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《中南药学》2019,(7):1018-1023
目的研究经炮制后不同产地桂枝中香豆素、肉桂醇、肉桂酸及桂皮醛的含量变化和抗氧化活性。方法采用高效液相色谱法分别测定广东、广西、云南及福建生、炒、蜜制桂枝中香豆素、肉桂醇、肉桂酸、桂皮醛的含量。色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.1%磷酸溶液(28∶72);流速:1.0 mL·min-1;柱温:30℃;检测波长:290 nm。同时采用紫外分光光度计以DPPH·清除率(IC50值)来评价其抗氧化活性。结果福建炒桂枝中香豆素含量最高,广东生品中肉桂醇含量最高,云南生品中肉桂酸含量最高,福建生品中桂皮醛含量最高;云南蜜制品的抗氧化活性最大。结论本研究建立了同时检测桂枝中4种有效成分的检测方法,该方法简便、可靠、重复性好。不同产地桂枝经不同炮制方法后香豆素、肉桂醇、肉桂酸及桂皮醛的含量及抗氧化活性均有一定的差异。 相似文献
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目的探讨中药用肉桂与食用桂皮的比较方法。方法选择不同药材市场所购的中药用肉桂及食用桂皮,通过观察分析生药来源、性状、显微、薄层色谱,桂皮醛及内酯类检查,分析比较中药用肉桂和食用桂皮的区别。结果中药用肉桂与食用桂皮在来源、性状、显微、薄层色谱,桂皮醛及内酯类检查等方面都存在较为明显的差异。结论通过本文所列的鉴别方法能够有效区分中药用肉桂与食用桂皮,确保中药用肉桂的安全性。 相似文献
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RP-HPLC法测定桂枝中4种活性成分的含量 总被引:3,自引:0,他引:3
目的:建立了同时测定桂枝中桂皮醛、桂皮酸、桂皮醇及香豆素含量的方法,考察了4种活性成分的含量与产地及采收季节的关系。方法:采用反相高效液相色谱法,以Luna C18(4.6mm×250mm,5 μm)为色谱柱,乙腈-0.1%磷酸(28:72)为流动相,检测波长285nm,流速1mL·min-1,柱温30℃。结果:桂皮醛、桂皮酸、桂皮醇及香豆素的线性范围为0.208-3.33μg(r=0.9997),0.100-1.60μg(r=0.9999),0.204-3.26μg(r=0.9999),0.0674-1.08μg(r=0.9999);回收率分别为98.3%(RSD=1.0%),98.0%(RSD=1.4%),99.1%(RSD=1.2%),98.3%(RSD=1.8%)。结论:本法简便、准确、重现性好,适用于桂枝中4种成分的同时测定;生长环境和采收季节对桂枝中4种活性成分的含量有显著影响。 相似文献
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目的建立脾康灵的质量标准。方法采用薄层色谱法对陈皮、肉桂进行定性鉴别;采用高效液相色谱法对肉桂的挂皮醛进行含量测定。色谱柱为Diamonsil C18(250mm×4.6mm,5μm)。流动相为乙腈.水溶液(35:65)。检测波长为290nm。结果薄层色谱能检出陈皮、肉挂;桂皮醛线性关系良好(r=0.9998),平均回收率为98.27%,RSD=1.81%。结论陈皮、肉桂鉴别方法简便、快速,重现性好;高效液相色谱法测定桂皮醛含量准确、快速。 相似文献
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吴垠 《现代食品与药品杂志》2002,12(3):7-7
胃痛定片收载于《卫生部药品标准》中药成方制剂第八册 (WS3 -1 5 80 -93 ) [1] ,该标准无 [鉴别 ]项 ,拟增加肉桂等薄层色谱鉴别 ,提高质量标准。采用无水乙醇超声提取、挥干、溶解、定容、薄层色谱定性 ,结果桂皮醛色谱斑点不明显。以乙醇冷浸[2 ] 所得提取液作为供试品溶液 ,结果所显斑点清晰 ,并与对照品色谱斑点一致。1 仪器及试剂胃痛定片 (广州光华药业股份有限公司 ) ;肉桂对照药材、桂皮醛对照品 (中国药品生物制品检定所 ) ;硅胶G(青岛海洋化工厂 ) ;试剂均为分析纯。2 实验方法与结果供试品溶液的制备∶取胃痛定片 5片 ,研… 相似文献
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<正> 肉桂为樟科植物向桂(Cinnamomum cassia presl)的干燥树皮,肉桂挥发油具有驱风健胃的功能,其主要有效成分为桂皮醛。用β—环糊精制成包结物,可进一步制成散剂、片剂、胶囊剂等固体剂型,使服用方便,桂皮醛等挥发性成分不损失,提高了制剂的质量和疗效。 相似文献
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Jongwon Choi Kyung-Tae Lee Hyeon Ka Won-Tae Jung Hyun-Ju Jung Hee-Juhn Park 《Archives of pharmacal research》2001,24(5):418-423
GC-MS analysis on the essential oil (CC-oil) of Cinnamomum cassia stem bark led to the identification of cinnamaldehyde (CNA, 1), 2-hydroxycinnamaldehyde (2-CNA), coumarin (2), and cinnamyl acetate. The major volatile flavor in CC-oil was found to be 2-CNA. Coumarin was first isolated from this plant by phytochemical isolation and spectroscopic analysis. CNA and CC-oil showed potent cytotoxicity, which was effectively prevented by N-acetyl-L-cysteine (NAC) treatment. Intraperitoneal administration with CNA considerably decreased malondialdehyde (MDA) formation and glutathione S-transferase activity in rats. These results suggest that CC-oil and CNA can regulate the triggering of hepatic drug-metabolizing enzymes by the formation of a glutathione-conjugate. 相似文献
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Quantification of seven phenylpropanoid compounds in Chinese Cinnamomi Cortex and Ramulus by HPLC
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Pengfei Yuan Yajing Ma Dan Su Mingying Shang Feng Xu Guangxue Liu Niloufar Iranmanesh Lanfang Li Tingliang Jiang Shaoqing Cai 《中国药学》2015,24(9):591-599
In the present study, we developed and validated a high-performance liquid chromatography method for the simultaneousdetermination of seven phenylpropanoid compounds (2-hydroxyl cinnamaldehyde, coumarin, cinnamyl alcohol, cinnamic acid, 2-methoxy cinnamic acid, cinnamaldehyde and 2-methoxy cinnamaldehyde) in Cinnamomi Cortex and Cinnamomi Ramulus. The levels of seven phenylpropanoid compounds in Cinnamomi Cortex and Cinnamomi Ramulus were compared using this method. A total of 48 samples (27 Cinnamomi Cortex and 21 Cinnamomi Ramulus) were purchased in China and analyzed. Quantities of seven phenylpropanoid compounds ranged from 17.5 to 61.6 mg/g in Cinnamomi Cortex and ranged from 9.91 to 23.4 mg/g in Cinnamomi Ramulus. The level of 2-methoxy cinnamic acid in the Cinnamomi Cortex samples was below the LOD, whereas it ranged from 0 to 0.119 mg/g in the Cinnamomi Ramulus samples. The (cinnamyl alcohol+cinnamic acid)/cinnamaldehyde ratios (R346) of Cinnamomi Cortex and Cinnamomi Ramulus ranged from 0.0121 to 0.0467 and 0.0598 to 0.182, respectively. This ratio could be used to discriminate Cinnamomi Cortex (<0.05) and Cinnamomi Ramulus (>0.05). The extraction rates (Dn) of seven compounds in boiling water were different, with the lowest dissolution for cinnamaldehyde (<3%) and the highest for cinnamic acid (about 60%). 相似文献
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Tae Woo Kim 《Acta pharmacologica Sinica》2022,(3):712-723
Previous reports suggested that cinnamaldehyde(CA),the bioactive ingredient in Cinnamomum cassia,can suppress tumor growth,migratory,and invasive abilities.Howe... 相似文献
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Byoung-Mog Kwon Seung-Ho Lee Sang Un Choi Sung Hee Park Chong Ock Lee Young-Kwon Cho Nack-Do Sung Song-Hae Bok 《Archives of pharmacal research》1998,21(2):147-152
Cinnamaldehydes and related compounds were synthesized from various cinnamic acids based on the 2′-hydroxycinnamaldehyde isolated from the bark ofCinnamomum cassia Blume. The cytotoxicity to human solid tumor cells such as A549, SK-OV-3, SK-MEL-2, XF498 and HCT15 were measured. Cinnamic acid, cinnamates and cinnamyl alcohols did not show any cytotoxicity against the human tumor cells. Cinnamaldehydes and realted compounds were resistant to A549 cell line up to 15 μg/ml. In contrast, HCT15 and SK-MEL-2 cells were much sensitive to these cinnamaldehyde analogues which showed ED50 values 0.63-8.1 μg/ml. Cytotoxicity of the saturated aldehydes was much weak compared to their unsaturated aldehydes. From these studies, it was found that the key functional group of the cinnamaldehyde-related compounds in the antitumor activity is the propenal group. 相似文献
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目的:建立益经消痛胶囊的质量标准。方法:采用薄层色谱(TLC)法对方中当归、丹参、肉桂、延胡索进行定性鉴别;用高效液相色谱(HPLC)法测定当归中阿魏酸的含量。结果:当归、丹参、肉桂、延胡索的TLC斑点清晰、分离度好;阿魏酸的检测浓度在0.0028~0.0448μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9999);平均回收率为98.46%,RSD=1.53%(n=6)。结论:所建标准可用于益经消痛胶囊的质量控制。 相似文献
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2'-Hydroxycinnamaldehyde, which inhibits farnesyl-protein transferase (FPTase), has been isolated from the stem bark of Cinnamomum cassia Blume. The biologically active agent in the extract has been purified by silica column chromatography and HPLC. The structure of the isolated compound was elucidated on the basis of 500 MHz NMR experiments. 相似文献
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目的:建立肉桂配方颗粒的指纹图谱。方法:采用反相高效液相色谱法。色谱柱为Thermo Hypersil ODS-2(250 mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(28∶72,V/V),检测波长为280 nm,流速为1.0 ml/min,进样量为10μl。采用《中药色谱指纹图谱相似度评价系统软件(2004A版)》对10批样品进行相似度评价。结果:10批肉桂配方颗粒共检出4个共有指纹峰,并指认出其中桂皮醛、肉桂酸、香豆素3个化学成分。10批样品指纹图谱的相似度均>0.900。结论:所建指纹图谱重复性好,可作为肉桂药材及其配方颗粒的质量控制方法。 相似文献