讨ＥＧＦＲ表达与ＥＲ，ＰＲ在乳腺癌组织中的生物学行为及相互关系。     

人乳腺癌雌激素受体的免疫组织化学测定

一、材料和方法人乳腺癌标本由上海市房地产管理局职工医院、瑞金医院和市第九医院收集，贮于一SOC；6－7［3H］－E2购自中科院原子核研究所；DAB和兔一PAP－IgG均为Sigma公司产品；兔抗小鼠IgG和羊抗兔IgG均为上海生物制品研究所产品；抗人乳腺癌雌激素受体（ER）单抗（IH12）为本所研制[1]。PAP免疫级化法：将冻存的标本作厚SPin冰冻切片，室温放置10分钟，3．7％福马林固定15分钟，浸入甲醇和丙酮各3分钟，10％小牛血清封闭15分钟，分别滴加ER单抗（1H12腹水1：10）和无关腹水（阴性对照）、PBS（空白对照），湿盒内1小时…     

103例乳腺癌组织学与雌激素受体的研究

应用生物素抗生物素过氧化酶法对103例乳腺癌石蜡切片进行雌激素受体检测,结果发现雌激素受体阳性率在导管内癌中较高,占71%,而在浸润性导管癌、髓样癌中较低,分别占57%、29%。分析了雌激素受体和组织类型关系,探讨了它和组织分级及核分级之间的联系。雌激素受体阳性率随组织分级升高而降低,随核的分级升高而增加。说明乳腺癌组织分化好,雌激素受体表达倾向阳性,而分化低的肿瘤,雌激素受体表达倾向阴性。雌激素受体阳性患者比阴性患者复发率低,存活时间长,预后好,其检测有助于判断患者预后,安排合理的治疗方案,提高治愈率。     

子宫腺肌症雌激素受体、孕激素受体与bcl-2的表达

目的 探讨雌激素受体（ER）、孕激素受体（PR）和bcl-2在子宫腺肌症中异位和在位内膜的表达及意义。方法 用免疫组化EnVision法检测40例子宫腺肌症在位子宫内膜和肌间异位内膜ER、PR和bcl-2的表达情况。结果 在位和异位子宫内膜组织中均有ER、PR和bcl-2的阳性表达。其中腺上皮阳性表达率高于间质（P〈0．05）,且异位内膜的腺上皮bcl-2阳性表达率高于在位组织（P〈0．05）;ER和PR的表达两者差异无显著性（P〉0．05）。异位内膜腺体ER、PR与bcl-2表达具有相关性（P〈0．01）。结论 子宫腺肌症异位和在位子宫内膜组织中均有ER、PR和bcl-2的阳性表达,可能与子宫腺肌症的发生、发展有关。     

胰腺癌雌、孕激素受体水平与临床病理的关系

近年来,胰腺癌发病率逐步上升,由于胰腺癌的化疗和放疗效果不甚理想,所以性激素受体和胰腺癌的关系越来越引起人们的重视,使内分泌治疗可望成为胰腺癌治疗的新方法。本文对５６例胰腺癌组织进行雌激素受体（ＥＲ）、孕激素受体（ＰＲ）检测,旨在探讨胰腺癌与临床病理...     

ER、PR及其相应拮抗剂与甲状腺癌的相关性研究进展

雌激素受体(ER)、孕激素受体(PR)在甲状腺癌中的异常表达说明性激素受体在甲状腺癌的发病机制中起重要作用.ER、PR通过经典的基因途径及非基因途径对甲状腺癌的发生、发展及生物学特征产生重要影响.性激素受体拮抗剂抑制甲状腺癌细胞增殖作用的研究,将为甲状腺癌的治疗提供新的选择方案.     

Expression of androgen receptors in benign and malignant endometrial stromal neoplasms

Several studies have shown that endometrial stromal neoplasms express estrogen and progesterone receptors (ER, PR). To our knowledge, the presence or absence of androgen receptors (AR) in these rare uterine neoplasms has not been investigated. Tumors (n=20)—3 endometrial stromal nodules, 14 low-grade endometrial stromal sarcomas (ESS, low grade), and 3 high-grade endometrial sarcomas (undifferentiated endometrial sarcoma, UES)—were studied. Immunohistochemical analyses for ER, PR, and AR were performed on formalin-fixed, paraffin-embedded archival material. Positive immunoreactions for ER and PR were observed in 14 (70%) and 17 (85%) cases, respectively. Furthermore, 9 cases (45%) were positive for AR. Among 17 ESS and UES cases, 7 (41%) revealed positivity for AR. Two of three benign stromal nodules were also positive for AR. Moreover, one of the three high-grade sarcomas (undifferentiated endometrial sarcoma) was negative for both ER and PR, but showed positive reaction for AR. In summary, ARs are expressed in 45% of endometrial stromal neoplasms. In addition to determination of ER and PR, the results of immunohistochemical examination of AR in these rare uterine tumors may have some impact on the postoperative management of the patients.     

Primary Fibromatosis of the Breast in a Patient with Multiple Desmoid Tumors: Report of a Case with Evaluationof Estrogen and Progesterone Receptors

Fibromatosis (or desmoid tumor) is an infiltrative fibroblastic/myofibroblastic lesion presenting a moderate risk for local recurrence and no metastatic potential. Classically, these lesions are classified whether in abdominal or extra-abdominal sites, and may be multicentric or familial. Primary fibromatosis of the breast (PFB) is an uncommon lesion that shows histological similarities with abdominal fibromatosis (AF), and frequently poses difficulties in the differential diagnosis with other spindle cell tumors of the breast. It has been demonstrated that AF usually shows immunoreactivity for estrogen (ER) and progesterone (PR) receptors; conversely, in most of the studies, the cells from PFB are consistently negative for both receptors. We report on a case of a 41-year-old female with two desmoid tumors, affecting the abdominal wall and the breast tissue. To the best of our knowledge, there is no previous report in which hormonal receptors were evaluated in abdominal and mammary desmoid tumors in the same patient. We assessed the immunohistochemical expression of ER and PR in both lesions; while the AF showed immunoreactivity for both receptors, the cells from PFB were all negative. Although we have considered just this case, we still believe that these findings could support a distinctive etiopathogenesis of abdominal and mammary fibromatosis.     

Immunolocalization of Androgen Receptor and Estrogen Receptors in Skin Tags

Abstract

Skin tags (STs) are benign connective tissue tumors of the dermis. Several clinical observations suggested the involvement of sex steroids in their development. This study aimed at investigating the possible role of androgen receptor (AR) and estrogen receptors (ERs) in STs pathogenesis through their immunohistochemical (IHC) localization in skin biopsies of this disease and to correlate their expression with different clinical and histopathological parameters. Using IHC techniques, we examined 62 cases with STs and 30 gender- and age-matched, healthy subjects, representing the control group. ERα, ERβ, and AR were upregulated in STs compared to normal skin in epidermis and dermis (p?<?.001 for all). Higher AR H score was significantly associated with axillary STs (p?=?.02), skin colored tags (p?=?.03), acanthosis, and papillomatosis (p?=?.04 for both). Higher ERα H score was significantly associated with hyperpigmented tags (p?<?.001) and positive family history (p?=?.003). Higher ERβ H score was significantly associated with female gender and obesity (p?=?.004 for both). Higher ERα and AR H scores were significantly associated with loose collagen arrangement (p?=?.02, p?=?.004, respectively). Higher AR, ERα, and ERβ H scores were significantly associated with the presence of mast cells (p?=?.01, p?=?.04, p?=?.002, respectively) and dilated blood vessels (p?=?.006, p?=?.04, p?=?.04, respectively). In conclusion, AR and ERs may share in STs pathogenesis through their effect on keratinocytes, fibroblasts, and mast cells.     

Immunohistochemical Demonstration of Cell Proliferation and Estrogen Receptor Status in Human Breast Cancer

Cell proliferation and estrogen receptor (ER) status was investigated in 45 invasive ductal carcinomas of the breast by immunohistochemical methods using monoclonal antibodies Ki 67 (anti-human proliferating cell antibody) and ER ICA. The results were assessed on the basis of nuclear staining intensity and the percentage of positively stained tumor cell nuclei (index score). There was a significant inverse correlation between the Ki 67 and ERICA index scores, although 4 cases showed high index scores for both markers. We conclude that ER positive cells do not always have low proliferation activity, which may be one of the reasons why endocrine therapy is not effective against all ER positive breast cancers. Acta Pathol Jpn 40: 902 907, 1990.     

先天性尿道下裂外生殖器细胞内雄激素受体的表达

本文通过受体的放射配基结合分析，对３０例不同程度的先天性尿道下裂患儿外生殖器细胞的ＡＲ（雄激素受体）进行了测定，实验显示尿道下裂患儿外生殖器细胞核内ＡＲ量减少，表明ＡＲ异常是先天性尿道下裂的原因之一，先天性尿道下裂亦属于雄激素不敏感综合征的范畴。     

乳腺癌合并肥胖症患者血浆瘦素水平与乳腺癌组织中瘦素受体mRNA的表达及临床病理的关系

检测乳腺癌合并肥胖症患者乳腺癌组织中瘦素受体(leptin receptors)mRNA的表达情况和血浆瘦素水平,并探讨二者之间及其与临床病理之间的关系.对124例单纯肥胖者,或乳腺癌合并肥胖者,或乳腺良性病变合并肥胖者同时采集病史、进行体格检查并留取血浆,测定其瘦素的水平.采用半定量逆转录-聚合酶链反应(RT-PCR)检测瘦素受体mRNA的表达.结果显示,乳腺癌合并肥胖组血浆瘦素水平显著高于乳腺良性病变合并肥胖组及单纯肥胖组(P＜0.05).瘦素长型受体[Lep-R(L)]mRNA及瘦素短型受体[Lep-R(S)]mRNA在乳腺癌合并肥胖组中的表达水平均明显高于乳腺良性病变合并肥胖组(P＜0.05).血浆瘦素水平与乳腺组织中Lep-R(L)mRNA及Lep-R(S)mRNA水平呈显著正相关.血浆瘦素和瘦素受体mRNA的表达与腋窝淋巴结转移、绝经、TNM分期及病理类型均无显著相关性(P＞0.05).结论:瘦素可能参与了乳腺癌的发生.     

Secretory Carcinoma Coexistent with Mucinous Carcinoma in the Breast

Secretory carcinoma and mucinous carcinoma were found to coexist in the breast of a 67 year old post menopausal woman, although the tumors were separated by a thin fibrous septum. Histochemically, intra- and extracellular secretory materials in both carcinomas were strongly positive for alcian blue, PAS and mucicarmine staining, but immunohistochemically negative for α- lactalbumin and CEA. Membrane-bound intracytoplasmic vacuoles showing emiocytosis were observed in both the secretory and mucinous carcinomas by electron microscopy. No differences were observed between the tumor cells of secretory carcinoma and those of mucinous carcinoma by histochemistry, immunohistochemistry and electron microscopy. However, there were definite statistically significant differences in the results of morphometry of tumor cell nuclei. Secretory carcinoma is considered to be an anaplastic variant type of mucinous carcinoma. Acta Pathol Jpn 39: 593 598, 1989.     

Estrogen receptors in gastric adenocarcinoma: A retrospective immunohistochemical analysis

Summary Estrogen receptors (ER) in human gastric carcinomas were examined immunohistochemically using a specific monoclonal antibody to human ER. ER-immunoreactivity (ER-IR) was positive in 30 (27.8%) of the 108 gastric carcinomas examined. ER-IR was located in the nucleus of cancer cells. The incidence of ER-IR positive gastric carcinoma was not significantly different between male and female cases. However, the positive tumour cells were observed in 28 (39.4%) out of the 71 poorly differentiated adenocarcinoma, the incidence being significantly higher than that in well differentiated adenocarcinoma (p<0.01). There was no significant difference in the incidence of ER-IR between scirrhous carcinoma and non-scirrhous poorly differentiated adenocarcinoma. Synchronous expression of ER and epidermal growth factor receptor was found in 8 of the 26 scirrhous carcinomas (30.8%). Patients with ER-IR positive scirrhous gastric carcinomas showed a much worse prognosis than those with ER-IR negative scirrhous carcinomas.This work was supported in part by Grant-in Aid for Cancer Research No. 59-15 from the Ministry of Health and Welfare, Japan