含A蛋白葡萄球菌对小鼠肝癌恶性淋巴瘤及白血病免疫预防效果的进一步研究

615系小鼠及昆明种小鼠经含A蛋白葡萄球菌SpA菌体经腹腔免疫后两周,分别用小鼠腹水肝癌(H_(22)),恶性淋巴瘤(ARS)及白血病(L7712)细胞1×10~5腹腔攻击,观察2个月,实验结果表明:SpA菌体免疫对H_(22)肝癌细胞攻击的保护率为30％,抑制率为143.6％;对ARS恶性淋巴瘤细胞攻击的保护率为15％,抑制率达207.8％;对L7712白血病细胞攻击的抑制率为216.0％。SpA菌体诱导免疫保护效应强度不低于厌氧棒状杆菌(CP)组免疫。这些结果说明,SpA菌体腹腔免疫能诱导非特异的抗肿瘤免疫效应。     

含A蛋白葡萄球菌对肉瘤S180和小鼠恶性淋巴瘤ARS免疫治疗效果的初步观察

小鼠腹腔接种1×10~5个小鼠肉瘤S180或恶性淋巴瘤(ARS)瘤细胞后,腹腔注射SpA菌体,可获得明显的免疫治疗效果。注射SpA菌体时间越接近瘤细胞接种日期,免疫治疗效果越好,对S180和ARS的保护率可分别达44.5—55.4％和62.5—80％,相反,给SpA菌体的时间越晚,治疗效果越差,这表明SpA菌体的免疫治疗效果与瘤细胞增殖数量有关。根据实验结果,对SpA菌体作为免疫调整剂用于肿瘤治疗的可能性进行了讨论。     

转输小鼠脾细胞对小鼠白血病(L615)的防治作用

本文报告了转输正常小鼠脾细胞对615系小鼠白血病(L615)攻击的保护效应。如果在攻击前7天转输,五种具有不同遗传学背景的正常小鼠脾细胞都有明显的保护效应;但若在攻击前28天转输,其保护效应则因供者品系而异;随着E/T(效应细胞数/肿瘤细胞数)比值降低其保护效应也有降低的趋势。如果将相同数量的正常小鼠脾细胞装入扩散盒,然后将其植入小鼠腹腔,则不能诱导保护效应。转输正常肾细胞或注射正常脾细胞匀浆对L615白血病细胞攻击也无任何保护效应。实验组大多数存活小鼠都能耐受剂量递增(10~4—10~6)的L615白血病细胞的反复攻击。在继承性化学免疫治疗实验中这些存活小鼠的免疫脾细胞能延长晚期L615病鼠的中位存活时间,甚至能使50%(5/10)的小鼠获得治愈。     

葡萄球菌A蛋白抗癌机理研究 Ⅰ、葡萄球菌A蛋白对小鼠巨噬细胞超微结构和细胞毒作用的影响

通过电镜和~(125)Ⅰ—udR标记肿瘤靶细胞DNA释放试验,对腹腔或皮下注射SpA或SpA菌体后小鼠腹腔巨噬细胞的超微结构、及其对靶细胞的细胞毒效应进行了动态观察。结果首次证明,小鼠腹腔注射SpA菌体后第5、14d,其腹腔巨噬细胞体积明显增大,可达对照组的2—3倍;皱褶明显增多,有众多的伪足和指状突起,外形极不规则,形似海星;胞质内细胞器明显增多,尤以粗面内质网和溶酶体为显著,胞质内充满吞噬体。实验进一步证明,小鼠腹腔注射SpA或SpA菌体后第5d,其腹腔巨噬细胞杀伤效应(细胞毒分别为42.0±7.8％和46.3±11.7％)比对照组(细胞毒为32.0±13.8％)明显增强,至第7d,细胞毒效应达峰值水平(分别为50.4±17.3％和56.9±13.4％)。根据实验结果,对SpA增强巨噬细胞杀伤效应的可能机理进行了讨论。     

各种瘤苗对L615免疫保护效应的进一步实验研究

本文采用莪术及其有效单体β-榄香烯处理的L615细胞进行免疫,观察其免疫保护效应,并与用多种理化及生物学方法制备的L615瘤苗的免疫保护效应相比较。结果表明,用莪术油中提取的有效单体成分β-榄香烯制备的瘤苗及莪术瘤苗均有明显的免疫保护效应,两者的效果相近,均优于~(60)Co照射瘤苗。本实验中所应用的以其它化学和生物学方法包括丝裂毒素、戊二醛处理的及CP和瘤细胞混合瘤苗;都可诱发不同程度抗同系L615攻击的免疫力。免疫保护效应可通过活瘤细胞的反复攻击而不断增强,最终可耐受高达5×10~8个活L615细胞的攻击。     

L759与L615瘤细胞抗原性的初步比较观察——瘤苗主动免疫及交叉攻击试验

L759是在615近交系小鼠上新建立的一株可移植性肉瘤白血病。由于 L759的原带代瘤鼠是一只莪术 L615瘤苗+BCG 主动免疫后经10~5个活 L615细胞攻击而存活的小鼠,它很可能并非自发瘤,而是在免疫机体中由 L615细胞遗传性发生变异而来。因而弄清它与 L615的关系,具有一定的理论意义。我们通过瘤苗主动免疫及交叉攻击试验,对 L759与 L615瘤细胞的抗原性进行了初步的比较,结果并未发现两者之间有     

10例自体骨髓移植造血重建动力学观察

本文报告10例自体骨髓移植(ABMT)后患者造血重建的结果。白细胞在平均6.6天降为0,其恢复至0.5×10~9/L、1.0x10~9/L、4×10~9/L时间分别为平均移植后18.5天、23.4天、51.3天;中性粒细胞恢复至0.1×10~9/L、0.5×10~9/L时间为21.6天和33.5天;血小板在移植后4～14天降最低值(2～16)×10~9/L,其恢复至20×10~9/L、50×10~9/L时间为平均16.5天和39天。通过     

复方蟾皮胶囊的辐射增效作用及其对离体癌细胞的影响

作者在小鼠腹股沟皮下接种H_(22)瘤细胞4 d,局部~(60)Co照射7 Gy并用 400 mg/kg 复方蟾皮胶囊治疗7 d,其瘤重抑制率达67.4％;BALB/c小鼠腹腔内接种10~6个/0.1ml S_(180)瘤细胞后全身照射 7 Gy,200 mg/kg复方蟾皮胶囊灌胃8d,腹水量和癌细胞总数分别为0.6±0.3ml(P<0.01)和1.845±1.253×10~7(P<0.01);小鼠S_(37)     

缺锌儿童外周血诱生α干扰素能力与自然杀伤细胞活性的研究

本文对100例缺锌患儿与健康儿童外周血诱生α干扰素(IFN-α)能力、自然杀伤细胞(NK)活性、发锌和全血锌含量以及它们之间的关系进行研究。结果显示:缺锌患儿发锌(57.4±6.2μg/g)、全血锌(5.8±0.9μg/ml),诱生IFN-α水平(1.82×10~6±5.02×10~4IU/L)和NK细胞活性(29.34+4.12％)均显著低于健康儿童(发锌: 110.5±8.1μg/g、全血锌:13.7±3.9μg/ml、NK细胞活性:38.42±2.21％,IFN-α效价:2.41×10~6±4.12×10~4 IU/L),其他元素铜、铁含量同诱生IFN-α能力、NK细胞活性之间无明显相关关系。提示锌缺乏可能是导致机体抗感染免疫功能低下而致使缺锌儿童对感染性疾病抵抗力降低的重要因素之一。     

人的正常卵巢及卵巢癌绒毛膜促性腺激素受体的研究

活检正常卵巢标本23例和卵巢癌标本16例(粘液性和浆液性囊腺癌各8例),分离细胞膜作绒毛膜促性腺激素受体结合反应。结果表明:正常卵巢、粘液性及浆液性卵巢癌之平均最大结合率分别为13.30±2.24％、20.15±5.14％及9.06±6.40％;受体量分别为0.66±0.14×10~(-10)mol/μg膜蛋白、1.97±1.24×10~(-10)mol/μg膜蛋白及0.48±0.10×10~(-10)mol/μg膜蛋白,Kd值分别为10.10±5.50×10~(-9)mol、25.00±0.29×10~(-9)mol及16.90±0.14×10~(-9)mol。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.