耐高渗酵母产赤藓糖醇的影响因素

球拟酵母OS-194是一株单产赤藓糖醇的耐高渗酵母,该菌株高产赤藓糖醇的最佳培养基配方为葡萄糖10g/dL,酵母膏0.5g/dL,尿素0.1g/dL.最适培养条件是在摇瓶转速150r/min的条件下于35℃培养4d.在上述培养条件下，该菌株赤藓糖醇的耗糖转化率高达29.6%.磷是限制OS-194菌株高产赤藓糖醇的主要因素,当培养液中的磷质量浓度低于31.5mg/L时，赤藓糖醇的产量最高;随着磷质量浓度的升高，该菌株赤藓糖醇的产量降低,而酒精的产量和生物量却有明显升高.同时,OS-194菌株还能利用果糖、蔗糖和D-甘露糖产赤藓糖醇.     

耐高渗酿酒酵母的代谢流分析

研究了指数生长期的酿酒酵母 (Saccharomyces cerevisiae) 在高渗胁迫下的生理代谢.甘油和海藻糖是酿酒酵母的主要相容性溶质.在高渗环境下,胞外酒精质量浓度、甘油质量浓度、海藻糖质量浓度分别较对照提高100%、400%和11%.代谢流分析表明,高渗环境下,从节点6-磷酸葡萄糖合成海藻糖的碳流较常规培养增加了47.3%;从节点磷酸二羟丙酮和3-磷酸甘油醛合成甘油的碳流增加了55.7%;从节点丙酮酸流向乙醇的碳流增加了300%,而流向三羧酸 (TCA) 循环的流量减少了21.7%.     

高渗葡萄糖注射液湿敷治疗局部封闭感染创面

自1988年3月至今，应用高渗葡萄糖注射液湿敷治疗局部封闭感染创面35例，获得满意疗效。临床资料本组35例中男22例，女13例；年龄27～65岁；创面面积6cmX4cm～3Oc。。。Xscm；创面愈合时间平均275天。治疗方法局部封闭感染经切开后先用双氧水和生理盐水清洗分泌物，并清除坏死组织，然后取无菌纱布3～4层，用50％葡萄糖注射液适量浸湿无菌纱布，然后覆盖在创面上，用无菌绷带包扎。以后每12小时给创面浇50％葡萄糖注射液1次，每2日换药1次。讨论局部封闭感染创面以组织水肿坏死为主，分泌物培养多未见细菌生长。高渗葡萄糖具有抑制细菌生…     

猕猴桃汁中产香酵母的分离、鉴定及生长特性

从优质自然发酵猕猴桃汁中分离筛选到一株产香酵母E 36 ,经鉴定为膜醭假丝酵母Can didapelliculosa .研究了该菌在麦芽汁中的生长特性 ,发现其最适生长温度为 32℃ ,最低和最高生长温度分别为 9℃和 4 1.5℃ ;最适生长 pH为 5.4 ,最低和最高生长 pH分别为 2 .0和 12 .0 .     

法夫酵母产虾青素的补料发酵

以法夫酵母 (Phaffiarhodozyma)WSS FF6为产生菌进行产虾青素的补料发酵 ,在通气量为 2 5 0L/h、pH =6.0± 0 .5的条件下 ,先流加高糖浓度的培养基 ,后添加 0 .1%乙醇 ,进行分批补料发酵 ,经 130h发酵后 ,生物量与类胡萝卜素产量分别为 2 7.4mg/mL、2 6.12 μg/mL ,生长得率、产物得率及酵母色素质量分数分别为 0 .4 6、0 .4 4和 0 .95     

高渗氯化钠溶液对红细胞的影响

关于高渗氯化钠溶液(HS)的研究报道,从基础和临床上充分肯定了小剂量HS明显的抗休克作用。此类文献多将研究焦点放在HS对血流动力学的影响上。红细胞是血液中含量最多的有形成分,其功能状态影响休克的转归。本文从细胞生物学、生物化学及血液流变学的角度,综述了HS对红细胞的影响。     

葡萄糖对光滑球拟酵母发酵生产丙酮酸的影响

在30L发酵罐中研究了初始葡萄糖质量浓度和补料方式对光滑球拟酵母WSH-IP303发酵生产丙酮酸的影响．实验确定116.4g/L左右是较为适宜的初始葡萄糖质量浓度,发酵58h时丙酮酸质量浓度和产率分别为58.0g/L和0.516g/g．采用初始葡萄糖质量浓度为53.4g/L,发酵24h分批补料至葡萄糖总质量浓度为115g/L的培养方式,发酵64h时丙酮酸质量浓度和产率分别为60.2g/L和0.559g/g;采用初始葡萄糖质量浓度为62.6g/L,发酵24h开始连续补料至葡萄糖总质量浓度为115g/L的培养方式,发酵72h时丙酮酸质量浓度和产率分别为63.3g/L和0.586g/g,与葡萄糖总质量浓度相似(115g/L)的分批发酵相比,丙酮酸产量分别提高了3.8%和9.1%．实验结果表明适宜的初始葡萄糖质量浓度能促进光滑球拟酵母发酵生产丙酮酸;尽管葡萄糖补料培养可适度提高丙酮酸的产量及产率,但生产强度却有所下降．     

法夫酵母产虾青素的摇瓶工艺

以法夫酵母 (Phaffiarhodozyma)WSS -FF6为产生菌进行摇瓶条件下的类胡萝卜素 (主要为虾青素 )发酵条件研究 .正交试验表明 :葡萄糖质量分数对酵母产色素影响较大 ,在起始 pH值为 6,培养温度 2 2℃ ,葡萄糖 3 .0 %、尿素 0 .1 %、磷酸二氢钾 0 .6%、玉米浆 0 .6%的培养基下经过72h、2 2 0r/min摇瓶发酵 ,其生物量为 6.58mg/mL ,类胡萝卜素产量为 1 4.92 μg/mL ,其中虾青素占 78% .     

胶质瘤细胞的生长速度与葡萄糖摄取的关系

目的　探讨胶质瘤细胞的生长速度与葡萄糖转运系统的关系。方法　用C6细胞克隆培养得到不同细胞形态、不同生长速率的细胞株。Northernblot和Westernblot检测生长最慢的C61细胞和最快的C64 细胞葡萄糖转运体 1(GLUT1)mRNA表达及其蛋白水平 ;同时检测其葡萄糖摄取率。结果　C6胶质瘤细胞克隆培养产生C61、C62 、C63 、C64 4种细胞 ,4种细胞的生长速度不同 ,C64 细胞的生长速度约为C61细胞的 8倍。C6胶质瘤细胞的克隆细胞株主要表达GLUT1,C64 细胞比C61细胞的GLUT1mRNA水平明显增加 ,条带的吸光度分析显示C64 细胞的GLUT1蛋白水平约为C61细胞的 3倍 [(3 10± 2 1) % ,P <0 .0 0 1]。葡萄糖摄取率以每分钟液闪次数计 :C64 细胞 (7680± 180 ) /min·10 4cells ;C61细胞 (3 0 0 0± 12 5 ) /min·10 4cells ,C64 细胞约为C61细胞的2 .5倍。结论　葡萄糖转运系统与葡萄糖摄取相匹配 ,与胶质瘤细胞的生长密切相关。     

加温稀释碘伏和高渗葡萄糖联合微创胸膜粘连术治疗顽固性自发性气胸25例

目的:探讨胸部微创术后用温稀释碘伏和高渗葡萄糖联合治疗顽固性自发性气胸的效果。方法:25例自发性气胸经肺大疱切除后,加温热稀碘伏水保留0.5h,吸尽后加入50%高渗葡萄糖60ml均匀喷洒脏壁层胸膜,0.5h时开放引流。结果:本组患者总有效率100%,随访未见气胸复发。结论:胸部微创、加温稀释碘伏水和高渗葡萄糖联合胸膜粘连术治疗顽固性自发性气胸安全、有效、价廉,值得推广。     

Scoliosis and Growth: Patterns of Asymmetry in Normal Vertebral Growth

This study reviews published observations of asymmetrical appearance of primary ossification centres in human fetal vertebral arches. It reports studies of vertebral asymmetry in 39 vertebral columns of infants and children including asymmetry in pedicle length and vertebral arch height, asymmetry in neurocentral fusion, and vertebral body flattening on its left anterior aspect. It relates these patterns of asymmetry to the commonly observed left thoracic scoliosis of infancy and right thoracic scoliosis of adolescents and adults. It discusses the implications of these observed asymmetries of normal vertebral growth in the aetiology of scoliosis, and the possible influences of handedness and aortic pressure in the production of these vertebral asymmetries in adolescence.     

Scoliosis and Growth: Patterns of Asymmetry in Normal Vertebral Growth

This study reviews published observations of asymmetrical appearance of primary ossification centres in human fetal vertebral arches. It reports studies of vertebral asymmetry in 39 vertebral columns of infants and children including asymmetry in pedicle length and vertebral arch height, asymmetry in neurocentral fusion, and vertebral body flattening on its left anterior aspect. It relates these patterns of asymmetry to the commonly observed left thoracic scoliosis of infancy and right thoracic scoliosis of adolescents and adults. It discusses the implications of these observed asymmetries of normal vertebral growth in the aetiology of scoliosis, and the possible influences of handedness and aortic pressure in the production of these vertebral asymmetries in adolescence.     

Linear Growth Patterns in Prepubertal Children Following Liver Transplantation

Factors impacting linear growth following pediatric liver transplantation (LT) are not well understood. This longitudinal analysis examines predictors of linear growth impairment in prepubertal children included in Studies of Pediatric Liver Transplantation. In 1143 children with serial measurements, mean height scores increased from −1.55 at LT to −0.87 and −0.68 at 24 and 36 months post LT with minimal subsequent catch up growth observed until 60 months. Subgroup analysis of height measurements at 24 months (n = 696), 33.8% were below 10th percentile at 24 months post LT. Multivariate analysis revealed linear growth impairment more likely in patients with metabolic disease (OR 4.4, CI: 1.83–10.59) and >18 months of steroids exposure (OR 3.02, CI: 1.39–6.55). Higher percentiles for weight (OR 0.80, CI: 0.65–0.99) and height (OR 0.62, CI: 0.51–0.77) at LT decreased risk. Less linear catch up was observed in patients with metabolic disease, non-Biliary atresia cholestatic diseases and lower weight and higher height percentiles prior to LT. Prolonged steroid exposure and elevated calculated glomerular filtration rate and γ-Glutamyltransferase following LT were associated with less catch up growth. Linear growth impairment and incomplete linear catch up growth are common following LT and may improve by avoiding advanced growth failure before LT and steroid exposure minimization.     

VSD技术联用表皮生长因子对深度烧伤创面愈合及炎症应激反应的影响

探讨VSD技术联用表皮生长因子对深度烧伤创面愈合及炎症应激反应的影响。方法 选取 2022年11月-2023年11月我院收治的深度烧伤患者62例,随机分为对照组和观察组,各31例。对照组应 用VSD技术与生理盐水冲洗治疗,观察组在对照组基础上应用表皮生长因子治疗,比较两组创面愈合 情况和炎症应激反应情况。结果 观察组创面愈合时间短于对照组,瘢痕生成数及感染数低于对照组 （P＜0.05）;观察组IL-1、TNF-α、E、NE、R水平低于对照组（P＜0.05）。结论 VSD技术结合表皮生 长因子能促进深度烧伤患者的创面愈合,提高创面愈合效果,减轻患者炎症应激反应。     

Renin, Growth Hormone and Insulin in Hemodialyzed Uremic Diabetics

In order to detect metabolic aberrations which could account for the poor therapeutic response of the diabetic to dialysis, a comparative study of nondiabetic and diabetic uremia patients treated with hemodialysis was undertaken. Plasma renin activity and levels of growth hormone, glucose and insulin were investigated.     

胰岛素样生长因子1受体高表达提示直肠癌放射治疗耐受

目的 研究胰岛素样生长因子1受体(IGF-1R)表达水平与直肠癌放射治疗(简称放疗)敏感性的关系.方法 选择江苏省中医院消化肿瘤外科2009年1月至2010年12月期间的87例直肠癌患者,应用免疫组织化学和逆转录聚合酶链反应( RT-PCR)方法检测直肠癌放疗前活检标本及相应的术后标本中IGF-1R蛋白和mRNA的表达...     

Perfectionism Affects Blood Pressure in Response to Repeated Exposure to Stress

This pilot study examined the effects of perfectionism on blood pressure (BP) in response to repeated exposure to mental arithmetic stressors. College students (N = 30) in a laboratory setting were administered a series of challenging mathematical tasks. BP was measured at baseline and after each task. Multilevel modelling analyses revealed that BP tended to decline over the course of the mathematical tasks. However, higher levels of performance standards predicted relatively stable levels of systolic BP, whereas moderate and lower levels of standards predicted declines in systolic BP. Higher levels of self‐critical perfectionism predicted generally sustained levels of diastolic BP, with moderate and low self‐criticism predicting declines in diastolic BP during the repeated stressors. These preliminary results suggest that students with higher levels of perfectionism may be at risk for physiological problems associated with stress reactivity, perhaps especially so in situations in which they experience persistent stress. Although results were qualified by a relatively small sample size, effects were statistically significant and supported the importance of examining the short‐term and long‐term implications of the effects of perfectionism on cardiovascular function and the different implications of elevations in systolic and diastolic BP. Copyright © 2014 John Wiley & Sons, Ltd.     

Egr-1在自体移植静脉中的表达及意义

目的研究自体静脉移植后早期生长反应基因-1(Egr-1)表达的动态变化,探讨其在内膜增生中的作用。方法Wistar大鼠90只,将大鼠右颈总静脉端-端吻合于肾下腹主动脉建立自体静脉移植模型。术后随机分别于1、2、6和24h,3、7、14、28及42d相应时间处死动物取移植静脉,同时取正常静脉作对照。应用原位杂交和RT-PCR方法检测Egr-1mRNA的表达,联合应用Western蛋白印迹和免疫组织化学方法检测Egr-1蛋白表达情况,同时进行组织形态学观察。结果自体静脉移植后,Egr-1mRNA和Egr-1蛋白的表达呈双相变化,即移植后1h,Egr-1mRNA表达迅速升高,阳性率为(35±7)%,6h、24h及3d时下降到较低水平,阳性率分别为(8±2)%、(8±6)%和(8±4)%,7d时又再升高,28d时达高峰,阳性率为(45±6)%,此与其余各时点比较差异均有统计学意义(P<0.01),42d时,Egr-1mRNA的表达再次下降;移植早期(2h)即有Egr-1蛋白的表达,阳性率为(30±5)%,并持续至6h,24h~3d表达下降到较低水平,阳性率分别为(7±3)%和(7±8)%,7d时又再升高,至移植后28d,Egr-1蛋白的表达阳性率达到高峰,为(40±9)%,此与其余各时点比较差异有统计学意义(P<0.01)。移植后7d,免疫组化结果显示,Egr-1蛋白表达主要位于中膜血管平滑肌细胞(VSMCs)和单核细胞/巨噬细胞,移植后期28d,Egr-1蛋白表达主要位于新生内膜和中膜的VSMCs,同时部分内皮细胞也有Egr-1蛋白的表达。结论移植静脉内膜增生与Egr-1的激活及表达关系密切,Egr-1可能成为防治移植静脉内膜增生、狭窄及闭塞的一个新的干预靶点。     

生长激素应用于全身炎症反应综合征时二胺氧化酶及内毒素变化的临床意义

目的 探讨生长激素应用于全身炎症反应综合征时二胺氧化酶(DAO)和内毒索(ET)水平的变化及其与肠粘膜屏障损伤的关系。方法 将146例严重感染性疾病合并全身炎症反应综合征思考分为非手术组和手术组两组，再分别将此两组随机分为治疗组及对照组，于治疗前及治疗后1周监测血浆DA0和ET水平。结果 治疗组血浆DA0及ET水平在治疗后较治疗前下降，且差异有显著性意义(P＜0．05，P＜0．01)。结论 生长激素对严重感染性疾病合并全身炎症反应综合征患者的肠粘膜屏障具有一定的保护作用。