复合酶中糖化酶活力的测定

复合糖化酶的酶活力检测是主要的质量控制指标,只有选用合理的糖化酶活力检测方法,才能真实反映出复合酶中糖化酶活力的高低.为避免因协同作用而产生的复合酶中其他酶制剂对糖化酶活力检测的干扰,作者总结了以往的研究成果,结合4种不同酶制剂的作用机理,最终否定了常用的淀粉底物法,确定了麦芽糖底物法为首选方法.     

转苷酶活性测定方法

对测定糖化酶中葡萄糖苷转移酶（转苷酶）酶活的方法进行了研究。采用α－ＭＧ为底物，用ｋｎｉｇｈｔ－Ａｌｅｎ法测定转苷酶催化α－ＭＧ分解产生的葡萄糖，计算得转苷酶酶活值。通过条件试验，找到了底物浓度、碱性铜试剂用量、加热时间、滴定剂浓度等的最佳范围。并测定了３×１０４，５×１０４，６×１０４μｍｏｌ／ｍｉｎ糖化酶粉剂和发酵液中的转苷酶活性，做了酶法、光度法与该方法的对比试验。     

A Statistical Comparison of Three Methods for the Counting of Human Spermatozoa

Three methods for the counting of human spermatozoa were compared and evaluated. Firstly the white blood cell pipette and hemocytometer method was used. For the second method the Makler counting chamber was used and the results of the third method were obtained by using a glass tuberculin syringe instead of the white blood cell pipette for diluting purposes. The tuberculin syringe is the standard method used in this laboratory. The tuberculin method showed a high degree of precision and accuracy, especially when compared to the makler counting chamber method. Through a simultaneous pairwise linear structural relationships analysis, whereby an error variance was estimated for each method, it was found that the tuberculin syringe method was the most accurate followed by Makler's method, and then the white blood cell pipette method.     

三种食管测压方法的对比研究：附15例正常人的结果分析

本文报告灌注测压法，微型传感器测压法及气囊测压法三种方法测定１５例正常人食管各部位压力的结果。结果显示：除灌注法与微型传感器测压法所测食管末端括约肌压值组间比较有显著性差异外（Ｐ＜０．０５），其余组间无显著性差异（Ｐ＞０．０５）。说明三种方法均可使用于临床。但灌注测压法最佳，病人相对较舒适，准确性高。微型传感器测压法昂贵。而气囊测压法准确性相对不稳定。     

两种精浆酸性磷酸酶检测方法的比较与评价

目的:对检测精浆酸性磷酸酶(ACP)活性的常规方法(磷酸苯二钠法)和试剂盒方法进行比较,并探讨试剂盒方法取代常规方法用于常规检测的可能性。方法:79份不育男性的精浆,分别用常规方法和试剂盒方法检测其ACP活性。取1份精浆标本用于两种方法的批内检测,另取2份标本用于两种方法的批间检测。随机留取10份精浆标本,稀释后立即或放置30 m in后检测ACP活性。另随机留取10份精浆标本,同时由2位技术人员分别用常规方法检测ACP活性。结果:试剂盒方法所测ACP活性与常规方法显著相关(r=0.745,P=0.000)。试剂盒方法与常规方法相比,两者批内CV(13.72%;10.66%)比较接近,而批间CV(13.8%和15.49%;24.43%和21.04%)明显较低。精浆稀释后放置30 m in,无论是常规方法还是试剂盒方法,所测结果都显著降低(P<0.05)。而2位技术人员用常规方法所测精浆ACP活性无显著区别(P=0.165)。结论:试剂盒方法检测精浆ACP活性优于常规方法,可以取代常规方法。     

Comparison of Three Different Surgical Methods in Aortic Root Aneurysms: Long-Term Results

Abstract Background: Degenerative ascending aortic aneurysms frequently present with aortic valve pathology. If only the noncoronary sinus of Valsalva is dilated, replacement of the noncoronary sinus by tailoring the supracoronary graft with or without aortic valve replacement (AVR) can be a simple operation strategy. We sought to describe our experiences in this kind of operation, and compare them with a relatively homogeneous group of patients who underwent the classical Bentall operation. Methods: Between January 1997 and June 2007, 99 patients who had dilated ascending aortas with root dilatation and aortic valve pathology underwent operation. Patients were divided into three groups according to the surgical procedure. Classical Bentall operation was applied in 54 patients with dilated sinuses (Group 1). The other patients with dilated noncoronary sinus underwent either ascending aortic replacement with noncoronary sinus replacement (NSR, n = 27) (Group 2), or separate AVR and ascending aortic replacement with NSR (AVR + NSR, n = 18) (Group 3). Results: There were significant reduction of aortic root in Group 2 (Z =?4.560, p < 0.001), and Group 3 (Z =?3.758, p < 0.001). Degree of aortic regurgitation was decreased from 1.56 ± 0.5 to 0.67 ± 0.5 postoperatively in Group 2 (Z =?3.874, p < 0.001). Hospital mortality was six (6.1%) (three in Group 1; three in Group 2). Late mortality rate was 6.1% (three in Group 1, three in Group 2). The type of operation was not found to be an independent predictor of overall mortality. No patients in the NSR and AVR‐NSR groups had aortic root aneurysm, and there were no reoperations or verified deaths caused by aortic root aneurysm in these patients. Conclusions: Noncoronary cusp replacement for aortic root remodeling in patients with ascending aortic aneurysm is a technically simple and durable operation. Short‐ and mid‐term results of this method were favorable compared to the Bentall procedure.     

国产垦啤-2号大麦制麦酶系的影响因素

以垦啤 2号大麦(适合东北气候、土质种植)为原料,将酶活分析方法与传统制麦方法相结合,按照最佳制麦工艺所得成品麦芽各项指标均介于一级品与优级品之间,其中,糖化力、糖化时间、α 氨基氮、可溶性氮均优于优级品.通过研究制麦过程中各种添加剂对绿麦芽酶活的影响表明,利用体积分数0.1%甲醛浸麦1h效果较好;同样条件下,较质量分数0.9%NaOH浸麦,制麦酶系都有所提高.     

3种镇痛方法用于人工流产术镇痛效果比较

目的 探讨无痛人工流产术的有效性、安全性及实用性。方法 将250例人工流产者随机分为A组（80例）、B组（92例）、C组（78例）。A组采用异丙酚静脉麻醉，B组采用氧化亚氮（50％氧化亚氮与50％氧气混合气体）吸入，C组进行利多卡因宫颈局麻。观察3组人工流产术中镇痛效果、生命体征变化、宫颈口松弛情况、术中出血、人工流产综合征（RAAS）发生率及手术时间。结果 A组镇痛有效率为100．0％，宫颈松弛有效率为91．2％，显著高于B、C组（P〈0．05、P〈0．01），且无RAAS发生，但术中易发生一过性血压、心率降低。3组术中出血、手术时间比较，差异无显著性意义（均P〉0．05）。结论 3种镇痛方法均安全有效，其中异丙酚能达到完全镇痛效果，使人工流产更具有人性化，利于提高育龄妇女的生活质量。     

3种镇痛方法用于人工流产术镇痛效果比较

目的 探讨无痛人工流产术的有效性、安全性及实用性.方法 将250例人工流产者随机分为A组(80例)、B组(92例)、C组(78例).A组采用异丙酚静脉麻醉,B组采用氧化亚氮(50%氧化亚氮与50%氧气混合气体)吸入,C组进行利多卡因宫颈局麻.观察3组人工流产术中镇痛效果、生命体征变化、宫颈口松弛情况、术中出血、人工流产综合征(RAAS)发生率及手术时间.结果 A组镇痛有效率为100.0%,宫颈松弛有效率为91.2%,显著高于B、C组(P＜0.05、P＜0.01),且无RAAS发生,但术中易发生一过性血压、心率降低.3组术中出血、手术时间比较,差异无显著性意义(均P＞0.05).结论 3种镇痛方法均安全有效,其中异丙酚能达到完全镇痛效果,使人工流产更具有人性化,利于提高育龄妇女的生活质量.     

Comparison of Three Hypotensive Anaesthetic Methods for Middle Ear Microsurgery

Three hypotensive anaesthetic methods were compared in 123 patients undergoing middle ear microsurgery. Hypotension was induced with halothane (1 vol.%), nitroprusside (4.4 micrograms/kg/min) or the combination of halothane (0.5 vol.%) and nitroprusside (1.2 micrograms/kg/min). The compensatory rise in the heart rate was controlled with a beta-adrenergic receptor blocking agent, practolol, the mean need for which was 0.03 mg/kg in the halothane group and 0.1 mg/kg in the other groups. The mean duration of the hypotensive period ranged from 79 to 107 min in the groups. During the hypotension, the average mean arterial pressure ranged from 55 to 60 mmHg (7.33 to 8.00 kPa) and the mean heart rate from 67 to 79 beats/min in the groups. The PaO2 value in the nitroprusside and halothane + nitroprusside groups, but not in the halothane group, decreased statistically and in some cases clinically significantly. The only peroperative side effects were ECG changes (mostly junctional rhythm), the incidence of which ranged from 16 to 24% in the groups. The mean values for the degree of haemostasis rated by the otologist on a visual analogue scale between poor (0 mm) and excellent (100 mm) were 90, 73 and 89 mm in the halothane, nitroprusside and halothane + nitroprusside groups, respectively. Recovery in the halothane group was statistically highly significantly longer than in the other groups. No rebound hypertension occurred in any of the groups after discontinuation of the hypotension. The most common side effect was nausea + vomiting, the incidence of which ranged from 15 to 26% in the groups.(ABSTRACT TRUNCATED AT 250 WORDS)