小蓟化学成分的分离与鉴定

目的对传统中药小蓟的化学成分进行分离与鉴定,为进一步开发利用小蓟提供科学依据。方法采用反复硅胶柱色谱法进行分离纯化,通过理化常数测定和波谱分析鉴定其化学结构。结果从小蓟提取物的氯仿层和乙酸乙酯层中分离得到9个化合物,即苜蓿素(tricin,1)、蒙花苷(linarin,2)、咖啡酸(caffeic acid,3)、香豆酸(courmaric acid,4)、丁二酸(succinic acid,5)、芹菜素(apigenin,6)、苜蓿素-7-O-β-D-葡萄糖(tricin-7-O-β-D-glucopyranoside,7)、β-谷甾醇(β-sitosterol,8)、β-胡萝卜苷(β-daucosterol,9)。结论化合物5、7为首次从蓟属植物中分离得到,化合物1、4为首次从小蓟中分离得到。     

Cytotoxic triterpenoids from Berberis koreana

A bioassay-guided fractionation and chemical investigation of the trunk of Berberis koreana led to the isolation and identification of three new triterpenoids, 2 α,3 α,19 α-trihydroxy-urs-12-en-24-formyl-28-oic acid (1), 2 α,3 β,21 α-trihydroxy-urs-12-en-28-oic acid ( 2), and 3 β-acetyloxy-1-oxo-olean-12-en-28-oic acid (3), along with seven known triterpenoids (4-10). The structures of these new compounds were determined through spectral analysis, including extensive 2D-NMR data. The new compounds 1-3 showed significant cytotoxicity against the A549, SK-OV-3, SK-MEL-2, and HCT-15 human tumor cell lines with IC(50) values ranging from 7.17 to 48.73?μM.     

白蔹的化学成分

目的研究白蔹的化学成分。方法利用各种色谱法进行分离,根据理化性质和波谱数据鉴定结构。结果从白蔹的乙酸乙酯萃取液中分离得到8个已知成分,分别鉴定为没食子酸(1)、原儿茶酸(2)、龙胆酸(3)、白藜芦醇(4)、大黄素(5)、羽扇豆醇(6)、β-谷甾醇(7)、胡萝卜苷(8)。结论化合物2和3为从该植物中首次分离得到。     

锁阳中三萜及甾体成分的研究

From the whole parasitic plant of Cynomorium songaricum Rupr. three ursane type triterpenes, three steroidal compounds, palmitic acid and sucrose were isolated. The triterpenes were identified as acetyl ursolic acid (2), ursolic acid (3) and a new compound, ursa-12-ene-28-oic acid, 3β-propanedioic acid monoester (1). The steroidal compounds were identified as β-sitosterol palmitate (4), β-sitosterol (5) and β-sitosterol glucoside (daucosterol, 6). Compounds 2 and 5 were found in this genus for the first time.     

Cytotoxic triterpenoids and steroids from the bark of Melia azedarach

Two new triterpenoids (1, 2) and two new steroids (3, 4) along with twelve related known compounds (5-16) were isolated from the bark of Melia azedarach. The new structures were elucidated by means of spectroscopic methods and molecular modeling studies and found to be 21,24-cycloeupha-7-ene-3 β,16 β,21 α,25-tetrol (1), 3 β-acetoxy-12 β-hydroxy-eupha-7,24-dien-21,16 β-olide (2), 29-hydroperoxy-stigmasta-7,24(28) E-dien-3 β-ol (3), and 24 ξ-hydroperoxy-24-vinyl-lathosterol (4). All isolated compounds were tested for their cytotoxic activity against three human cancer cell lines (A549, H460, HGC27) using the CellTiter Glo? luminescent cell viability assay. Among them, compounds 2- 4, 24 ξ-hydroperoxy-24-vinyl-cholesterol (6), kulinone (7), meliastatin 3 ( 8), 3-oxo-olean-12-en-28-oic acid (10), and (22 E,24 S)-5 α,8 α-epidioxy-24-methyl-cholesta-6,22-dien-3 β-ol (12) were found to have cytotoxic effects, with IC?? values of 5.6-21.2?μg/mL.     

A new eudesmane sesquiterpene glycosides from Liriope muscari

A new eudesmane sesquiterpene glycoside, ophiopogonoside B (1), along with five known compounds, ophiopogonoside A (2), ruscogenin-1-O-[β-D-glucopyranosyl (1 → 2)]-[β-D-xylopyranosyl (1 → 3)]-β-D-fucopyranoside (3), palmitic acid (4), palmitic acid glyceride (5), and β-sitosterol-D-glucopyranoside (6),was isolated from the tuberous roots of Liriope muscari (Decn.) Bailey (Liliaceae). Their structures were confirmed by 1D and 2D NMR spectroscopy. Among them, compounds 1, 2, 4, and 5 were the first reported from the genus Liriope. Ophiopogonoside B (1) showed moderate inhibitory activity to glycogen phosphorylase a.     

Metabolite profiling of the leaves of the Brazilian folk medicine Sideroxylon obtusifolium

Sideroxylon obtusifolium (Roem. & Schult.) T. D. Penn. (family Sapotaceae) is a tree native to Central and South America. Infusions of the bark and the leaves are used in Brazilian folk medicine as an anti-inflammatory remedy. However, information on the constituents of S. obtusifolium remains scarce, and only common pentacyclic triterpenoids have been previously reported. HPLC-DAD/MS analyses revealed that saponins and flavonoids were the main constituents of the leaves. From the butanol-soluble fraction of an ethanolic extract, a total of four saponins and ten flavonol glycosides were isolated by a combination of chromatographic methods including Sephadex LH-20, MPLC, and HPLC. Their structures were established by acid hydrolysis and spectroscopic methods, mainly MS (n), 1D and 2D NMR experiments. The compounds include the new triterpene glycoside 3-O-( β-D-glucopyranosyl)-protobassic acid 28-O- β-D-apiofuranosyl-(1 → 3)-O-[O- β-D-apiofuranosyl-(1 → 3)- β-D-xylopyranosyl-(1 → 4)]-O- α-L-rhamnopyranosyl-(1 → 2)- α-L-arabinopyranosyl ester ( 1), as well as the new flavonol glycosides, quercetin-3-O-(O- α-L-rhamnopyranosyl-(1→ 2)-O-[ β-D-glucopyranosyl-(1 → 3)]- β-D-galactopyranoside) ( 6) and kaempferol-3-O-(O- α-L-rhamnopyranosyl-(1 → 2)-O-[ β-D-glucopyranosyl-(1 → 3)]- β-D-galactopyranoside) ( 8). In addition, catechin and a glycerogalactolipid, gingerglycolipid A, were obtained from the ethyl acetate-soluble fraction. The isolated compounds could be used in the future as chemical markers for quality control of this herbal drug.     

Three new diterpenoids from the leaves of Thuja orientalis

Three new diterpenoids, 18-formyloxy-8 β-hydroxysandaracopimar-15-ene (1), 15(R)-N-butoxypinusolidic acid (2), and 15,16-dihydro-15,16-dimethoxylambertianic acid (3), along with twelve known compounds (4-15) were isolated from MeOH extracts of leaves of Thuja orientalis L. The structures of the three new compounds were elucidated on the basis of spectroscopic analyses, including extensive 2D-NMR data. The absolute stereochemistry of compound 2 was clarified by a CD spectroscopic study. The isolated compounds were evaluated for cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT-15 cell lines in vitro using the sulforhodamin B (SRB) bioassay.     

Two new triterpenoid glycosides from the leaves of Anthocephalus chinensis

Nine compounds were isolated from the leaves of Anthocephalus chinensis by column chromatography on silica gel and Sephadex LH-20, and their structures were elucidated by spectroscopic techniques as clethric acid-28-O-β-d-glucopyranosyl ester (1), mussaendoside T (2), β-stigmasterol (3), hederagenin (4), ursolic acid (5), clethric acid (6), 3β,6β,19α,24-tetrahydroxyurs-12-en-28-oic acid (7), mussaendoside I (8), and cadambine (9). Compounds 1 and 2, and 7 and 8 were isolated from the plants of this genus for the first time, and compounds 1 and 2 were new triterpenoid glycosides.     

Ozocardic A: a new alkylanacardic acid from Ozoroa pulcherrima

One new alkylanacardic acid, ozocardic A (1), along with the known and related metabolites 6-tridecyl anacardic acid (2) and β-sitosterol (3) was isolated from Ozoroa pulcherrima. The structure of the new compound was elucidated by detailed spectroscopic analysis such as (1)H, (13)C NMR, COSY, HMQC, HMBC, and HREIMS. The structures of known compounds (6-tridecyl anacardic acid (2) and β-sitosterol (3)) were identified by the comparison of their spectral data with those published in the literature.     

平卧菊三七叶化学成分的分离与鉴定

目的研究平卧菊三七叶子的化学成分。方法采用硅胶柱色谱法、反相ODS柱色谱法、Seph-adex LH-20柱色谱法和HPLC法等进行分离纯化,并通过波谱分析技术鉴定其化学结构。结果从平卧菊三七叶子中分离得到7个化合物,分别鉴定为β-谷甾醇-3-O-龙胆二糖苷(β-sitosterol-3-O-gentiobioside,1)、3-吲哚甲酸(indole-3-carboxylic acid,2)、蚱蜢酮(grasshopper ketone,3)、β-谷甾醇(β-sitosterol,4)、毒豆甲酮(drummondone A,5)、正三十二烷醇(n-dotriacontanol,6)和毒豆乙酮(drummondone B,7)。结论化合物1～3、5、7为首次从菊三七属植物中分离得到,化合物4、6为首次从平卧菊三七中分离得到。     

天师栗化学成分的研究

目的：从中药天师栗(Aesculus wilsonii Rehd.)的干燥成熟果实中寻找新的有效成分。方法：利用各种色谱技术进行纯化,根据理化性质和各种光谱技术(IR,EIMS,FABMS,¹HNMR,¹³CNMR,¹H-¹H COSY,¹³C-¹H COSY)进行结构鉴定。结果：从天师栗的95%乙醇浸膏的乙酸乙酯和己烷部分共分得5个化合物。分别鉴定为：天师酸(1),富马酸(2),乙酰谷氨酸(3),β-谷甾醇-3-O-葡糖苷(4),β-谷甾醇(5)。结论：化合物1为新化合物,2和3是从本科植物首次分得。     

白枪杆化学成分的研究[Ⅱ]

目的 研究木犀科梣属植物白枪杆的化学成分.方法 将2 000株幼苗以溶剂提取法和萃取法将成分分为水溶性和脂溶性成分,再以色谱分离法分离得化合物,最后经质谱、核磁共振等光谱方法确认化合物结构.结果 分离得到10个化合物,并对其进行了结构鉴定.结论 水层各个化合物的结构被鉴定为:红景天苷(1)、3,4-二羟基苯乙醇苷(2)、3,4-二羟基苯乙醇(3)、对羟基苯乙醇(4)、4-甲氧基水杨酸(5);氯仿层各个化合物的结构被鉴定为:齐墩果酸(6)、水杨酸( 7)、香草酸(8)、　β-谷甾醇(9)、β-胡萝卜苷(10).     

草苁蓉化学成分的研究

目的：研究我国民间草药草苁蓉［Boschniakia rossica (Cham.et Schltdl) Fedtsch.et Flerov.］的化学成分。方法：草苁蓉全草醇提取物的乙酸乙酯部位经硅胶柱色谱和制备薄层分离,分得8个化合物。通过波谱和化学方法鉴定了它们的结构。结果：8个化合物的结构分别鉴定为4,5,6-三羟基噢酮(1)、β-谷甾醇(2)、β-谷甾醇3-O-β-D-葡糖苷(3)、7-去氧-8-表马钱子酸(4)、没食子酸(5)、肉桂酸(6)、咖啡酸(7)和草苁蓉纳拉苷(8)。结论：4,5,6-三羟基噢酮(1)为新的噢酮化合物,4个已知化合物:7-去氧-8-表马钱子酸(4)、没食子酸(5)、肉桂酸(6)、咖啡酸(7)为首次从草苁蓉中分得。     

A new flavonol glucoside from the aerial parts of Sida glutinosa

Phytochemical investigation on the dried aerial parts of Sida glutinosa has led to the isolation of a new flavonol glucoside, glutinoside (1), along with seven known compounds, 24(28)-dehydromakisterone A (2), 1,2,3,9-tetrahydropyrrolo[2,1-b]-quinazolin-3-amine (3), docosanoic acid, 1-triacontanol, campesterol, stigmasterol, and β-sitosterol. The structures of these compounds were elucidated by means of extensive spectroscopic techniques as well as GC/MS analysis (for sterols) and comparison with the literature data. All these seven known compounds are reported from this plant for the first time.     

珠子参的化学成分

目的对珠子参[PanaxjaponicusC.A.Mey.var.major(Buck.)C.Y.Wu et K.M.Feng]根茎的化学成分进行研究。方法采用硅胶柱色谱进行分离纯化,根据理化性质和波谱数据进行结构鉴定。结果分离得到8个化合物,分别鉴定为苯甲酸(benzoin acid,1)、豆甾醇(stigmasterol,2)、齐墩果酸(oleanolic acid,3)、胡萝卜苷(daucosterol,4)、齐墩果酸-28-O-β-D-吡喃葡萄糖苷(oleanolicacid-28-O-β-D-glucopyranoside,5)、齐墩果酸-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-(6′-丁酯)吡喃葡萄糖醛酸苷(oleanolic acid-3-O-β-D-glucopyranoside(1→2)-β-D-glucorunopyranosy-6′-O-butylester,6)、竹节参皂苷IVa(chikusetsusaponinⅣa,7)、人参皂苷Ro(ginsenoside,8)。结论化合物6为新化合物,化合物1、2为首次从该种植物中分离得到。     

岗梅叶的化学成分

目的对岗梅叶的化学成分进行研究。方法采用反复硅胶柱色谱、凝胶柱色谱进行分离?纯化,并通过其理化性质,结合光谱数据鉴定其化学结构。结果分离得到9个化合物,分别鉴定为:rotundioic acid(1)、2α,3β,19α-三羟基乌索-12-烯-23,28-二羧酸(2)、2α,3β,19α-三羟基齐墩果-12-烯-23,28-二羧酸(3)、山柰酚(4)、山柰酚-3-O-β-D-葡萄糖苷(5)、2(E)-2,6-二甲基-2,7-辛二烯-6-羟基-1-醇苷(6)、2,3-二羟基苯甲酸(7)、β-谷甾醇(8)、胡萝卜苷(9)。结论化合物1-7为从该种植物中首次分离得到。     

人参化学成分的研究——12.人参脂溶性成分的研究

鲜人参根(Panax ginseng C.A.Meyer)的醚溶性部分有较强的抗炎作用。从该部分中用砖胶低压柱层析,分得9种单体,经UV、IR、MS及GC等方法鉴定了7种化学结构,分别为二十九烷(1)、三棕榈酸甘油脂(2)、人参萜醇(4)、三亚油酸甘油酯(5)、棕榈酸(6)、β-谷甾醇(7)、α,γ-二棕榈酸甘油酯(9)。其中化合物(1)、(2)、(5)、(9)是首次从人参中分离出的已知成分、化合物(4)系首次在国内分离得到的已知成分。     

白花蛇舌草的化学成分

目的对中药白花蛇舌草(Hedyotis diffusaWilld.)化学成分进行分离鉴定。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱、制备薄层色谱、重结晶等方法进行分离纯化,并通过理化常数测定和光谱数据分析鉴定其化学结构。结果分离得到7个化合物,分别鉴定为β-谷甾醇(β-sitosterol,1)、胡萝卜苷(daucosterol,2)、熊果酸(ursolic acid,3)、2-羟基-3-甲基-1-甲氧基蒽醌(2-hydroxy-3-methyl-1-methoxyanthraquinone,4)、2-羟基-7-甲基-3-甲氧基蒽醌(2-hydroxy-7-methyl-3-methoxyanthraquinone,5)、E-6-O-对甲氧基肉桂酰基鸡屎藤苷甲酯(E-6-O-p-methoxycinnamoyl scandoside methyl ester,6)、E-6-O-香豆酰基鸡屎藤苷甲酯(E-6-O-p-coumaroyl scandoside methyl ester,7)。结论化合物5为首次从该属植物中分离得到。     

苍耳子的化学成分

目的研究苍耳子的化学成分。方法应用多种色谱方法进行分离和纯化,并利用NMR和MS等方法解析化合物结构。结果从苍耳子的乙酸乙酯提取物中分离得到7个化合物,它们的结构分别被鉴定为:β-谷甾醇(1)、胡萝卜苷(2)、丁二酸(3)、阿魏酸(4)、3-甲氧基-4-羟基桂皮醛(5)、咖啡酸(6)、5-O-咖啡酰奎宁酸甲酯(7)。结论化合物5和7为首次从该植物中分离得到。