男子青少年赛艇运动员递增负荷运动时肌氧含量变化特点--肌氧评定个体有氧代谢能力的可行性探讨

目的探讨肌氧含量与不同生理指标之间的关系及其评定有氧代谢能力的可行性.方法22名男子青少年赛艇运动员,按运动水平分为A、B两组,在功率自行车上以逐级递增负荷进行蹬车运动至力竭.A组完成6级负荷,B组完成5级负荷.将探测器固定于运动员右侧股外侧肌,测定运动过程中的肌氧含量,并同步测定摄氧量(VO2)、心率(HR)及血乳酸(BLa).结果A组运动员在进入第5级负荷时骨骼肌肌氧水平迅速下降,直到运动结束未达平衡;B组运动员运动开始阶段肌氧水平迅速下降,进入第5级负荷时肌氧水平出现新的平衡.所有受试在负荷较低时肌氧含量呈阶梯状下降,负荷较高时肌氧持续下降,运动停止后肌氧含量迅速超过安静水平.运动过程中,各级负荷末肌氧含量与摄氧量、HR及BLa呈高度负相关(r=-0.943～-0.993).结果提示运动时骨骼肌局部肌氧供需失衡可能与无氧阈有关.赛艇运动员递增负荷训练时肌氧含量下降拐点与乳酸拐点有一定的一致性,可考虑用肌氧含量评定赛艇运动员有氧能力.     

2周低住高练对游泳运动员心肺功能和有氧运动能力的影响

目的:探讨2周低住高练(LoHi)对游泳运动员心肺功能及其有氧运动能力的影响。方法:15名游泳运动员进行为期2周的LoHi训练(模拟海拔2500m),分别在训练前后采用自行车递增负荷力竭运动测试各项气体代谢指标和有氧运动能力。结果:2周LoHi训练后,(1)同等负荷下男运动员VO2/kg低于训练前,第5min50s时相差最大(-21.8%),最大摄氧量平台(VO2/kgmaxPD)持续时间延长了1min;女运动员自第6min后,VO2/kg值与训练前差值逐渐加大,第12min30s时达到最大(-14.5%),VO2/kgmaxPD持续时间缩短了1min30s;(2)男女运动员R值总体上均处于略微下降的趋势,R=1标志点出现时间明显延后。男运动员从R>1到力竭的时间延长,女运动员则缩短;(3)男运动员fb值在中低负荷阶段略高于训练前,女运动员只在10min50s到13min10s阶段低于训练前。不同负荷阶段,男运动员HR值始终低于训练前。训练后女运动员在第5min后HR值略低于训练前。结论:2周LoHi训练提高了男子游泳运动员对负荷的适应能力,表现为一般有氧运动能力和高负荷状态下的无氧耐力水平提高,但女子运动员的运动能力未见明显改善,表现出一定的性别差异。     

中老年人简化太极拳锻炼的气体代谢与能量消耗研究

目的:为不同锻炼年限的中老年人太极拳健身运动处方的制定提供科学依据。方法:运用K4b2气体分析仪,选取中老年男性作为研究对象(多年锻炼者17名,初学者20名),测试简化太极拳练习中(安静期、练习期和恢复期),以及不同架势练习的气体代谢与能量消耗指标。结果:太极拳初学者与多年锻炼者恢复期心率(HR)、氧脉搏(VO2/HR)有显著性差异(P<0.05),摄氧量(VO2)、通气量(VE)有非常显著性差异(P<0.01)。太极拳练习期,高架势状态下初学者与多年锻炼者相比,通气量、心率、氧脉搏、摄氧量、相对摄氧量(VO2/kg)和能耗(EE)均无显著性差异(P>0.05);多年锻炼者自身高低架动作比较,呼吸频率(RF)、氧脉搏无显著性差异(P>0.05),通气量、心率、能耗有显著性差异(P<0.05),摄氧量、相对摄氧量具有非常显著性差异(P<0.01)。结论:多年太极拳锻炼者运动后机能恢复较快,其呼吸深度较大,能满足较低架势动作的摄氧需要;多年锻炼者简化太极拳练习中运动强度的差异主要源于练习架势的高低。     

耐力训练对青少年运动员最大摄氧量和动脉血酮体比的影响

目的:观察耐力训练对青少年运动员有氧能力和动脉血酮体比(AKBR)的影响。方法:18名青少年游泳运动员4周耐力训练前后,测定其递增负荷运动实验中VO2max、血乳酸和心率变化,以及恒定负荷运动实验后酮体含量和动脉血酮体比。结果:4周耐力训练后,受试者VO2max、最大运动时的负荷、心率、血乳酸水平较训练前显著提高,个体乳酸阈呈升高趋势;递增负荷运动中的摄氧量、心率和血乳酸动力学曲线右移;动脉血酮体比显著提高。结果表明,伴随青少年游泳运动员有氧运动能力的改善,在等量负荷运动条件下其肝线粒体氧化还原状态和能荷增强。     

男子散打运动员气体代谢和血液流变性特点分析

目的:探讨男子散打运动员气体代谢和血液流变性特点。方法:15名男子散打运动员(实验组)和15名普通男子大学生(对照组)进行递增负荷运动,分别于安静状态、运动过程中及运动后即刻测试气体代谢及血液流变学指标。结果:安静时,实验组ηH、ηL、ESR、Arbc、TK、ESRK和EPT显著低于对照组;运动后即刻,实验组ηb、TK、ESRK、EPT较安静时显著增加,但增加幅度显著低于对照组。最大摄氧量时,实验组VO2max、VCO2、VO2/HR、TV和VE显著高于对照组,VEO2显著低于对照组;通气阈时,实验组VT、VO2、VCO2、VO2/HR、VE、TV、RR和METS显著高于对照组,VEO2显著低于对照组。结论:安静状态下,散打运动员全血粘度和红细胞聚集性低于普通大学生,红细胞变形性高于普通大学生;运动后,散打运动员血液流变性优于普通大学生。散打运动员具有通气效率高、氧气利用率高、摄氧能力强的气体代谢特点。     

上肢有氧训练对因伤停训运动员康复早期心肺功能的影响

目的:采用运动心肺试验(CPET)和上肢功率车有氧训练方案对膝关节交叉韧带重建术后停训运动员的有氧耐力和心肺功能进行评价和干预,为改善该类患者早期康复训练方案提供依据。方法:25名行膝关节交叉韧带重建术运动员分为3组:(1)对照组(7人),术后第一天起按常规康复训练处方训练(包括床旁训练、肌力训练、关节活动度训练等,每次30 min至1 h,每天2次,每周5天,共4周);(2)持续训练组(8人),加入持续上肢功率车有氧训练,强度为60%~70%VO2peak,30 min/组,1组/天,3~5天/周,共4周;(3)间歇训练组(10人),加入间歇上肢功率车有氧训练,4 min 50%~60%VO2peak+1 min 90%~100%VO2peak,30 min/组,1组/天,3~5天/周,共4周。受试者均在术前1周内和有氧训练4周后1周内完成上肢功率车CPET的监测,采集其有氧耐力和心肺功能指标。结果:4周后,对照组峰值摄氧量(VO2peak)、相对峰值摄氧量(VO2peak/kg)、代谢当量(MET)、氧脉搏(O2Plus)、每搏输出量(SV)较4周前显著性降低(P<0.05),且初始VO2peak与其下降幅度高度负相关;持续训练组O2Plus较4周前显著性降低(P<0.05),间歇训练组VO2peak、VO2peak/kg、MET、O2Plus、SV较4周前均显著性增加(P<0.05,P<0.01)。结论 :运动员术后4周常规康复训练心肺功能显著降低,且VO2peak的下降程度与初始VO2peak成高度负相关;加入4周上肢功率车有氧训练组心肺耐力显著改善,且间歇训练组较持续训练组效果更佳。     

Verification phase作为判定最大摄氧量新方法的研究进展

最大摄氧量(VO2max)是评价心肺功能和有氧能力的金指标,递增负荷运动测试(GXT)是最常用的测定VO2max的方法,摄氧量平台是确定VO2max的金标准,但其确定标准和发生率均报道不一,而判定VO2max的次级指标(RERmax、HRmax、BlAmax、RPE)缺乏明确标准、敏感性和可靠性。近些年来,verification phase作为判定VO2max的新方法,在国外已进行了大量研究,具有实用性和敏感性,目前国内关于verification phase的相关研究报道尚少,本文就verification phase的开展要素如运动强度、持续时间、运动方案、恢复时间、采样时间间隔及其判定标准等研究进展进行综述。     

不同负荷形式的生理生化指标与武警战士的有氧代谢能力评价研究

 目的研究不同负荷形式的生理生化指标变化与武警战士的有氧代谢能力的关系,为有氧代谢能力的评定提供理论和实践依据.方法通过亚极量踏阶运动间接法,测定VO2max(最大摄氧量),选取通过有针对性的有氧耐力训练后,VO2max较高的1组作为实验组,较低的1组作为对照组,对两组战士分别进行极量负荷和定量负荷实验,测定负荷后和某负荷阶段的有关生理生化、生理心理指标.结果定量负荷后,实验组的血乳酸(Bla)和心率(HR)值均低于对照组(P＜0.01);负荷后的恢复期,实验组的指标变化情况优于对照组(P＜0.05或P＜0.01),在运动过程中,随着负荷量的增加,实验组的主体感觉(RPE)值低于对照组.极量负荷后,两组间的HR值无显著性差异,实验组的Bla高于对照组.结论不同负荷形式的生理、生化指标的变化能够反映武警战士不同的有氧代谢能力,在选择有氧耐力训练安排中有应用价值.     

我国优秀男子现代五项运动员有氧能力特征

目的:了解我国男子优秀现代五项运动员的有氧能力特征。方法:测试对象为中国国家男子现代五项队运动员10名,测试最大摄氧量(VO2max),判定运动员的无氧阈(AT)。测试仪器采用德国产Jaeger Oxycon Pro心肺功能仪以及LE6000跑台进行测试,最大摄氧量(VO2max)测试程序是以10km/h、0.5%坡度起始,每分钟递增0.8km/h,速度达到18.0km/h时每分钟递增1%坡度。运动至力竭。结果:我国现代五项运动员的VO2max测值为4.86±0.43L/min和62.9±4.58ml·kg-1·min-1,VEmax为153.8±15.25L/min,HRmax为195.8±12.69b/min,O2/HR为24.67±2.83ml。VT-VO2为3.72±0.43L/min和48.12±3.35ml·kg-1·min-1,VT%VO2max为76.50±4.03,VT-HR为176.5±10.16b/min,VT-V为15.44±0.21km/h。结果表明:我国男子现代五项运动员的有氧能力水平低于其他相关的耐力性项目运动员,应加强有氧能力的训练,提高有氧能力。我国男子现代五项重点运动员有氧能力水平差异较大,应根据个性特征进行有针对性的个性化训练安排。     

瘦素受体基因多态性与有氧耐力及其训练效果的关联性研究

目的:探讨瘦素受体(OB-R)基因第6外显子Gln223Arg多态性与有氧耐力及有氧耐力训练效果的关联性。方法:对新征入伍的102名中国北方汉族士兵进行为期18周、每周3次、每次5000米、强度为95~105%通气阈(VT)的耐力训练。采用跑台逐级递增负荷运动实验测定受试者训练前后最大摄氧量(VO2max),并在12km/h跑速时测定跑节省化(RE)时的心率(HR)、通气量(VE)和摄氧量(VO2);利用限制性片断长度多态(PCR-RFLP)法进行OB-R基因Gln223Arg多态性解析。结果:(1)OB-R基因Gln223Arg多态性在中国北方汉族人群中的分布频率分别为AA:3%、AG:25%、GG:72%,符合Hardy-Weinberg遗传平衡定律;(2)按基因型分组后,A等位基因携带者(AA+AG)训练前的最大摄氧量相对值明显高于未携带者(GG),其他指标训练前后在两组之间无显著性差异。结论:OB-R基因第6外显子的Gln223Arg多态性与有氧能力可能存在关联,而与有氧耐力训练效果不关联。     

Muscle deoxygenation as related to work rate

PURPOSE: The kinetics of the decrease in venous O(2) content in response to constant work rate exercise below the lactic acidosis threshold (LAT) is very rapid, reaching a constant value by approximately 1 min. However, for work rates above the LAT, a slow further decrease in venous O(2) content takes place that is attributable to the Bohr effect rather than further decrease in end capillary PO. We hypothesized that similar differences, with respect to the LAT, will be observed in muscle deoxygenation kinetics when studied with near-infrared spectroscopy (NIRS). METHODS: Twelve normal subjects performed three constant work rate tests from unloaded cycling at 60% of LAT, 80% LAT, each with four repetitions, and above LAT (LAT + 35% between LAT and VO(2max) three times, on a cycle ergometer for 6 min. We measured tissue deoxygenation with NIRS, with the probe over the vastus lateralis muscle, time-averaging the repetitions. Gas exchange and heart rate (HR) were measured breath-by-breath and beat-by-beat. RESULTS: Tissue deoxygenation kinetics were significantly faster than VO(2) and HR at 60%- and 80%-LAT work rates. By 1 min of exercise, deoxygenation was constant for the work rate below the LAT. At 30 s, tissue deoxygenation was 70-95% complete, whereas VO(2) and HR were only 30-60% complete. For the work rate above the LAT, a steady state for muscle deoxygenation was not reached during the 6 min of exercise. After 1 min of above-LAT exercise, either one of two patterns of slow change in tissue oxygenation developed, deoxygenation or reoxygenation. It is postulated that these different responses might be due to effects of the exercise lactic acidosis. H accompanying lactate increase might cause further deoxygenation due to the Bohr effect, and acidosis-induced vasodilatation might cause reoxygenation after the initial deoxygenation. CONCLUSION: 1) The kinetics of tissue deoxygenation are significantly more rapid than VO(2) and HR kinetics at all work rates studied, and 2) steady-state in tissue deoxygenation is seen by 1 min of constant work rate exercise below the LAT, but this is much delayed for work rates above the LAT.     

Effects of different doses of caffeine on exercise responses in young children

INTRODUCTION/PURPOSE: This study investigated the effects of three different doses of caffeine on physiological responses to exercise in young children. METHODS: Forty healthy children (20 boys and 20 girls) volunteered for a random, double-blind, counterbalanced study where they received either placebo (PL), 1 mg.kg(-1) (CAF-1), 3 mg.kg(-1) (CAF-3), or 5 mg.kg(-1) (CAF-5) caffeine, 60 min prior to preexercise measures, followed by cycle ergometer exercise at 25 W and then 60% VO2peak. During this time, heart rate (HR), oxygen consumption (VO2), and respiratory exchange ratio (RER) were measured continuously, while blood pressure (BP) was measured every 2 min. RESULTS: There were no significant gender x treatment interactions, and so the boys' and girls' data were combined. At preexercise, CAF-5 SBP and DBP were significantly (P < 0.05) higher than PL. Both CAF-1 and CAF-3 DBP were also significantly (P < 0.05) higher versus PL at preexercise. There were no treatment effects for exercise BP. At rest and during both exercise intensities, HR was significantly (P < 0.05) lower in CAF-3 (approximately 5 bpm) and CAF-5 (approximately 6 bpm) versus PL. There were no significant effects of CAF on metabolism (VO2 or RER) except for a lower RER in CAF-1 versus CAF-5 at 60% VO2peak. CONCLUSION: Low, mild, and moderate (1, 3, and 5 mg.kg(-1)) doses of caffeine have no effect on substrate use as reflected by RER. Further, caffeine intake resulted in an increase in BP and decrease in HR at preexercise and a slight decrease in HR with CAF-3 and CAF-5 versus PL during exercise.     

Adaptation to a standardized training program and changes in fitness in a large, heterogeneous population: the HERITAGE Family Study

PURPOSE: This paper describes the variations in response to a standardized, computer-controlled training program. METHODS: Steady-state heart rate (HR) and oxygen intake (VO2) of 614 healthy, sedentary men and women aged 16-65 yr were measured during three cycle ergometer exercise tests. The HR associated with 55, 65, 70, and 75% of each subject's pretraining VO2max was used to prescribe exercise intensity. Subjects exercised three times a week, beginning at a HR associated with 55% VO2max for 30 min. Duration and intensity was gradually increased over 20 wk of training. The duration and HR of each training session were controlled by a computer. RESULTS: Using the linear relationship between HR, VO2 and power output (PO), PO were predicted for each of 60 training sessions at the respective programmed HR. The average ratio of the actual training HR to programmed HR was 0.99. It was hypothesized that participants whose actual training PO exceeded their predicted PO would improve VO2max more than those whose actual PO was less than their predicted PO. Using the ratio of actual/predicted PO determined after the training was over, participants were arbitrarily assigned to three groups: 128 participants had low (LO) ratios (0.65-0.84), 408 had average (AV) ratios (0.85-1.14), and 78 had high (HI) ratios (1.15-1.34). Secondary analysis showed that the training program significantly increased mean VO2max of all three groups. Those who had a smaller increase in training PO (LO) had significantly less increase in VO2max than those with larger increases in PO (HI). CONCLUSION: People who exercise at a HR associated with the same %VO2max can vary substantially in their training PO, in their rate of increase in PO over a 20-wk training program, and in improvement of their VO2max.     

Simulated hypergravity running increases skeletal and cardiovascular loads

PURPOSE: Eight recreational and eight competitive athletes were studied to determine the cardiovascular and musculoskeletal effects of running in hypergravity conditions simulated by the use of lower body negative pressure (LBNP). Subjects are sealed in a LBNP chamber with the use of a flexible, neoprene waist seal and the chamber pressure is reduced to provide an increased vertical force. METHODS: Subjects ran on a treadmill at 1.0, 1.1, and 1.2 times body weight (BW) with increased loads provided by LBNP. Heart rate (HR), oxygen consumption (VO(2)), vertical ground reaction force (GRF), dynamic knee range of motion (ROM), and the electrical activities (EMG) of the tibialis anterior, medial gastrocnemius, vastus medialis obliquous, and biceps femoris muscles were measured. RESULTS: LBNP produced a significant increase in HR at the 1.1-BW and 1.2-BW levels in both recreational and competitive athletes when compared with the 1.0 BW condition. Both VO(2) and GRF were increased significantly at 1.2 BW. No significant changes were observed in knee ROM or peak EMG amplitude with LBNP in either recreational or competitive athletes. CONCLUSION: Increased HR and VO(2) indicate an increased cardiovascular load whereas increased GRF indicates an increased skeletal load. The lack of change in muscle activation and knee ROM point to a preservation in gait mechanics. Increased cardiovascular and skeletal loads with preservation of gait mechanics suggest that exercise within LBNP may be an effective training modality to improve athletic performance.     

富氧室在海拔3700m对人体体力活动时氧耗量的影响

目的观察在海拔3700m建立富氧室对人体体力活动时氧耗量的影响。方法:10名受试者在进入富氧室前后分别观察氧耗量(VO2)及氧脉搏(VO2/HR)。结果:心率在100b-170b/min时,富氧室随HR增加而呈线性增加[分别为Y=(0.0165+0.0073)x,r=0.9877,P<0.001;Y=(-0.1629+0.0087)x,r=0.9902,P<0.001]。结论:富氧室在高原能提高心脏作功效率和改善肺功能。     

Limiting factors for maximum oxygen uptake and determinants of endurance performance

In the exercising human, maximal oxygen uptake (VO2max) is limited by the ability of the cardiorespiratory system to deliver oxygen to the exercising muscles. This is shown by three major lines of evidence: 1) when oxygen delivery is altered (by blood doping, hypoxia, or beta-blockade), VO2max changes accordingly; 2) the increase in VO2max with training results primarily from an increase in maximal cardiac output (not an increase in the a-v O2 difference); and 3) when a small muscle mass is overperfused during exercise, it has an extremely high capacity for consuming oxygen. Thus, O2 delivery, not skeletal muscle O2 extraction, is viewed as the primary limiting factor for VO2max in exercising humans. Metabolic adaptations in skeletal muscle are, however, critical for improving submaximal endurance performance. Endurance training causes an increase in mitochondrial enzyme activities, which improves performance by enhancing fat oxidation and decreasing lactic acid accumulation at a given VO2. VO2max is an important variable that sets the upper limit for endurance performance (an athlete cannot operate above 100% VO2max, for extended periods). Running economy and fractional utilization of VO2max also affect endurance performance. The speed at lactate threshold (LT) integrates all three of these variables and is the best physiological predictor of distance running performance.     

Effects of short-term training on cardiac function during prolonged exercise

To determine the relationship between the training-induced increases in plasma volume (PV) and alterations in cardiac performance during prolonged submaximal cycle exercise, seven male subjects were studied prior to and following a short-term (3 d) training period (2 h.d-1 at 65% VO2max). Mean (range) VO2max was 3.42 l.min-1 (2.96-3.87). Training resulted in a 20% increase (P less than 0.05) in plasma volume (PV) and a 12% increase (P less than 0.05) in total blood volume (TBV). In contrast, training had no effect (P greater than 0.05) in altering exercise VO2, VCO2, VE BTPS, or RER. Cardiac output (Q) was higher (P less than 0.05) posttraining at all exercise sampling times (30, 60, 90, and 120 min). The elevations in Q were accompanied by an average decrease (P less than 0.05) in stroke volume (SV) of 22 ml. Arteriovenous O2 (a-v O2) difference was depressed (P less than 0.05) during exercise following the training. Although elevations (P less than 0.05) in core temperature (degrees C) occurred during the exercise, the training-induced PV increases did not affect thermoregulatory behavior. These results indicate that an early adaptive response to exercise training is an elevation in Q, an increase in SV, and a reduction in HR. These effects persist during prolonged exercise in spite of the progressive increase in body heat content. It is proposed that the increase in Q serves primarily to increase muscle blood flow and maintain arterial O2 delivery, while the altered cardiodynamic behavior serves to increase cardiac reserve, providing a greater tolerance to prolonged heavy exercise.     

The effect of detraining and reduced training on the physiological adaptations to aerobic exercise training

In previously sedentary individuals, regularly performed aerobic exercise results in significant improvements in exercise capacity. The development of peak exercise performance, as typified by competitive endurance athletes, is dependent upon several months to years of aerobic training. The physiological adaptations associated with these improvements in both maximal exercise performance, as reflected by increases in maximal oxygen uptake (VO2max), and submaximal exercise endurance include increases in both cardiovascular function and skeletal muscle oxidative capacity. Despite prolonged periods of aerobic training, reductions in maximal and submaximal exercise performance occur within weeks after the cessation of training. These losses in exercise performance coincide with declines in cardiovascular function and muscle metabolic potential. Significant reductions in VO2max have been reported to occur within 2 to 4 weeks of detraining. This initial rapid decline in VO2max is likely related to a corresponding fall in maximal cardiac output which, in turn, appears to be mediated by a reduced stroke volume with little or no change in maximal heart rate. A loss in blood volume appears to, at least partially, account for the decline in stroke volume and VO2max during the initial weeks of detraining, although changes in cardiac hypertrophy, total haemoglobin content, skeletal muscle capillarisation and temperature regulation have been suggested as possible mediating factors. When detraining continues beyond 2 to 4 weeks, further declines in VO2max appear to be a function of corresponding reductions in maximal arterial-venous (mixed) oxygen difference. Whether reductions in oxygen delivery to and/or extraction by working muscle regulates this progressive decline is not readily apparent. Changes in maximal oxygen delivery may result from decreases in total haemoglobin content and/or maximal muscle blood flow and vascular conductance. The declines in skeletal muscle oxidative enzyme activity observed with detraining are not causally linked to changes in VO2max but appear to be functionally related to the accelerated carbohydrate oxidation and lactate production observed during exercise at a given intensity. Alternatively, reductions in submaximal exercise performance may be related to changes in the mean transit time of blood flow through the active muscle and/or the thermoregulatory response (i.e. degree of thermal strain) to exercise. In contrast to the responses observed with detraining, currently available research indicates that the adaptations to aerobic training may be retained for at least several months when training is maintained at a reduced level. Reductions of one- to two-thirds in training frequency and/or duration do not significantly alter VO2max or submaximal endurance time provided the intensity of each exercise session is maintained.(ABSTRACT TRUNCATED AT 400 WORDS)     

The effect of high‐intensity training on mitochondrial fat oxidation in skeletal muscle and subcutaneous adipose tissue

High‐intensity interval training (HIT) is known to increase mitochondrial content in a similar way as endurance training [60–90% of maximal oxygen uptake (VO_2peak)]. Whether HIT increases the mitochondria's ability to oxidize lipids is currently debated. We investigated the effect of HIT on mitochondrial fat oxidation in skeletal muscle and adipose tissue. Mitochondrial oxidative phosphorylation (OXPHOS) capacity, mitochondrial substrate sensitivity (K_m^app), and mitochondrial content were measured in skeletal muscle and adipose tissue in healthy overweight subjects before and after 6 weeks of HIT (three times per week at 298 ± 21 W). HIT significantly increased VO_2peak from 2.9 ± 0.2 to 3.1 ± 0.2 L/min. No differences were seen in maximal fat oxidation in either skeletal muscle or adipose tissue. K_m^app for octanoyl carnitine or palmitoyl carnitine were similar after training in skeletal muscle and adipose tissue. Maximal OXPHOS capacity with complex I‐ and II‐linked substrates was increased after training in skeletal muscle but not in adipose tissue. In conclusion, 6 weeks of HIT increased VO_2peak. Mitochondrial content and mitochondrial OXPHOS capacity were increased in skeletal muscle, but not in adipose tissue. Furthermore, mitochondrial fat oxidation was not improved in either skeletal muscle or adipose tissue.