Pyrrole and indole alkaloids from an endophytic Fusarium incarnatum (HKI00504) isolated from the mangrove plant Aegiceras corniculatum

Two new pyrrole alkaloids, N-[4-(2-formyl-5-hydroxymethyl-pyrrol-1-yl)-butyl]-acetamide (1) and N-[5-(2-formyl-5-hydroxymethyl-pyrrol-1-yl)-pentyl]-acetamide (2), and a new indole derivative (3aR,8aR)-3a-acetoxyl-1,2,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol (3) were isolated, together with ( - )-3a-hydroxyfuroindoline, (3aR,8aS)-1-acetyl-1,3,3a,8,8a-hexahydropyrrolo-[2,3-b]indol-3a-ol, and N-acetyltryptamine A, from an endophytic ascomycetous fungus, Fusarium incarnatum (HKI00504), which was isolated from the mangrove plant Aegiceras corniculatum. The structures of compounds 1-3 were determined on the basis of extensive spectroscopic data analyses.     

New cyclopentenone derivatives from an endophytic Streptomyces sp. isolated from the mangrove plant Aegiceras comiculatum

From the mangrove plant Aegiceras comiculatum collected in South China an endophytic Streptomyces sp. was isolated. Following cultivation in a seawater-based medium, four new cyclopentenone derivatives, namely (5R) 3-amino-5-hydroxy-5-vinyl-2-cyclopenten-1-one (1), (5R) 5-hydroxy-3-[(methoxycarbonyl)amino]-5-vinyl-2-cyclopenten-1-one (2), (5R) 5-hydroxy-3-[[2-(4-hydroxyphenyl)ethyl]amino]-5-vinyl-2-cyclopenten-l-one (3), and 3-isobutylpropanamide-2-cyclopenten-l-one (4), were obtained from the fermentation broth. Their structures were elucidated by extensive spectroscopic (UV, IR, ESIMS, and 2D NMR) data analyses.     

海洋红树植物桐花树内生菌GT20021545发酵液的化学成分研究

目的对海洋红树植物桐花树内生菌GT20021545发酵液进行化学成分研究。方法采用树脂、硅胶、凝胶柱色谱及高效液相色谱等手段,结合其理化性质分离海洋红树植物桐花树内生菌GT20021545发酵液的化学成分,并运用波谱分析方法,对化合物的结构进行解析鉴定。结果与结论从内生菌GT20021545发酵液中分离得到10个化合物:4个吲哚类化合物,5个苯环类化合物,1个环二肽类化合物。     

桐花树多糖的提取及其组成的研究

目的本文对红树植物桐花树中多糖的提取及其组成进行了研究。方法桐花树叶经热水浸提、Sevage法脱蛋白等方法得到多糖;经完全酸水解,糖腈乙酸酯衍生化,并用红外光谱仪、气质联用仪对其结构和组成进行了研究。结果桐花树多糖由D-核糖、D-阿拉伯糖、D-木糖、D-甘露糖和D-葡萄糖组成。结论桐花树多糖为杂多糖。     

茅尾海桐花树根际土壤来源几丁质降解菌株多样性及抗植物真菌活性的研究

摘要：目的 以胶体几丁质为唯一碳源的培养基,从广西茅尾海红树林桐花树根际土壤中分离几丁质降解菌株,并应用复 筛培养基筛选产几丁质酶菌株,结合平板对峙法挖掘能高效抑制植物病原真菌生长的活性菌株,为红树林来源微生物农用生物 防治药物的研发奠定基础。方法 采用平板稀释涂布法和划线法分离纯化菌株,并应用复筛培养基通过透明圈观察法检测菌株 几丁质酶活;采用Chelex-100法提取菌株基因组DNA,通过PCR扩增菌株的16S rRNA基因序列,上传至EzBioCloud数据库进行 在线比对;采用平板对峙法筛选抗植物病原真菌的活性菌株;采用PKS和NRPS基因的扩增引物分别检测基因组聚酮合酶基因与 非核糖体肽合成酶基因。结果 从桐花树根际土壤中分离获得28株几丁质降解菌,隶属于10目10科17属;其中5株几丁质降解 菌对4种植物病原真菌均显示出抑菌活性,抑菌活性阳性率为17.86%;并在17株几丁质降解菌中检测到抗生素生物合成基因。 结论 广西茅尾海红树林桐花树根际土壤来源的细菌及放线菌是重要的几丁质酶菌种资源,它们在抗植物病原真菌方面表现出 良好的应用潜力,在新型绿色海洋生物农药、新颖结构海洋药物及其他高赋值产品开发和利用方面具有应用前景。     

Two terpenoids and a polyketide from the endophytic fungus Trichoderma sp. Xy24 isolated from mangrove plant Xylocarpus granatum

Three known compounds were isolated from the endophytic fungus Trichoderma sp. Xy24 from a mangrove plant Xylocarpus granatum by using various column chromatography techniques. Their structures were identified as harzianone (1), trichoacorenol (2), and trichodimerol (3) by extensive spectroscopic analysis. Among them, 1 was a harziane diterpene, 2 wasa sesquiterpene alcohol, and 3 was a polyketide with a completely symmetric configuration. Compound 3 exhibited medium inhibitory activity with an IC₅₀ value of 74.6 μMusing a NA (H7N9)/MUNANA model.     

红树植物老鼠簕内生真菌橘青霉P4-5-1中的1个新异喹啉酮生物碱

目的 研究老鼠簕内生真菌橘青霉(Penicillium citrinum)P4-5-1的化学成分。方法 利用正反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等技术对P4-5-1次生代谢产物进行分离,利用高分辨质谱、一维及二维核磁共振等波谱鉴定技术确定化合物结构。结果 从P4-5-1大米发酵物中分离得到了6个化合物,分别为4-methoxy-2-methylisoquinolin-1(2H)-one (1)、fischexanthone (2)、penicitrinone A (3)、ent-aspergoterpenin C (4)、sydowiol E (5)和citrinin (6)。结论 化合物1为新异喹啉酮生物碱,命名为citrinadin D;化合物2、4和5首次从该种真菌中获得。     

红树植物角果木内生真菌J62生物活性成分研究

目的 研究红树植物角果木内生真菌J62产生的活性次生代谢产物。方法 采用多种柱色谱技术进行分离纯化,根据理化常数和波谱数据鉴定化合物的结构,采用滤纸片琼脂扩散法测定化合物抗金黄色葡萄球菌(Staphylococcus aureus)和白色念珠菌(Candida albicans)活性,利用MTT法测定各化合物的体外细胞毒活性。 结果 从角果木内生真菌J62发酵液中分离鉴定了10个化合物,分别为：反式桂皮酸 (1),3,4-二甲氧基苯甲醇 (2), 2-[(3,4-dimethoxyphenyl)methyl]-4,5-dimethoxy-benzenemethanol (3), l尿嘧啶核苷(4), similin B (5),coibanol C (6), hymatoxin C (7), 5,8-epidioxy-5a,8a-ergosta-6,22E-dien-3b-ol (8),豆甾-7,22-二烯-3b,5a,6a-三醇 (9)和crispin A (10)。结论 生物活性测试结果表明,化合物1, 2和7具有抗金黄色葡萄球菌活性,化合物1和2具有抗白色念珠菌的活性;化合物2和5对肿瘤细胞株K-562显示有生长抑制活性。     

A new chloro-monoterpene from the mangrove endophytic fungus Tryblidiopycnis sp. (4275)

A new chloro-monoterpene (compound 1) and three known compounds, peroxyergosterol, uracil and methylisocoumarin, were isolated from the ethyl acetate extract of the fermentation broth of the mangrove endophytic fungus Tryblidiopycnis sp. (4275) obtained from Kandelia woody tissue from Mai Po, Hong Kong. Its structure was determined spectroscopically and by X-ray crystallographic analysis.     

A new chloro-monoterpene from the mangrove endophytic fungus Tryblidiopycnis sp. (4275)

A new chloro-monoterpene (compound 1) and three known compounds, peroxyergosterol, uracil and methylisocoumarin, were isolated from the ethyl acetate extract of the fermentation broth of the mangrove endophytic fungus Tryblidiopycnis sp. (4275) obtained from Kandelia woody tissue from Mai Po, Hong Kong. Its structure was determined spectroscopically and by X-ray crystallographic analysis.     

Antimicrobial activity and cytotoxicity of bis(indole) alkaloids from the sponge Spongosorites sp

Bis(indole) alkaloids, of the topsentin class (1-4) and hamacanthin class (5-9), isolated from the marine sponge Spongosorites sp. were investigated using several biological assays. In the evaluation of antimicrobial activity against various strains of bacteria and fungi, compounds of the hamacanthin class exhibited more potent antibacterial activity than those of the topsentin class. Deoxytopsentin (1) and hamacanthin A (5) also exhibited significant antibacterial activity against methicillin-resistant Staphylococcus aureus, with MIC values of less than 12.5 microg/ml. In the antifungal activity test, hamacanthins, especially hamacanthin A (5), showed potent inhibitory activity against medically important pathogenic fungi. In contrast, all of the topsentins (1-4) were inactive against fungal growth. These compounds (1-9) also exhibited moderate cytotoxicity against cancer cell lines at concentrations between 1.1 and >20 microg/ml.     

红树植物卤蕨内生真菌Penicillium sp.0935030中的抗菌活性成分研究

为研究红树林植物卤蕨（Acrostichum aureurm）内生真菌Penicillium sp．0935030发酵物中的抗菌活性成分，采用活性追踪的方法，用多种色谱技术对化合物进行分离纯化．通过理化性质和光谱数据鉴定出7个化合物，分别为：环（脯氨酸-苏氨酸）（1）．环（脯氨酸-酪氨酸）（2），1-呋喃基-1，2-乙二醇（3），（3p．4B，22B）-12-烯-3，22，23-三羟基-齐墩果烷（4），甘露醇（5），尿苷（6）和甘草素（7）。以上化合物均为首次从该真菌中分离得到。用滤纸片琼脂扩散法测定上述化合物的抗菌活性，其中化合物1、2和7具有明显抗金葡菌和耐甲氧西林金葡菌活性。     

Chemistry and biology of new marine alkaloids from the indole and annelated indole series

Chemistry and biology of marine natural products from the indole and annelated indole series have become an attractive research field for development of new pharmacological lead substances. In the past years some of the isolated natural organic compounds were synthesized by chemists and evaluated with great enthusiasm to find new lead natural compounds against different diseases. In this review the latest results for new compounds including isolation, biological evaluation, synthetic pathways and some retrosynthetic analyses are summarized.     

红树林植物海莲内生真菌Penicillium sp.091402化学成分的研究

目的 对红树植物海莲内生真茵茵丝体提取物的乙酸乙酯萃取部分进行化学成分研究.方法 采用硅胶柱色谱等分离手段,利用理化教据和各种波谱分析方法,结合文献对照,对海莲内生真菌penicilium sp.091402菌丝体中化学成分进行分离鉴定.结果与结论 从海莲内生真菌penicilium sp.091402菌丝体乙酸乙酯萃取部分分离得到6个化合物,分别鉴定为:(3β,22E)-麦角甾-5,7,22-三烯-3-醇(1),(313,5α,8α,22E)-5,8-过氧麦角甾-6,22-二烯-3-醇(2),(3β,5α,6α,7α,22E)-5,6-环氧麦角甾-8(14),22-=-烯-3,7-二醇(3),(3β,5α,6β,22E)-麦角甾-7,22-二烯-3,5,6-三醇(4),soyasapogenol B(5)和citrinin H2(6).     

红树植物角果木内生真菌Cladosporium sp. JS1-2的次级代谢产物研究

从红树植物角果木内生真菌枝孢霉属Cladosporium sp. JS1-2的发酵产物中共分离得到5个异香豆素类化合物(1~5),1个苯并呋喃衍生物(6),1个异色满衍生物(7),以及5个其他类化合物(8~12),通过理化性质和波谱数据对化合物的结构进行了鉴定。通过微量稀释法测定所有化合物的抗菌活性,其中化合物6~8和11对金黄色葡萄球菌(Staphylococcus     

红树植物角果木内生真菌Cladosporium sp.JS1-2的次级代谢产物研究

从红树植物角果木内生真菌枝孢霉属Cladosporium sp. JS1-2的发酵产物中共分离得到5个异香豆素类化合物(1～5),1个苯并呋喃衍生物(6),1个异色满衍生物(7),以及5个其他类化合物(8～12),通过理化性质和波谱数据对化合物的结构进行了鉴定。通过微量稀释法测定所有化合物的抗菌活性,其中化合物6～8和11对金黄色葡萄球菌(Staphylococcus aureus)的MIC值均为12.5μg/mL,阳性对照环丙沙星(ciprofloxacin)的MIC值为3.12μg/mL。同时对所有化合物进行棉铃虫幼虫生长抑制活性测试,结果表明化合物4、5、8、9和11对棉铃虫幼虫的IC50值均为100μg/mL,阳性对照印楝素(azadirachtin)的IC50值为50μg/mL。