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1.
Focal hyperplasia of intracytoplasmic filaments of 100Å in diameter was observed within chief cells of the parathyroid gland in dogs treated with nalidixic acid. The structure, as a rule, was located in the neighborhood of the nucleus and no other cell organelles were detected within it. Its size had a wide spectrum from a small part of the cytoplasm to as large as half a cell. Golgi apparatus and rough endoplasmic reticulum in the involved cells had a tendency to arophy. These cells occasionally contained less secretory granules. Lipid droplets are diffusely increased. The pathogenesis and significance of the present intracytoplasmic filaments remain to be determined. However, as lipid deposition, atrophy of Golgi apparatus, poorly developed rough endoplasmic reticulum occur in a hypofunctional or degenerative state, it might be possible that filamentous hyperplasia is closely associated with that state. 相似文献
2.
H. Warshawsky A. Haddad R. P. Gonalves V. Valeri F. L. De Lucca 《Developmental dynamics》1973,138(1):79-119
The South American rattlesnake venom gland is made up of secretory tubules lined by a simple columnar epithelium containing horizontal cells, mitochondria-rich cells, and the principal cell type, the columnar secretory cells. This cell has a round basal nucleus and abundant rough endoplasmic reticulum, the cisternae of which are variably distended with flocculent material containing many dense intracisternal granules. The supranuclear Golgi apparatus is spherical, with stacks of flattened saccules at the periphery and large vacuoles containing masses of dense material, and other dense granules in the center. Similar but smaller granules are present at the apex where they fuse with the microvillus-covered apical membrane and release their content into the lumen. Protein synthesis was studied in snakes injected with 3H-tyrosine and sacrificed at several times after injection. Radioautographs showed reactions at one half and one hour over the ribosomes and membranes of the rough endoplasmic reticulum. At two hours the immature face of the Golgi apparatus was labeled. At four hours Golgi saccules and vacuoles with dense masses (secretory granules) were labeled, and at eight hours the dense masses within the secretory granules were heavily labeled both in the Golgi region and in the apex near the lumen. Labeled material was found in the lumen at two days. Intracisternal granules were first labeled at eight hours, and by two days reactions remained only over the flocculent content and intracisternal granules of the rough endoplasmic reticulum. Thus, venom protein was synthesized on the rough endoplasmic reticulum, migrated through the Golgi apparatus and accumulated in the dense masses of the secretory granules, which moved to the apex and were extruded. The labeling of intracisternal granules at eight hours and two days after injection indicated a storage nature for these granules. 相似文献
3.
Taste buds in foliate papillae of the rhesus monkey were examined by electron microscopy. Three distinct cell types were identified. Type I cells were narrow elongated cells containing an oval nucleus, bundles of intermediate filaments, several Golgi bodies, and characteristic apical membrane-bounded dense granules. These cells exhibited morphological variations: some had a moderately dense cytoplasm, perinuclear free ribosomes, and flattened sacs of rough endoplasmic reticulum; others had a more lucent cytoplasm, dilated irregular rough endoplasmic reticulum, lysosome-like dense bodies, and lipid droplets. Type II cells typically contained a spherical, pale nucleus, a prominent nucleolus, supranuclear and infranuclear Golgi bodies, mitochondria with tubular cristae, and one or two centrioles. This cell type, too, showed some variation in the relative amounts of ribosomes and smooth endoplasmic reticulum, which varied inversely with each other. Type III cells were characterized by a clear apical cytoplasm essentially devoid of ribosomes and containing microtubules. In a few type III cells, the peri- and infranuclear regions contained many ribosomes and some rough endoplasmic reticulum. In most Type III cells, there were large numbers of dense and clear vesicles in the peri- and infranuclear regions; some of the vesicles were grouped in synapse-like arrangements with adjacent nerves. The morphological variations exhibited by all three cell types could be accounted for by age differences in each of the cells. This would be consistent with the notion that cell renewal occurs in each of the three cell populations. 相似文献
4.
Intimal changes at the prelesional stage of atherosclerotic lesions were investigated ultrastructurally using hamsters fed a high cholesterol diet for 1 day to 1 month. Observations were restricted to the lesion-prone area in the aortic arch. The endothelial cells began to show well-developed rough endoplasmic reticulum and Golgi apparatus after a few days of the cholesterol diet. After three days, macrophages which contained a few lipid droplets were observed just below the endothelium. We found that the intimal smooth muscle cell formed a gap junction with the process of the medial smooth muscle cell. After a few weeks to 1 month on the diet, the intima became markedly thickened and filled with dense extracellular matrices. The intimal smooth muscle cells showed well-developed rough endoplasmic reticulum and Golgi apparatus with immature granules, suggestive of high secretory activity. The present study showed that endothelial ultrastructural changes, macrophage invasion, and medial smooth muscle cell migration are very early events occurring within a few days after cholesterol intake commences. 相似文献
5.
大白鼠肥大心肌细胞的超微结构及细胞化学研究 总被引:1,自引:0,他引:1
结扎大白鼠腹主动脉,使其狭窄,造成心脏负荷增加,饲养7周后,引起左心室心肌肥大。电子显微镜下,肥大的心肌细胞核大,核染色质淡,核仁大;核糖体和粗面内质网增生;高尔基体数目增多,体积增大,线粒体、溶酶体等也增多。对照组心肌细胞核相对较小,粗面内质网稀少,高尔基体亦小。本实验还采用细胞化学G6Pase和TPPase,分别显示肌浆网和高尔基体形态。肥大的心肌细胞除了肌浆网小管有酶反应外,增生的粗面内质网腔内有G6Pase反应产物。在肥大心肌细胞高尔基体显示了TPPase反应,更证实了高尔基体体积增大以及数目增多。本实验动物的心肌细胞,正处在工作负荷增加,蛋白质合成旺盛的心肌细胞肥大代偿期。 相似文献
6.
Masayasu Kojimahara Mitsuo Koyama Ichizo Igari Genju Ooneda 《Pathology international》1981,31(2):277-288
A case of benign hemangioendothelioma which occurred in the hard palate was observed and its electron microscopic findings were described. The endothelial cells contained an abundance of cytoplasmic filaments, but they contained a small number of Weibel-Palade bodies. Two types of endothelial cells, light and dark, were found in our specimen. The former had a cytoplasm with an abundance of ribosomes, rough endoplasmic reticulum and scant filaments. The latter had a cytoplasm with numerous cytoplasmic filaments and a well-developed Golgi apparatus. Tight junctions were seen between the endothelial cells. The majority of the pericytes were accompanied with the vessels in the tumor. Three types of pericytes were recognized: endothelial cell-like, fibroblast-like, and smooth muscle cell-like pericytes. 相似文献
7.
M Yamamoto 《Archives of histology and cytology》1989,52(5):529-541
The d-segment of the rat penile bone at 14 weeks is composed of an outer zone of atrophy, a middle zone of hypertrophy and an inner zone of ossification. Hypertrophic chondrocytes in the middle zone generally present a few secretory vesicles and a poorly developed Golgi apparatus and rough endoplasmic reticulum, suggesting a serious decrease in the secretion of territorial matrix substances. Instead, most of the cells contain prominent glycogen lakes, lipid droplets and/or fine cytoplasmic filaments. These and other findings indicate that the chondrocytes are in a resting state. In the calcified layer of the zone, and in addition to the hypertrophic or degenerating chondrocytes, another type of cell is recognizable. These sometimes remain viable through the process of cartilage degeneration and may be liberated from the besieging calcified interterritorial matrix and from their own lacuna by chondroclasts. These surviving cells are more similar in ultrastructure to bone cells than to the adjacent chondrocytes, surrounded by the lamina limitans characteristic of the resting osteocytes. However, they are directly covered by a typical territorial matrix inside the lamina limitans and do not extend slender cell processes into the calcified matrix beyond the lamina limitans, and the territorial matrix is never calcified even after calcification of the interterritorial matrix. The cells, therefore, are regarded as belonging to the chondrocytes. 相似文献
8.
Somatotrophs from ten pituitary adenomas were evaluated morphometrically by light and electron microscopy using the following parameters: a) nuclear, cytoplasmic and cell volumes; b) volume density, total volume, surface density, total surface and surface/volume ratio of secretory granules, mitochondria, rough endoplasmic reticulum and Golgi apparatus, and c) the number of secretory granules and mitochondria per micron3 of cytoplasm and per cell. The results were compared (p less than 0.05 and p less than 0.10) with those obtained from somatotrophs identified in five normal pituitaries. The data obtained indicate that: a) in the adenomas, the number of secretory granules per cell cannot be accurately evaluated from their apparent number in sectioned cell profiles; b) there are two basic sub-types of adenomatous somatotrophs defined according to the mean secretory granule diameter; cells in which granule diameter is inferior to 180 nm exhibit distinct morphological features such as nuclear pleomorphism, the presence of gross bundles of intermediate sized filaments or fibrous bodies in the cytoplasm and a variable number of secretory granules. Adenomas constituted mainly by these cells were found in younger patients, suggesting the more aggressive nature of these tumours, thus warranting close clinical follow-up of such patients; and c) in both types of adenomatous cells, the organelles directly involved in the secretory process, i.e., the rough endoplasmic reticulum and Golgi apparatus, are larger than in the control cells; however, the ratio between the surfaces of these two compartments does not differ among the three groups studied. 相似文献
9.
A lectin (soybean agglutinin or SBA) conjugated with horseradish peroxidase was used to identify the intracellular membranes containing galactose or alpha-N-acetylgalactosamine in young chick ciliary ganglions. The label is found on one or, less frequently, on two saccules of the Golgi apparatus in the ciliary and choroid neurones. The labelled intermediate saccules are always located either near the cisface or the middle region of the Golgi apparatus. This observation confirms that distinct compartments are present in the Golgi apparatus. Labelling is also frequently observed at the end of the rough endoplasmic reticulum cisternae in the neurones. It is also sometimes observed in a few vesicles. The lectin-peroxidase labelling, however, was never observed in Schwann cell Golgi apparatus or in their rough endoplasmic reticulum. 相似文献
10.
Joel Schechter 《Developmental dynamics》1969,126(4):477-487
Stellate cells in the rabbit pars distalis have been studied electron microscopically from birth to adulthood. During this period the stellate cells are found in abundance throughout the pars distalis and are the only consistently agranular cells observed in the pars distalis parenchyma. Identifying features of stellate cells are fine cytoplasmic filaments and farreaching cytoplasmic processes. The cytoplasmic processes ramify between adjacent parenchymal cells and are connected to other stellate cells by desmosomes. The stellate cells, linked to one another by desmosomes, thus constitute a meshwork within which the granulated parenchymal cells reside. Processes of stellate cells are also found interposed between the parenchymal basal lamina and the granulated parenchymal cells. The cytoplasmic filaments are distributed throughout the cytoplasm and extend into the fine processes. The filaments do not appear to have any particular orientation to the plasma membrane. Compared to granulated cell types, the cytoplasmic organization of stellate cells is not complex. Mitochondria, smooth and granular endoplasmic reticulum, Golgi membranes, ribosomes, dense bodies and lipid droplets are distributed throughout the cytoplasm, but not in great numbers. Nuclear chromatin is uniformly distributed. A nucleolus may or may not be evident. The ultrastructural features of the stellate cells are in many respects unchanged from birth to adulthood. An exception is in one-to-two-day-old neonates when in some stellate cells the quantity of cytoplasmic filaments appears increased and there is hyperplasia of the endoplasmic reticulum and Golgi membranes. A similar hyperplasia of these stellate cell organelles can be produced experimentally in adult rabbits by administration of Metopirone. Though the morphological evidence supports a structural or metabolically supportive function for the stellate cell, an additional role as the possible source of adrenocorticotrophic hormone is also discussed. 相似文献
11.
Takao Senda Tomoko Yoshinaga-Hirabayashi 《Anatomical record (Hoboken, N.J. : 2007)》1998,251(3):339-345
Intracellular membrane–bounded organelles, such as the endoplasmic reticulum, Golgi apparatus, and mitochondrion, possess and maintain their shape and intrinsic relationship due to the nature of their membrane organization. To reveal the membranous attachments that support these shapes and relationships, we examined various kinds of cells by quick-freeze deep-etch electron microscopy. In the cisternae of the endoplasmic reticula, we found intermembrane bridges linking opposite membranes of the cisternae. Membranes of adjoining rough endoplasmic reticulum cisternae were linked by intermembrane bridges crossing a narrow cytoplasmic gap between cisternae. Intermembrane bridges were also found in and between the Golgi cisternae and in nuclear envelopes. Three kinds of intermembrane bridges were found within mitochondria: one linking between outer and inner mitochondrial membranes and the other two spanning the intracristal space and intercristal matrix space. The presence of intermembrane bridges within membrane organelles, except for those between rough endoplasmic reticulum cisternae, was seen in all cell types examined. Intermembrane bridges within membrane organelles provide a structural basis for the membrane organization of the organelles and thus may contribute to the functional integrity of the organelles. Anat. Rec. 251:339–345, 1998. © 1998 Wiley-Liss, Inc. 相似文献
12.
Stereological methods were applied to obtain morphometric data related to pancreatic islets of Langerhans in the normal rabbit. By light microscopy, it was found that 1 mm3 of pancreatic parenchyma contained 47.7 islets, constituting 2.2% of its volume. Approximately 69% of the islets had diameters less than 80 μm; 31% were greater than 80 μm. The former group of islets, however, composed only 13% of the volume of the endocrine pancreas and the latter group, 87%. Using electron microscopy, a unit volume of islet tissue was observed to consist of 86% beta cells, 7.7% alpha cells, and 2.2% delta cells. The average beta-cell volume was 1260 μm3 and its cytoplasm consisted of 52.6% matrix, 12.7% rough endoplasmic reticulum, 10.2% secretory granules, 7.8% mitochondria, and 3.3% Golgi apparatus. A typical beta cell contained 662 mitochondria, intermediate (10-nm) filaments whose length totalled 50 mm, and 9,200 secretory granules with a ratio of four mature granules to each immature or “pale” granule. Within alpha and beta cells, three parameters were used for the quantitation of organelles or their component parts: (1) volume; (2) surface; and (3) numerical densities. In the beta cell, the surface densities of rough endoplasmic reticulum and Golgi membranes exceeded, by two- or threefold, their counterparts in alpha cells. Similarly, the number of beta-cell mitochondria exceeded by 30% that of alpha cells; but beta-cell mitochondrial volume was twice that of the alpha cell, as were surface densities of both inner and outer mitochondrial membranes. Volume and surface densities of secretory granules within beta cells were half the values obtained for alpha cells. An alpha cell contained three times the number of granules present in a beta cell. 相似文献
13.
The coagulating gland of rodents, which is part of the prostatic complex, secretes components of semen. Although possessing some ultrastructural features of other exocrine glands, the mechanism of secretion by these cells has been problematic. In the present study the pathway, kinetics, and mode of secretion in the coagulating gland of the mouse were studied by light and electron microscope autoradiography at intervals between 10 minutes and 3 hours after injection of 3H-fucose. The majority of silver grains overlay the Golgi apparatus at the initial interval, but in addition, more than a third of the grains were associated with extremely distended cisternae of the rough endoplasmic reticulum. At later intervals, radioactivity of the Golgi apparatus and the endoplasmic reticulum declined, while labeling of secretory granules increased greatly. Luminal contents became labeled 1 hour after administration of precursor. The results indicate that the pathway for secretion of glycoproteins proceeds through the Golgi apparatus to secretory granules and the glandular lumen, as in many other cells. In particular, heavy labeling of secretory granules at later intervals indicates that merocrine secretion is the most likely mechanism in the coagulating gland. However, the unusual observation that a significant proportion of grains overlay the rough endoplasmic reticulum at the initial interval raises the possibility that some fucose is incorporated into glycoproteins in the endoplasmic reticulum, as has been reported for other cell types with similarly configured endoplasmic reticulum. 相似文献
14.
Frank J. Weaker 《Anatomical record (Hoboken, N.J. : 2007)》1977,187(1):11-28
The interstitial tissue of the testis of the nine-banded armadillo is composed of blood vessels, clusters of Leydig cells, the usual connective tissue elements, and a network of lymphatic sinusoids. The endothelial walls of the sinusoids are separated from the peritubular contractile cell layer surrounding the seminiferous tubules by a thin layer of collagen. The peritubular contractile cell is characterized by filaments and dense bodies within the cytoplasm, whereas the endothelial cells lack these structures. Within each cluster, several Leydig cells surround one or more blood vessels. Adjacent Leydig cells are joined by 2- to 3-nm wide gap junctions and desmosome-like specializations. The Leydig cell is polygonal in shape with an ovoid nucleus. The cell is characterized by an abundance of smooth endoplasmic reticulum which appears as sheets of membranes, concentric whorls around vacuoles, and a random tubular network. Only a few short cisternae of rough endoplasmic reticulum are observed. Centrioles are closely associated with the Golgi apparatus. Rod-like mitochondria with tubular cristae are scattered throughout the cytoplasm. In addition, the cells contain vacuoles resulting from lipid extraction, filaments, microtubules, and glycogen. The surfaces of the cell exposed to the intercellular spaces exhibit numerous pinocytotic vesicles and cell processes which indicate active movement of material across the plasma membrane. In comparison to other mammalian species, the ultrastructural organization of the interstitium and the fine structure of the Leydig cell of the armadillo resemble those of the guinea pig. 相似文献
15.
Mikihiro Shamoto 《Pathology international》1978,28(4):507-512
Mitochondria, rough endoplasmic reticulum, Golgi apparatus and other cytoplasmic organelles were found within the nuclei, not in 'nuclear pseudo-inclusions', of some cells in several hematopoietic disorders. Mitoses were fairly often observed in the atypical cells of these cases. We suggest that these intranuclear cytoplasmic organelles may be trapped in the nucleus during an abnormal mitosis. 相似文献
16.
The morphology of the thyroid gland of the woodchuck, Marmota monax, was studied during the four seasons of the year. In the spring the thyroid is extremely heterogeneous in appearance. Some follicular cells appear quite active. They contain a well defined Golgi apparatus, abundant large colloid droplets and pseudopodia but few, if any, apical vesicles. Other less active cells have poorly defined rough surfaced endoplasmic reticulum and lack a well developed Golgi apparatus. They do not contain apical vesicles or colloid droplets. Summer thyroids have uniformly small follicles which are lined by high cuboidal cells containing numerous mitochondria, apical vesicles, abundant rough surfaced endoplasmic reticulum, and lipid droplets but few colloid droplets. There is extensive lateral and basal infolding of the cytoplasmic membranes in these cells. In the fall and winter the follicles are larger than in the summer and contain more colloid. Numerous heterogeneous dense bodies appear in the cytoplasm of the follicular cells in the fall and increase in number in the winter when there is an obvious sparsity of such glycoprotein synthetic organelles as Golgi apparatus and rough surfaced endoplasmic reticulum. These morphologic changes are compared with previous studies of thyroid structure and function in other animals and are correlated with the seasonal physiologic activities of the woodchuck. 相似文献
17.
Tapan Kumar Bhattacharyya David Gordon Butler 《Anatomical record (Hoboken, N.J. : 2007)》1979,193(2):213-231
The ultrastructure of the adrenocortical homologue (AH) of the north American eel (Anguilla rostrata) was studied from freshwater and long-term (1.5 years) seawater (SW) adpated animals. The AH tissue situated in the wall of cardinal veins is surrounded by a thin layer of collagenous capsule; in the region away from the vein wall, parenchymal cells are separated by interstitial lacunae containing collagen bundles, capillaries, chromaffin cells and nerve fibers often applied closely to the surface of AH cells. The free surface of the cell near the vein wall, capillaries or interstitial space extends numerous slender microplicae. The cytoplasm is characterized by the presence of mitochondria with tubular cristae, a network of smooth endoplasmic reticulum, a few cisternae of rough surfaced endoplasmic reticulum, well developed Golgi apparatus, coated vesicles, centrioles, cilium, filaments, microtubules, dense bodies of variable nature and a scarcity of liposomes. The cell nuclei possess invaginated cytoplasmic pseudo-inclusions. Electron histochemical reaction for free cholesterol revealed the occurrence of needle-shaped crystals mainly associated with surface microplicae and smooth endoplasmic reticulum, which seems to be the major organelle for storage or synthesis of this steroid precursor. SW animals indicated ultrastructural signs of stimulated steroid synthesis and secretion, i.e., high degree of pseudo-follicle formation, increased electron density of mitochondria, greater abundance of lysosomal dense bodies, hyperactivity of Golgi apparatus and dilation of smooth endoplasmic reticulum tubules. Some SW fishes showed extensive deposition of osmiophilic inclusions in the mitochondria and stacks of elongated cup-shaped mitochondria. Chronic seawater acclimation enhances AH activity as judged by ultrastructural criteria with ultimate mitochondrial degeneration resulting possibly from prolonged cortisol hypersecretion; the latter may be linked with physiologic re-adjustment of ionic transfer mechanism in hyperosmotic medium. 相似文献
18.
Dyal N. P. Singh Edward J. H. Nathaniel 《Anatomical record (Hoboken, N.J. : 2007)》1977,189(3):413-431
The differentiation of the mitral cell perikaryon in postnatal rat olfactory bulb was studied with the light and electron microscope. At birth the mitral cell was distinguishable and occupied a definitive position in the mitral cell layer. The cell contained a large oval nucleus surrounded by a thin rim of cytoplasm. Ribosomes, free and clustered, were scattered in the cell cytoplasm. Rough endoplasmic reticulum was relatively scarce. The Golgi complexes were made up of stacks of smooth-surfaced cisternae and associated vesicles. In certain cases the Golgi complexes projected into cellular processes. Mitochondria were present in all regions of the cytoplasm and contained well developed cristae. At the end of the first week, the mitral cell had developed significantly in size, and the cytoplasm contained well-developed rough endoplasmic reticulum. The Golgi complexes were made up of several stacks of smooth-surfaced cisternae with the association of vesicles and electron dense bodies. The apical dendrites o mitral cells at this periiod had increased significantly in length. Subsequently, during the second and third week, the rough endoplasmic reticulum and Golgi complexes became well developed. Associated with the Golgi complexes were electron dense lysosomal bodies. At three weeks and in older cells it was observed that dense lysosomal bodies. At three weeks and in older cells it was observed that dense lipofuschin granules increased significantly. It is suggested that the mitral cell matures and differentiates earlier than cells in the cerebral cortex. 相似文献
19.
M Kojimahara 《Acta pathologica japonica》1986,36(10):1477-1485
Seven cases of cavernous hemangioma of the liver found accidentally at autopsy were studied by electron microscope. The structures of the hemangioma consisted of capillaries and the intervening stromal cells. The endothelial cells contained pinocytotic vesicles, scattered ribosomes, rough endoplasmic reticulum, and Golgi apparatus. A distinct basement membrane was present beneath the endothelial cells, and multilaminated arrangement of the basement membrane was sometimes observed beneath the endothelial cells. The endothelial cells also contained numerous cytoplasmic filaments, but contained sparse specific endothelial granules. The internal elastic lamina was hardly seen at all in the hemangioma. The stromal cells showed typical smooth muscle cells, modified smooth muscle cells, and fibroblast-like cells. Elastic tissue as well as collagen fibers was present within the intervening stroma. Neither adventitia nor nerve fibers were found in the cavernous hemangioma. It is thought that cavernous hemangioma of the liver may be of a hamartomatous nature. 相似文献
20.
J Blomfield J A Simson A M Martinez J R Martinez 《Experimental and molecular pathology》1983,38(2):170-182
Rat submandibular gland tissue pieces were stimulated in vitro for 30 min with a beta-adrenergic agent or a cyclic AMP analog to stimulate protein secretion, or with alpha-adrenergic or cholinergic agents or a Ca2+ ionophore to stimulate fluid secretion. Acinar cells were examined by transmission electron microscopy. In control tissue, acinar cells showed little evidence of secretory activity. The Golgi apparatus was sparse and was associated with a few small, immature secretory granules with fine fibrillar contents. Following secretory granule discharge stimulated by isoproterenol or dibutyryl cyclic AMP, acinar cells were constricted, and had extensive basolateral membrane folding and tightly packed rough endoplasmic reticulum. Golgi complexes were prominent and had multiple small granules with filamentous contents. After stimulation of fluid secretion by alpha-adrenergic agents (epinephrine, phenylephrine), or cholinergic agents (acetylcholine, carbachol, pilocarpine), or a Ca2+ ionophore (A23187), the Golgi apparatus had compact concave cisternae enclosing aggregates of tubulovesicles. Acinar cells were distended, basolateral membranes were expanded, and rough endoplasmic reticulum was dilated and vesiculated. 相似文献