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雷公藤乙酸乙酯提取物的含量测定方法 总被引:2,自引:0,他引:2
本文讨论了雷公藤的提取方法及乙酸乙酯提取物总二萜含量测定方法。方法雷公藤根茎先用乙醇提取,然后用乙酸乙酯洗提,最后挥干溶剂得干品。结果雷公藤根乙酸乙酯提取物以干品计其总二萜含量为0.51%~0.71%。结论此方法灵敏度高、操作方便,结果准确。 相似文献
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中草药多糖含量测定方法概述 总被引:25,自引:3,他引:25
多糖是由10个以上的单糖基通过苷键连接而成的大分子化合物,传统的观念认为多糖生理活性不强,在中草药提取制备过程中常将多糖作为杂质除去.自20世纪70年代以来陆续发现多糖及多糖复合物参与了细胞生命的代谢和调节,中草药多糖在增强机体免疫功能及抗肿瘤、抗肝炎、抗溃疡、调血脂、降血糖、抗衰老方面有作用[1~3].这些多糖无细胞毒性,其含量可通过化学手段加以控制.因此,在对中草药进行研究过程中,在重视其他化学成分的同时亦应关注多糖的含量.本文对近年来中草药多糖的含量测定方法作一概述. 相似文献
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板蓝根多糖的提取及含量测定 总被引:11,自引:0,他引:11
目的:从板蓝根中的提取多糖,并测定其含量。方法:用水提醇沉法提取板蓝根多糖,用酚-硫酸比色法测定多糖含量。结果:测得板蓝根中多糖含量0.8099%,平均回收率为98.74%,RSD=1.86%(n=5)。结论:板蓝根多糖含量不是很高,提取方法有待进一步改进。 相似文献
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Triterpenes from Tripterygium wilfordii Hook 总被引:1,自引:0,他引:1
Yang JH Luo SD Wang YS Zhao JF Zhang HB Li L 《Journal of Asian natural products research》2006,8(5):425-429
Two new friedelane-type triterpenes, tripterfrielanons A (1) and B (2), along with six known triterpenoids, friedelin (3), canophyllal (4), canophyllalic acid (5), 3-oxo-29-hydroxyfriedelane (6), wilforlide A (7), wilforlide B (8), have been isolated from the EtOH extract of the roots of Tripterygium wilfordii Hook.f. Compounds 4, 5, 6 were isolated for the first time from this plant. The new triterpenes 1 and 2 exhibited mild cytotoxic activity against human Hela cell lines in vitro. The assay showed the IC50 of 1 and 2 were 8.5 and 25 μg/mL, respectively. 相似文献
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雷公藤多苷对女性生殖内分泌系统的影响 总被引:4,自引:0,他引:4
雷公藤多苷(GTW)是一种临床应用广泛的免疫抑制剂,对女性生殖系统功能有明显抑制作用。本文主要从GTW的生殖抑制作用研究概况,及研究中存在的分歧等方面进行综述,以期为GTW的进一步药理研究及临床应用提供参考。 相似文献
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Guang-Zhong Yang Ming-Lei Xi Yuan-Chao Li 《Journal of Asian natural products research》2013,15(2):83-88
Abstract Two novel phenolic triterpenes were isolated from Tripterygium wilfordii Hook. f., their structures were identified to be 2,3-dihydroxy-1,3,5(10),8-tetra-ene-6α-(2′-hydroxyethyl)-24-nor-D:A-friedooleanane-29-oic acid 1, named triptotin F, and 2,3-dihydroxy-1,3,5(10),8-tetraene-6β-(2′-hydroxyethyl)-24-nor-D:A-friedooleanane-29-oic acid 2, named triptotin G on the basis of spectroscopic studies. 相似文献
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雷公藤多甙抑制小鼠腹腔巨噬细胞产生一氧化氮 总被引:16,自引:0,他引:16
雷公藤多甙(polyglycosideofTripterygiumwilfordiHook.f.,TWP)临床广泛用于治疗类风湿性关节炎等多种自身免疫性疾病,既可抑制细胞免疫又可抑制体液免疫[1].但其在免疫抑制过程中对一氧化氮合酶(NOS)的影响却... 相似文献
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正相HPLC法测定雷公藤药材及其制剂中雷公藤酯甲的含量 总被引:5,自引:0,他引:5
目的:建立正相高效液相色谱法对雷公藤药材和雷公藤多苷片中雷公藤酯甲的含量测定方法。方法:采用Alltima Silica色谱柱。流动相:正己烷-异丙醇(95:5)。采用蒸发光散射检测器,漂移管温度:70℃,气流速:1.8L/min。结果:雷公藤酯甲在0.64~3.2μg范围内线性关系良好(r=0.9998),平均回收率为97.98%,RSD=4.31%(n=5)。结论:本方法简便,灵敏,准确,重现性好,可用于雷公藤药材及其制剂的含量测定和质量控制。 相似文献
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目的:利用现代计算机技术与统计学分析相结合的方法探析雷公藤在治疗类风湿性关节炎(rheumatoid arthritis,RA)时的用药规律。方法:全面收集2006年6月至2016年6月国内外期刊公开发表的、含雷公藤且对RA有一定临床疗效的中药处方,建立处方数据库,采用IBM SPSS Modeler 14.1进行频数分析和关联度分析,比较雷公藤在治疗RA时,内服方剂和外用方剂中的配伍规律,以及内服与外用的用药差异。结果:雷公藤治疗RA时,收集的药方共214个,包括外用方55个,内服方159个。口服给药配伍频次较高的是甘草、白芍、青风藤、地黄、鸡血藤、黄芪、当归、独活、薏苡仁、桂枝等,补虚药、祛风湿药、解表药居多;外用方中药物频次较高的包括乳香、没药、川芎、细辛、川乌、白芍、红花、草乌、威灵仙等,祛风湿药和活血化瘀药所占比例较大。经关联度和网络图分析得雷公藤在口服和外用两种给药方式时配伍用药存在一定的差异。结论:文本挖掘能比较客观地总结出雷公藤在治疗RA时的用药规律,为雷公藤的配伍减毒的机制研究和临床应用提供参考。 相似文献
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雷公藤醋酸乙酯提取物对大鼠垂体的影响 总被引:2,自引:0,他引:2
雷公藤是一种有效的抗风湿中草药 ,临床和实验研究表明其具有多种药理学功能 .实验将 30只雄性 Sprague- Dawley大鼠完全随机分成雷公藤醋酸乙酯提取物 (TWEE)组 ,泼尼松组和对照组 .分别 ig给药 TWEE2 5mg·kg-1,每日 2次 ;泼尼松2 mg· kg-1,每日 2次和 0 .5%羧甲基纤维素钠 30d,所有大鼠于 d31断头处死 ,分离垂体和收集血液 .对垂体进行 HE和免疫细胞化学染色 ;应用放射免疫分析法检测血浆促肾上腺皮质激素 (ACTH)含量 .结果表明 TWEE能促进大鼠垂体远侧部ACTH细胞合成和分泌 ACTH.为临床综合评价雷公藤对下丘脑 -垂体 -肾上腺轴的影响提供了实验数据和理论依据 . 相似文献
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Yu He Senlin Shi Ruping Zhang Wei Shen Jue Tu Zhishan Ding 《Drug and chemical toxicology》2015,38(2):145-151
Tripterygium wilfordii Hook. f. (TW) is a traditional herbal medicine which has been widely used for the treatment of rheumatoid arthritis and other autoimmune diseases. However, adverse reactions of TW such as hepatotoxicity and nephrotoxicity have been frequently reported in clinic. With the aim to evaluate the potency and toxicity of TW, we collected eleven batches of TW from different localities across Chinese mainland, and investigated the inhibition of their methanol extracts on the proliferation of mouse spleen lymphocytes, normal human hepatocyte (L-02) cells and African green monkey kidney (COS-7) cells. TW extracts with three different concentrations were designed as the experimental groups. Our present findings provided consistent evidence that TW had significant concentration-dependent inhibitory action on lymphocytes, L-02 and COS-7 cells. At the concentrations of 0.75 and 1.5?mg/mL, most TW groups showed statistically significant inhibition of lymphocyte proliferation when compared with the control group (p?0.01), and the inhibition of TW extract on lymphocytes was almost equal to 1.0?mg/mL aspirin (p?>?0.05). In most test groups, significant toxicities were shown on L-02 cells at 0.6 and 3.0?mg/mL (p?0.01), and on COS-7 cells at 3.0?mg/mL (p?0.01). At 3.0?mg/mL, almost all TW groups exerted obvious toxicities toward L-02 and COS-7 cells which were equal to or even higher than 1.0?mg/mL aspirin. In view of these results, further studies are needed to elucidate the relations among the effective component, curative effect and toxicity of TW to ensure its effectiveness and safety for human consumption. 相似文献